Mycobacterium sp. NAZ190054

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Mycobacteriaceae; Mycobacterium; unclassified Mycobacterium

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5363 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A132T6L2|A0A132T6L2_9MYCO ESAT-6-like protein OS=Mycobacterium sp. NAZ190054 OX=1747766 GN=ASJ79_23350 PE=3 SV=1
MM1 pKa = 7.24HH2 pKa = 7.22WTRR5 pKa = 11.84PLAALSALGLLMTTVGCTTQVSGVARR31 pKa = 11.84VDD33 pKa = 3.32PTAAPLALTEE43 pKa = 4.91DD44 pKa = 3.94GFGVVAGFDD53 pKa = 3.6DD54 pKa = 4.82APAKK58 pKa = 10.36IEE60 pKa = 4.45IYY62 pKa = 9.98TEE64 pKa = 4.37PQCSHH69 pKa = 6.9CSDD72 pKa = 3.67LQYY75 pKa = 11.6DD76 pKa = 4.39FGDD79 pKa = 3.42EE80 pKa = 3.63LAYY83 pKa = 10.88NITVGTLQVTYY94 pKa = 10.63RR95 pKa = 11.84PLTFLDD101 pKa = 4.07DD102 pKa = 4.61DD103 pKa = 4.76YY104 pKa = 11.79NGYY107 pKa = 8.03SAKK110 pKa = 10.22VANALFLAADD120 pKa = 3.77AVGDD124 pKa = 4.03SAATGTQLQRR134 pKa = 11.84FVYY137 pKa = 8.97EE138 pKa = 3.68LWINQQPGGPEE149 pKa = 3.7FTGDD153 pKa = 3.72EE154 pKa = 4.52LRR156 pKa = 11.84DD157 pKa = 3.53MAADD161 pKa = 3.49AGVPDD166 pKa = 4.37VVADD170 pKa = 4.6HH171 pKa = 7.05IGTDD175 pKa = 3.5AEE177 pKa = 4.23AVDD180 pKa = 4.19VVEE183 pKa = 4.05MDD185 pKa = 3.38EE186 pKa = 4.65TNFDD190 pKa = 4.22LLFDD194 pKa = 4.0IDD196 pKa = 3.84RR197 pKa = 11.84VEE199 pKa = 4.25TGTPTVFDD207 pKa = 4.71LNAGEE212 pKa = 5.52KK213 pKa = 9.58IDD215 pKa = 4.63IYY217 pKa = 11.4DD218 pKa = 4.32DD219 pKa = 3.05AWLADD224 pKa = 3.95LVAGG228 pKa = 4.82

Molecular weight: 24.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A132T752|A0A132T752_9MYCO Hydrolase OS=Mycobacterium sp. NAZ190054 OX=1747766 GN=ASJ79_22950 PE=4 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84AKK17 pKa = 9.45VHH19 pKa = 5.31GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVTARR37 pKa = 11.84RR38 pKa = 11.84AKK40 pKa = 10.16GRR42 pKa = 11.84RR43 pKa = 11.84SLTAA47 pKa = 3.9

Molecular weight: 5.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5363

0

5363

1672165

37

2846

311.8

33.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.75 ± 0.04

0.816 ± 0.01

6.468 ± 0.03

5.45 ± 0.027

3.16 ± 0.019

8.921 ± 0.032

2.188 ± 0.017

4.23 ± 0.025

2.125 ± 0.023

9.77 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.115 ± 0.014

2.158 ± 0.018

5.693 ± 0.023

2.879 ± 0.017

7.312 ± 0.033

5.285 ± 0.021

6.077 ± 0.024

8.952 ± 0.03

1.485 ± 0.013

2.166 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski