Pseudoflavonifractor sp. An176

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Pseudoflavonifractor; unclassified Pseudoflavonifractor

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2212 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1Y4M1G9|A0A1Y4M1G9_9FIRM Polyketide cyclase OS=Pseudoflavonifractor sp. An176 OX=1965572 GN=B5F12_09745 PE=4 SV=1
MM1 pKa = 7.03ATAAPGVLAYY11 pKa = 10.05AAEE14 pKa = 4.18AQTRR18 pKa = 11.84EE19 pKa = 3.95NADD22 pKa = 3.52NLEE25 pKa = 4.26LSDD28 pKa = 4.71GYY30 pKa = 11.33LSVSMSTEE38 pKa = 3.55NGGFLVNTEE47 pKa = 4.66EE48 pKa = 4.39GDD50 pKa = 4.05KK51 pKa = 11.26LNKK54 pKa = 9.8SDD56 pKa = 3.79NNKK59 pKa = 9.81NLLYY63 pKa = 10.04PAADD67 pKa = 3.61YY68 pKa = 7.52DD69 pKa = 3.81TSYY72 pKa = 11.0TSVRR76 pKa = 11.84VTRR79 pKa = 11.84TDD81 pKa = 3.0GSTEE85 pKa = 4.02DD86 pKa = 3.67YY87 pKa = 11.42VFGRR91 pKa = 11.84DD92 pKa = 3.38YY93 pKa = 11.68GFFGLDD99 pKa = 3.14SSDD102 pKa = 3.84VEE104 pKa = 4.43VSKK107 pKa = 11.19EE108 pKa = 3.91GNTLKK113 pKa = 10.82AQWSVKK119 pKa = 10.21DD120 pKa = 3.41LTMVQTLSLLDD131 pKa = 3.63QNSAQHH137 pKa = 5.74GMVSISYY144 pKa = 9.65QVSTDD149 pKa = 3.37RR150 pKa = 11.84DD151 pKa = 3.57DD152 pKa = 3.94VEE154 pKa = 4.35NVKK157 pKa = 10.41LRR159 pKa = 11.84LMLDD163 pKa = 3.37TALGYY168 pKa = 9.82QDD170 pKa = 3.01WATYY174 pKa = 8.52EE175 pKa = 4.02LPGVNGIYY183 pKa = 8.73QHH185 pKa = 6.05ITSEE189 pKa = 4.16QVLDD193 pKa = 3.85NRR195 pKa = 11.84DD196 pKa = 3.16GQAYY200 pKa = 8.97SGAMFAVDD208 pKa = 4.17DD209 pKa = 4.69ANAPSMTAYY218 pKa = 8.9TVNASLDD225 pKa = 3.78GQTVEE230 pKa = 4.71PYY232 pKa = 10.5QLAFGHH238 pKa = 6.37WNNLAEE244 pKa = 4.31TSFDD248 pKa = 3.59FTPDD252 pKa = 3.12LGLDD256 pKa = 3.52FTNPYY261 pKa = 10.41NEE263 pKa = 4.67TYY265 pKa = 9.29LTADD269 pKa = 2.93SAYY272 pKa = 10.78ALYY275 pKa = 10.82YY276 pKa = 10.53DD277 pKa = 5.22LGQITPDD284 pKa = 3.58DD285 pKa = 4.02SAVMSTYY292 pKa = 10.49YY293 pKa = 10.59GVYY296 pKa = 10.03SNVTVGEE303 pKa = 4.14EE304 pKa = 3.39EE305 pKa = 3.67RR306 pKa = 11.84AAINFPEE313 pKa = 5.23LPASMTFNEE322 pKa = 4.32EE323 pKa = 2.96QDD325 pKa = 3.88AYY327 pKa = 11.0LSQVEE332 pKa = 4.7GGAEE336 pKa = 4.13GDD338 pKa = 3.42VHH340 pKa = 6.9LQVSVSNISDD350 pKa = 3.69DD351 pKa = 4.37AVSQMAVVTRR361 pKa = 11.84VRR363 pKa = 11.84NGVQPYY369 pKa = 8.27EE370 pKa = 4.23DD371 pKa = 4.33YY372 pKa = 11.61YY373 pKa = 11.31EE374 pKa = 4.4GLLYY378 pKa = 10.76EE379 pKa = 4.82GGARR383 pKa = 11.84EE384 pKa = 4.71DD385 pKa = 4.37FYY387 pKa = 11.7QVVRR391 pKa = 11.84DD392 pKa = 4.2FQPGEE397 pKa = 3.74EE398 pKa = 4.22AQVEE402 pKa = 4.85FYY404 pKa = 11.44YY405 pKa = 10.83NVSPLPVSEE414 pKa = 3.94YY415 pKa = 10.62RR416 pKa = 11.84KK417 pKa = 10.3FEE419 pKa = 3.96ILCYY423 pKa = 10.28DD424 pKa = 3.22ISDD427 pKa = 3.65TGEE430 pKa = 4.17LLEE433 pKa = 4.26QNLLASRR440 pKa = 11.84EE441 pKa = 4.18FYY443 pKa = 10.8VLCPGVLGEE452 pKa = 4.59ILTFHH457 pKa = 6.88SLNPQIIYY465 pKa = 10.3TDD467 pKa = 3.68GTRR470 pKa = 11.84HH471 pKa = 6.43LFLSGQNFQLLQNTSAYY488 pKa = 8.49TVYY491 pKa = 10.65LKK493 pKa = 10.73SLSGGTDD500 pKa = 3.24LVLDD504 pKa = 3.87SSKK507 pKa = 10.97VFLDD511 pKa = 3.23MDD513 pKa = 4.4KK514 pKa = 11.46NSLDD518 pKa = 3.79LVVDD522 pKa = 3.33QHH524 pKa = 7.11LDD526 pKa = 3.17PGGYY530 pKa = 9.11QVVFNWAEE538 pKa = 3.84AGKK541 pKa = 10.53EE542 pKa = 4.0PTTSSALQFSVTEE555 pKa = 4.0EE556 pKa = 4.03PEE558 pKa = 4.39YY559 pKa = 10.72ISDD562 pKa = 3.74VYY564 pKa = 11.36GVVTIEE570 pKa = 4.22KK571 pKa = 10.26DD572 pKa = 3.62GEE574 pKa = 4.02DD575 pKa = 4.12GYY577 pKa = 11.91ALRR580 pKa = 11.84LYY582 pKa = 10.74DD583 pKa = 5.88DD584 pKa = 3.62EE585 pKa = 4.54TSYY588 pKa = 11.68DD589 pKa = 3.5RR590 pKa = 11.84SLTDD594 pKa = 3.37MEE596 pKa = 4.68KK597 pKa = 10.44QNQVLLEE604 pKa = 3.85FRR606 pKa = 11.84GDD608 pKa = 3.38FTAQYY613 pKa = 10.84DD614 pKa = 3.7EE615 pKa = 5.26DD616 pKa = 4.88GNLVSASAVSVEE628 pKa = 4.19AADD631 pKa = 4.04GTVSSTINISNCLDD645 pKa = 3.48VEE647 pKa = 4.52AGSLTIRR654 pKa = 11.84VEE656 pKa = 4.15NPGTEE661 pKa = 3.87EE662 pKa = 3.83QVINTDD668 pKa = 2.53IDD670 pKa = 3.82GKK672 pKa = 11.29VYY674 pKa = 7.6TTNSRR679 pKa = 11.84TKK681 pKa = 9.56VWNGVCAITSLEE693 pKa = 3.98NGEE696 pKa = 4.71EE697 pKa = 4.35YY698 pKa = 10.32PLLQYY703 pKa = 11.17DD704 pKa = 3.93YY705 pKa = 9.68MGNPTSDD712 pKa = 3.5VEE714 pKa = 4.23NSVANTNAITLVWPGAASTAQTLAGILFEE743 pKa = 5.33FRR745 pKa = 11.84YY746 pKa = 10.1CQFGLMALEE755 pKa = 4.67TGTVTDD761 pKa = 3.43QTPKK765 pKa = 10.22KK766 pKa = 9.9RR767 pKa = 11.84VVAFGAEE774 pKa = 3.94MSPDD778 pKa = 3.4FLLPSNFDD786 pKa = 2.7WGNRR790 pKa = 11.84QTSTLEE796 pKa = 3.76AVQLNLAKK804 pKa = 10.51SNYY807 pKa = 8.99TADD810 pKa = 3.41QLRR813 pKa = 11.84DD814 pKa = 3.41VQARR818 pKa = 11.84YY819 pKa = 8.97AADD822 pKa = 3.36QQAWEE827 pKa = 4.09EE828 pKa = 4.44AEE830 pKa = 4.16TGSLSLYY837 pKa = 9.57VHH839 pKa = 7.61DD840 pKa = 4.79ILFGGGFVGFNASVEE855 pKa = 4.36VALPSYY861 pKa = 11.36ADD863 pKa = 3.51GLPGVEE869 pKa = 3.92GTLNLRR875 pKa = 11.84VMPDD879 pKa = 3.08RR880 pKa = 11.84DD881 pKa = 3.28LWEE884 pKa = 4.11VGVYY888 pKa = 10.43GSADD892 pKa = 3.44FAILNMEE899 pKa = 4.39AEE901 pKa = 4.61LVITAYY907 pKa = 11.25NNIPLPNKK915 pKa = 9.25IYY917 pKa = 10.12FYY919 pKa = 11.3VGGFTPGINVDD930 pKa = 3.14GMGIFWIQGAGGGVEE945 pKa = 4.24GLADD949 pKa = 3.75TVFLSSRR956 pKa = 11.84VPPLKK961 pKa = 10.69LIFSGQFALFAVLQARR977 pKa = 11.84ADD979 pKa = 3.55LTMGMRR985 pKa = 11.84EE986 pKa = 3.97FSLGMRR992 pKa = 11.84DD993 pKa = 3.81LNIAGITLLEE1003 pKa = 4.44SASLTTQWYY1012 pKa = 8.95PEE1014 pKa = 3.93LRR1016 pKa = 11.84LNAGIEE1022 pKa = 4.08LNILDD1027 pKa = 4.8IIEE1030 pKa = 4.52GSGSIVLEE1038 pKa = 3.5EE1039 pKa = 4.38DD1040 pKa = 2.87KK1041 pKa = 11.28RR1042 pKa = 11.84EE1043 pKa = 3.91NRR1045 pKa = 11.84LFWEE1049 pKa = 5.37GFATAGVTTPKK1060 pKa = 10.11IPLIGEE1066 pKa = 3.7IHH1068 pKa = 6.79IGDD1071 pKa = 3.62VDD1073 pKa = 4.45LGLNASKK1080 pKa = 10.03IWGALHH1086 pKa = 6.97VLMLDD1091 pKa = 3.46MGVTYY1096 pKa = 10.66YY1097 pKa = 10.14WGGDD1101 pKa = 2.88VDD1103 pKa = 4.97FAFGKK1108 pKa = 10.71YY1109 pKa = 7.09NTPEE1113 pKa = 3.9ATVPAALSGIPVYY1126 pKa = 10.49TDD1128 pKa = 2.7AGTGRR1133 pKa = 11.84VLYY1136 pKa = 9.94MSVGTNARR1144 pKa = 11.84LEE1146 pKa = 4.19AATDD1150 pKa = 3.29RR1151 pKa = 11.84RR1152 pKa = 11.84TFDD1155 pKa = 3.51LAGTATRR1162 pKa = 11.84TNGEE1166 pKa = 3.94SSIDD1170 pKa = 3.46RR1171 pKa = 11.84SADD1174 pKa = 2.89GMTHH1178 pKa = 6.57VIQLGSYY1185 pKa = 9.66SGASGKK1191 pKa = 10.42ALTLSFQADD1200 pKa = 3.7SLEE1203 pKa = 4.13QAQRR1207 pKa = 11.84IAMGDD1212 pKa = 3.26SRR1214 pKa = 11.84YY1215 pKa = 7.71GTPGITLADD1224 pKa = 4.45PDD1226 pKa = 5.34DD1227 pKa = 4.86PGQTYY1232 pKa = 8.95SLRR1235 pKa = 11.84WLDD1238 pKa = 3.5TTQDD1242 pKa = 3.39AEE1244 pKa = 4.32EE1245 pKa = 4.13QSDD1248 pKa = 3.93ANALLSYY1255 pKa = 10.2DD1256 pKa = 3.89QEE1258 pKa = 4.44SGEE1261 pKa = 4.34ANVTISFTQAEE1272 pKa = 5.15DD1273 pKa = 3.29YY1274 pKa = 10.54HH1275 pKa = 7.79AWRR1278 pKa = 11.84LTLGASAQSALYY1290 pKa = 10.24DD1291 pKa = 3.43IAYY1294 pKa = 10.33LPGLDD1299 pKa = 3.55SASVQWNDD1307 pKa = 3.02QTQEE1311 pKa = 4.17LTVTWQGSEE1320 pKa = 4.1LNDD1323 pKa = 3.45VEE1325 pKa = 4.64NVAVYY1330 pKa = 10.04AVSQTEE1336 pKa = 4.26EE1337 pKa = 4.68GEE1339 pKa = 4.46STTTLLYY1346 pKa = 9.35EE1347 pKa = 4.45TDD1349 pKa = 3.43DD1350 pKa = 3.49TGIISGGVAGATQGSATFAVPTDD1373 pKa = 4.34LPSGSYY1379 pKa = 9.39TIQVVGSSEE1388 pKa = 4.01TRR1390 pKa = 11.84SVNDD1394 pKa = 3.19VVKK1397 pKa = 10.98VEE1399 pKa = 4.33EE1400 pKa = 4.23PLAYY1404 pKa = 9.32TNPNQPQAPVIDD1416 pKa = 4.23SVKK1419 pKa = 10.82LGGDD1423 pKa = 3.29YY1424 pKa = 10.95SIDD1427 pKa = 3.5VAVEE1431 pKa = 3.65KK1432 pKa = 10.82QGAYY1436 pKa = 10.02DD1437 pKa = 4.29GYY1439 pKa = 10.86VATIYY1444 pKa = 10.24EE1445 pKa = 4.2QTDD1448 pKa = 3.35TGLVATDD1455 pKa = 5.21LAQQWVEE1462 pKa = 4.12TSGEE1466 pKa = 4.11APEE1469 pKa = 4.19EE1470 pKa = 4.03TLTLGGRR1477 pKa = 11.84YY1478 pKa = 5.59TTSAYY1483 pKa = 10.84VDD1485 pKa = 3.49GDD1487 pKa = 3.91GNAVLPSEE1495 pKa = 4.63AEE1497 pKa = 3.89GRR1499 pKa = 11.84DD1500 pKa = 3.4DD1501 pKa = 3.62VTSVEE1506 pKa = 4.05QTVGVEE1512 pKa = 3.52AGKK1515 pKa = 10.58VYY1517 pKa = 10.71KK1518 pKa = 10.25IGLAGYY1524 pKa = 6.35TTGPNGEE1531 pKa = 4.28IFLSQEE1537 pKa = 4.32TLSSAITMQAPQKK1550 pKa = 10.35ATVTVQAEE1558 pKa = 4.64GSFSVDD1564 pKa = 2.58GTDD1567 pKa = 4.06TINGVNATIQITSDD1581 pKa = 3.52MPVSGTWTLDD1591 pKa = 2.84GDD1593 pKa = 4.16VASGQWQAGTDD1604 pKa = 3.88QNFLDD1609 pKa = 3.7QAIDD1613 pKa = 3.67FFANLFGGEE1622 pKa = 4.1RR1623 pKa = 11.84EE1624 pKa = 4.35QIGTIYY1630 pKa = 10.69LSGRR1634 pKa = 11.84AEE1636 pKa = 4.11TDD1638 pKa = 3.26VLPDD1642 pKa = 4.49DD1643 pKa = 4.96GGLTEE1648 pKa = 4.54GTHH1651 pKa = 5.75ILSLVGEE1658 pKa = 4.52NEE1660 pKa = 3.75QGDD1663 pKa = 4.42AVSVKK1668 pKa = 10.67YY1669 pKa = 8.45MFRR1672 pKa = 11.84VDD1674 pKa = 3.26TTAPRR1679 pKa = 11.84LQLISPADD1687 pKa = 3.37GDD1689 pKa = 4.24YY1690 pKa = 11.14FSDD1693 pKa = 3.46TVTVEE1698 pKa = 4.13GLSEE1702 pKa = 4.29PGAVVTVLVDD1712 pKa = 3.69GQAAQTVTIPEE1723 pKa = 4.76DD1724 pKa = 3.37GTLSVEE1730 pKa = 4.01IPVDD1734 pKa = 3.54NTVLEE1739 pKa = 4.48HH1740 pKa = 6.82EE1741 pKa = 4.29LTIYY1745 pKa = 11.09AEE1747 pKa = 4.22DD1748 pKa = 3.41AVGNVGRR1755 pKa = 11.84KK1756 pKa = 7.72MSIPLLNDD1764 pKa = 3.13IAAAPDD1770 pKa = 3.43VALGILFNGQDD1781 pKa = 3.67YY1782 pKa = 8.1TQQNIPAGTNGVVQLCLVSGSRR1804 pKa = 11.84RR1805 pKa = 11.84VPVPADD1811 pKa = 3.04SVMGRR1816 pKa = 11.84QAEE1819 pKa = 4.22WQTTVVKK1826 pKa = 9.63GTASLDD1832 pKa = 3.47NGLSLLTSLDD1842 pKa = 3.66VNGMLTVSLDD1852 pKa = 3.45QMQAAAVLGGNANDD1866 pKa = 5.11IDD1868 pKa = 4.21YY1869 pKa = 7.46QTCNVTVTANEE1880 pKa = 4.31GGTVSPAGTTAVTEE1894 pKa = 4.49GEE1896 pKa = 4.58SFTLTITPDD1905 pKa = 2.83AYY1907 pKa = 10.31YY1908 pKa = 9.92HH1909 pKa = 5.96VEE1911 pKa = 4.16SVVVNN1916 pKa = 4.14

Molecular weight:
206.49 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1Y4M6N6|A0A1Y4M6N6_9FIRM Uncharacterized protein OS=Pseudoflavonifractor sp. An176 OX=1965572 GN=B5F12_10555 PE=4 SV=1
MM1 pKa = 7.06VRR3 pKa = 11.84TYY5 pKa = 10.24QPKK8 pKa = 9.12KK9 pKa = 7.46RR10 pKa = 11.84HH11 pKa = 5.45RR12 pKa = 11.84SKK14 pKa = 10.49VHH16 pKa = 5.66GFRR19 pKa = 11.84KK20 pKa = 10.08RR21 pKa = 11.84MATANGRR28 pKa = 11.84KK29 pKa = 8.93VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.22GRR39 pKa = 11.84VRR41 pKa = 11.84LTHH44 pKa = 6.42

Molecular weight:
5.3 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2212

0

2212

726739

37

2834

328.5

36.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.971 ± 0.06

1.562 ± 0.026

5.652 ± 0.053

6.593 ± 0.053

3.585 ± 0.036

7.8 ± 0.051

2.033 ± 0.024

5.189 ± 0.043

4.522 ± 0.055

9.955 ± 0.071

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.849 ± 0.023

3.457 ± 0.04

4.175 ± 0.031

4.452 ± 0.042

5.145 ± 0.055

5.475 ± 0.044

5.996 ± 0.066

7.809 ± 0.044

1.141 ± 0.017

3.638 ± 0.04

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski