Rhodotorula taiwanensis
Average proteome isoelectric point is 6.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7012 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2S5B4A1|A0A2S5B4A1_9BASI Uncharacterized protein OS=Rhodotorula taiwanensis OX=741276 GN=BMF94_5462 PE=4 SV=1
MM1 pKa = 7.4 SVINLTDD8 pKa = 3.04 VTVQNNPAGFTDD20 pKa = 5.21 PYY22 pKa = 10.37 QFQITFEE29 pKa = 4.44 CVSPIQDD36 pKa = 3.53 DD37 pKa = 4.78 LEE39 pKa = 4.28 WKK41 pKa = 8.51 LTYY44 pKa = 10.43 VGSAEE49 pKa = 4.01 SDD51 pKa = 3.61 SYY53 pKa = 11.69 DD54 pKa = 4.17 QEE56 pKa = 6.29 LDD58 pKa = 3.15 TCMVGPVPVGINSFPFEE75 pKa = 4.0 AAAPLPSRR83 pKa = 11.84 IPSSDD88 pKa = 3.79 LIGVTVILLTCSYY101 pKa = 10.93 NDD103 pKa = 3.35 QEE105 pKa = 4.63 FVRR108 pKa = 11.84 IGYY111 pKa = 8.07 YY112 pKa = 10.85 VNTEE116 pKa = 3.93 YY117 pKa = 11.4 GDD119 pKa = 4.23 PEE121 pKa = 5.04 LKK123 pKa = 10.51 AQYY126 pKa = 9.73 DD127 pKa = 3.34 ASLAEE132 pKa = 4.35 DD133 pKa = 4.03 AEE135 pKa = 4.59 EE136 pKa = 4.56 KK137 pKa = 10.64 GIKK140 pKa = 10.38 APDD143 pKa = 3.61 PTQHH147 pKa = 6.42 IDD149 pKa = 3.14 KK150 pKa = 10.55 LVRR153 pKa = 11.84 NVLADD158 pKa = 3.5 KK159 pKa = 10.88 PRR161 pKa = 11.84 VTKK164 pKa = 10.16 FNIQWDD170 pKa = 4.45 TPVAAVPTMSSAPIGGVTSEE190 pKa = 4.54 ALAGAPAAAAPPPPFNPYY208 pKa = 9.6 SAAAA212 pKa = 3.79
Molecular weight: 22.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.789
IPC2_protein 3.999
IPC_protein 3.973
Toseland 3.757
ProMoST 4.113
Dawson 3.948
Bjellqvist 4.101
Wikipedia 3.872
Rodwell 3.795
Grimsley 3.668
Solomon 3.935
Lehninger 3.897
Nozaki 4.062
DTASelect 4.279
Thurlkill 3.808
EMBOSS 3.884
Sillero 4.088
Patrickios 1.926
IPC_peptide 3.935
IPC2_peptide 4.062
IPC2.peptide.svr19 3.967
Protein with the highest isoelectric point:
>tr|A0A2S5BBQ0|A0A2S5BBQ0_9BASI Uncharacterized protein OS=Rhodotorula taiwanensis OX=741276 GN=BMF94_2752 PE=4 SV=1
MM1 pKa = 8.1 DD2 pKa = 5.07 KK3 pKa = 11.24 GKK5 pKa = 11.15 GKK7 pKa = 8.98 ATEE10 pKa = 4.19 LQPISLGVTLVADD23 pKa = 4.54 GCPASEE29 pKa = 4.8 GDD31 pKa = 5.1 DD32 pKa = 3.75 LAPSQLSHH40 pKa = 5.8 VQAPATPPRR49 pKa = 11.84 ASPLSVTSRR58 pKa = 11.84 SSSFFDD64 pKa = 4.35 DD65 pKa = 3.89 EE66 pKa = 4.71 PVPSTSSTPPQLSRR80 pKa = 11.84 PAFVEE85 pKa = 4.18 LVEE88 pKa = 4.43 TCPTPPSAATTEE100 pKa = 3.98 SSRR103 pKa = 11.84 YY104 pKa = 8.65 RR105 pKa = 11.84 RR106 pKa = 11.84 RR107 pKa = 11.84 PLLQANPSLVSLALVNEE124 pKa = 4.49 IGDD127 pKa = 4.58 DD128 pKa = 3.76 DD129 pKa = 4.07 TPAAVNALDD138 pKa = 4.13 EE139 pKa = 4.87 RR140 pKa = 11.84 PHH142 pKa = 6.21 EE143 pKa = 5.33 LPSAWQNDD151 pKa = 3.4 THH153 pKa = 6.19 SRR155 pKa = 11.84 RR156 pKa = 11.84 RR157 pKa = 11.84 SRR159 pKa = 11.84 QSRR162 pKa = 11.84 RR163 pKa = 11.84 STDD166 pKa = 3.14 SARR169 pKa = 11.84 TAAPLSEE176 pKa = 4.41 LATAEE181 pKa = 4.11 LVASILLQGPQVAAASTADD200 pKa = 3.63 AQPHH204 pKa = 5.66 LRR206 pKa = 11.84 RR207 pKa = 11.84 PSLDD211 pKa = 3.12 TRR213 pKa = 11.84 HH214 pKa = 6.42 SSRR217 pKa = 11.84 LSNDD221 pKa = 3.01 PYY223 pKa = 11.04 GVSSRR228 pKa = 11.84 YY229 pKa = 9.18 EE230 pKa = 4.02 GSRR233 pKa = 11.84 KK234 pKa = 9.85 SPSLDD239 pKa = 3.05 LRR241 pKa = 11.84 QQSFSSSHH249 pKa = 5.24 SLRR252 pKa = 11.84 PLSTPQHH259 pKa = 6.28 RR260 pKa = 11.84 PSAPALLRR268 pKa = 11.84 TRR270 pKa = 11.84 PSTVTLVSWDD280 pKa = 4.15 LSHH283 pKa = 7.26 LPAPNTPRR291 pKa = 11.84 EE292 pKa = 4.13 RR293 pKa = 11.84 PFKK296 pKa = 10.73 VPFRR300 pKa = 11.84 SVFLPTSYY308 pKa = 11.04 LGGGKK313 pKa = 8.24 GTGLYY318 pKa = 10.47 GDD320 pKa = 3.55 PCGPIEE326 pKa = 4.18 RR327 pKa = 11.84 KK328 pKa = 9.58 VKK330 pKa = 10.42 RR331 pKa = 11.84 EE332 pKa = 3.42 RR333 pKa = 11.84 ARR335 pKa = 11.84 PSNASGGSVGLGEE348 pKa = 4.26 RR349 pKa = 11.84 WRR351 pKa = 11.84 RR352 pKa = 11.84 WSGRR356 pKa = 11.84 RR357 pKa = 11.84 SSAMGYY363 pKa = 10.18 GGRR366 pKa = 11.84 TSWDD370 pKa = 3.6 EE371 pKa = 3.97 ASSIPPSATEE381 pKa = 4.32 DD382 pKa = 3.46 VGSLARR388 pKa = 11.84 EE389 pKa = 4.23 TEE391 pKa = 4.25 RR392 pKa = 11.84 PPSRR396 pKa = 11.84 SSSLLRR402 pKa = 11.84 RR403 pKa = 11.84 FKK405 pKa = 10.64 RR406 pKa = 11.84 RR407 pKa = 11.84 SWLSMPSSCEE417 pKa = 3.62 VNGAPEE423 pKa = 4.01 RR424 pKa = 11.84 PNARR428 pKa = 11.84 RR429 pKa = 11.84 QSLTSALFGSRR440 pKa = 11.84 RR441 pKa = 11.84 SKK443 pKa = 10.71 AASKK447 pKa = 10.6 ASSEE451 pKa = 4.1 GLEE454 pKa = 4.06 TWVSVVVRR462 pKa = 4.49
Molecular weight: 49.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.35
IPC2_protein 9.443
IPC_protein 10.423
Toseland 10.613
ProMoST 10.54
Dawson 10.701
Bjellqvist 10.467
Wikipedia 10.95
Rodwell 10.73
Grimsley 10.745
Solomon 10.877
Lehninger 10.833
Nozaki 10.613
DTASelect 10.452
Thurlkill 10.613
EMBOSS 11.038
Sillero 10.643
Patrickios 10.409
IPC_peptide 10.877
IPC2_peptide 9.604
IPC2.peptide.svr19 8.772
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7012
0
7012
3853630
11
5360
549.6
59.62
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.484 ± 0.03
0.981 ± 0.009
5.79 ± 0.02
5.992 ± 0.023
3.232 ± 0.018
7.217 ± 0.025
2.222 ± 0.013
3.612 ± 0.019
4.029 ± 0.021
9.14 ± 0.03
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.695 ± 0.009
2.578 ± 0.012
6.932 ± 0.034
3.768 ± 0.02
6.985 ± 0.023
8.834 ± 0.036
5.826 ± 0.016
6.154 ± 0.019
1.243 ± 0.01
2.287 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here