Wenzhou sobemo-like virus 2

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 5.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEF3|A0A1L3KEF3_9VIRU Uncharacterized protein OS=Wenzhou sobemo-like virus 2 OX=1923658 PE=4 SV=1
MM1 pKa = 7.43RR2 pKa = 11.84TRR4 pKa = 11.84IKK6 pKa = 10.39IKK8 pKa = 10.33SDD10 pKa = 2.81VNTFVRR16 pKa = 11.84EE17 pKa = 3.85EE18 pKa = 4.35FIRR21 pKa = 11.84ALQDD25 pKa = 2.92IDD27 pKa = 4.37PNSSPGCCEE36 pKa = 3.48MKK38 pKa = 10.76VYY40 pKa = 8.97GTTNRR45 pKa = 11.84EE46 pKa = 3.87CLGIQGGPEE55 pKa = 4.13GRR57 pKa = 11.84YY58 pKa = 7.56MDD60 pKa = 4.2PVRR63 pKa = 11.84VEE65 pKa = 3.37ALYY68 pKa = 11.1RR69 pKa = 11.84MVIGRR74 pKa = 11.84IQNLFEE80 pKa = 4.45GNYY83 pKa = 10.18VSDD86 pKa = 3.84NLLTFVKK93 pKa = 10.57DD94 pKa = 3.67EE95 pKa = 4.02PTKK98 pKa = 10.57LSKK101 pKa = 10.52IEE103 pKa = 3.96TKK105 pKa = 9.68RR106 pKa = 11.84WRR108 pKa = 11.84LISGVSLVDD117 pKa = 3.27TMVDD121 pKa = 3.83RR122 pKa = 11.84ILLQFLFEE130 pKa = 4.21QYY132 pKa = 9.38MDD134 pKa = 3.84RR135 pKa = 11.84LGTTPVAIGWNPYY148 pKa = 7.69TSAYY152 pKa = 9.92LFSIVMTPSEE162 pKa = 4.34PKK164 pKa = 9.78KK165 pKa = 10.43VHH167 pKa = 5.51PQYY170 pKa = 11.59VAMDD174 pKa = 4.04KK175 pKa = 11.04SCWDD179 pKa = 3.18WTVQTWLLDD188 pKa = 4.03AIMQVFIGAVVEE200 pKa = 4.43PPDD203 pKa = 2.82WWVYY207 pKa = 9.42LLKK210 pKa = 10.22TRR212 pKa = 11.84FYY214 pKa = 11.12CLFEE218 pKa = 5.44APTFEE223 pKa = 4.28FQDD226 pKa = 3.49GFTIKK231 pKa = 10.32QKK233 pKa = 10.81EE234 pKa = 4.03PGIMKK239 pKa = 9.8SGCYY243 pKa = 6.2MTLMANSIAQVLLDD257 pKa = 4.18SLCAQAAKK265 pKa = 10.1IDD267 pKa = 3.39VHH269 pKa = 7.28YY270 pKa = 9.98PFWCCGDD277 pKa = 3.88DD278 pKa = 4.12TIQMLPSDD286 pKa = 4.49FRR288 pKa = 11.84QLVDD292 pKa = 3.91YY293 pKa = 10.25INAMEE298 pKa = 4.58RR299 pKa = 11.84YY300 pKa = 8.93SYY302 pKa = 10.37NLKK305 pKa = 10.42VSYY308 pKa = 10.21HH309 pKa = 7.02DD310 pKa = 3.24EE311 pKa = 4.47AEE313 pKa = 4.3FVGSIMTRR321 pKa = 11.84KK322 pKa = 10.13GPVPAYY328 pKa = 8.74QAKK331 pKa = 9.93HH332 pKa = 5.3CWLLQRR338 pKa = 11.84LTLKK342 pKa = 10.82PEE344 pKa = 4.02DD345 pKa = 3.62QIATLRR351 pKa = 11.84SYY353 pKa = 11.25QIMYY357 pKa = 10.55ANEE360 pKa = 4.07PEE362 pKa = 4.31VLAKK366 pKa = 10.0IRR368 pKa = 11.84GLIDD372 pKa = 2.76KK373 pKa = 9.86WNIPEE378 pKa = 4.26AYY380 pKa = 7.94ITAEE384 pKa = 4.25EE385 pKa = 4.17LRR387 pKa = 11.84EE388 pKa = 4.02LQQSS392 pKa = 3.39

Molecular weight: 45.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEF3|A0A1L3KEF3_9VIRU Uncharacterized protein OS=Wenzhou sobemo-like virus 2 OX=1923658 PE=4 SV=1
MM1 pKa = 7.0STVAPDD7 pKa = 3.55GTFMRR12 pKa = 11.84NEE14 pKa = 3.99AYY16 pKa = 10.75VPGSEE21 pKa = 4.22YY22 pKa = 10.56QRR24 pKa = 11.84TVDD27 pKa = 5.31GIPKK31 pKa = 8.31FQCQITDD38 pKa = 3.21KK39 pKa = 10.76FGSSVLGYY47 pKa = 8.43ATWVGDD53 pKa = 3.94YY54 pKa = 10.39IVTPTHH60 pKa = 6.11VVATANTDD68 pKa = 3.17HH69 pKa = 6.89VLLLTRR75 pKa = 11.84NGAVSVPTDD84 pKa = 3.04QWRR87 pKa = 11.84HH88 pKa = 4.93LEE90 pKa = 4.13YY91 pKa = 11.03ADD93 pKa = 4.24VSYY96 pKa = 11.1LPYY99 pKa = 10.19EE100 pKa = 3.9QRR102 pKa = 11.84TYY104 pKa = 11.68SPLQLSKK111 pKa = 10.62GTVYY115 pKa = 10.8PPGKK119 pKa = 9.57EE120 pKa = 3.99VNNLFVTATTSTVFTTGIIRR140 pKa = 11.84EE141 pKa = 4.38HH142 pKa = 6.47PTDD145 pKa = 3.61ALYY148 pKa = 9.05VTYY151 pKa = 10.31EE152 pKa = 4.15GSTKK156 pKa = 10.25PGFSGVPYY164 pKa = 10.31VSNKK168 pKa = 9.52KK169 pKa = 9.74AVAMHH174 pKa = 6.89LGSNSYY180 pKa = 10.99GKK182 pKa = 10.25GIKK185 pKa = 9.98YY186 pKa = 9.97SWILIMIYY194 pKa = 9.68EE195 pKa = 4.5AEE197 pKa = 4.18NPNQNVRR204 pKa = 11.84HH205 pKa = 5.64YY206 pKa = 11.0SDD208 pKa = 3.18EE209 pKa = 4.24TEE211 pKa = 3.81KK212 pKa = 11.16VFIRR216 pKa = 11.84EE217 pKa = 4.22STGSDD222 pKa = 2.97TYY224 pKa = 11.52EE225 pKa = 4.03KK226 pKa = 10.72LIAQALEE233 pKa = 3.96RR234 pKa = 11.84MSQGEE239 pKa = 4.5RR240 pKa = 11.84YY241 pKa = 9.16FKK243 pKa = 10.18RR244 pKa = 11.84AGYY247 pKa = 10.23RR248 pKa = 11.84GMDD251 pKa = 2.85SYY253 pKa = 11.84LFNNSDD259 pKa = 3.18GTMMEE264 pKa = 4.28ITPHH268 pKa = 5.32TRR270 pKa = 11.84QHH272 pKa = 4.67MMAFNEE278 pKa = 4.01YY279 pKa = 10.92DD280 pKa = 3.27NDD282 pKa = 4.04AEE284 pKa = 4.31MMSGGYY290 pKa = 8.66RR291 pKa = 11.84RR292 pKa = 11.84KK293 pKa = 9.88RR294 pKa = 11.84SRR296 pKa = 11.84KK297 pKa = 9.12APSGWGEE304 pKa = 4.14LEE306 pKa = 3.6EE307 pKa = 4.21LAPVRR312 pKa = 11.84KK313 pKa = 10.06GKK315 pKa = 10.92GKK317 pKa = 10.39GGKK320 pKa = 8.6FRR322 pKa = 11.84YY323 pKa = 9.69DD324 pKa = 3.24EE325 pKa = 4.5PPAFKK330 pKa = 10.58GKK332 pKa = 9.89SWVDD336 pKa = 3.13MMEE339 pKa = 3.92EE340 pKa = 3.54SDD342 pKa = 4.24YY343 pKa = 11.68EE344 pKa = 4.17EE345 pKa = 4.94EE346 pKa = 4.72NADD349 pKa = 5.67DD350 pKa = 4.45SNQDD354 pKa = 3.02QPMVSQVKK362 pKa = 9.63NKK364 pKa = 10.21KK365 pKa = 9.7AIKK368 pKa = 10.21KK369 pKa = 10.06LGLDD373 pKa = 3.77NVPQTAMLVPDD384 pKa = 4.11ISRR387 pKa = 11.84PTVPVYY393 pKa = 10.75SDD395 pKa = 3.41YY396 pKa = 11.54PEE398 pKa = 4.03VVKK401 pKa = 10.09TSKK404 pKa = 10.83NVVSPPDD411 pKa = 3.25VEE413 pKa = 5.55APGKK417 pKa = 7.45TQTAEE422 pKa = 3.89EE423 pKa = 4.31VVIPLRR429 pKa = 11.84TTTGSLPTPNFNLDD443 pKa = 3.57HH444 pKa = 7.06LSNTAAPPLTLAQLGEE460 pKa = 4.38ALPLILEE467 pKa = 4.46SLNSLSNRR475 pKa = 11.84LQNPRR480 pKa = 11.84RR481 pKa = 11.84RR482 pKa = 11.84RR483 pKa = 11.84SRR485 pKa = 11.84KK486 pKa = 9.17PSAKK490 pKa = 9.65PSNSCAQEE498 pKa = 3.64

Molecular weight: 55.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

890

392

498

445.0

50.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.955 ± 0.222

1.348 ± 0.779

5.393 ± 0.307

6.629 ± 0.163

3.258 ± 0.533

5.955 ± 0.718

1.461 ± 0.285

4.831 ± 1.332

5.73 ± 0.242

7.64 ± 0.834

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.596 ± 0.315

4.27 ± 0.783

6.404 ± 0.678

4.27 ± 0.539

5.393 ± 0.023

6.854 ± 1.135

6.966 ± 0.712

7.191 ± 0.196

1.685 ± 0.561

5.169 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski