Victorian trout aquabirnavirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Birnaviridae; Aquabirnavirus; unclassified Aquabirnavirus

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N9H8A4|A0A0N9H8A4_9VIRU Protein VP5 OS=Victorian trout aquabirnavirus OX=1661392 PE=3 SV=1
MM1 pKa = 6.89NTNKK5 pKa = 8.97ATATYY10 pKa = 9.42LRR12 pKa = 11.84SIMLPEE18 pKa = 4.78NGPASIPDD26 pKa = 5.04DD27 pKa = 3.12ITEE30 pKa = 3.99RR31 pKa = 11.84HH32 pKa = 5.89ILKK35 pKa = 10.07QEE37 pKa = 3.84TSSYY41 pKa = 10.0NLEE44 pKa = 4.15VSDD47 pKa = 4.41SGSGLLVCFPGAPGSRR63 pKa = 11.84IGAHH67 pKa = 5.46YY68 pKa = 10.21RR69 pKa = 11.84WNQNQTALEE78 pKa = 3.92FDD80 pKa = 3.2QWLEE84 pKa = 3.86TSQDD88 pKa = 3.07LKK90 pKa = 11.21KK91 pKa = 10.75AFNYY95 pKa = 9.81GRR97 pKa = 11.84LISRR101 pKa = 11.84KK102 pKa = 9.31YY103 pKa = 10.36DD104 pKa = 3.38IQSSTLPAGLYY115 pKa = 10.34ALNGTLNAATFEE127 pKa = 4.46GSLSEE132 pKa = 4.52VEE134 pKa = 4.03NFSYY138 pKa = 11.02NSLMSLTTNPQDD150 pKa = 3.31KK151 pKa = 10.9VNNQLVTKK159 pKa = 10.21GVTVLNLPIGFDD171 pKa = 3.18KK172 pKa = 10.88PYY174 pKa = 10.86VRR176 pKa = 11.84LEE178 pKa = 4.3DD179 pKa = 3.78EE180 pKa = 4.8TPQGPQSMNGAKK192 pKa = 8.85MRR194 pKa = 11.84CTAAVAPRR202 pKa = 11.84RR203 pKa = 11.84YY204 pKa = 10.13EE205 pKa = 3.5IDD207 pKa = 3.65LPTEE211 pKa = 3.97RR212 pKa = 11.84LPPVLAIGTLQTIYY226 pKa = 10.51EE227 pKa = 4.31GNADD231 pKa = 3.56IVNSTTVTGDD241 pKa = 3.0INFQLAANPANDD253 pKa = 3.61TKK255 pKa = 11.09FDD257 pKa = 3.79FQLDD261 pKa = 3.79FLGMDD266 pKa = 3.46NNDD269 pKa = 3.36PVVSVTSSVLATADD283 pKa = 3.12NYY285 pKa = 11.07RR286 pKa = 11.84GVSVKK291 pKa = 8.86FTQSIPTEE299 pKa = 4.42SVTKK303 pKa = 10.21PITRR307 pKa = 11.84VRR309 pKa = 11.84LSYY312 pKa = 10.46KK313 pKa = 10.06INQQTSIDD321 pKa = 3.64NAATLGAMGPASVSFSSGNGNVPGVLRR348 pKa = 11.84PVTLVAYY355 pKa = 9.85EE356 pKa = 4.1KK357 pKa = 7.76MTPQSILTVAGVSNYY372 pKa = 9.4EE373 pKa = 4.44LIPNPEE379 pKa = 4.27LLKK382 pKa = 11.42NMVTRR387 pKa = 11.84YY388 pKa = 9.74GKK390 pKa = 10.45YY391 pKa = 10.22DD392 pKa = 3.71PEE394 pKa = 4.29GLNYY398 pKa = 10.95AKK400 pKa = 10.06MILSHH405 pKa = 7.46RR406 pKa = 11.84EE407 pKa = 3.56EE408 pKa = 4.78LDD410 pKa = 2.89IRR412 pKa = 11.84TVWRR416 pKa = 11.84TEE418 pKa = 3.79EE419 pKa = 3.97YY420 pKa = 10.52KK421 pKa = 10.9EE422 pKa = 3.66RR423 pKa = 11.84TRR425 pKa = 11.84AFNEE429 pKa = 3.69ITDD432 pKa = 4.43FSSDD436 pKa = 3.78LPTSKK441 pKa = 10.5AWGWRR446 pKa = 11.84DD447 pKa = 2.97IVRR450 pKa = 11.84GIRR453 pKa = 11.84KK454 pKa = 8.54VAAPVLSTIFPMAAPLIGAADD475 pKa = 3.69QFIGDD480 pKa = 3.94LTKK483 pKa = 10.75TNAAGGRR490 pKa = 11.84YY491 pKa = 8.8HH492 pKa = 6.58SHH494 pKa = 6.69AAGGRR499 pKa = 11.84HH500 pKa = 5.94RR501 pKa = 11.84DD502 pKa = 3.75VMDD505 pKa = 3.07SWTSGAQTGKK515 pKa = 10.67FSLKK519 pKa = 10.18LKK521 pKa = 10.61NRR523 pKa = 11.84LEE525 pKa = 4.32SNNYY529 pKa = 9.42EE530 pKa = 4.5EE531 pKa = 5.88IEE533 pKa = 4.44LPPPTKK539 pKa = 10.4GVIIPVVHH547 pKa = 5.75TVEE550 pKa = 4.38SAPGEE555 pKa = 4.18AFGSLLIVIPGEE567 pKa = 4.15YY568 pKa = 9.86PEE570 pKa = 5.73LLDD573 pKa = 4.94PNQQVLSHH581 pKa = 6.63FEE583 pKa = 3.92NDD585 pKa = 3.24TGGVWGIGEE594 pKa = 5.23DD595 pKa = 3.74IPFEE599 pKa = 5.09GDD601 pKa = 3.23DD602 pKa = 3.73TVYY605 pKa = 10.19TALPLKK611 pKa = 9.88EE612 pKa = 3.84IKK614 pKa = 10.48KK615 pKa = 9.85NGNIVVEE622 pKa = 4.79KK623 pKa = 10.31ILAGPAMGPSCQLGLSLLVNDD644 pKa = 4.51IEE646 pKa = 4.51EE647 pKa = 4.54GVPRR651 pKa = 11.84MVFTGEE657 pKa = 3.48ISGDD661 pKa = 3.69EE662 pKa = 3.94EE663 pKa = 4.68TIIPICGVDD672 pKa = 3.33IKK674 pKa = 11.15AIAAHH679 pKa = 5.37EE680 pKa = 4.6HH681 pKa = 5.85GLPLVGCQPGVDD693 pKa = 2.91EE694 pKa = 4.85VVANTSLASHH704 pKa = 7.65LIQTGALPVQKK715 pKa = 8.87TQGASRR721 pKa = 11.84RR722 pKa = 11.84IRR724 pKa = 11.84YY725 pKa = 9.13LGEE728 pKa = 3.91LMRR731 pKa = 11.84TTASGMDD738 pKa = 3.75EE739 pKa = 4.16EE740 pKa = 5.06LQGLLQATMARR751 pKa = 11.84AKK753 pKa = 9.64EE754 pKa = 4.16VKK756 pKa = 10.05DD757 pKa = 3.58VEE759 pKa = 4.21IFKK762 pKa = 10.63LLKK765 pKa = 10.5LMSWTRR771 pKa = 11.84KK772 pKa = 9.6NDD774 pKa = 3.21LTDD777 pKa = 4.97NLFEE781 pKa = 4.49WSRR784 pKa = 11.84EE785 pKa = 3.88DD786 pKa = 3.31PDD788 pKa = 4.48AIKK791 pKa = 10.39FGRR794 pKa = 11.84LISTPPKK801 pKa = 9.86HH802 pKa = 5.66QEE804 pKa = 3.87KK805 pKa = 10.39PKK807 pKa = 11.16GPDD810 pKa = 2.69QHH812 pKa = 6.27TAQEE816 pKa = 4.05AKK818 pKa = 10.22AVRR821 pKa = 11.84ISLDD825 pKa = 3.19AVRR828 pKa = 11.84AGADD832 pKa = 3.43FASPEE837 pKa = 3.89WVADD841 pKa = 3.39NGYY844 pKa = 10.12RR845 pKa = 11.84GPNPGQFKK853 pKa = 10.56YY854 pKa = 11.48YY855 pKa = 9.28MITGEE860 pKa = 4.32VPNPGDD866 pKa = 3.8DD867 pKa = 3.71YY868 pKa = 11.34EE869 pKa = 5.92DD870 pKa = 3.89YY871 pKa = 10.92VRR873 pKa = 11.84KK874 pKa = 9.52PITRR878 pKa = 11.84PTNMDD883 pKa = 3.82KK884 pKa = 10.37IRR886 pKa = 11.84RR887 pKa = 11.84LANSIYY893 pKa = 10.03GLPHH897 pKa = 5.99QEE899 pKa = 3.76PAPEE903 pKa = 3.88QFYY906 pKa = 11.03DD907 pKa = 3.48AVVEE911 pKa = 4.22VFATNSGRR919 pKa = 11.84GPDD922 pKa = 3.59QDD924 pKa = 3.46QMQDD928 pKa = 3.21LRR930 pKa = 11.84DD931 pKa = 3.54LARR934 pKa = 11.84QMKK937 pKa = 9.54RR938 pKa = 11.84RR939 pKa = 11.84PRR941 pKa = 11.84TAEE944 pKa = 3.58TRR946 pKa = 11.84RR947 pKa = 11.84QNQAPPRR954 pKa = 11.84AAPSGGSRR962 pKa = 11.84FTPSGNDD969 pKa = 3.65GEE971 pKa = 4.68VV972 pKa = 2.76

Molecular weight: 106.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0N9H8A4|A0A0N9H8A4_9VIRU Protein VP5 OS=Victorian trout aquabirnavirus OX=1661392 PE=3 SV=1
MM1 pKa = 6.92NHH3 pKa = 5.59EE4 pKa = 4.65HH5 pKa = 6.17KK6 pKa = 10.65QSNRR10 pKa = 11.84NLLEE14 pKa = 3.18IHH16 pKa = 5.83YY17 pKa = 10.31ASRR20 pKa = 11.84EE21 pKa = 3.97WSSKK25 pKa = 9.94HH26 pKa = 5.41SGRR29 pKa = 11.84HH30 pKa = 4.45NRR32 pKa = 11.84EE33 pKa = 3.49THH35 pKa = 6.06LKK37 pKa = 8.81TRR39 pKa = 11.84DD40 pKa = 3.42LVIQPRR46 pKa = 11.84GLRR49 pKa = 11.84LRR51 pKa = 11.84KK52 pKa = 8.61WASCLLPWCSWLKK65 pKa = 10.15NWCSLQVEE73 pKa = 4.6SEE75 pKa = 4.09PDD77 pKa = 3.25GSGIRR82 pKa = 11.84PVAGDD87 pKa = 3.22IPGPEE92 pKa = 4.01EE93 pKa = 4.42SIQLRR98 pKa = 11.84EE99 pKa = 3.85ADD101 pKa = 3.13ISEE104 pKa = 3.73IRR106 pKa = 11.84HH107 pKa = 5.53PEE109 pKa = 4.41FYY111 pKa = 10.65ASSWSVCAQRR121 pKa = 11.84DD122 pKa = 3.78PEE124 pKa = 4.17RR125 pKa = 11.84SHH127 pKa = 6.35VRR129 pKa = 11.84RR130 pKa = 11.84KK131 pKa = 10.05SVV133 pKa = 2.58

Molecular weight: 15.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1949

133

972

649.7

72.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.748 ± 0.611

0.616 ± 0.447

5.9 ± 0.417

6.516 ± 0.467

2.771 ± 0.38

6.773 ± 0.804

1.591 ± 0.83

5.028 ± 0.374

5.695 ± 0.814

9.082 ± 0.57

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.514 ± 0.414

5.336 ± 0.294

6.927 ± 0.17

3.848 ± 0.18

5.644 ± 1.068

6.721 ± 1.134

7.029 ± 1.049

5.644 ± 0.563

1.539 ± 0.594

3.079 ± 0.301
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski