Peribacillus loiseleuriae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Peribacillus

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4559 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K9GSV8|A0A0K9GSV8_9BACI Serine-type D-Ala-D-Ala carboxypeptidase OS=Peribacillus loiseleuriae OX=1679170 GN=AC625_07260 PE=3 SV=1
MM1 pKa = 7.22AQIGNCCNDD10 pKa = 3.14SLVGVNDD17 pKa = 4.3ALCFTIDD24 pKa = 3.91IADD27 pKa = 3.73TDD29 pKa = 4.12GTPLVLWNDD38 pKa = 2.72ATTFVINGTIMVEE51 pKa = 4.03NNGTIGVSPTAALTVNGTPIAGFIVGPGEE80 pKa = 4.26AKK82 pKa = 10.39SVTLNDD88 pKa = 3.35INSIGIIGTGGTPAGSTASVKK109 pKa = 10.5VSFSLNYY116 pKa = 10.21KK117 pKa = 9.89FF118 pKa = 5.4

Molecular weight: 11.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K9G523|A0A0K9G523_9BACI Uncharacterized protein OS=Peribacillus loiseleuriae OX=1679170 GN=AC625_24910 PE=4 SV=1
MM1 pKa = 6.95TQTSRR6 pKa = 11.84TIPGAAVRR14 pKa = 11.84GTASGVFFMAFFGTLWAYY32 pKa = 8.68TGIMGLQGWGVPLLLVAAVTIGIALFIGGVSLIRR66 pKa = 11.84ASRR69 pKa = 11.84EE70 pKa = 3.59LTNQVSKK77 pKa = 10.28TDD79 pKa = 3.34LRR81 pKa = 11.84RR82 pKa = 11.84GKK84 pKa = 8.89RR85 pKa = 11.84TRR87 pKa = 11.84FWFNIIFAAEE97 pKa = 3.99GLAIAITIVVCNATCHH113 pKa = 6.4SEE115 pKa = 4.57LIPPVIAIIVGVHH128 pKa = 6.14FLPLAPLFQVRR139 pKa = 11.84LYY141 pKa = 10.34YY142 pKa = 9.5FTGALLCLLAIITLLVVPAKK162 pKa = 9.56VTLGEE167 pKa = 4.09HH168 pKa = 6.14QINAFMSVVGFGSALILWGTGLAIWLMGRR197 pKa = 11.84RR198 pKa = 11.84LLGIIPQQ205 pKa = 3.56

Molecular weight: 22.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4559

0

4559

1304274

26

2156

286.1

32.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.847 ± 0.039

0.709 ± 0.011

4.937 ± 0.026

7.294 ± 0.042

4.6 ± 0.031

6.875 ± 0.039

2.048 ± 0.019

8.278 ± 0.041

7.001 ± 0.035

9.81 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.807 ± 0.017

4.568 ± 0.024

3.505 ± 0.023

3.689 ± 0.032

3.858 ± 0.029

6.197 ± 0.027

5.505 ± 0.026

6.957 ± 0.027

0.993 ± 0.013

3.521 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski