Kozakia baliensis
Average proteome isoelectric point is 6.76
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3115 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1D8UV41|A0A1D8UV41_9PROT CAAX protease OS=Kozakia baliensis OX=153496 GN=A0U89_10575 PE=4 SV=1
MM1 pKa = 7.22 ATYY4 pKa = 7.62 PTLDD8 pKa = 3.52 SLYY11 pKa = 10.74 GAIKK15 pKa = 10.57 NGSLNLSDD23 pKa = 5.17 GLPTYY28 pKa = 10.86 GGDD31 pKa = 3.82 EE32 pKa = 5.11 PIDD35 pKa = 3.95 APEE38 pKa = 3.7 IWSWDD43 pKa = 3.47 PYY45 pKa = 10.69 RR46 pKa = 11.84 YY47 pKa = 8.78 MVGTSAADD55 pKa = 3.35 LALIPRR61 pKa = 11.84 DD62 pKa = 3.52 DD63 pKa = 3.69 WEE65 pKa = 5.51 GEE67 pKa = 4.45 VKK69 pKa = 10.72 FEE71 pKa = 4.2 EE72 pKa = 4.52
Molecular weight: 7.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.759
IPC2_protein 3.77
IPC_protein 3.694
Toseland 3.49
ProMoST 3.834
Dawson 3.694
Bjellqvist 3.935
Wikipedia 3.643
Rodwell 3.528
Grimsley 3.414
Solomon 3.656
Lehninger 3.617
Nozaki 3.834
DTASelect 4.024
Thurlkill 3.567
EMBOSS 3.656
Sillero 3.821
Patrickios 1.863
IPC_peptide 3.656
IPC2_peptide 3.783
IPC2.peptide.svr19 3.769
Protein with the highest isoelectric point:
>tr|A0A1D8UQZ0|A0A1D8UQZ0_9PROT Aminotransferase OS=Kozakia baliensis OX=153496 GN=hisC PE=4 SV=1
MM1 pKa = 6.42 VTWTGIARR9 pKa = 11.84 RR10 pKa = 11.84 EE11 pKa = 3.94 YY12 pKa = 9.35 SRR14 pKa = 11.84 EE15 pKa = 3.62 RR16 pKa = 11.84 LRR18 pKa = 11.84 YY19 pKa = 8.7 PSDD22 pKa = 3.24 MTDD25 pKa = 3.97 GEE27 pKa = 4.51 WTLIMPFVPPAKK39 pKa = 9.91 RR40 pKa = 11.84 GGRR43 pKa = 11.84 PRR45 pKa = 11.84 TTDD48 pKa = 2.64 MRR50 pKa = 11.84 EE51 pKa = 3.88 VVNAMLYY58 pKa = 9.87 IASAGCAWRR67 pKa = 11.84 LLPKK71 pKa = 10.22 CFPPVSTIRR80 pKa = 11.84 RR81 pKa = 11.84 YY82 pKa = 9.62 FYY84 pKa = 9.98 AWRR87 pKa = 11.84 DD88 pKa = 3.32 AGVFEE93 pKa = 4.82 VMNTVLVMSLRR104 pKa = 11.84 EE105 pKa = 3.91 IEE107 pKa = 4.67 GRR109 pKa = 11.84 DD110 pKa = 3.52 ASPSAGVIDD119 pKa = 4.01 SQSVKK124 pKa = 7.24 TTEE127 pKa = 3.84 SGGISGYY134 pKa = 10.61 DD135 pKa = 2.87 AGKK138 pKa = 9.88 KK139 pKa = 8.46 VKK141 pKa = 9.6 GRR143 pKa = 11.84 KK144 pKa = 8.03 RR145 pKa = 11.84 HH146 pKa = 5.78 IVTDD150 pKa = 3.41 TCGFLIFLLVHH161 pKa = 6.58 AADD164 pKa = 3.48 IQDD167 pKa = 3.23 RR168 pKa = 11.84 DD169 pKa = 3.66 GAVDD173 pKa = 3.16 VLAAIRR179 pKa = 11.84 RR180 pKa = 11.84 RR181 pKa = 11.84 FPWLRR186 pKa = 11.84 HH187 pKa = 4.76 IFADD191 pKa = 4.38 GGYY194 pKa = 10.41 AGDD197 pKa = 4.06 KK198 pKa = 10.31 LRR200 pKa = 11.84 SALASMGKK208 pKa = 6.85 WTLEE212 pKa = 3.63 IIRR215 pKa = 11.84 RR216 pKa = 11.84 SDD218 pKa = 3.34 TVKK221 pKa = 10.55 GFQILPRR228 pKa = 11.84 RR229 pKa = 11.84 WVVEE233 pKa = 3.94 RR234 pKa = 11.84 TFAWLRR240 pKa = 11.84 RR241 pKa = 11.84 CRR243 pKa = 11.84 RR244 pKa = 11.84 LAKK247 pKa = 10.36 DD248 pKa = 3.31 WEE250 pKa = 4.07 QSIASSTAWTLIASIRR266 pKa = 11.84 MLTRR270 pKa = 11.84 RR271 pKa = 11.84 TARR274 pKa = 11.84 HH275 pKa = 5.25 CQGG278 pKa = 3.22
Molecular weight: 31.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.351
IPC2_protein 9.575
IPC_protein 10.452
Toseland 10.584
ProMoST 10.335
Dawson 10.701
Bjellqvist 10.438
Wikipedia 10.935
Rodwell 10.818
Grimsley 10.76
Solomon 10.818
Lehninger 10.789
Nozaki 10.584
DTASelect 10.423
Thurlkill 10.599
EMBOSS 10.994
Sillero 10.628
Patrickios 10.496
IPC_peptide 10.818
IPC2_peptide 9.589
IPC2.peptide.svr19 8.599
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3115
0
3115
985054
26
1709
316.2
34.58
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.511 ± 0.05
0.951 ± 0.013
5.462 ± 0.033
5.462 ± 0.044
3.616 ± 0.027
8.091 ± 0.038
2.527 ± 0.019
5.345 ± 0.03
3.132 ± 0.032
10.463 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.467 ± 0.019
2.96 ± 0.033
5.39 ± 0.033
3.643 ± 0.028
7.245 ± 0.05
5.823 ± 0.035
5.422 ± 0.032
6.8 ± 0.035
1.423 ± 0.02
2.265 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here