Parambassis ranga (Indian glassy fish)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Clupeocephala; Euteleosteomorpha; Neoteleostei;

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 35559 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6P7IB55|A0A6P7IB55_9TELE calcium/calmodulin-dependent protein kinase type 1-like OS=Parambassis ranga OX=210632 GN=LOC114436059 PE=3 SV=1
MM1 pKa = 7.28SSKK4 pKa = 10.88GGGLEE9 pKa = 3.71RR10 pKa = 11.84HH11 pKa = 5.94LASLPVISPFRR22 pKa = 11.84PEE24 pKa = 3.5QRR26 pKa = 11.84YY27 pKa = 6.26TCSARR32 pKa = 11.84VSSAEE37 pKa = 3.78GVQEE41 pKa = 3.96SDD43 pKa = 4.77RR44 pKa = 11.84PSDD47 pKa = 3.65DD48 pKa = 3.12QAASNQPSDD57 pKa = 3.52VQAACNQPSDD67 pKa = 4.17DD68 pKa = 3.91QAASNQPSDD77 pKa = 3.52VQAACNQPPSDD88 pKa = 3.94NQAACNQPSDD98 pKa = 3.7NQAACNQPSDD108 pKa = 3.7VQAACNQPSDD118 pKa = 4.17DD119 pKa = 3.91QAASNQPSDD128 pKa = 4.46DD129 pKa = 3.55QAACNQPADD138 pKa = 4.13DD139 pKa = 4.22QAASNQPSDD148 pKa = 3.52VQAACNQPSDD158 pKa = 4.17DD159 pKa = 3.91QAASNQPSDD168 pKa = 3.55NQAACNQPADD178 pKa = 4.19DD179 pKa = 4.82QAACNQPSDD188 pKa = 3.7VQAASNQPVTSPQTTRR204 pKa = 11.84QPVTSPQTTRR214 pKa = 11.84QPVTSPQTTRR224 pKa = 11.84QPVTSPQTTRR234 pKa = 11.84QPVTSLL240 pKa = 3.17

Molecular weight: 25.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6P7J7L1|A0A6P7J7L1_9TELE uncharacterized protein LOC114443065 OS=Parambassis ranga OX=210632 GN=LOC114443065 PE=4 SV=1
MM1 pKa = 7.57SSHH4 pKa = 5.15KK5 pKa = 8.91TFRR8 pKa = 11.84IKK10 pKa = 10.64RR11 pKa = 11.84FLAKK15 pKa = 9.71KK16 pKa = 9.58QKK18 pKa = 8.69QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84MKK30 pKa = 9.89TGNKK34 pKa = 8.61IRR36 pKa = 11.84YY37 pKa = 7.09NSKK40 pKa = 8.3RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 3.95WRR45 pKa = 11.84RR46 pKa = 11.84TKK48 pKa = 10.83LGLL51 pKa = 3.67

Molecular weight: 6.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

22837

12722

35559

26690903

31

30257

750.6

83.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.62 ± 0.01

2.1 ± 0.011

5.296 ± 0.008

7.158 ± 0.02

3.277 ± 0.01

6.22 ± 0.018

2.64 ± 0.007

4.205 ± 0.009

5.673 ± 0.017

9.248 ± 0.017

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.322 ± 0.006

3.789 ± 0.01

5.998 ± 0.018

5.0 ± 0.015

5.607 ± 0.012

9.088 ± 0.018

5.896 ± 0.018

6.216 ± 0.011

1.054 ± 0.004

2.59 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski