Pepper yellow leaf curl virus PSSWS-14
Average proteome isoelectric point is 7.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A140HM83|A0A140HM83_9GEMI Transcriptional activator protein OS=Pepper yellow leaf curl virus PSSWS-14 OX=1828512 GN=C2 PE=3 SV=1
MM1 pKa = 7.17 PRR3 pKa = 11.84 SYY5 pKa = 11.18 SFQVKK10 pKa = 9.86 AKK12 pKa = 10.29 NIFLTYY18 pKa = 8.78 PKK20 pKa = 10.43 CPIPKK25 pKa = 10.03 EE26 pKa = 3.92 EE27 pKa = 4.5 ALEE30 pKa = 3.85 LLKK33 pKa = 10.95 NIQCPSDD40 pKa = 3.23 KK41 pKa = 10.97 LFIRR45 pKa = 11.84 VAQEE49 pKa = 3.58 KK50 pKa = 10.5 HH51 pKa = 6.25 SDD53 pKa = 3.62 GSLHH57 pKa = 6.19 LHH59 pKa = 5.99 VLIQFKK65 pKa = 10.85 GKK67 pKa = 10.0 AQFRR71 pKa = 11.84 NNRR74 pKa = 11.84 HH75 pKa = 6.05 FDD77 pKa = 3.32 LTHH80 pKa = 7.22 PNTSTQFHH88 pKa = 7.33 PNFQGAKK95 pKa = 9.08 SSSDD99 pKa = 3.06 VKK101 pKa = 11.23 SYY103 pKa = 10.8 IEE105 pKa = 4.11 KK106 pKa = 10.91 DD107 pKa = 2.87 GDD109 pKa = 3.93 YY110 pKa = 11.1 VDD112 pKa = 3.8 WGVFQIDD119 pKa = 3.23 GRR121 pKa = 11.84 SARR124 pKa = 11.84 GGQQTANDD132 pKa = 3.91 AAAEE136 pKa = 3.92 ALNAGSKK143 pKa = 8.32 QAAMAIIRR151 pKa = 11.84 EE152 pKa = 4.31 KK153 pKa = 10.9 LPKK156 pKa = 9.83 EE157 pKa = 4.29 YY158 pKa = 10.15 IFQFHH163 pKa = 6.72 NLNANLDD170 pKa = 4.25 RR171 pKa = 11.84 IFAPPLEE178 pKa = 4.4 VFVCPFSSSSFDD190 pKa = 3.42 QVPEE194 pKa = 4.01 EE195 pKa = 4.01 LQAWAAEE202 pKa = 4.17 NVRR205 pKa = 11.84 DD206 pKa = 3.66 AAARR210 pKa = 11.84 PWRR213 pKa = 11.84 PNSIVIEE220 pKa = 4.38 GEE222 pKa = 3.92 SRR224 pKa = 11.84 TGKK227 pKa = 8.58 TMWARR232 pKa = 11.84 SLGLHH237 pKa = 6.59 NYY239 pKa = 10.03 LCGHH243 pKa = 7.38 LDD245 pKa = 4.39 LSPKK249 pKa = 10.12 VYY251 pKa = 11.11 NNDD254 pKa = 2.08 AWYY257 pKa = 10.88 NVIDD261 pKa = 5.2 DD262 pKa = 4.41 VDD264 pKa = 3.61 PHH266 pKa = 5.85 YY267 pKa = 11.0 LKK269 pKa = 10.7 HH270 pKa = 6.2 FKK272 pKa = 10.7 EE273 pKa = 4.4 FMGAQRR279 pKa = 11.84 NWQSNTKK286 pKa = 9.02 YY287 pKa = 10.63 GKK289 pKa = 9.49 PIQIKK294 pKa = 10.42 GGIPTIFLCNPGPTSSYY311 pKa = 11.06 KK312 pKa = 10.58 EE313 pKa = 3.96 YY314 pKa = 10.96 LDD316 pKa = 3.65 EE317 pKa = 5.84 DD318 pKa = 4.03 KK319 pKa = 11.74 NNALKK324 pKa = 10.54 SWALKK329 pKa = 10.01 NATFVTINGPLYY341 pKa = 10.77 SSSTEE346 pKa = 3.83 DD347 pKa = 3.08 TAPNCEE353 pKa = 4.37 EE354 pKa = 4.25 EE355 pKa = 4.35 NNPPEE360 pKa = 4.37 TYY362 pKa = 10.87
Molecular weight: 40.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.204
IPC2_protein 6.173
IPC_protein 6.287
Toseland 6.59
ProMoST 6.722
Dawson 6.649
Bjellqvist 6.605
Wikipedia 6.664
Rodwell 6.634
Grimsley 6.722
Solomon 6.649
Lehninger 6.649
Nozaki 6.912
DTASelect 7.088
Thurlkill 7.117
EMBOSS 7.088
Sillero 7.029
Patrickios 4.177
IPC_peptide 6.664
IPC2_peptide 6.825
IPC2.peptide.svr19 6.826
Protein with the highest isoelectric point:
>tr|A0A140HM82|A0A140HM82_9GEMI Replication-associated protein OS=Pepper yellow leaf curl virus PSSWS-14 OX=1828512 GN=C1 PE=3 SV=1
MM1 pKa = 7.94 PKK3 pKa = 10.24 RR4 pKa = 11.84 SIDD7 pKa = 3.85 TVSSLPMSITRR18 pKa = 11.84 RR19 pKa = 11.84 RR20 pKa = 11.84 LNYY23 pKa = 9.63 ASQYY27 pKa = 9.85 SLPAAAPTAPGMSYY41 pKa = 10.55 KK42 pKa = 10.11 RR43 pKa = 11.84 RR44 pKa = 11.84 AWVNRR49 pKa = 11.84 PMNRR53 pKa = 11.84 KK54 pKa = 7.88 PRR56 pKa = 11.84 FYY58 pKa = 10.48 RR59 pKa = 11.84 GRR61 pKa = 11.84 RR62 pKa = 11.84 TSDD65 pKa = 3.14 VPRR68 pKa = 11.84 GCEE71 pKa = 4.29 GPCKK75 pKa = 10.21 VQSFEE80 pKa = 3.81 QRR82 pKa = 11.84 HH83 pKa = 5.68 DD84 pKa = 3.43 VTHH87 pKa = 6.3 TGKK90 pKa = 10.02 VLCVSDD96 pKa = 3.48 VTRR99 pKa = 11.84 GGGITHH105 pKa = 7.09 RR106 pKa = 11.84 VGKK109 pKa = 9.44 RR110 pKa = 11.84 FCVKK114 pKa = 9.73 SVYY117 pKa = 10.25 IIGKK121 pKa = 8.61 VWMDD125 pKa = 3.44 EE126 pKa = 4.13 NIKK129 pKa = 10.76 SKK131 pKa = 10.6 NHH133 pKa = 5.55 TNNVMFWLVRR143 pKa = 11.84 DD144 pKa = 3.99 RR145 pKa = 11.84 RR146 pKa = 11.84 PVTTPYY152 pKa = 11.31 GFGEE156 pKa = 4.7 LFNMYY161 pKa = 10.54 DD162 pKa = 4.09 NEE164 pKa = 4.4 PSTATIKK171 pKa = 10.86 NDD173 pKa = 2.9 LRR175 pKa = 11.84 DD176 pKa = 3.56 RR177 pKa = 11.84 VQVLHH182 pKa = 6.82 RR183 pKa = 11.84 FSATVTGGQYY193 pKa = 10.83 ASKK196 pKa = 9.76 EE197 pKa = 3.75 QAIVKK202 pKa = 10.16 RR203 pKa = 11.84 FFRR206 pKa = 11.84 VNNYY210 pKa = 7.88 VVYY213 pKa = 10.46 NHH215 pKa = 6.03 QEE217 pKa = 3.42 AAKK220 pKa = 10.4 YY221 pKa = 9.25 EE222 pKa = 4.13 NHH224 pKa = 6.46 TEE226 pKa = 3.99 NALLLYY232 pKa = 7.29 MACTHH237 pKa = 7.07 ASNPVYY243 pKa = 9.86 ATLKK247 pKa = 9.47 IRR249 pKa = 11.84 IYY251 pKa = 10.67 FYY253 pKa = 11.5 DD254 pKa = 3.33 NVTNN258 pKa = 4.07
Molecular weight: 29.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.296
IPC2_protein 9.633
IPC_protein 10.058
Toseland 10.087
ProMoST 9.882
Dawson 10.335
Bjellqvist 10.058
Wikipedia 10.54
Rodwell 10.584
Grimsley 10.423
Solomon 10.365
Lehninger 10.321
Nozaki 10.116
DTASelect 10.043
Thurlkill 10.175
EMBOSS 10.511
Sillero 10.248
Patrickios 9.897
IPC_peptide 10.35
IPC2_peptide 9.033
IPC2.peptide.svr19 8.535
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1006
117
362
201.2
23.08
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.362 ± 0.867
2.286 ± 0.661
4.274 ± 0.538
4.771 ± 0.666
4.672 ± 0.38
5.268 ± 0.417
3.976 ± 0.71
5.169 ± 0.804
6.064 ± 0.501
7.256 ± 0.864
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.69 ± 0.428
6.163 ± 1.082
5.567 ± 0.651
4.771 ± 0.772
6.362 ± 1.266
7.356 ± 1.185
4.97 ± 0.752
6.66 ± 1.301
1.59 ± 0.31
4.771 ± 0.576
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here