Bacillus piezotolerans

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4395 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3D8GXG9|A0A3D8GXG9_9BACI Uncharacterized protein OS=Bacillus piezotolerans OX=2259171 GN=DRW41_04925 PE=4 SV=1
MM1 pKa = 7.84ANFFNLNSTSINLPYY16 pKa = 9.86IYY18 pKa = 10.43EE19 pKa = 4.01NLIVLLTQIIKK30 pKa = 10.6GKK32 pKa = 10.5NNFSDD37 pKa = 3.61YY38 pKa = 11.61DD39 pKa = 3.63FAQWCDD45 pKa = 2.81NFTIIFEE52 pKa = 4.6DD53 pKa = 4.49DD54 pKa = 3.37EE55 pKa = 4.82VSKK58 pKa = 9.95NTEE61 pKa = 3.92HH62 pKa = 6.61VLLIAGDD69 pKa = 4.81IEE71 pKa = 4.58CQWDD75 pKa = 3.8LFLVNTYY82 pKa = 10.5SIEE85 pKa = 4.04EE86 pKa = 4.21LQKK89 pKa = 10.17MDD91 pKa = 3.61LTT93 pKa = 4.47

Molecular weight: 10.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3D8GS02|A0A3D8GS02_9BACI Uncharacterized protein OS=Bacillus piezotolerans OX=2259171 GN=DRW41_10325 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.54QPKK8 pKa = 9.44KK9 pKa = 7.96RR10 pKa = 11.84KK11 pKa = 8.69HH12 pKa = 5.94SKK14 pKa = 8.47VHH16 pKa = 5.85GFRR19 pKa = 11.84SRR21 pKa = 11.84MSSANGRR28 pKa = 11.84KK29 pKa = 8.7VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.39GRR39 pKa = 11.84KK40 pKa = 8.52VLSAA44 pKa = 4.05

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4395

0

4395

1252151

12

3039

284.9

31.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.765 ± 0.044

0.724 ± 0.013

4.936 ± 0.031

7.441 ± 0.04

4.693 ± 0.031

7.657 ± 0.037

1.911 ± 0.017

7.535 ± 0.039

6.876 ± 0.033

9.783 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.752 ± 0.018

4.195 ± 0.031

3.9 ± 0.021

3.17 ± 0.023

4.394 ± 0.03

5.915 ± 0.028

5.094 ± 0.025

6.805 ± 0.032

1.035 ± 0.014

3.419 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski