Turicibacter sp. H121

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Erysipelotrichia; Erysipelotrichales; Turicibacteraceae; Turicibacter; unclassified Turicibacter

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2191 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A0X8G224|A0A0X8G224_9FIRM UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2 6-diaminopimelate ligase OS=Turicibacter sp. H121 OX=1712675 GN=murE PE=3 SV=1
MM1 pKa = 7.52KK2 pKa = 10.34KK3 pKa = 10.43FIVAMMTCLFMVFSLFGCSPANEE26 pKa = 4.23QEE28 pKa = 4.16SDD30 pKa = 3.75SNQAAQSEE38 pKa = 4.65NDD40 pKa = 3.49SSTSDD45 pKa = 3.27QEE47 pKa = 4.36EE48 pKa = 4.32NQTSIPEE55 pKa = 4.43DD56 pKa = 3.49NNSDD60 pKa = 3.94SNTDD64 pKa = 3.25STNKK68 pKa = 9.49TEE70 pKa = 5.4SDD72 pKa = 5.14DD73 pKa = 4.94SNQDD77 pKa = 3.53DD78 pKa = 4.7NSDD81 pKa = 3.89SNQTPNTQQRR91 pKa = 11.84ISRR94 pKa = 11.84LYY96 pKa = 10.78YY97 pKa = 10.19YY98 pKa = 10.24DD99 pKa = 3.95IEE101 pKa = 4.37QNQMFYY107 pKa = 11.02VDD109 pKa = 4.62KK110 pKa = 10.7EE111 pKa = 4.01VDD113 pKa = 3.97VIEE116 pKa = 4.28NAYY119 pKa = 10.69VNALTEE125 pKa = 4.1ALKK128 pKa = 10.77EE129 pKa = 4.1NQDD132 pKa = 3.25NKK134 pKa = 11.32DD135 pKa = 3.31FVTINEE141 pKa = 4.31DD142 pKa = 3.71TFVTSATLDD151 pKa = 3.65EE152 pKa = 4.71EE153 pKa = 4.94SGILNIYY160 pKa = 9.85FNEE163 pKa = 4.11SFHH166 pKa = 7.28LSTNLGSGIEE176 pKa = 4.05AGFIDD181 pKa = 5.12ALVNTYY187 pKa = 10.19GYY189 pKa = 10.33NYY191 pKa = 10.44NVDD194 pKa = 3.05KK195 pKa = 11.18VAIYY199 pKa = 9.47VQNKK203 pKa = 9.72LYY205 pKa = 10.29EE206 pKa = 4.37DD207 pKa = 3.66QRR209 pKa = 11.84GPMPDD214 pKa = 3.26GYY216 pKa = 11.16FKK218 pKa = 10.37VTAEE222 pKa = 4.13DD223 pKa = 3.51AVKK226 pKa = 11.13YY227 pKa = 8.61EE228 pKa = 4.06

Molecular weight:
25.79 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A0X1TKD4|A0A0X1TKD4_9FIRM Ribonuclease P protein component OS=Turicibacter sp. H121 OX=1712675 GN=rnpA PE=3 SV=1
MM1 pKa = 7.38KK2 pKa = 9.47RR3 pKa = 11.84TWQPNKK9 pKa = 9.68RR10 pKa = 11.84KK11 pKa = 9.52RR12 pKa = 11.84AKK14 pKa = 8.76THH16 pKa = 5.23GFRR19 pKa = 11.84ARR21 pKa = 11.84MATPGGRR28 pKa = 11.84NVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 8.98KK37 pKa = 10.18GRR39 pKa = 11.84KK40 pKa = 8.13VLCAA44 pKa = 3.83

Molecular weight:
5.18 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2191

0

2191

654515

37

1529

298.7

33.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.325 ± 0.056

1.102 ± 0.017

5.131 ± 0.04

7.42 ± 0.069

4.412 ± 0.048

6.237 ± 0.052

2.083 ± 0.024

8.242 ± 0.055

6.693 ± 0.038

10.156 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.823 ± 0.026

4.784 ± 0.04

3.22 ± 0.03

4.059 ± 0.042

3.731 ± 0.037

5.912 ± 0.042

5.525 ± 0.037

7.085 ± 0.053

0.799 ± 0.019

4.261 ± 0.042

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski