Pseudoflavonifractor sp. An176
Average proteome isoelectric point is 6.04
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2212 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Y4M1G9|A0A1Y4M1G9_9FIRM Polyketide cyclase OS=Pseudoflavonifractor sp. An176 OX=1965572 GN=B5F12_09745 PE=4 SV=1
MM1 pKa = 7.03 ATAAPGVLAYY11 pKa = 10.05 AAEE14 pKa = 4.18 AQTRR18 pKa = 11.84 EE19 pKa = 3.95 NADD22 pKa = 3.52 NLEE25 pKa = 4.26 LSDD28 pKa = 4.71 GYY30 pKa = 11.33 LSVSMSTEE38 pKa = 3.55 NGGFLVNTEE47 pKa = 4.66 EE48 pKa = 4.39 GDD50 pKa = 4.05 KK51 pKa = 11.26 LNKK54 pKa = 9.8 SDD56 pKa = 3.79 NNKK59 pKa = 9.81 NLLYY63 pKa = 10.04 PAADD67 pKa = 3.61 YY68 pKa = 7.52 DD69 pKa = 3.81 TSYY72 pKa = 11.0 TSVRR76 pKa = 11.84 VTRR79 pKa = 11.84 TDD81 pKa = 3.0 GSTEE85 pKa = 4.02 DD86 pKa = 3.67 YY87 pKa = 11.42 VFGRR91 pKa = 11.84 DD92 pKa = 3.38 YY93 pKa = 11.68 GFFGLDD99 pKa = 3.14 SSDD102 pKa = 3.84 VEE104 pKa = 4.43 VSKK107 pKa = 11.19 EE108 pKa = 3.91 GNTLKK113 pKa = 10.82 AQWSVKK119 pKa = 10.21 DD120 pKa = 3.41 LTMVQTLSLLDD131 pKa = 3.63 QNSAQHH137 pKa = 5.74 GMVSISYY144 pKa = 9.65 QVSTDD149 pKa = 3.37 RR150 pKa = 11.84 DD151 pKa = 3.57 DD152 pKa = 3.94 VEE154 pKa = 4.35 NVKK157 pKa = 10.41 LRR159 pKa = 11.84 LMLDD163 pKa = 3.37 TALGYY168 pKa = 9.82 QDD170 pKa = 3.01 WATYY174 pKa = 8.52 EE175 pKa = 4.02 LPGVNGIYY183 pKa = 8.73 QHH185 pKa = 6.05 ITSEE189 pKa = 4.16 QVLDD193 pKa = 3.85 NRR195 pKa = 11.84 DD196 pKa = 3.16 GQAYY200 pKa = 8.97 SGAMFAVDD208 pKa = 4.17 DD209 pKa = 4.69 ANAPSMTAYY218 pKa = 8.9 TVNASLDD225 pKa = 3.78 GQTVEE230 pKa = 4.71 PYY232 pKa = 10.5 QLAFGHH238 pKa = 6.37 WNNLAEE244 pKa = 4.31 TSFDD248 pKa = 3.59 FTPDD252 pKa = 3.12 LGLDD256 pKa = 3.52 FTNPYY261 pKa = 10.41 NEE263 pKa = 4.67 TYY265 pKa = 9.29 LTADD269 pKa = 2.93 SAYY272 pKa = 10.78 ALYY275 pKa = 10.82 YY276 pKa = 10.53 DD277 pKa = 5.22 LGQITPDD284 pKa = 3.58 DD285 pKa = 4.02 SAVMSTYY292 pKa = 10.49 YY293 pKa = 10.59 GVYY296 pKa = 10.03 SNVTVGEE303 pKa = 4.14 EE304 pKa = 3.39 EE305 pKa = 3.67 RR306 pKa = 11.84 AAINFPEE313 pKa = 5.23 LPASMTFNEE322 pKa = 4.32 EE323 pKa = 2.96 QDD325 pKa = 3.88 AYY327 pKa = 11.0 LSQVEE332 pKa = 4.7 GGAEE336 pKa = 4.13 GDD338 pKa = 3.42 VHH340 pKa = 6.9 LQVSVSNISDD350 pKa = 3.69 DD351 pKa = 4.37 AVSQMAVVTRR361 pKa = 11.84 VRR363 pKa = 11.84 NGVQPYY369 pKa = 8.27 EE370 pKa = 4.23 DD371 pKa = 4.33 YY372 pKa = 11.61 YY373 pKa = 11.31 EE374 pKa = 4.4 GLLYY378 pKa = 10.76 EE379 pKa = 4.82 GGARR383 pKa = 11.84 EE384 pKa = 4.71 DD385 pKa = 4.37 FYY387 pKa = 11.7 QVVRR391 pKa = 11.84 DD392 pKa = 4.2 FQPGEE397 pKa = 3.74 EE398 pKa = 4.22 AQVEE402 pKa = 4.85 FYY404 pKa = 11.44 YY405 pKa = 10.83 NVSPLPVSEE414 pKa = 3.94 YY415 pKa = 10.62 RR416 pKa = 11.84 KK417 pKa = 10.3 FEE419 pKa = 3.96 ILCYY423 pKa = 10.28 DD424 pKa = 3.22 ISDD427 pKa = 3.65 TGEE430 pKa = 4.17 LLEE433 pKa = 4.26 QNLLASRR440 pKa = 11.84 EE441 pKa = 4.18 FYY443 pKa = 10.8 VLCPGVLGEE452 pKa = 4.59 ILTFHH457 pKa = 6.88 SLNPQIIYY465 pKa = 10.3 TDD467 pKa = 3.68 GTRR470 pKa = 11.84 HH471 pKa = 6.43 LFLSGQNFQLLQNTSAYY488 pKa = 8.49 TVYY491 pKa = 10.65 LKK493 pKa = 10.73 SLSGGTDD500 pKa = 3.24 LVLDD504 pKa = 3.87 SSKK507 pKa = 10.97 VFLDD511 pKa = 3.23 MDD513 pKa = 4.4 KK514 pKa = 11.46 NSLDD518 pKa = 3.79 LVVDD522 pKa = 3.33 QHH524 pKa = 7.11 LDD526 pKa = 3.17 PGGYY530 pKa = 9.11 QVVFNWAEE538 pKa = 3.84 AGKK541 pKa = 10.53 EE542 pKa = 4.0 PTTSSALQFSVTEE555 pKa = 4.0 EE556 pKa = 4.03 PEE558 pKa = 4.39 YY559 pKa = 10.72 ISDD562 pKa = 3.74 VYY564 pKa = 11.36 GVVTIEE570 pKa = 4.22 KK571 pKa = 10.26 DD572 pKa = 3.62 GEE574 pKa = 4.02 DD575 pKa = 4.12 GYY577 pKa = 11.91 ALRR580 pKa = 11.84 LYY582 pKa = 10.74 DD583 pKa = 5.88 DD584 pKa = 3.62 EE585 pKa = 4.54 TSYY588 pKa = 11.68 DD589 pKa = 3.5 RR590 pKa = 11.84 SLTDD594 pKa = 3.37 MEE596 pKa = 4.68 KK597 pKa = 10.44 QNQVLLEE604 pKa = 3.85 FRR606 pKa = 11.84 GDD608 pKa = 3.38 FTAQYY613 pKa = 10.84 DD614 pKa = 3.7 EE615 pKa = 5.26 DD616 pKa = 4.88 GNLVSASAVSVEE628 pKa = 4.19 AADD631 pKa = 4.04 GTVSSTINISNCLDD645 pKa = 3.48 VEE647 pKa = 4.52 AGSLTIRR654 pKa = 11.84 VEE656 pKa = 4.15 NPGTEE661 pKa = 3.87 EE662 pKa = 3.83 QVINTDD668 pKa = 2.53 IDD670 pKa = 3.82 GKK672 pKa = 11.29 VYY674 pKa = 7.6 TTNSRR679 pKa = 11.84 TKK681 pKa = 9.56 VWNGVCAITSLEE693 pKa = 3.98 NGEE696 pKa = 4.71 EE697 pKa = 4.35 YY698 pKa = 10.32 PLLQYY703 pKa = 11.17 DD704 pKa = 3.93 YY705 pKa = 9.68 MGNPTSDD712 pKa = 3.5 VEE714 pKa = 4.23 NSVANTNAITLVWPGAASTAQTLAGILFEE743 pKa = 5.33 FRR745 pKa = 11.84 YY746 pKa = 10.1 CQFGLMALEE755 pKa = 4.67 TGTVTDD761 pKa = 3.43 QTPKK765 pKa = 10.22 KK766 pKa = 9.9 RR767 pKa = 11.84 VVAFGAEE774 pKa = 3.94 MSPDD778 pKa = 3.4 FLLPSNFDD786 pKa = 2.7 WGNRR790 pKa = 11.84 QTSTLEE796 pKa = 3.76 AVQLNLAKK804 pKa = 10.51 SNYY807 pKa = 8.99 TADD810 pKa = 3.41 QLRR813 pKa = 11.84 DD814 pKa = 3.41 VQARR818 pKa = 11.84 YY819 pKa = 8.97 AADD822 pKa = 3.36 QQAWEE827 pKa = 4.09 EE828 pKa = 4.44 AEE830 pKa = 4.16 TGSLSLYY837 pKa = 9.57 VHH839 pKa = 7.61 DD840 pKa = 4.79 ILFGGGFVGFNASVEE855 pKa = 4.36 VALPSYY861 pKa = 11.36 ADD863 pKa = 3.51 GLPGVEE869 pKa = 3.92 GTLNLRR875 pKa = 11.84 VMPDD879 pKa = 3.08 RR880 pKa = 11.84 DD881 pKa = 3.28 LWEE884 pKa = 4.11 VGVYY888 pKa = 10.43 GSADD892 pKa = 3.44 FAILNMEE899 pKa = 4.39 AEE901 pKa = 4.61 LVITAYY907 pKa = 11.25 NNIPLPNKK915 pKa = 9.25 IYY917 pKa = 10.12 FYY919 pKa = 11.3 VGGFTPGINVDD930 pKa = 3.14 GMGIFWIQGAGGGVEE945 pKa = 4.24 GLADD949 pKa = 3.75 TVFLSSRR956 pKa = 11.84 VPPLKK961 pKa = 10.69 LIFSGQFALFAVLQARR977 pKa = 11.84 ADD979 pKa = 3.55 LTMGMRR985 pKa = 11.84 EE986 pKa = 3.97 FSLGMRR992 pKa = 11.84 DD993 pKa = 3.81 LNIAGITLLEE1003 pKa = 4.44 SASLTTQWYY1012 pKa = 8.95 PEE1014 pKa = 3.93 LRR1016 pKa = 11.84 LNAGIEE1022 pKa = 4.08 LNILDD1027 pKa = 4.8 IIEE1030 pKa = 4.52 GSGSIVLEE1038 pKa = 3.5 EE1039 pKa = 4.38 DD1040 pKa = 2.87 KK1041 pKa = 11.28 RR1042 pKa = 11.84 EE1043 pKa = 3.91 NRR1045 pKa = 11.84 LFWEE1049 pKa = 5.37 GFATAGVTTPKK1060 pKa = 10.11 IPLIGEE1066 pKa = 3.7 IHH1068 pKa = 6.79 IGDD1071 pKa = 3.62 VDD1073 pKa = 4.45 LGLNASKK1080 pKa = 10.03 IWGALHH1086 pKa = 6.97 VLMLDD1091 pKa = 3.46 MGVTYY1096 pKa = 10.66 YY1097 pKa = 10.14 WGGDD1101 pKa = 2.88 VDD1103 pKa = 4.97 FAFGKK1108 pKa = 10.71 YY1109 pKa = 7.09 NTPEE1113 pKa = 3.9 ATVPAALSGIPVYY1126 pKa = 10.49 TDD1128 pKa = 2.7 AGTGRR1133 pKa = 11.84 VLYY1136 pKa = 9.94 MSVGTNARR1144 pKa = 11.84 LEE1146 pKa = 4.19 AATDD1150 pKa = 3.29 RR1151 pKa = 11.84 RR1152 pKa = 11.84 TFDD1155 pKa = 3.51 LAGTATRR1162 pKa = 11.84 TNGEE1166 pKa = 3.94 SSIDD1170 pKa = 3.46 RR1171 pKa = 11.84 SADD1174 pKa = 2.89 GMTHH1178 pKa = 6.57 VIQLGSYY1185 pKa = 9.66 SGASGKK1191 pKa = 10.42 ALTLSFQADD1200 pKa = 3.7 SLEE1203 pKa = 4.13 QAQRR1207 pKa = 11.84 IAMGDD1212 pKa = 3.26 SRR1214 pKa = 11.84 YY1215 pKa = 7.71 GTPGITLADD1224 pKa = 4.45 PDD1226 pKa = 5.34 DD1227 pKa = 4.86 PGQTYY1232 pKa = 8.95 SLRR1235 pKa = 11.84 WLDD1238 pKa = 3.5 TTQDD1242 pKa = 3.39 AEE1244 pKa = 4.32 EE1245 pKa = 4.13 QSDD1248 pKa = 3.93 ANALLSYY1255 pKa = 10.2 DD1256 pKa = 3.89 QEE1258 pKa = 4.44 SGEE1261 pKa = 4.34 ANVTISFTQAEE1272 pKa = 5.15 DD1273 pKa = 3.29 YY1274 pKa = 10.54 HH1275 pKa = 7.79 AWRR1278 pKa = 11.84 LTLGASAQSALYY1290 pKa = 10.24 DD1291 pKa = 3.43 IAYY1294 pKa = 10.33 LPGLDD1299 pKa = 3.55 SASVQWNDD1307 pKa = 3.02 QTQEE1311 pKa = 4.17 LTVTWQGSEE1320 pKa = 4.1 LNDD1323 pKa = 3.45 VEE1325 pKa = 4.64 NVAVYY1330 pKa = 10.04 AVSQTEE1336 pKa = 4.26 EE1337 pKa = 4.68 GEE1339 pKa = 4.46 STTTLLYY1346 pKa = 9.35 EE1347 pKa = 4.45 TDD1349 pKa = 3.43 DD1350 pKa = 3.49 TGIISGGVAGATQGSATFAVPTDD1373 pKa = 4.34 LPSGSYY1379 pKa = 9.39 TIQVVGSSEE1388 pKa = 4.01 TRR1390 pKa = 11.84 SVNDD1394 pKa = 3.19 VVKK1397 pKa = 10.98 VEE1399 pKa = 4.33 EE1400 pKa = 4.23 PLAYY1404 pKa = 9.32 TNPNQPQAPVIDD1416 pKa = 4.23 SVKK1419 pKa = 10.82 LGGDD1423 pKa = 3.29 YY1424 pKa = 10.95 SIDD1427 pKa = 3.5 VAVEE1431 pKa = 3.65 KK1432 pKa = 10.82 QGAYY1436 pKa = 10.02 DD1437 pKa = 4.29 GYY1439 pKa = 10.86 VATIYY1444 pKa = 10.24 EE1445 pKa = 4.2 QTDD1448 pKa = 3.35 TGLVATDD1455 pKa = 5.21 LAQQWVEE1462 pKa = 4.12 TSGEE1466 pKa = 4.11 APEE1469 pKa = 4.19 EE1470 pKa = 4.03 TLTLGGRR1477 pKa = 11.84 YY1478 pKa = 5.59 TTSAYY1483 pKa = 10.84 VDD1485 pKa = 3.49 GDD1487 pKa = 3.91 GNAVLPSEE1495 pKa = 4.63 AEE1497 pKa = 3.89 GRR1499 pKa = 11.84 DD1500 pKa = 3.4 DD1501 pKa = 3.62 VTSVEE1506 pKa = 4.05 QTVGVEE1512 pKa = 3.52 AGKK1515 pKa = 10.58 VYY1517 pKa = 10.71 KK1518 pKa = 10.25 IGLAGYY1524 pKa = 6.35 TTGPNGEE1531 pKa = 4.28 IFLSQEE1537 pKa = 4.32 TLSSAITMQAPQKK1550 pKa = 10.35 ATVTVQAEE1558 pKa = 4.64 GSFSVDD1564 pKa = 2.58 GTDD1567 pKa = 4.06 TINGVNATIQITSDD1581 pKa = 3.52 MPVSGTWTLDD1591 pKa = 2.84 GDD1593 pKa = 4.16 VASGQWQAGTDD1604 pKa = 3.88 QNFLDD1609 pKa = 3.7 QAIDD1613 pKa = 3.67 FFANLFGGEE1622 pKa = 4.1 RR1623 pKa = 11.84 EE1624 pKa = 4.35 QIGTIYY1630 pKa = 10.69 LSGRR1634 pKa = 11.84 AEE1636 pKa = 4.11 TDD1638 pKa = 3.26 VLPDD1642 pKa = 4.49 DD1643 pKa = 4.96 GGLTEE1648 pKa = 4.54 GTHH1651 pKa = 5.75 ILSLVGEE1658 pKa = 4.52 NEE1660 pKa = 3.75 QGDD1663 pKa = 4.42 AVSVKK1668 pKa = 10.67 YY1669 pKa = 8.45 MFRR1672 pKa = 11.84 VDD1674 pKa = 3.26 TTAPRR1679 pKa = 11.84 LQLISPADD1687 pKa = 3.37 GDD1689 pKa = 4.24 YY1690 pKa = 11.14 FSDD1693 pKa = 3.46 TVTVEE1698 pKa = 4.13 GLSEE1702 pKa = 4.29 PGAVVTVLVDD1712 pKa = 3.69 GQAAQTVTIPEE1723 pKa = 4.76 DD1724 pKa = 3.37 GTLSVEE1730 pKa = 4.01 IPVDD1734 pKa = 3.54 NTVLEE1739 pKa = 4.48 HH1740 pKa = 6.82 EE1741 pKa = 4.29 LTIYY1745 pKa = 11.09 AEE1747 pKa = 4.22 DD1748 pKa = 3.41 AVGNVGRR1755 pKa = 11.84 KK1756 pKa = 7.72 MSIPLLNDD1764 pKa = 3.13 IAAAPDD1770 pKa = 3.43 VALGILFNGQDD1781 pKa = 3.67 YY1782 pKa = 8.1 TQQNIPAGTNGVVQLCLVSGSRR1804 pKa = 11.84 RR1805 pKa = 11.84 VPVPADD1811 pKa = 3.04 SVMGRR1816 pKa = 11.84 QAEE1819 pKa = 4.22 WQTTVVKK1826 pKa = 9.63 GTASLDD1832 pKa = 3.47 NGLSLLTSLDD1842 pKa = 3.66 VNGMLTVSLDD1852 pKa = 3.45 QMQAAAVLGGNANDD1866 pKa = 5.11 IDD1868 pKa = 4.21 YY1869 pKa = 7.46 QTCNVTVTANEE1880 pKa = 4.31 GGTVSPAGTTAVTEE1894 pKa = 4.49 GEE1896 pKa = 4.58 SFTLTITPDD1905 pKa = 2.83 AYY1907 pKa = 10.31 YY1908 pKa = 9.92 HH1909 pKa = 5.96 VEE1911 pKa = 4.16 SVVVNN1916 pKa = 4.14
Molecular weight: 206.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.711
IPC2_protein 3.859
IPC_protein 3.884
Toseland 3.668
ProMoST 4.024
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.77
Rodwell 3.706
Grimsley 3.579
Solomon 3.859
Lehninger 3.821
Nozaki 3.961
DTASelect 4.19
Thurlkill 3.706
EMBOSS 3.783
Sillero 3.999
Patrickios 1.291
IPC_peptide 3.859
IPC2_peptide 3.986
IPC2.peptide.svr19 3.885
Protein with the highest isoelectric point:
>tr|A0A1Y4M6N6|A0A1Y4M6N6_9FIRM Uncharacterized protein OS=Pseudoflavonifractor sp. An176 OX=1965572 GN=B5F12_10555 PE=4 SV=1
MM1 pKa = 7.06 VRR3 pKa = 11.84 TYY5 pKa = 10.24 QPKK8 pKa = 9.12 KK9 pKa = 7.46 RR10 pKa = 11.84 HH11 pKa = 5.45 RR12 pKa = 11.84 SKK14 pKa = 10.49 VHH16 pKa = 5.66 GFRR19 pKa = 11.84 KK20 pKa = 10.08 RR21 pKa = 11.84 MATANGRR28 pKa = 11.84 KK29 pKa = 8.93 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.22 GRR39 pKa = 11.84 VRR41 pKa = 11.84 LTHH44 pKa = 6.42
Molecular weight: 5.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.433
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.384
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.106
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.067
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2212
0
2212
726739
37
2834
328.5
36.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.971 ± 0.06
1.562 ± 0.026
5.652 ± 0.053
6.593 ± 0.053
3.585 ± 0.036
7.8 ± 0.051
2.033 ± 0.024
5.189 ± 0.043
4.522 ± 0.055
9.955 ± 0.071
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.849 ± 0.023
3.457 ± 0.04
4.175 ± 0.031
4.452 ± 0.042
5.145 ± 0.055
5.475 ± 0.044
5.996 ± 0.066
7.809 ± 0.044
1.141 ± 0.017
3.638 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here