Amino acid dipepetide frequency for Pseudocowpox virus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.208AlaAla: 15.208 ± 1.327
2.342AlaCys: 2.342 ± 0.296
6.975AlaAsp: 6.975 ± 0.511
6.285AlaGlu: 6.285 ± 0.578
3.697AlaPhe: 3.697 ± 0.367
5.866AlaGly: 5.866 ± 0.589
1.873AlaHis: 1.873 ± 0.196
3.599AlaIle: 3.599 ± 0.278
2.834AlaLys: 2.834 ± 0.249
10.574AlaLeu: 10.574 ± 0.822
2.218AlaMet: 2.218 ± 0.26
2.687AlaAsn: 2.687 ± 0.268
5.694AlaPro: 5.694 ± 0.503
2.144AlaGln: 2.144 ± 0.229
10.598AlaArg: 10.598 ± 0.724
5.792AlaSer: 5.792 ± 0.385
4.609AlaThr: 4.609 ± 0.352
9.194AlaVal: 9.194 ± 0.546
0.616AlaTrp: 0.616 ± 0.14
2.046AlaTyr: 2.046 ± 0.201
0.0AlaXaa: 0.0 ± 0.0
Cys
2.391CysAla: 2.391 ± 0.315
0.813CysCys: 0.813 ± 0.154
1.38CysAsp: 1.38 ± 0.195
1.356CysGlu: 1.356 ± 0.164
0.887CysPhe: 0.887 ± 0.141
1.701CysGly: 1.701 ± 0.197
0.32CysHis: 0.32 ± 0.078
0.887CysIle: 0.887 ± 0.173
0.838CysLys: 0.838 ± 0.139
2.366CysLeu: 2.366 ± 0.303
0.616CysMet: 0.616 ± 0.111
0.567CysAsn: 0.567 ± 0.116
1.06CysPro: 1.06 ± 0.28
0.345CysGln: 0.345 ± 0.106
1.725CysArg: 1.725 ± 0.19
1.454CysSer: 1.454 ± 0.205
1.158CysThr: 1.158 ± 0.16
2.391CysVal: 2.391 ± 0.284
0.222CysTrp: 0.222 ± 0.077
0.641CysTyr: 0.641 ± 0.129
0.0CysXaa: 0.0 ± 0.0
Asp
7.074AspAla: 7.074 ± 0.452
0.986AspCys: 0.986 ± 0.147
3.894AspAsp: 3.894 ± 0.437
4.141AspGlu: 4.141 ± 0.303
3.081AspPhe: 3.081 ± 0.239
4.042AspGly: 4.042 ± 0.351
0.789AspHis: 0.789 ± 0.164
2.514AspIle: 2.514 ± 0.276
1.873AspLys: 1.873 ± 0.33
5.102AspLeu: 5.102 ± 0.344
1.972AspMet: 1.972 ± 0.196
2.095AspAsn: 2.095 ± 0.267
3.081AspPro: 3.081 ± 0.298
0.739AspGln: 0.739 ± 0.147
3.401AspArg: 3.401 ± 0.303
4.018AspSer: 4.018 ± 0.424
2.563AspThr: 2.563 ± 0.306
5.521AspVal: 5.521 ± 0.415
0.296AspTrp: 0.296 ± 0.09
1.651AspTyr: 1.651 ± 0.206
0.0AspXaa: 0.0 ± 0.0
Glu
5.472GluAla: 5.472 ± 0.392
1.158GluCys: 1.158 ± 0.213
3.87GluAsp: 3.87 ± 0.339
4.954GluGlu: 4.954 ± 0.582
3.056GluPhe: 3.056 ± 0.274
2.785GluGly: 2.785 ± 0.285
1.405GluHis: 1.405 ± 0.183
2.761GluIle: 2.761 ± 0.285
2.637GluLys: 2.637 ± 0.307
5.496GluLeu: 5.496 ± 0.418
1.947GluMet: 1.947 ± 0.204
2.218GluAsn: 2.218 ± 0.256
2.687GluPro: 2.687 ± 0.307
1.405GluGln: 1.405 ± 0.194
5.225GluArg: 5.225 ± 0.453
3.401GluSer: 3.401 ± 0.319
3.82GluThr: 3.82 ± 0.293
4.461GluVal: 4.461 ± 0.34
0.665GluTrp: 0.665 ± 0.128
1.996GluTyr: 1.996 ± 0.226
0.0GluXaa: 0.0 ± 0.0
Phe
4.634PheAla: 4.634 ± 0.351
1.232PheCys: 1.232 ± 0.174
2.982PheAsp: 2.982 ± 0.249
2.563PheGlu: 2.563 ± 0.233
2.415PhePhe: 2.415 ± 0.222
2.588PheGly: 2.588 ± 0.247
1.06PheHis: 1.06 ± 0.17
1.306PheIle: 1.306 ± 0.232
1.725PheLys: 1.725 ± 0.252
4.412PheLeu: 4.412 ± 0.375
1.232PheMet: 1.232 ± 0.169
1.898PheAsn: 1.898 ± 0.188
2.046PhePro: 2.046 ± 0.219
0.739PheGln: 0.739 ± 0.135
3.007PheArg: 3.007 ± 0.283
3.672PheSer: 3.672 ± 0.277
2.366PheThr: 2.366 ± 0.286
4.634PheVal: 4.634 ± 0.36
0.419PheTrp: 0.419 ± 0.117
1.208PheTyr: 1.208 ± 0.194
0.0PheXaa: 0.0 ± 0.0
Gly
7.37GlyAla: 7.37 ± 0.635
1.282GlyCys: 1.282 ± 0.16
3.278GlyAsp: 3.278 ± 0.315
3.401GlyGlu: 3.401 ± 0.398
2.539GlyPhe: 2.539 ± 0.279
3.87GlyGly: 3.87 ± 0.384
1.109GlyHis: 1.109 ± 0.199
2.317GlyIle: 2.317 ± 0.261
2.021GlyLys: 2.021 ± 0.262
4.264GlyLeu: 4.264 ± 0.311
1.43GlyMet: 1.43 ± 0.187
1.873GlyAsn: 1.873 ± 0.186
1.824GlyPro: 1.824 ± 0.283
0.986GlyGln: 0.986 ± 0.163
4.856GlyArg: 4.856 ± 0.336
3.5GlySer: 3.5 ± 0.325
2.761GlyThr: 2.761 ± 0.274
4.856GlyVal: 4.856 ± 0.387
0.493GlyTrp: 0.493 ± 0.104
1.454GlyTyr: 1.454 ± 0.168
0.0GlyXaa: 0.0 ± 0.0
His
1.947HisAla: 1.947 ± 0.218
0.468HisCys: 0.468 ± 0.12
1.158HisAsp: 1.158 ± 0.15
0.912HisGlu: 0.912 ± 0.153
0.986HisPhe: 0.986 ± 0.144
1.109HisGly: 1.109 ± 0.193
0.641HisHis: 0.641 ± 0.131
1.306HisIle: 1.306 ± 0.157
0.665HisLys: 0.665 ± 0.139
2.366HisLeu: 2.366 ± 0.224
0.764HisMet: 0.764 ± 0.125
0.863HisAsn: 0.863 ± 0.157
1.257HisPro: 1.257 ± 0.18
0.345HisGln: 0.345 ± 0.101
1.972HisArg: 1.972 ± 0.237
1.405HisSer: 1.405 ± 0.195
1.405HisThr: 1.405 ± 0.19
2.243HisVal: 2.243 ± 0.29
0.222HisTrp: 0.222 ± 0.07
0.567HisTyr: 0.567 ± 0.104
0.0HisXaa: 0.0 ± 0.0
Ile
3.919IleAla: 3.919 ± 0.301
1.183IleCys: 1.183 ± 0.167
2.07IleAsp: 2.07 ± 0.211
2.736IleGlu: 2.736 ± 0.253
2.268IlePhe: 2.268 ± 0.231
1.528IleGly: 1.528 ± 0.237
0.813IleHis: 0.813 ± 0.15
1.479IleIle: 1.479 ± 0.24
1.553IleLys: 1.553 ± 0.241
3.106IleLeu: 3.106 ± 0.384
1.109IleMet: 1.109 ± 0.18
2.046IleAsn: 2.046 ± 0.253
2.046IlePro: 2.046 ± 0.259
0.863IleGln: 0.863 ± 0.164
2.908IleArg: 2.908 ± 0.284
2.81IleSer: 2.81 ± 0.365
1.824IleThr: 1.824 ± 0.196
3.648IleVal: 3.648 ± 0.381
0.173IleTrp: 0.173 ± 0.058
1.306IleTyr: 1.306 ± 0.188
0.0IleXaa: 0.0 ± 0.0
Lys
2.144LysAla: 2.144 ± 0.239
0.665LysCys: 0.665 ± 0.156
2.046LysAsp: 2.046 ± 0.252
2.021LysGlu: 2.021 ± 0.248
1.627LysPhe: 1.627 ± 0.322
1.306LysGly: 1.306 ± 0.156
0.863LysHis: 0.863 ± 0.138
2.317LysIle: 2.317 ± 0.356
2.07LysLys: 2.07 ± 0.252
3.82LysLeu: 3.82 ± 0.317
0.986LysMet: 0.986 ± 0.166
1.676LysAsn: 1.676 ± 0.235
1.676LysPro: 1.676 ± 0.248
0.887LysGln: 0.887 ± 0.158
2.489LysArg: 2.489 ± 0.276
2.218LysSer: 2.218 ± 0.191
2.662LysThr: 2.662 ± 0.245
2.317LysVal: 2.317 ± 0.247
0.099LysTrp: 0.099 ± 0.063
1.454LysTyr: 1.454 ± 0.205
0.0LysXaa: 0.0 ± 0.0
Leu
10.105LeuAla: 10.105 ± 0.587
2.613LeuCys: 2.613 ± 0.262
5.447LeuAsp: 5.447 ± 0.48
5.201LeuGlu: 5.201 ± 0.362
4.806LeuPhe: 4.806 ± 0.358
5.077LeuGly: 5.077 ± 0.347
2.243LeuHis: 2.243 ± 0.298
3.549LeuIle: 3.549 ± 0.334
3.697LeuLys: 3.697 ± 0.325
9.785LeuLeu: 9.785 ± 0.627
3.204LeuMet: 3.204 ± 0.332
3.18LeuAsn: 3.18 ± 0.345
3.87LeuPro: 3.87 ± 0.343
1.898LeuGln: 1.898 ± 0.238
8.306LeuArg: 8.306 ± 0.525
6.334LeuSer: 6.334 ± 0.369
5.077LeuThr: 5.077 ± 0.359
8.06LeuVal: 8.06 ± 0.493
0.444LeuTrp: 0.444 ± 0.118
2.687LeuTyr: 2.687 ± 0.272
0.0LeuXaa: 0.0 ± 0.0
Met
2.711MetAla: 2.711 ± 0.255
0.444MetCys: 0.444 ± 0.094
1.799MetAsp: 1.799 ± 0.224
2.194MetGlu: 2.194 ± 0.25
1.257MetPhe: 1.257 ± 0.184
1.405MetGly: 1.405 ± 0.224
0.641MetHis: 0.641 ± 0.116
1.134MetIle: 1.134 ± 0.164
1.011MetLys: 1.011 ± 0.175
2.662MetLeu: 2.662 ± 0.228
0.444MetMet: 0.444 ± 0.102
0.813MetAsn: 0.813 ± 0.214
1.282MetPro: 1.282 ± 0.162
0.739MetGln: 0.739 ± 0.133
2.711MetArg: 2.711 ± 0.256
1.873MetSer: 1.873 ± 0.258
1.676MetThr: 1.676 ± 0.235
1.898MetVal: 1.898 ± 0.243
0.222MetTrp: 0.222 ± 0.067
1.331MetTyr: 1.331 ± 0.175
0.0MetXaa: 0.0 ± 0.0
Asn
2.958AsnAla: 2.958 ± 0.34
0.542AsnCys: 0.542 ± 0.118
1.503AsnAsp: 1.503 ± 0.191
1.701AsnGlu: 1.701 ± 0.222
1.873AsnPhe: 1.873 ± 0.223
2.12AsnGly: 2.12 ± 0.232
0.789AsnHis: 0.789 ± 0.139
1.824AsnIle: 1.824 ± 0.239
1.282AsnLys: 1.282 ± 0.193
2.662AsnLeu: 2.662 ± 0.292
1.331AsnMet: 1.331 ± 0.211
1.824AsnAsn: 1.824 ± 0.282
1.824AsnPro: 1.824 ± 0.219
0.69AsnGln: 0.69 ± 0.137
2.07AsnArg: 2.07 ± 0.222
1.972AsnSer: 1.972 ± 0.236
2.268AsnThr: 2.268 ± 0.308
3.327AsnVal: 3.327 ± 0.295
0.123AsnTrp: 0.123 ± 0.051
1.503AsnTyr: 1.503 ± 0.225
0.0AsnXaa: 0.0 ± 0.0
Pro
5.768ProAla: 5.768 ± 0.594
1.158ProCys: 1.158 ± 0.164
3.18ProAsp: 3.18 ± 0.304
3.968ProGlu: 3.968 ± 0.346
1.676ProPhe: 1.676 ± 0.225
3.204ProGly: 3.204 ± 0.33
1.232ProHis: 1.232 ± 0.155
1.331ProIle: 1.331 ± 0.193
1.232ProLys: 1.232 ± 0.218
4.782ProLeu: 4.782 ± 0.412
1.109ProMet: 1.109 ± 0.175
1.232ProAsn: 1.232 ± 0.189
3.845ProPro: 3.845 ± 0.491
1.158ProGln: 1.158 ± 0.151
3.648ProArg: 3.648 ± 0.399
3.451ProSer: 3.451 ± 0.304
2.908ProThr: 2.908 ± 0.333
3.623ProVal: 3.623 ± 0.25
0.468ProTrp: 0.468 ± 0.118
1.011ProTyr: 1.011 ± 0.157
0.0ProXaa: 0.0 ± 0.0
Gln
1.43GlnAla: 1.43 ± 0.207
0.37GlnCys: 0.37 ± 0.099
1.011GlnAsp: 1.011 ± 0.157
1.282GlnGlu: 1.282 ± 0.228
0.937GlnPhe: 0.937 ± 0.157
0.887GlnGly: 0.887 ± 0.142
0.739GlnHis: 0.739 ± 0.146
0.986GlnIle: 0.986 ± 0.15
0.863GlnLys: 0.863 ± 0.16
2.144GlnLeu: 2.144 ± 0.263
0.715GlnMet: 0.715 ± 0.141
0.69GlnAsn: 0.69 ± 0.139
1.06GlnPro: 1.06 ± 0.207
0.493GlnGln: 0.493 ± 0.11
1.873GlnArg: 1.873 ± 0.215
1.479GlnSer: 1.479 ± 0.217
1.503GlnThr: 1.503 ± 0.226
1.331GlnVal: 1.331 ± 0.192
0.222GlnTrp: 0.222 ± 0.061
0.518GlnTyr: 0.518 ± 0.112
0.0GlnXaa: 0.0 ± 0.0
Arg
9.317ArgAla: 9.317 ± 0.67
1.873ArgCys: 1.873 ± 0.23
3.845ArgAsp: 3.845 ± 0.306
5.127ArgGlu: 5.127 ± 0.372
3.697ArgPhe: 3.697 ± 0.308
4.338ArgGly: 4.338 ± 0.379
2.687ArgHis: 2.687 ± 0.278
2.761ArgIle: 2.761 ± 0.221
2.637ArgLys: 2.637 ± 0.264
8.084ArgLeu: 8.084 ± 0.414
2.514ArgMet: 2.514 ± 0.237
2.415ArgAsn: 2.415 ± 0.277
3.771ArgPro: 3.771 ± 0.34
2.144ArgGln: 2.144 ± 0.214
8.38ArgArg: 8.38 ± 0.797
5.176ArgSer: 5.176 ± 0.466
4.141ArgThr: 4.141 ± 0.317
7.172ArgVal: 7.172 ± 0.478
0.419ArgTrp: 0.419 ± 0.124
2.218ArgTyr: 2.218 ± 0.245
0.0ArgXaa: 0.0 ± 0.0
Ser
6.778SerAla: 6.778 ± 0.42
1.479SerCys: 1.479 ± 0.187
3.401SerAsp: 3.401 ± 0.37
3.574SerGlu: 3.574 ± 0.336
2.563SerPhe: 2.563 ± 0.238
4.165SerGly: 4.165 ± 0.416
1.306SerHis: 1.306 ± 0.167
2.859SerIle: 2.859 ± 0.322
2.539SerLys: 2.539 ± 0.27
6.26SerLeu: 6.26 ± 0.432
2.095SerMet: 2.095 ± 0.206
1.602SerAsn: 1.602 ± 0.227
3.574SerPro: 3.574 ± 0.35
1.454SerGln: 1.454 ± 0.195
5.324SerArg: 5.324 ± 0.511
5.398SerSer: 5.398 ± 0.755
3.82SerThr: 3.82 ± 0.441
5.891SerVal: 5.891 ± 0.346
0.419SerTrp: 0.419 ± 0.094
1.651SerTyr: 1.651 ± 0.192
0.0SerXaa: 0.0 ± 0.0
Thr
4.831ThrAla: 4.831 ± 0.358
1.011ThrCys: 1.011 ± 0.171
3.056ThrAsp: 3.056 ± 0.284
3.278ThrGlu: 3.278 ± 0.34
2.268ThrPhe: 2.268 ± 0.241
2.958ThrGly: 2.958 ± 0.328
1.528ThrHis: 1.528 ± 0.191
1.799ThrIle: 1.799 ± 0.263
2.021ThrLys: 2.021 ± 0.262
4.88ThrLeu: 4.88 ± 0.323
1.158ThrMet: 1.158 ± 0.186
1.898ThrAsn: 1.898 ± 0.215
4.264ThrPro: 4.264 ± 0.447
1.232ThrGln: 1.232 ± 0.199
4.363ThrArg: 4.363 ± 0.329
3.796ThrSer: 3.796 ± 0.389
2.711ThrThr: 2.711 ± 0.368
4.831ThrVal: 4.831 ± 0.331
0.37ThrTrp: 0.37 ± 0.097
1.528ThrTyr: 1.528 ± 0.249
0.0ThrXaa: 0.0 ± 0.0
Val
7.567ValAla: 7.567 ± 0.456
2.637ValCys: 2.637 ± 0.276
5.965ValAsp: 5.965 ± 0.403
4.93ValGlu: 4.93 ± 0.379
4.165ValPhe: 4.165 ± 0.385
4.141ValGly: 4.141 ± 0.326
1.873ValHis: 1.873 ± 0.234
2.736ValIle: 2.736 ± 0.309
2.81ValLys: 2.81 ± 0.327
9.292ValLeu: 9.292 ± 0.483
2.046ValMet: 2.046 ± 0.244
3.155ValAsn: 3.155 ± 0.335
3.574ValPro: 3.574 ± 0.298
1.701ValGln: 1.701 ± 0.19
7.419ValArg: 7.419 ± 0.379
6.285ValSer: 6.285 ± 0.42
4.634ValThr: 4.634 ± 0.358
7.419ValVal: 7.419 ± 0.494
0.69ValTrp: 0.69 ± 0.167
2.884ValTyr: 2.884 ± 0.31
0.0ValXaa: 0.0 ± 0.0
Trp
0.592TrpAla: 0.592 ± 0.114
0.123TrpCys: 0.123 ± 0.051
0.246TrpAsp: 0.246 ± 0.098
0.296TrpGlu: 0.296 ± 0.087
0.542TrpPhe: 0.542 ± 0.122
0.493TrpGly: 0.493 ± 0.131
0.123TrpHis: 0.123 ± 0.055
0.32TrpIle: 0.32 ± 0.114
0.173TrpLys: 0.173 ± 0.065
0.69TrpLeu: 0.69 ± 0.148
0.32TrpMet: 0.32 ± 0.092
0.148TrpAsn: 0.148 ± 0.067
0.419TrpPro: 0.419 ± 0.104
0.074TrpGln: 0.074 ± 0.041
0.715TrpArg: 0.715 ± 0.121
0.37TrpSer: 0.37 ± 0.102
0.567TrpThr: 0.567 ± 0.132
0.296TrpVal: 0.296 ± 0.091
0.123TrpTrp: 0.123 ± 0.094
0.271TrpTyr: 0.271 ± 0.078
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.662TyrAla: 2.662 ± 0.283
0.739TyrCys: 0.739 ± 0.124
1.725TyrAsp: 1.725 ± 0.22
1.503TyrGlu: 1.503 ± 0.192
1.799TyrPhe: 1.799 ± 0.236
1.898TyrGly: 1.898 ± 0.214
0.567TyrHis: 0.567 ± 0.116
1.602TyrIle: 1.602 ± 0.236
0.863TyrLys: 0.863 ± 0.199
2.834TyrLeu: 2.834 ± 0.238
1.011TyrMet: 1.011 ± 0.155
1.306TyrAsn: 1.306 ± 0.208
1.134TyrPro: 1.134 ± 0.169
0.493TyrGln: 0.493 ± 0.11
1.725TyrArg: 1.725 ± 0.209
1.75TyrSer: 1.75 ± 0.236
1.306TyrThr: 1.306 ± 0.2
2.81TyrVal: 2.81 ± 0.241
0.197TyrTrp: 0.197 ± 0.058
0.887TyrTyr: 0.887 ± 0.151
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 125 proteins (40573 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski