Salipiger sp. IMCC34102
Average proteome isoelectric point is 6.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3341 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Q3Z7T4|A0A4Q3Z7T4_9RHOB Leucyl aminopeptidase family protein OS=Salipiger sp. IMCC34102 OX=2510647 GN=EU805_00405 PE=3 SV=1
MM1 pKa = 7.42 NKK3 pKa = 9.63 FLAIATASVVASAAQAQEE21 pKa = 4.15 TVVWWDD27 pKa = 3.94 FLAGGDD33 pKa = 4.04 GVRR36 pKa = 11.84 MKK38 pKa = 11.15 QMIEE42 pKa = 4.21 DD43 pKa = 4.05 FNAAHH48 pKa = 6.82 EE49 pKa = 4.48 GEE51 pKa = 4.26 IVIDD55 pKa = 3.63 ATTLEE60 pKa = 4.33 WGVPYY65 pKa = 8.75 YY66 pKa = 10.73 TKK68 pKa = 10.58 VQTSVAVGEE77 pKa = 4.86 APDD80 pKa = 2.99 IMTYY84 pKa = 9.79 HH85 pKa = 7.42 SSRR88 pKa = 11.84 IPLAIDD94 pKa = 2.99 QGLLQEE100 pKa = 4.58 LTPADD105 pKa = 4.5 FEE107 pKa = 4.92 TMGLGEE113 pKa = 5.67 DD114 pKa = 4.59 DD115 pKa = 4.45 FAPSIWDD122 pKa = 3.39 AVSADD127 pKa = 3.19 GAQYY131 pKa = 11.34 GMPLDD136 pKa = 3.43 THH138 pKa = 6.95 PIVLYY143 pKa = 9.49 YY144 pKa = 10.97 NKK146 pKa = 10.27 DD147 pKa = 3.35 ALEE150 pKa = 4.07 EE151 pKa = 4.13 AGRR154 pKa = 11.84 LTEE157 pKa = 5.66 DD158 pKa = 4.09 GLPMGLDD165 pKa = 3.17 SRR167 pKa = 11.84 EE168 pKa = 4.22 GFTQTLQALQDD179 pKa = 3.72 SGSVNFPLASVTADD193 pKa = 3.09 GNFMFRR199 pKa = 11.84 TIYY202 pKa = 11.0 SLMGQQGGEE211 pKa = 4.07 LMTDD215 pKa = 3.82 GEE217 pKa = 5.01 FLAGDD222 pKa = 3.67 NPQKK226 pKa = 10.87 LEE228 pKa = 3.85 NALTVLQEE236 pKa = 3.61 WTNAGFQSTYY246 pKa = 8.47 TDD248 pKa = 3.59 YY249 pKa = 10.57 PATVALFTNGEE260 pKa = 4.13 AGMMINGVWEE270 pKa = 4.56 VPTMIDD276 pKa = 5.07 LEE278 pKa = 4.55 AEE280 pKa = 4.13 GNLFNWGAVEE290 pKa = 4.27 LPVIFDD296 pKa = 3.57 QPATYY301 pKa = 10.52 SDD303 pKa = 3.5 SHH305 pKa = 7.55 VFAIPAGNDD314 pKa = 2.86 MSEE317 pKa = 4.37 EE318 pKa = 3.59 KK319 pKa = 10.26 RR320 pKa = 11.84 AAVLEE325 pKa = 4.3 VMSWFSNNSLFWATAGHH342 pKa = 6.55 IPANNSVQGTDD353 pKa = 3.58 EE354 pKa = 4.2 YY355 pKa = 11.62 QAMEE359 pKa = 4.35 PNATYY364 pKa = 10.99 SSLTEE369 pKa = 3.85 NMIYY373 pKa = 10.34 DD374 pKa = 4.68 PKK376 pKa = 10.81 TPLAGVAGPIFDD388 pKa = 3.8 VMSTYY393 pKa = 10.27 FVPTLNGEE401 pKa = 4.17 MDD403 pKa = 3.6 PSEE406 pKa = 4.14 AVEE409 pKa = 4.4 EE410 pKa = 4.1 ITYY413 pKa = 9.97 EE414 pKa = 4.08 LNDD417 pKa = 3.42 MM418 pKa = 4.77
Molecular weight: 45.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.704
IPC2_protein 3.732
IPC_protein 3.745
Toseland 3.528
ProMoST 3.884
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.617
Rodwell 3.567
Grimsley 3.439
Solomon 3.706
Lehninger 3.668
Nozaki 3.821
DTASelect 4.012
Thurlkill 3.567
EMBOSS 3.63
Sillero 3.859
Patrickios 1.252
IPC_peptide 3.706
IPC2_peptide 3.834
IPC2.peptide.svr19 3.764
Protein with the highest isoelectric point:
>tr|A0A4Q3YZ16|A0A4Q3YZ16_9RHOB Gfo/Idh/MocA family oxidoreductase OS=Salipiger sp. IMCC34102 OX=2510647 GN=EU805_03875 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNRR10 pKa = 11.84 VRR12 pKa = 11.84 KK13 pKa = 8.99 NRR15 pKa = 11.84 HH16 pKa = 3.77 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 AGRR28 pKa = 11.84 KK29 pKa = 8.81 IINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.31 GRR39 pKa = 11.84 AKK41 pKa = 10.69 LSAA44 pKa = 3.92
Molecular weight: 5.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.676
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.398
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.148
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3341
0
3341
1060431
28
2168
317.4
34.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.713 ± 0.052
0.859 ± 0.015
6.643 ± 0.042
5.712 ± 0.041
3.575 ± 0.026
8.799 ± 0.046
1.983 ± 0.021
4.934 ± 0.028
2.602 ± 0.033
9.992 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.664 ± 0.022
2.349 ± 0.021
5.243 ± 0.036
3.144 ± 0.021
7.145 ± 0.041
4.928 ± 0.036
5.853 ± 0.027
7.387 ± 0.036
1.374 ± 0.021
2.102 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here