Pseudocowpox virus

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes; Chitovirales; Poxviridae; Chordopoxvirinae; Parapoxvirus

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 125 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D3IZN4|D3IZN4_9POXV Major core protein 4a precursor OS=Pseudocowpox virus OX=129726 PE=3 SV=1
MM1 pKa = 7.47EE2 pKa = 4.7EE3 pKa = 4.33SVNVEE8 pKa = 3.99YY9 pKa = 10.95ADD11 pKa = 3.77EE12 pKa = 5.75DD13 pKa = 3.69EE14 pKa = 6.07DD15 pKa = 4.58EE16 pKa = 4.82IEE18 pKa = 4.16EE19 pKa = 4.24YY20 pKa = 10.73EE21 pKa = 4.15EE22 pKa = 4.16EE23 pKa = 4.21EE24 pKa = 4.23EE25 pKa = 4.39EE26 pKa = 4.27EE27 pKa = 4.36EE28 pKa = 4.26EE29 pKa = 4.35EE30 pKa = 4.42SAEE33 pKa = 4.1GAAGSSVSDD42 pKa = 3.54VAISATEE49 pKa = 3.74KK50 pKa = 10.67LVASEE55 pKa = 4.32VPEE58 pKa = 4.45DD59 pKa = 4.45AVEE62 pKa = 3.9TDD64 pKa = 3.18TNVRR68 pKa = 11.84QRR70 pKa = 11.84VTARR74 pKa = 11.84VEE76 pKa = 3.97EE77 pKa = 4.33LKK79 pKa = 10.9ARR81 pKa = 11.84YY82 pKa = 6.43TRR84 pKa = 11.84RR85 pKa = 11.84MSLFEE90 pKa = 3.78LTGIVAEE97 pKa = 4.73SFNLLCRR104 pKa = 11.84GRR106 pKa = 11.84LPLVADD112 pKa = 4.18AADD115 pKa = 3.79PALDD119 pKa = 3.88SEE121 pKa = 4.79LKK123 pKa = 9.91VVVRR127 pKa = 11.84EE128 pKa = 4.22LEE130 pKa = 4.08EE131 pKa = 4.58GVCPIVIEE139 pKa = 4.39KK140 pKa = 10.61NGEE143 pKa = 3.73FLAPGDD149 pKa = 4.6FDD151 pKa = 4.57PEE153 pKa = 4.12CLKK156 pKa = 11.07YY157 pKa = 10.34HH158 pKa = 6.82LNYY161 pKa = 7.97MTDD164 pKa = 2.86LWKK167 pKa = 10.99SQGRR171 pKa = 11.84MM172 pKa = 3.26

Molecular weight: 19.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D3IZC4|D3IZC4_9POXV Putative virion morphogenesis protein OS=Pseudocowpox virus OX=129726 GN=ORF107 PE=3 SV=1
MM1 pKa = 7.2IVAAFDD7 pKa = 3.71LGTRR11 pKa = 11.84NPARR15 pKa = 11.84TVLEE19 pKa = 4.08VLDD22 pKa = 3.68GRR24 pKa = 11.84VRR26 pKa = 11.84VVDD29 pKa = 3.7VAKK32 pKa = 10.72LDD34 pKa = 3.64WSRR37 pKa = 11.84DD38 pKa = 2.95WEE40 pKa = 4.37KK41 pKa = 10.59RR42 pKa = 11.84VHH44 pKa = 6.63RR45 pKa = 11.84DD46 pKa = 3.03VTAFPANVVLVEE58 pKa = 4.42RR59 pKa = 11.84QCKK62 pKa = 8.9MSPFSKK68 pKa = 10.14FIYY71 pKa = 9.95FIRR74 pKa = 11.84GLLYY78 pKa = 10.3DD79 pKa = 3.4GRR81 pKa = 11.84RR82 pKa = 11.84RR83 pKa = 11.84TRR85 pKa = 11.84VLAVPPAMTGSTYY98 pKa = 9.88RR99 pKa = 11.84QRR101 pKa = 11.84KK102 pKa = 6.96RR103 pKa = 11.84RR104 pKa = 11.84SVRR107 pKa = 11.84TFLALAEE114 pKa = 4.25SFGILEE120 pKa = 4.4AVPARR125 pKa = 11.84KK126 pKa = 9.81KK127 pKa = 10.89LDD129 pKa = 3.73DD130 pKa = 3.82VADD133 pKa = 4.02SFNMAINYY141 pKa = 7.48VLRR144 pKa = 11.84TNN146 pKa = 3.72

Molecular weight: 16.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

125

0

125

40573

53

1288

324.6

36.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.487 ± 0.341

2.312 ± 0.117

5.883 ± 0.131

6.142 ± 0.164

4.614 ± 0.17

5.548 ± 0.16

2.26 ± 0.1

3.939 ± 0.166

3.534 ± 0.199

9.558 ± 0.242

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.825 ± 0.112

3.419 ± 0.149

4.998 ± 0.198

2.053 ± 0.093

8.272 ± 0.21

6.615 ± 0.208

5.378 ± 0.151

8.592 ± 0.188

0.69 ± 0.057

2.881 ± 0.11
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski