Spiroplasma chinense

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Entomoplasmatales; Spiroplasmataceae; Spiroplasma

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1218 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B9Y3T5|A0A5B9Y3T5_9MOLU RDD domain-containing protein OS=Spiroplasma chinense OX=216932 GN=SCHIN_v1c01410 PE=4 SV=1
MM1 pKa = 7.71EE2 pKa = 5.13KK3 pKa = 10.55CQLNNCGKK11 pKa = 10.0DD12 pKa = 3.69VNSLDD17 pKa = 3.61PKK19 pKa = 10.39RR20 pKa = 11.84LYY22 pKa = 10.28IYY24 pKa = 10.6DD25 pKa = 4.0EE26 pKa = 5.06LIEE29 pKa = 4.98DD30 pKa = 5.24EE31 pKa = 4.92IPVIVCDD38 pKa = 3.47SCYY41 pKa = 10.53DD42 pKa = 3.63AEE44 pKa = 4.26KK45 pKa = 10.72QADD48 pKa = 3.63NDD50 pKa = 3.93IDD52 pKa = 3.54WSHH55 pKa = 7.06SVDD58 pKa = 3.37GDD60 pKa = 3.7EE61 pKa = 4.91

Molecular weight: 7.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B9Y634|A0A5B9Y634_9MOLU Uncharacterized protein OS=Spiroplasma chinense OX=216932 GN=SCHIN_v1c10700 PE=4 SV=1
MM1 pKa = 7.32KK2 pKa = 9.47RR3 pKa = 11.84TWQPSKK9 pKa = 10.03IKK11 pKa = 10.14HH12 pKa = 5.53ARR14 pKa = 11.84THH16 pKa = 5.78GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.41NGRR28 pKa = 11.84KK29 pKa = 9.2VIKK32 pKa = 10.14ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.34GRR39 pKa = 11.84AKK41 pKa = 10.69LSAA44 pKa = 3.92

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1218

0

1218

414899

29

1769

340.6

38.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.304 ± 0.071

0.689 ± 0.017

5.634 ± 0.061

7.386 ± 0.075

5.574 ± 0.07

5.408 ± 0.07

1.17 ± 0.025

9.138 ± 0.078

9.569 ± 0.077

9.199 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.378 ± 0.032

6.917 ± 0.07

2.514 ± 0.039

2.962 ± 0.032

2.89 ± 0.041

6.496 ± 0.055

5.251 ± 0.054

6.442 ± 0.06

1.034 ± 0.023

4.045 ± 0.052
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski