Mycolicibacterium vanbaalenii (Mycobacterium vanbaalenii)
Average proteome isoelectric point is 5.91
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6378 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5S9MZ60|A0A5S9MZ60_MYCVN Uric acid degradation bifunctional protein OS=Mycolicibacterium vanbaalenii OX=110539 GN=uao_2 PE=4 SV=1
MM1 pKa = 7.53 RR2 pKa = 11.84 VFAASLILATPFALSACGSNDD23 pKa = 2.77 EE24 pKa = 4.28 TAAPSGTSPAADD36 pKa = 3.98 GEE38 pKa = 4.81 CPATPVDD45 pKa = 3.81 VVVSVDD51 pKa = 3.04 QWGDD55 pKa = 3.03 IVSQLGGACANVTTVLASSSVDD77 pKa = 3.12 PHH79 pKa = 8.5 DD80 pKa = 4.55 YY81 pKa = 11.01 EE82 pKa = 4.77 PAPSDD87 pKa = 3.18 AALFDD92 pKa = 3.88 GAQLVVINGGHH103 pKa = 5.48 YY104 pKa = 10.46 DD105 pKa = 3.18 EE106 pKa = 5.19 WAAKK110 pKa = 9.31 LAAASAPDD118 pKa = 3.82 APIVNAVEE126 pKa = 4.35 SSGGHH131 pKa = 6.1 ADD133 pKa = 3.99 EE134 pKa = 5.92 AGHH137 pKa = 6.64 DD138 pKa = 3.95 HH139 pKa = 7.85 DD140 pKa = 6.06 GGEE143 pKa = 4.3 ADD145 pKa = 4.44 HH146 pKa = 7.37 DD147 pKa = 3.54 QDD149 pKa = 3.81 QDD151 pKa = 3.39 HH152 pKa = 7.19 DD153 pKa = 4.28 HH154 pKa = 7.13 DD155 pKa = 4.65 HH156 pKa = 7.09 ADD158 pKa = 3.79 EE159 pKa = 5.29 AGHH162 pKa = 6.48 DD163 pKa = 3.83 HH164 pKa = 7.05 AGEE167 pKa = 4.54 GNPHH171 pKa = 4.99 VWYY174 pKa = 10.09 SPSVVTSVAEE184 pKa = 3.97 AVTGEE189 pKa = 4.12 LSEE192 pKa = 4.68 LAPDD196 pKa = 3.33 AAGYY200 pKa = 10.26 FDD202 pKa = 4.06 EE203 pKa = 4.89 RR204 pKa = 11.84 HH205 pKa = 4.92 TAFEE209 pKa = 4.31 NVMTPYY215 pKa = 10.41 YY216 pKa = 9.86 DD217 pKa = 3.74 TIAAIKK223 pKa = 9.65 ADD225 pKa = 3.77 SVGKK229 pKa = 10.46 SFAATEE235 pKa = 4.4 SVFDD239 pKa = 4.97 DD240 pKa = 3.32 MGAALGLQNRR250 pKa = 11.84 TPEE253 pKa = 4.79 GYY255 pKa = 10.07 QLASSNEE262 pKa = 4.01 AEE264 pKa = 4.63 PAPADD269 pKa = 3.42 LDD271 pKa = 3.66 AFLRR275 pKa = 11.84 LLGDD279 pKa = 3.36 RR280 pKa = 11.84 GVDD283 pKa = 3.18 VLIYY287 pKa = 8.68 NTQTEE292 pKa = 4.55 GSVPEE297 pKa = 4.21 QIRR300 pKa = 11.84 AAAEE304 pKa = 3.8 QAGVPVVEE312 pKa = 4.67 VTEE315 pKa = 4.27 TVPPNTEE322 pKa = 4.42 SFQTWQVAQLDD333 pKa = 4.29 SLAEE337 pKa = 3.94 ALGVPVV343 pKa = 4.15
Molecular weight: 35.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.704
IPC2_protein 3.846
IPC_protein 3.859
Toseland 3.656
ProMoST 4.012
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.77
Rodwell 3.694
Grimsley 3.567
Solomon 3.846
Lehninger 3.795
Nozaki 3.961
DTASelect 4.19
Thurlkill 3.694
EMBOSS 3.783
Sillero 3.986
Patrickios 1.329
IPC_peptide 3.846
IPC2_peptide 3.961
IPC2.peptide.svr19 3.866
Protein with the highest isoelectric point:
>tr|A0A5S9PXT8|A0A5S9PXT8_MYCVN Uncharacterized protein OS=Mycolicibacterium vanbaalenii OX=110539 GN=AELLOGFF_00726 PE=4 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.38 KK7 pKa = 8.42 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.06 RR11 pKa = 11.84 MSKK14 pKa = 9.76 KK15 pKa = 9.54 KK16 pKa = 9.72 HH17 pKa = 5.63 RR18 pKa = 11.84 KK19 pKa = 7.56 LLRR22 pKa = 11.84 RR23 pKa = 11.84 TRR25 pKa = 11.84 VQRR28 pKa = 11.84 RR29 pKa = 11.84 KK30 pKa = 10.11 LGKK33 pKa = 9.87
Molecular weight: 4.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.507
IPC2_protein 11.184
IPC_protein 12.749
Toseland 12.925
ProMoST 13.408
Dawson 12.925
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.735
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.457
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.111
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6378
0
6378
2015758
29
3732
316.0
33.94
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.849 ± 0.04
0.825 ± 0.01
6.447 ± 0.026
5.383 ± 0.028
3.059 ± 0.019
8.851 ± 0.03
2.184 ± 0.016
4.202 ± 0.019
2.072 ± 0.019
9.738 ± 0.031
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.12 ± 0.013
2.202 ± 0.013
5.795 ± 0.024
3.013 ± 0.017
7.21 ± 0.028
5.55 ± 0.021
6.12 ± 0.024
8.769 ± 0.027
1.514 ± 0.013
2.092 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here