Pepper yellow leaf curl virus PSSWS-14

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus; Pepper yellow leaf curl Indonesia virus 2

Average proteome isoelectric point is 7.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A140HM83|A0A140HM83_9GEMI Transcriptional activator protein OS=Pepper yellow leaf curl virus PSSWS-14 OX=1828512 GN=C2 PE=3 SV=1
MM1 pKa = 7.17PRR3 pKa = 11.84SYY5 pKa = 11.18SFQVKK10 pKa = 9.86AKK12 pKa = 10.29NIFLTYY18 pKa = 8.78PKK20 pKa = 10.43CPIPKK25 pKa = 10.03EE26 pKa = 3.92EE27 pKa = 4.5ALEE30 pKa = 3.85LLKK33 pKa = 10.95NIQCPSDD40 pKa = 3.23KK41 pKa = 10.97LFIRR45 pKa = 11.84VAQEE49 pKa = 3.58KK50 pKa = 10.5HH51 pKa = 6.25SDD53 pKa = 3.62GSLHH57 pKa = 6.19LHH59 pKa = 5.99VLIQFKK65 pKa = 10.85GKK67 pKa = 10.0AQFRR71 pKa = 11.84NNRR74 pKa = 11.84HH75 pKa = 6.05FDD77 pKa = 3.32LTHH80 pKa = 7.22PNTSTQFHH88 pKa = 7.33PNFQGAKK95 pKa = 9.08SSSDD99 pKa = 3.06VKK101 pKa = 11.23SYY103 pKa = 10.8IEE105 pKa = 4.11KK106 pKa = 10.91DD107 pKa = 2.87GDD109 pKa = 3.93YY110 pKa = 11.1VDD112 pKa = 3.8WGVFQIDD119 pKa = 3.23GRR121 pKa = 11.84SARR124 pKa = 11.84GGQQTANDD132 pKa = 3.91AAAEE136 pKa = 3.92ALNAGSKK143 pKa = 8.32QAAMAIIRR151 pKa = 11.84EE152 pKa = 4.31KK153 pKa = 10.9LPKK156 pKa = 9.83EE157 pKa = 4.29YY158 pKa = 10.15IFQFHH163 pKa = 6.72NLNANLDD170 pKa = 4.25RR171 pKa = 11.84IFAPPLEE178 pKa = 4.4VFVCPFSSSSFDD190 pKa = 3.42QVPEE194 pKa = 4.01EE195 pKa = 4.01LQAWAAEE202 pKa = 4.17NVRR205 pKa = 11.84DD206 pKa = 3.66AAARR210 pKa = 11.84PWRR213 pKa = 11.84PNSIVIEE220 pKa = 4.38GEE222 pKa = 3.92SRR224 pKa = 11.84TGKK227 pKa = 8.58TMWARR232 pKa = 11.84SLGLHH237 pKa = 6.59NYY239 pKa = 10.03LCGHH243 pKa = 7.38LDD245 pKa = 4.39LSPKK249 pKa = 10.12VYY251 pKa = 11.11NNDD254 pKa = 2.08AWYY257 pKa = 10.88NVIDD261 pKa = 5.2DD262 pKa = 4.41VDD264 pKa = 3.61PHH266 pKa = 5.85YY267 pKa = 11.0LKK269 pKa = 10.7HH270 pKa = 6.2FKK272 pKa = 10.7EE273 pKa = 4.4FMGAQRR279 pKa = 11.84NWQSNTKK286 pKa = 9.02YY287 pKa = 10.63GKK289 pKa = 9.49PIQIKK294 pKa = 10.42GGIPTIFLCNPGPTSSYY311 pKa = 11.06KK312 pKa = 10.58EE313 pKa = 3.96YY314 pKa = 10.96LDD316 pKa = 3.65EE317 pKa = 5.84DD318 pKa = 4.03KK319 pKa = 11.74NNALKK324 pKa = 10.54SWALKK329 pKa = 10.01NATFVTINGPLYY341 pKa = 10.77SSSTEE346 pKa = 3.83DD347 pKa = 3.08TAPNCEE353 pKa = 4.37EE354 pKa = 4.25EE355 pKa = 4.35NNPPEE360 pKa = 4.37TYY362 pKa = 10.87

Molecular weight: 40.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A140HM82|A0A140HM82_9GEMI Replication-associated protein OS=Pepper yellow leaf curl virus PSSWS-14 OX=1828512 GN=C1 PE=3 SV=1
MM1 pKa = 7.94PKK3 pKa = 10.24RR4 pKa = 11.84SIDD7 pKa = 3.85TVSSLPMSITRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNYY23 pKa = 9.63ASQYY27 pKa = 9.85SLPAAAPTAPGMSYY41 pKa = 10.55KK42 pKa = 10.11RR43 pKa = 11.84RR44 pKa = 11.84AWVNRR49 pKa = 11.84PMNRR53 pKa = 11.84KK54 pKa = 7.88PRR56 pKa = 11.84FYY58 pKa = 10.48RR59 pKa = 11.84GRR61 pKa = 11.84RR62 pKa = 11.84TSDD65 pKa = 3.14VPRR68 pKa = 11.84GCEE71 pKa = 4.29GPCKK75 pKa = 10.21VQSFEE80 pKa = 3.81QRR82 pKa = 11.84HH83 pKa = 5.68DD84 pKa = 3.43VTHH87 pKa = 6.3TGKK90 pKa = 10.02VLCVSDD96 pKa = 3.48VTRR99 pKa = 11.84GGGITHH105 pKa = 7.09RR106 pKa = 11.84VGKK109 pKa = 9.44RR110 pKa = 11.84FCVKK114 pKa = 9.73SVYY117 pKa = 10.25IIGKK121 pKa = 8.61VWMDD125 pKa = 3.44EE126 pKa = 4.13NIKK129 pKa = 10.76SKK131 pKa = 10.6NHH133 pKa = 5.55TNNVMFWLVRR143 pKa = 11.84DD144 pKa = 3.99RR145 pKa = 11.84RR146 pKa = 11.84PVTTPYY152 pKa = 11.31GFGEE156 pKa = 4.7LFNMYY161 pKa = 10.54DD162 pKa = 4.09NEE164 pKa = 4.4PSTATIKK171 pKa = 10.86NDD173 pKa = 2.9LRR175 pKa = 11.84DD176 pKa = 3.56RR177 pKa = 11.84VQVLHH182 pKa = 6.82RR183 pKa = 11.84FSATVTGGQYY193 pKa = 10.83ASKK196 pKa = 9.76EE197 pKa = 3.75QAIVKK202 pKa = 10.16RR203 pKa = 11.84FFRR206 pKa = 11.84VNNYY210 pKa = 7.88VVYY213 pKa = 10.46NHH215 pKa = 6.03QEE217 pKa = 3.42AAKK220 pKa = 10.4YY221 pKa = 9.25EE222 pKa = 4.13NHH224 pKa = 6.46TEE226 pKa = 3.99NALLLYY232 pKa = 7.29MACTHH237 pKa = 7.07ASNPVYY243 pKa = 9.86ATLKK247 pKa = 9.47IRR249 pKa = 11.84IYY251 pKa = 10.67FYY253 pKa = 11.5DD254 pKa = 3.33NVTNN258 pKa = 4.07

Molecular weight: 29.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1006

117

362

201.2

23.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.362 ± 0.867

2.286 ± 0.661

4.274 ± 0.538

4.771 ± 0.666

4.672 ± 0.38

5.268 ± 0.417

3.976 ± 0.71

5.169 ± 0.804

6.064 ± 0.501

7.256 ± 0.864

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.69 ± 0.428

6.163 ± 1.082

5.567 ± 0.651

4.771 ± 0.772

6.362 ± 1.266

7.356 ± 1.185

4.97 ± 0.752

6.66 ± 1.301

1.59 ± 0.31

4.771 ± 0.576
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski