Amino acid dipepetide frequency for Bacillus piezotolerans

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.64AlaAla: 7.64 ± 0.11	0.617AlaCys: 0.617 ± 0.023	3.694AlaAsp: 3.694 ± 0.06	5.421AlaGlu: 5.421 ± 0.073	3.544AlaPhe: 3.544 ± 0.053	6.875AlaGly: 6.875 ± 0.084	1.273AlaHis: 1.273 ± 0.035	6.316AlaIle: 6.316 ± 0.079	4.97AlaLys: 4.97 ± 0.072	7.621AlaLeu: 7.621 ± 0.084	2.211AlaMet: 2.211 ± 0.045	2.893AlaAsn: 2.893 ± 0.052	2.358AlaPro: 2.358 ± 0.052	2.075AlaGln: 2.075 ± 0.046	3.286AlaArg: 3.286 ± 0.062	4.463AlaSer: 4.463 ± 0.06	3.349AlaThr: 3.349 ± 0.055	5.97AlaVal: 5.97 ± 0.084	0.692AlaTrp: 0.692 ± 0.023	2.378AlaTyr: 2.378 ± 0.042	0.0AlaXaa: 0.0 ± 0.0
Cys	0.45CysAla: 0.45 ± 0.02	0.099CysCys: 0.099 ± 0.01	0.351CysAsp: 0.351 ± 0.02	0.458CysGlu: 0.458 ± 0.022	0.335CysPhe: 0.335 ± 0.02	0.767CysGly: 0.767 ± 0.027	0.236CysHis: 0.236 ± 0.022	0.526CysIle: 0.526 ± 0.023	0.365CysLys: 0.365 ± 0.017	0.692CysLeu: 0.692 ± 0.024	0.157CysMet: 0.157 ± 0.009	0.251CysAsn: 0.251 ± 0.013	0.4CysPro: 0.4 ± 0.019	0.222CysGln: 0.222 ± 0.014	0.327CysArg: 0.327 ± 0.016	0.511CysSer: 0.511 ± 0.023	0.381CysThr: 0.381 ± 0.018	0.405CysVal: 0.405 ± 0.019	0.072CysTrp: 0.072 ± 0.008	0.233CysTyr: 0.233 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	3.449AspAla: 3.449 ± 0.062	0.382AspCys: 0.382 ± 0.019	2.191AspAsp: 2.191 ± 0.052	4.105AspGlu: 4.105 ± 0.062	2.511AspPhe: 2.511 ± 0.051	3.821AspGly: 3.821 ± 0.067	0.929AspHis: 0.929 ± 0.03	3.875AspIle: 3.875 ± 0.053	3.06AspLys: 3.06 ± 0.053	4.765AspLeu: 4.765 ± 0.072	1.298AspMet: 1.298 ± 0.035	1.727AspAsn: 1.727 ± 0.042	2.151AspPro: 2.151 ± 0.041	1.485AspGln: 1.485 ± 0.038	2.382AspArg: 2.382 ± 0.044	2.812AspSer: 2.812 ± 0.049	2.274AspThr: 2.274 ± 0.042	3.48AspVal: 3.48 ± 0.062	0.642AspTrp: 0.642 ± 0.021	2.024AspTyr: 2.024 ± 0.039	0.0AspXaa: 0.0 ± 0.0
Glu	5.867GluAla: 5.867 ± 0.072	0.405GluCys: 0.405 ± 0.021	3.46GluAsp: 3.46 ± 0.056	6.597GluGlu: 6.597 ± 0.095	2.944GluPhe: 2.944 ± 0.051	4.842GluGly: 4.842 ± 0.061	1.268GluHis: 1.268 ± 0.035	5.499GluIle: 5.499 ± 0.075	6.637GluLys: 6.637 ± 0.081	7.111GluLeu: 7.111 ± 0.098	2.315GluMet: 2.315 ± 0.042	3.618GluAsn: 3.618 ± 0.062	2.107GluPro: 2.107 ± 0.041	2.654GluGln: 2.654 ± 0.05	3.728GluArg: 3.728 ± 0.062	3.52GluSer: 3.52 ± 0.06	3.984GluThr: 3.984 ± 0.057	4.709GluVal: 4.709 ± 0.067	0.825GluTrp: 0.825 ± 0.027	2.32GluTyr: 2.32 ± 0.047	0.0GluXaa: 0.0 ± 0.0
Phe	3.411PheAla: 3.411 ± 0.065	0.403PheCys: 0.403 ± 0.02	2.351PheAsp: 2.351 ± 0.048	2.918PheGlu: 2.918 ± 0.047	2.59PhePhe: 2.59 ± 0.065	3.747PheGly: 3.747 ± 0.057	0.944PheHis: 0.944 ± 0.029	3.714PheIle: 3.714 ± 0.068	2.557PheLys: 2.557 ± 0.04	4.874PheLeu: 4.874 ± 0.078	1.251PheMet: 1.251 ± 0.034	1.901PheAsn: 1.901 ± 0.039	1.807PhePro: 1.807 ± 0.044	1.407PheGln: 1.407 ± 0.029	1.853PheArg: 1.853 ± 0.041	3.385PheSer: 3.385 ± 0.059	2.58PheThr: 2.58 ± 0.05	2.932PheVal: 2.932 ± 0.051	0.518PheTrp: 0.518 ± 0.021	1.787PheTyr: 1.787 ± 0.048	0.0PheXaa: 0.0 ± 0.0
Gly	5.433GlyAla: 5.433 ± 0.079	0.652GlyCys: 0.652 ± 0.021	3.349GlyAsp: 3.349 ± 0.057	5.064GlyGlu: 5.064 ± 0.071	3.765GlyPhe: 3.765 ± 0.066	5.672GlyGly: 5.672 ± 0.104	1.513GlyHis: 1.513 ± 0.038	6.581GlyIle: 6.581 ± 0.08	5.91GlyLys: 5.91 ± 0.078	7.236GlyLeu: 7.236 ± 0.084	2.352GlyMet: 2.352 ± 0.046	3.17GlyAsn: 3.17 ± 0.054	2.087GlyPro: 2.087 ± 0.043	2.444GlyGln: 2.444 ± 0.058	3.362GlyArg: 3.362 ± 0.057	4.303GlySer: 4.303 ± 0.064	4.603GlyThr: 4.603 ± 0.068	5.105GlyVal: 5.105 ± 0.07	0.936GlyTrp: 0.936 ± 0.03	3.035GlyTyr: 3.035 ± 0.057	0.0GlyXaa: 0.0 ± 0.0
His	1.247HisAla: 1.247 ± 0.034	0.198HisCys: 0.198 ± 0.013	0.919HisAsp: 0.919 ± 0.029	1.311HisGlu: 1.311 ± 0.033	1.007HisPhe: 1.007 ± 0.027	1.458HisGly: 1.458 ± 0.036	0.537HisHis: 0.537 ± 0.022	1.35HisIle: 1.35 ± 0.029	0.973HisLys: 0.973 ± 0.027	1.875HisLeu: 1.875 ± 0.04	0.47HisMet: 0.47 ± 0.019	0.72HisAsn: 0.72 ± 0.023	1.113HisPro: 1.113 ± 0.029	0.645HisGln: 0.645 ± 0.023	0.876HisArg: 0.876 ± 0.028	1.225HisSer: 1.225 ± 0.038	0.942HisThr: 0.942 ± 0.032	1.227HisVal: 1.227 ± 0.032	0.215HisTrp: 0.215 ± 0.014	0.802HisTyr: 0.802 ± 0.028	0.001HisXaa: 0.001 ± 0.001
Ile	6.222IleAla: 6.222 ± 0.081	0.599IleCys: 0.599 ± 0.021	4.075IleAsp: 4.075 ± 0.063	5.413IleGlu: 5.413 ± 0.074	3.267IlePhe: 3.267 ± 0.065	6.101IleGly: 6.101 ± 0.084	1.526IleHis: 1.526 ± 0.035	5.546IleIle: 5.546 ± 0.084	4.36IleLys: 4.36 ± 0.056	7.351IleLeu: 7.351 ± 0.101	1.771IleMet: 1.771 ± 0.041	2.902IleAsn: 2.902 ± 0.051	3.43IlePro: 3.43 ± 0.053	2.445IleGln: 2.445 ± 0.048	3.395IleArg: 3.395 ± 0.059	4.848IleSer: 4.848 ± 0.065	4.004IleThr: 4.004 ± 0.057	5.201IleVal: 5.201 ± 0.069	0.644IleTrp: 0.644 ± 0.027	2.251IleTyr: 2.251 ± 0.049	0.0IleXaa: 0.0 ± 0.0
Lys	5.235LysAla: 5.235 ± 0.075	0.335LysCys: 0.335 ± 0.018	3.762LysAsp: 3.762 ± 0.069	6.52LysGlu: 6.52 ± 0.077	2.032LysPhe: 2.032 ± 0.042	4.951LysGly: 4.951 ± 0.067	1.098LysHis: 1.098 ± 0.031	4.635LysIle: 4.635 ± 0.064	5.826LysLys: 5.826 ± 0.078	5.923LysLeu: 5.923 ± 0.071	2.158LysMet: 2.158 ± 0.04	3.38LysAsn: 3.38 ± 0.059	2.485LysPro: 2.485 ± 0.048	2.486LysGln: 2.486 ± 0.039	3.326LysArg: 3.326 ± 0.046	3.431LysSer: 3.431 ± 0.049	3.666LysThr: 3.666 ± 0.062	4.596LysVal: 4.596 ± 0.072	0.797LysTrp: 0.797 ± 0.026	2.12LysTyr: 2.12 ± 0.046	0.001LysXaa: 0.001 ± 0.001
Leu	8.316LeuAla: 8.316 ± 0.098	0.667LeuCys: 0.667 ± 0.025	4.924LeuAsp: 4.924 ± 0.068	6.789LeuGlu: 6.789 ± 0.091	5.009LeuPhe: 5.009 ± 0.082	7.061LeuGly: 7.061 ± 0.088	1.787LeuHis: 1.787 ± 0.039	6.87LeuIle: 6.87 ± 0.104	6.875LeuLys: 6.875 ± 0.083	10.283LeuLeu: 10.283 ± 0.14	2.462LeuMet: 2.462 ± 0.057	4.085LeuAsn: 4.085 ± 0.061	4.091LeuPro: 4.091 ± 0.059	2.965LeuGln: 2.965 ± 0.055	3.862LeuArg: 3.862 ± 0.065	6.58LeuSer: 6.58 ± 0.086	5.031LeuThr: 5.031 ± 0.06	6.31LeuVal: 6.31 ± 0.094	0.797LeuTrp: 0.797 ± 0.027	3.064LeuTyr: 3.064 ± 0.048	0.0LeuXaa: 0.0 ± 0.0
Met	2.55MetAla: 2.55 ± 0.045	0.145MetCys: 0.145 ± 0.011	1.608MetAsp: 1.608 ± 0.036	2.156MetGlu: 2.156 ± 0.041	1.069MetPhe: 1.069 ± 0.035	2.019MetGly: 2.019 ± 0.046	0.426MetHis: 0.426 ± 0.019	1.878MetIle: 1.878 ± 0.046	2.34MetLys: 2.34 ± 0.048	2.518MetLeu: 2.518 ± 0.048	0.814MetMet: 0.814 ± 0.031	1.368MetAsn: 1.368 ± 0.037	1.18MetPro: 1.18 ± 0.033	0.758MetGln: 0.758 ± 0.022	1.147MetArg: 1.147 ± 0.032	1.438MetSer: 1.438 ± 0.036	1.387MetThr: 1.387 ± 0.029	1.818MetVal: 1.818 ± 0.043	0.183MetTrp: 0.183 ± 0.013	0.717MetTyr: 0.717 ± 0.023	0.0MetXaa: 0.0 ± 0.0
Asn	2.837AsnAla: 2.837 ± 0.051	0.319AsnCys: 0.319 ± 0.015	1.914AsnAsp: 1.914 ± 0.051	3.274AsnGlu: 3.274 ± 0.058	1.664AsnPhe: 1.664 ± 0.035	3.655AsnGly: 3.655 ± 0.068	0.876AsnHis: 0.876 ± 0.026	2.927AsnIle: 2.927 ± 0.057	2.686AsnLys: 2.686 ± 0.048	3.766AsnLeu: 3.766 ± 0.057	1.131AsnMet: 1.131 ± 0.034	1.765AsnAsn: 1.765 ± 0.045	2.477AsnPro: 2.477 ± 0.055	1.504AsnGln: 1.504 ± 0.041	2.096AsnArg: 2.096 ± 0.042	2.302AsnSer: 2.302 ± 0.045	2.0AsnThr: 2.0 ± 0.04	2.791AsnVal: 2.791 ± 0.055	0.477AsnTrp: 0.477 ± 0.019	1.489AsnTyr: 1.489 ± 0.038	0.0AsnXaa: 0.0 ± 0.0
Pro	3.037ProAla: 3.037 ± 0.052	0.236ProCys: 0.236 ± 0.014	2.28ProAsp: 2.28 ± 0.047	3.512ProGlu: 3.512 ± 0.053	2.152ProPhe: 2.152 ± 0.053	3.163ProGly: 3.163 ± 0.054	0.788ProHis: 0.788 ± 0.026	2.765ProIle: 2.765 ± 0.049	2.239ProLys: 2.239 ± 0.04	3.625ProLeu: 3.625 ± 0.061	0.83ProMet: 0.83 ± 0.03	1.588ProAsn: 1.588 ± 0.037	1.262ProPro: 1.262 ± 0.031	1.079ProGln: 1.079 ± 0.028	1.278ProArg: 1.278 ± 0.033	2.414ProSer: 2.414 ± 0.043	1.749ProThr: 1.749 ± 0.032	3.168ProVal: 3.168 ± 0.051	0.378ProTrp: 0.378 ± 0.019	1.453ProTyr: 1.453 ± 0.041	0.0ProXaa: 0.0 ± 0.0
Gln	2.601GlnAla: 2.601 ± 0.044	0.18GlnCys: 0.18 ± 0.015	1.371GlnAsp: 1.371 ± 0.033	2.374GlnGlu: 2.374 ± 0.045	1.471GlnPhe: 1.471 ± 0.034	2.056GlnGly: 2.056 ± 0.042	0.579GlnHis: 0.579 ± 0.025	2.216GlnIle: 2.216 ± 0.045	2.365GlnLys: 2.365 ± 0.045	3.359GlnLeu: 3.359 ± 0.058	0.962GlnMet: 0.962 ± 0.032	1.371GlnAsn: 1.371 ± 0.035	1.228GlnPro: 1.228 ± 0.033	1.289GlnGln: 1.289 ± 0.039	1.388GlnArg: 1.388 ± 0.035	1.797GlnSer: 1.797 ± 0.041	1.6GlnThr: 1.6 ± 0.042	1.993GlnVal: 1.993 ± 0.047	0.368GlnTrp: 0.368 ± 0.017	1.131GlnTyr: 1.131 ± 0.029	0.0GlnXaa: 0.0 ± 0.0
Arg	2.818ArgAla: 2.818 ± 0.048	0.291ArgCys: 0.291 ± 0.017	2.322ArgAsp: 2.322 ± 0.052	3.717ArgGlu: 3.717 ± 0.072	2.096ArgPhe: 2.096 ± 0.045	2.762ArgGly: 2.762 ± 0.053	0.902ArgHis: 0.902 ± 0.03	3.421ArgIle: 3.421 ± 0.056	3.544ArgLys: 3.544 ± 0.065	4.419ArgLeu: 4.419 ± 0.065	1.343ArgMet: 1.343 ± 0.036	2.065ArgAsn: 2.065 ± 0.043	1.515ArgPro: 1.515 ± 0.044	1.57ArgGln: 1.57 ± 0.038	2.128ArgArg: 2.128 ± 0.052	2.197ArgSer: 2.197 ± 0.044	2.123ArgThr: 2.123 ± 0.048	2.766ArgVal: 2.766 ± 0.051	0.452ArgTrp: 0.452 ± 0.021	1.487ArgTyr: 1.487 ± 0.038	0.0ArgXaa: 0.0 ± 0.0
Ser	4.216SerAla: 4.216 ± 0.06	0.422SerCys: 0.422 ± 0.022	2.666SerAsp: 2.666 ± 0.046	3.746SerGlu: 3.746 ± 0.049	3.279SerPhe: 3.279 ± 0.053	5.076SerGly: 5.076 ± 0.068	1.18SerHis: 1.18 ± 0.027	4.724SerIle: 4.724 ± 0.071	3.615SerLys: 3.615 ± 0.052	6.248SerLeu: 6.248 ± 0.076	1.724SerMet: 1.724 ± 0.039	2.116SerAsn: 2.116 ± 0.043	2.423SerPro: 2.423 ± 0.043	1.812SerGln: 1.812 ± 0.037	2.587SerArg: 2.587 ± 0.05	3.759SerSer: 3.759 ± 0.059	2.79SerThr: 2.79 ± 0.054	4.004SerVal: 4.004 ± 0.053	0.657SerTrp: 0.657 ± 0.022	2.1SerTyr: 2.1 ± 0.042	0.001SerXaa: 0.001 ± 0.001
Thr	4.284ThrAla: 4.284 ± 0.064	0.322ThrCys: 0.322 ± 0.017	2.528ThrAsp: 2.528 ± 0.045	3.215ThrGlu: 3.215 ± 0.051	2.441ThrPhe: 2.441 ± 0.044	4.567ThrGly: 4.567 ± 0.062	0.994ThrHis: 0.994 ± 0.026	4.174ThrIle: 4.174 ± 0.066	2.987ThrLys: 2.987 ± 0.053	4.864ThrLeu: 4.864 ± 0.067	1.299ThrMet: 1.299 ± 0.036	2.116ThrAsn: 2.116 ± 0.046	2.212ThrPro: 2.212 ± 0.046	1.171ThrGln: 1.171 ± 0.037	1.941ThrArg: 1.941 ± 0.044	2.854ThrSer: 2.854 ± 0.055	2.45ThrThr: 2.45 ± 0.05	4.265ThrVal: 4.265 ± 0.067	0.503ThrTrp: 0.503 ± 0.02	1.756ThrTyr: 1.756 ± 0.034	0.0ThrXaa: 0.0 ± 0.0
Val	5.11ValAla: 5.11 ± 0.065	0.605ValCys: 0.605 ± 0.023	3.344ValAsp: 3.344 ± 0.054	4.654ValGlu: 4.654 ± 0.06	3.377ValPhe: 3.377 ± 0.058	4.56ValGly: 4.56 ± 0.064	1.283ValHis: 1.283 ± 0.03	5.261ValIle: 5.261 ± 0.077	4.571ValLys: 4.571 ± 0.062	6.757ValLeu: 6.757 ± 0.085	1.814ValMet: 1.814 ± 0.038	2.963ValAsn: 2.963 ± 0.046	2.895ValPro: 2.895 ± 0.049	2.142ValGln: 2.142 ± 0.042	2.824ValArg: 2.824 ± 0.054	4.521ValSer: 4.521 ± 0.065	3.754ValThr: 3.754 ± 0.066	4.61ValVal: 4.61 ± 0.081	0.667ValTrp: 0.667 ± 0.022	2.342ValTyr: 2.342 ± 0.049	0.0ValXaa: 0.0 ± 0.0
Trp	0.751TrpAla: 0.751 ± 0.026	0.077TrpCys: 0.077 ± 0.008	0.545TrpAsp: 0.545 ± 0.022	0.727TrpGlu: 0.727 ± 0.027	0.506TrpPhe: 0.506 ± 0.021	0.761TrpGly: 0.761 ± 0.027	0.215TrpHis: 0.215 ± 0.012	0.752TrpIle: 0.752 ± 0.024	0.752TrpLys: 0.752 ± 0.029	1.139TrpLeu: 1.139 ± 0.033	0.323TrpMet: 0.323 ± 0.016	0.534TrpAsn: 0.534 ± 0.022	0.284TrpPro: 0.284 ± 0.017	0.325TrpGln: 0.325 ± 0.018	0.432TrpArg: 0.432 ± 0.02	0.55TrpSer: 0.55 ± 0.021	0.508TrpThr: 0.508 ± 0.02	0.673TrpVal: 0.673 ± 0.025	0.141TrpTrp: 0.141 ± 0.013	0.354TrpTyr: 0.354 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.176TyrAla: 2.176 ± 0.04	0.282TyrCys: 0.282 ± 0.016	1.707TyrAsp: 1.707 ± 0.04	2.438TyrGlu: 2.438 ± 0.046	1.872TyrPhe: 1.872 ± 0.038	2.657TyrGly: 2.657 ± 0.054	0.756TyrHis: 0.756 ± 0.025	2.324TyrIle: 2.324 ± 0.051	2.133TyrLys: 2.133 ± 0.047	3.402TyrLeu: 3.402 ± 0.052	0.832TyrMet: 0.832 ± 0.03	1.415TyrAsn: 1.415 ± 0.039	1.49TyrPro: 1.49 ± 0.037	1.222TyrGln: 1.222 ± 0.032	1.72TyrArg: 1.72 ± 0.038	2.233TyrSer: 2.233 ± 0.048	1.767TyrThr: 1.767 ± 0.038	2.03TyrVal: 2.03 ± 0.04	0.386TyrTrp: 0.386 ± 0.019	1.348TyrTyr: 1.348 ± 0.034	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.001XaaAsn: 0.001 ± 0.001	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.001XaaSer: 0.001 ± 0.001	0.0XaaThr: 0.0 ± 0.0	0.001XaaVal: 0.001 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4395 proteins (1252151 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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