Amino acid dipepetide frequency for Peribacillus loiseleuriae

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.536AlaAla: 5.536 ± 0.08	0.623AlaCys: 0.623 ± 0.024	3.171AlaAsp: 3.171 ± 0.051	4.504AlaGlu: 4.504 ± 0.057	3.228AlaPhe: 3.228 ± 0.055	5.137AlaGly: 5.137 ± 0.074	1.236AlaHis: 1.236 ± 0.026	6.139AlaIle: 6.139 ± 0.08	4.697AlaLys: 4.697 ± 0.059	7.055AlaLeu: 7.055 ± 0.08	1.986AlaMet: 1.986 ± 0.038	2.917AlaAsn: 2.917 ± 0.049	1.915AlaPro: 1.915 ± 0.042	2.093AlaGln: 2.093 ± 0.039	2.468AlaArg: 2.468 ± 0.043	4.123AlaSer: 4.123 ± 0.068	3.606AlaThr: 3.606 ± 0.06	5.189AlaVal: 5.189 ± 0.068	0.57AlaTrp: 0.57 ± 0.023	2.273AlaTyr: 2.273 ± 0.039	0.001AlaXaa: 0.001 ± 0.001
Cys	0.438CysAla: 0.438 ± 0.02	0.102CysCys: 0.102 ± 0.01	0.38CysAsp: 0.38 ± 0.019	0.454CysGlu: 0.454 ± 0.017	0.344CysPhe: 0.344 ± 0.017	0.649CysGly: 0.649 ± 0.028	0.198CysHis: 0.198 ± 0.014	0.584CysIle: 0.584 ± 0.021	0.36CysLys: 0.36 ± 0.02	0.651CysLeu: 0.651 ± 0.022	0.191CysMet: 0.191 ± 0.012	0.294CysAsn: 0.294 ± 0.015	0.327CysPro: 0.327 ± 0.017	0.227CysGln: 0.227 ± 0.015	0.269CysArg: 0.269 ± 0.016	0.51CysSer: 0.51 ± 0.021	0.38CysThr: 0.38 ± 0.018	0.403CysVal: 0.403 ± 0.018	0.066CysTrp: 0.066 ± 0.008	0.264CysTyr: 0.264 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	3.108AspAla: 3.108 ± 0.059	0.383AspCys: 0.383 ± 0.016	2.307AspAsp: 2.307 ± 0.049	4.105AspGlu: 4.105 ± 0.068	2.462AspPhe: 2.462 ± 0.048	3.291AspGly: 3.291 ± 0.053	1.114AspHis: 1.114 ± 0.028	4.147AspIle: 4.147 ± 0.057	3.11AspLys: 3.11 ± 0.053	4.856AspLeu: 4.856 ± 0.059	1.369AspMet: 1.369 ± 0.032	1.868AspAsn: 1.868 ± 0.042	1.858AspPro: 1.858 ± 0.035	1.891AspGln: 1.891 ± 0.042	2.044AspArg: 2.044 ± 0.041	2.692AspSer: 2.692 ± 0.051	2.387AspThr: 2.387 ± 0.044	3.667AspVal: 3.667 ± 0.059	0.58AspTrp: 0.58 ± 0.021	2.131AspTyr: 2.131 ± 0.046	0.0AspXaa: 0.0 ± 0.0
Glu	5.04GluAla: 5.04 ± 0.071	0.391GluCys: 0.391 ± 0.017	3.368GluAsp: 3.368 ± 0.051	6.382GluGlu: 6.382 ± 0.103	2.73GluPhe: 2.73 ± 0.048	4.129GluGly: 4.129 ± 0.06	1.415GluHis: 1.415 ± 0.032	5.898GluIle: 5.898 ± 0.075	6.46GluLys: 6.46 ± 0.076	7.166GluLeu: 7.166 ± 0.119	2.344GluMet: 2.344 ± 0.043	3.767GluAsn: 3.767 ± 0.055	1.863GluPro: 1.863 ± 0.041	3.177GluGln: 3.177 ± 0.061	3.223GluArg: 3.223 ± 0.056	3.66GluSer: 3.66 ± 0.061	3.834GluThr: 3.834 ± 0.06	4.918GluVal: 4.918 ± 0.063	0.797GluTrp: 0.797 ± 0.028	2.383GluTyr: 2.383 ± 0.043	0.0GluXaa: 0.0 ± 0.0
Phe	3.1PheAla: 3.1 ± 0.047	0.378PheCys: 0.378 ± 0.016	2.341PheAsp: 2.341 ± 0.043	2.861PheGlu: 2.861 ± 0.044	2.354PhePhe: 2.354 ± 0.055	3.415PheGly: 3.415 ± 0.061	0.986PheHis: 0.986 ± 0.033	4.084PheIle: 4.084 ± 0.061	2.536PheLys: 2.536 ± 0.044	4.588PheLeu: 4.588 ± 0.079	1.232PheMet: 1.232 ± 0.035	2.012PheAsn: 2.012 ± 0.039	1.694PhePro: 1.694 ± 0.042	1.576PheGln: 1.576 ± 0.036	1.513PheArg: 1.513 ± 0.034	3.279PheSer: 3.279 ± 0.057	2.723PheThr: 2.723 ± 0.053	3.213PheVal: 3.213 ± 0.051	0.454PheTrp: 0.454 ± 0.019	1.664PheTyr: 1.664 ± 0.037	0.0PheXaa: 0.0 ± 0.0
Gly	4.838GlyAla: 4.838 ± 0.086	0.617GlyCys: 0.617 ± 0.02	3.05GlyAsp: 3.05 ± 0.062	4.446GlyGlu: 4.446 ± 0.062	3.419GlyPhe: 3.419 ± 0.057	4.774GlyGly: 4.774 ± 0.079	1.329GlyHis: 1.329 ± 0.034	6.439GlyIle: 6.439 ± 0.077	5.103GlyLys: 5.103 ± 0.069	6.671GlyLeu: 6.671 ± 0.078	2.115GlyMet: 2.115 ± 0.041	2.891GlyAsn: 2.891 ± 0.049	1.715GlyPro: 1.715 ± 0.04	2.148GlyGln: 2.148 ± 0.048	2.537GlyArg: 2.537 ± 0.054	4.124GlySer: 4.124 ± 0.065	4.021GlyThr: 4.021 ± 0.068	4.974GlyVal: 4.974 ± 0.069	0.781GlyTrp: 0.781 ± 0.027	2.758GlyTyr: 2.758 ± 0.053	0.001GlyXaa: 0.001 ± 0.001
His	1.269HisAla: 1.269 ± 0.032	0.198HisCys: 0.198 ± 0.013	1.063HisAsp: 1.063 ± 0.028	1.392HisGlu: 1.392 ± 0.032	1.078HisPhe: 1.078 ± 0.029	1.32HisGly: 1.32 ± 0.035	0.662HisHis: 0.662 ± 0.029	1.528HisIle: 1.528 ± 0.035	1.024HisLys: 1.024 ± 0.028	2.099HisLeu: 2.099 ± 0.04	0.497HisMet: 0.497 ± 0.02	0.764HisAsn: 0.764 ± 0.024	1.09HisPro: 1.09 ± 0.031	0.761HisGln: 0.761 ± 0.024	0.842HisArg: 0.842 ± 0.023	1.285HisSer: 1.285 ± 0.035	1.099HisThr: 1.099 ± 0.027	1.429HisVal: 1.429 ± 0.037	0.199HisTrp: 0.199 ± 0.012	0.881HisTyr: 0.881 ± 0.028	0.0HisXaa: 0.0 ± 0.0
Ile	6.239IleAla: 6.239 ± 0.082	0.652IleCys: 0.652 ± 0.023	4.38IleAsp: 4.38 ± 0.06	5.985IleGlu: 5.985 ± 0.073	3.502IlePhe: 3.502 ± 0.064	6.368IleGly: 6.368 ± 0.07	1.796IleHis: 1.796 ± 0.037	6.628IleIle: 6.628 ± 0.094	4.931IleLys: 4.931 ± 0.069	7.694IleLeu: 7.694 ± 0.099	1.99IleMet: 1.99 ± 0.038	3.491IleAsn: 3.491 ± 0.06	3.508IlePro: 3.508 ± 0.06	3.146IleGln: 3.146 ± 0.051	3.144IleArg: 3.144 ± 0.055	5.551IleSer: 5.551 ± 0.073	4.698IleThr: 4.698 ± 0.063	5.81IleVal: 5.81 ± 0.072	0.702IleTrp: 0.702 ± 0.027	2.562IleTyr: 2.562 ± 0.046	0.0IleXaa: 0.0 ± 0.0
