Turicibacter sp. H121
Average proteome isoelectric point is 6.11
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2191 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0X8G224|A0A0X8G224_9FIRM UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2 6-diaminopimelate ligase OS=Turicibacter sp. H121 OX=1712675 GN=murE PE=3 SV=1
MM1 pKa = 7.52 KK2 pKa = 10.34 KK3 pKa = 10.43 FIVAMMTCLFMVFSLFGCSPANEE26 pKa = 4.23 QEE28 pKa = 4.16 SDD30 pKa = 3.75 SNQAAQSEE38 pKa = 4.65 NDD40 pKa = 3.49 SSTSDD45 pKa = 3.27 QEE47 pKa = 4.36 EE48 pKa = 4.32 NQTSIPEE55 pKa = 4.43 DD56 pKa = 3.49 NNSDD60 pKa = 3.94 SNTDD64 pKa = 3.25 STNKK68 pKa = 9.49 TEE70 pKa = 5.4 SDD72 pKa = 5.14 DD73 pKa = 4.94 SNQDD77 pKa = 3.53 DD78 pKa = 4.7 NSDD81 pKa = 3.89 SNQTPNTQQRR91 pKa = 11.84 ISRR94 pKa = 11.84 LYY96 pKa = 10.78 YY97 pKa = 10.19 YY98 pKa = 10.24 DD99 pKa = 3.95 IEE101 pKa = 4.37 QNQMFYY107 pKa = 11.02 VDD109 pKa = 4.62 KK110 pKa = 10.7 EE111 pKa = 4.01 VDD113 pKa = 3.97 VIEE116 pKa = 4.28 NAYY119 pKa = 10.69 VNALTEE125 pKa = 4.1 ALKK128 pKa = 10.77 EE129 pKa = 4.1 NQDD132 pKa = 3.25 NKK134 pKa = 11.32 DD135 pKa = 3.31 FVTINEE141 pKa = 4.31 DD142 pKa = 3.71 TFVTSATLDD151 pKa = 3.65 EE152 pKa = 4.71 EE153 pKa = 4.94 SGILNIYY160 pKa = 9.85 FNEE163 pKa = 4.11 SFHH166 pKa = 7.28 LSTNLGSGIEE176 pKa = 4.05 AGFIDD181 pKa = 5.12 ALVNTYY187 pKa = 10.19 GYY189 pKa = 10.33 NYY191 pKa = 10.44 NVDD194 pKa = 3.05 KK195 pKa = 11.18 VAIYY199 pKa = 9.47 VQNKK203 pKa = 9.72 LYY205 pKa = 10.29 EE206 pKa = 4.37 DD207 pKa = 3.66 QRR209 pKa = 11.84 GPMPDD214 pKa = 3.26 GYY216 pKa = 11.16 FKK218 pKa = 10.37 VTAEE222 pKa = 4.13 DD223 pKa = 3.51 AVKK226 pKa = 11.13 YY227 pKa = 8.61 EE228 pKa = 4.06
Molecular weight: 25.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.7
IPC2_protein 3.783
IPC_protein 3.783
Toseland 3.567
ProMoST 3.91
Dawson 3.757
Bjellqvist 3.948
Wikipedia 3.681
Rodwell 3.605
Grimsley 3.478
Solomon 3.757
Lehninger 3.706
Nozaki 3.872
DTASelect 4.088
Thurlkill 3.617
EMBOSS 3.694
Sillero 3.897
Patrickios 1.163
IPC_peptide 3.757
IPC2_peptide 3.872
IPC2.peptide.svr19 3.809
Protein with the highest isoelectric point:
>tr|A0A0X1TKD4|A0A0X1TKD4_9FIRM Ribonuclease P protein component OS=Turicibacter sp. H121 OX=1712675 GN=rnpA PE=3 SV=1
MM1 pKa = 7.38 KK2 pKa = 9.47 RR3 pKa = 11.84 TWQPNKK9 pKa = 9.68 RR10 pKa = 11.84 KK11 pKa = 9.52 RR12 pKa = 11.84 AKK14 pKa = 8.76 THH16 pKa = 5.23 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATPGGRR28 pKa = 11.84 NVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 KK36 pKa = 8.98 KK37 pKa = 10.18 GRR39 pKa = 11.84 KK40 pKa = 8.13 VLCAA44 pKa = 3.83
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.406
IPC2_protein 10.877
IPC_protein 12.442
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.369
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.106
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.055
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2191
0
2191
654515
37
1529
298.7
33.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.325 ± 0.056
1.102 ± 0.017
5.131 ± 0.04
7.42 ± 0.069
4.412 ± 0.048
6.237 ± 0.052
2.083 ± 0.024
8.242 ± 0.055
6.693 ± 0.038
10.156 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.823 ± 0.026
4.784 ± 0.04
3.22 ± 0.03
4.059 ± 0.042
3.731 ± 0.037
5.912 ± 0.042
5.525 ± 0.037
7.085 ± 0.053
0.799 ± 0.019
4.261 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here