Lys	4.586LysAla: 4.586 ± 0.066	0.307LysCys: 0.307 ± 0.018	3.708LysAsp: 3.708 ± 0.056	6.721LysGlu: 6.721 ± 0.083	2.081LysPhe: 2.081 ± 0.034	4.515LysGly: 4.515 ± 0.074	1.263LysHis: 1.263 ± 0.031	5.072LysIle: 5.072 ± 0.068	5.951LysLys: 5.951 ± 0.074	6.025LysLeu: 6.025 ± 0.072	2.335LysMet: 2.335 ± 0.045	3.481LysAsn: 3.481 ± 0.058	2.185LysPro: 2.185 ± 0.04	3.29LysGln: 3.29 ± 0.056	3.199LysArg: 3.199 ± 0.053	3.813LysSer: 3.813 ± 0.061	3.669LysThr: 3.669 ± 0.057	4.734LysVal: 4.734 ± 0.061	0.83LysTrp: 0.83 ± 0.025	2.243LysTyr: 2.243 ± 0.041	0.0LysXaa: 0.0 ± 0.0
Leu	7.192LeuAla: 7.192 ± 0.079	0.674LeuCys: 0.674 ± 0.022	4.87LeuAsp: 4.87 ± 0.062	6.456LeuGlu: 6.456 ± 0.095	4.876LeuPhe: 4.876 ± 0.084	6.528LeuGly: 6.528 ± 0.077	1.962LeuHis: 1.962 ± 0.04	7.515LeuIle: 7.515 ± 0.104	6.745LeuLys: 6.745 ± 0.081	10.415LeuLeu: 10.415 ± 0.112	2.463LeuMet: 2.463 ± 0.039	4.448LeuAsn: 4.448 ± 0.058	3.829LeuPro: 3.829 ± 0.05	3.588LeuGln: 3.588 ± 0.064	3.489LeuArg: 3.489 ± 0.052	6.941LeuSer: 6.941 ± 0.078	5.774LeuThr: 5.774 ± 0.067	6.433LeuVal: 6.433 ± 0.075	0.806LeuTrp: 0.806 ± 0.027	3.101LeuTyr: 3.101 ± 0.056	0.0LeuXaa: 0.0 ± 0.0
Met	2.022MetAla: 2.022 ± 0.042	0.143MetCys: 0.143 ± 0.009	1.506MetAsp: 1.506 ± 0.037	1.927MetGlu: 1.927 ± 0.037	1.057MetPhe: 1.057 ± 0.033	1.804MetGly: 1.804 ± 0.043	0.43MetHis: 0.43 ± 0.02	2.369MetIle: 2.369 ± 0.045	2.583MetLys: 2.583 ± 0.044	2.558MetLeu: 2.558 ± 0.045	0.876MetMet: 0.876 ± 0.029	1.719MetAsn: 1.719 ± 0.037	0.97MetPro: 0.97 ± 0.027	0.869MetGln: 0.869 ± 0.026	1.03MetArg: 1.03 ± 0.024	1.732MetSer: 1.732 ± 0.04	1.661MetThr: 1.661 ± 0.029	1.84MetVal: 1.84 ± 0.04	0.182MetTrp: 0.182 ± 0.011	0.794MetTyr: 0.794 ± 0.025	0.0MetXaa: 0.0 ± 0.0
Asn	2.742AsnAla: 2.742 ± 0.051	0.283AsnCys: 0.283 ± 0.017	2.236AsnAsp: 2.236 ± 0.042	3.627AsnGlu: 3.627 ± 0.055	1.733AsnPhe: 1.733 ± 0.039	3.451AsnGly: 3.451 ± 0.06	1.142AsnHis: 1.142 ± 0.032	3.6AsnIle: 3.6 ± 0.046	3.167AsnLys: 3.167 ± 0.057	3.982AsnLeu: 3.982 ± 0.053	1.236AsnMet: 1.236 ± 0.036	2.06AsnAsn: 2.06 ± 0.043	2.227AsnPro: 2.227 ± 0.045	2.135AsnGln: 2.135 ± 0.052	2.079AsnArg: 2.079 ± 0.039	2.526AsnSer: 2.526 ± 0.051	2.244AsnThr: 2.244 ± 0.038	3.053AsnVal: 3.053 ± 0.049	0.536AsnTrp: 0.536 ± 0.021	1.622AsnTyr: 1.622 ± 0.036	0.0AsnXaa: 0.0 ± 0.0
Pro	2.129ProAla: 2.129 ± 0.041	0.196ProCys: 0.196 ± 0.013	1.963ProAsp: 1.963 ± 0.035	2.836ProGlu: 2.836 ± 0.051	1.994ProPhe: 1.994 ± 0.04	2.175ProGly: 2.175 ± 0.053	0.766ProHis: 0.766 ± 0.025	3.033ProIle: 3.033 ± 0.051	2.155ProLys: 2.155 ± 0.044	3.423ProLeu: 3.423 ± 0.051	0.843ProMet: 0.843 ± 0.028	1.734ProAsn: 1.734 ± 0.037	0.889ProPro: 0.889 ± 0.031	1.077ProGln: 1.077 ± 0.028	1.051ProArg: 1.051 ± 0.032	2.309ProSer: 2.309 ± 0.043	2.042ProThr: 2.042 ± 0.044	2.687ProVal: 2.687 ± 0.046	0.343ProTrp: 0.343 ± 0.018	1.405ProTyr: 1.405 ± 0.036	0.0ProXaa: 0.0 ± 0.0
Gln	2.66GlnAla: 2.66 ± 0.047	0.21GlnCys: 0.21 ± 0.015	1.708GlnAsp: 1.708 ± 0.042	2.788GlnGlu: 2.788 ± 0.076	1.603GlnPhe: 1.603 ± 0.037	2.258GlnGly: 2.258 ± 0.042	0.723GlnHis: 0.723 ± 0.022	2.831GlnIle: 2.831 ± 0.047	2.69GlnLys: 2.69 ± 0.043	3.75GlnLeu: 3.75 ± 0.059	1.096GlnMet: 1.096 ± 0.031	1.729GlnAsn: 1.729 ± 0.049	1.244GlnPro: 1.244 ± 0.042	1.618GlnGln: 1.618 ± 0.044	1.485GlnArg: 1.485 ± 0.037	2.25GlnSer: 2.25 ± 0.06	2.04GlnThr: 2.04 ± 0.04	2.406GlnVal: 2.406 ± 0.038	0.399GlnTrp: 0.399 ± 0.018	1.406GlnTyr: 1.406 ± 0.036	0.0GlnXaa: 0.0 ± 0.0
Arg	2.307ArgAla: 2.307 ± 0.042	0.242ArgCys: 0.242 ± 0.013	1.928ArgAsp: 1.928 ± 0.041	2.996ArgGlu: 2.996 ± 0.042	1.878ArgPhe: 1.878 ± 0.033	2.279ArgGly: 2.279 ± 0.042	0.743ArgHis: 0.743 ± 0.024	3.167ArgIle: 3.167 ± 0.053	3.19ArgLys: 3.19 ± 0.053	3.82ArgLeu: 3.82 ± 0.062	1.294ArgMet: 1.294 ± 0.033	1.944ArgAsn: 1.944 ± 0.039	1.228ArgPro: 1.228 ± 0.027	1.379ArgGln: 1.379 ± 0.034	1.65ArgArg: 1.65 ± 0.034	2.137ArgSer: 2.137 ± 0.042	2.062ArgThr: 2.062 ± 0.041	2.472ArgVal: 2.472 ± 0.052	0.355ArgTrp: 0.355 ± 0.016	1.506ArgTyr: 1.506 ± 0.036	0.0ArgXaa: 0.0 ± 0.0
Ser	3.777SerAla: 3.777 ± 0.059	0.393SerCys: 0.393 ± 0.018	2.87SerAsp: 2.87 ± 0.053	4.107SerGlu: 4.107 ± 0.058	3.452SerPhe: 3.452 ± 0.06	4.586SerGly: 4.586 ± 0.057	1.247SerHis: 1.247 ± 0.034	5.465SerIle: 5.465 ± 0.072	4.048SerLys: 4.048 ± 0.057	6.367SerLeu: 6.367 ± 0.072	1.783SerMet: 1.783 ± 0.035	2.686SerAsn: 2.686 ± 0.04	2.11SerPro: 2.11 ± 0.044	2.083SerGln: 2.083 ± 0.046	2.293SerArg: 2.293 ± 0.049	4.044SerSer: 4.044 ± 0.07	3.36SerThr: 3.36 ± 0.059	4.332SerVal: 4.332 ± 0.063	0.676SerTrp: 0.676 ± 0.03	2.29SerTyr: 2.29 ± 0.049	0.0SerXaa: 0.0 ± 0.0
Thr	3.873ThrAla: 3.873 ± 0.061	0.357ThrCys: 0.357 ± 0.018	2.71ThrAsp: 2.71 ± 0.048	3.541ThrGlu: 3.541 ± 0.054	2.716ThrPhe: 2.716 ± 0.051	4.271ThrGly: 4.271 ± 0.092	1.057ThrHis: 1.057 ± 0.028	4.914ThrIle: 4.914 ± 0.062	3.575ThrLys: 3.575 ± 0.05	5.392ThrLeu: 5.392 ± 0.061	1.391ThrMet: 1.391 ± 0.032	2.568ThrAsn: 2.568 ± 0.045	2.19ThrPro: 2.19 ± 0.04	1.498ThrGln: 1.498 ± 0.035	1.775ThrArg: 1.775 ± 0.035	3.446ThrSer: 3.446 ± 0.049	2.981ThrThr: 2.981 ± 0.051	4.318ThrVal: 4.318 ± 0.067	0.548ThrTrp: 0.548 ± 0.022	1.926ThrTyr: 1.926 ± 0.034	0.001ThrXaa: 0.001 ± 0.001
Val	4.981ValAla: 4.981 ± 0.067	0.564ValCys: 0.564 ± 0.024	3.499ValAsp: 3.499 ± 0.057	4.708ValGlu: 4.708 ± 0.063	3.15ValPhe: 3.15 ± 0.053	4.624ValGly: 4.624 ± 0.075	1.35ValHis: 1.35 ± 0.037	5.988ValIle: 5.988 ± 0.069	4.801ValLys: 4.801 ± 0.06	6.829ValLeu: 6.829 ± 0.08	1.848ValMet: 1.848 ± 0.039	3.236ValAsn: 3.236 ± 0.055	2.515ValPro: 2.515 ± 0.043	2.368ValGln: 2.368 ± 0.043	2.529ValArg: 2.529 ± 0.049	4.711ValSer: 4.711 ± 0.065	4.158ValThr: 4.158 ± 0.062	4.833ValVal: 4.833 ± 0.073	0.6ValTrp: 0.6 ± 0.024	2.273ValTyr: 2.273 ± 0.043	0.0ValXaa: 0.0 ± 0.0
Trp	0.612TrpAla: 0.612 ± 0.023	0.068TrpCys: 0.068 ± 0.007	0.48TrpAsp: 0.48 ± 0.02	0.586TrpGlu: 0.586 ± 0.022	0.531TrpPhe: 0.531 ± 0.022	0.674TrpGly: 0.674 ± 0.025	0.196TrpHis: 0.196 ± 0.012	0.856TrpIle: 0.856 ± 0.025	0.711TrpLys: 0.711 ± 0.026	1.126TrpLeu: 1.126 ± 0.036	0.307TrpMet: 0.307 ± 0.014	0.576TrpAsn: 0.576 ± 0.021	0.252TrpPro: 0.252 ± 0.015	0.36TrpGln: 0.36 ± 0.018	0.383TrpArg: 0.383 ± 0.017	0.623TrpSer: 0.623 ± 0.02	0.491TrpThr: 0.491 ± 0.021	0.641TrpVal: 0.641 ± 0.022	0.124TrpTrp: 0.124 ± 0.01	0.333TrpTyr: 0.333 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.018TyrAla: 2.018 ± 0.038	0.311TyrCys: 0.311 ± 0.015	1.83TyrAsp: 1.83 ± 0.038	2.523TyrGlu: 2.523 ± 0.048	1.815TyrPhe: 1.815 ± 0.039	2.506TyrGly: 2.506 ± 0.048	0.866TyrHis: 0.866 ± 0.03	2.519TyrIle: 2.519 ± 0.052	2.171TyrLys: 2.171 ± 0.048	3.638TyrLeu: 3.638 ± 0.058	0.878TyrMet: 0.878 ± 0.026	1.498TyrAsn: 1.498 ± 0.034	1.44TyrPro: 1.44 ± 0.034	1.611TyrGln: 1.611 ± 0.042	1.573TyrArg: 1.573 ± 0.035	2.213TyrSer: 2.213 ± 0.044	1.815TyrThr: 1.815 ± 0.037	2.213TyrVal: 2.213 ± 0.047	0.381TyrTrp: 0.381 ± 0.021	1.398TyrTyr: 1.398 ± 0.037	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.001	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.001XaaVal: 0.001 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.012XaaXaa: 0.012 ± 0.009

Statistics based on 4559 proteins (1304274 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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