Dermatophagoides pteronyssinus (European house dust mite)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Chelicerata; Arachnida; Acari; Acariformes; Sarcoptiformes; Astigmata; Psoroptidia; Analgoidea; Pyroglyphidae; Dermatophagoidinae; Dermatophagoides

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12480 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6P6Y1C1|A0A6P6Y1C1_DERPT eIF-2B GDP-GTP exchange factor subunit alpha OS=Dermatophagoides pteronyssinus OX=6956 GN=LOC113792416 PE=3 SV=1
MM1 pKa = 7.76SDD3 pKa = 3.99SDD5 pKa = 5.01DD6 pKa = 4.79FSDD9 pKa = 5.28DD10 pKa = 3.43DD11 pKa = 5.2HH12 pKa = 9.57SFDD15 pKa = 4.38SHH17 pKa = 7.21NDD19 pKa = 3.15SLDD22 pKa = 3.94GYY24 pKa = 11.13DD25 pKa = 5.56SDD27 pKa = 5.97DD28 pKa = 4.33FSNDD32 pKa = 2.41DD33 pKa = 3.64HH34 pKa = 7.07TFDD37 pKa = 4.22SHH39 pKa = 7.07NNSLDD44 pKa = 3.21GYY46 pKa = 10.04YY47 pKa = 10.76DD48 pKa = 3.9SDD50 pKa = 3.9VLNCSDD56 pKa = 3.92VDD58 pKa = 3.87DD59 pKa = 5.85ANFSDD64 pKa = 5.14DD65 pKa = 4.0HH66 pKa = 8.79ASDD69 pKa = 4.96DD70 pKa = 4.11LLFDD74 pKa = 3.94EE75 pKa = 5.02EE76 pKa = 4.97QNYY79 pKa = 10.44SDD81 pKa = 3.36NDD83 pKa = 3.42NRR85 pKa = 11.84IEE87 pKa = 4.33EE88 pKa = 4.22LDD90 pKa = 3.75EE91 pKa = 5.0NNEE94 pKa = 4.02QQHH97 pKa = 6.48LEE99 pKa = 3.98NEE101 pKa = 4.59MINDD105 pKa = 3.73DD106 pKa = 3.85TSSEE110 pKa = 4.12EE111 pKa = 3.65IEE113 pKa = 4.37EE114 pKa = 4.22NSNHH118 pKa = 5.95SVISDD123 pKa = 3.61DD124 pKa = 4.67DD125 pKa = 4.06NEE127 pKa = 4.41EE128 pKa = 3.97NVLDD132 pKa = 4.51DD133 pKa = 5.31NISEE137 pKa = 4.4YY138 pKa = 11.34VPDD141 pKa = 4.0TDD143 pKa = 6.4DD144 pKa = 5.54YY145 pKa = 12.1DD146 pKa = 6.73DD147 pKa = 6.7DD148 pKa = 7.47DD149 pKa = 7.49DD150 pKa = 7.6DD151 pKa = 7.71DD152 pKa = 7.3DD153 pKa = 6.78DD154 pKa = 6.14DD155 pKa = 6.2SSNQLYY161 pKa = 10.24TNFLMSLDD169 pKa = 3.88HH170 pKa = 5.56QTDD173 pKa = 3.7YY174 pKa = 11.36NGPVAVDD181 pKa = 3.28QQSNDD186 pKa = 3.15KK187 pKa = 10.51KK188 pKa = 11.07YY189 pKa = 11.07KK190 pKa = 9.06NQEE193 pKa = 3.89TQTDD197 pKa = 3.84VTNLIVQNRR206 pKa = 11.84CSICLEE212 pKa = 4.21SFCPNKK218 pKa = 10.3DD219 pKa = 3.26HH220 pKa = 7.39YY221 pKa = 10.64VVCLPCGHH229 pKa = 7.56LFGKK233 pKa = 8.09TCIEE237 pKa = 3.79KK238 pKa = 9.63WLASSKK244 pKa = 10.58YY245 pKa = 9.8CPTCRR250 pKa = 11.84FHH252 pKa = 8.96AEE254 pKa = 3.34IGDD257 pKa = 3.89IIKK260 pKa = 10.39IFLNPIDD267 pKa = 4.22INLMKK272 pKa = 10.46QNCEE276 pKa = 3.82TKK278 pKa = 9.64FWRR281 pKa = 11.84DD282 pKa = 2.79KK283 pKa = 9.01TTEE286 pKa = 3.87LTLEE290 pKa = 3.99NLQLKK295 pKa = 9.15MEE297 pKa = 4.56LHH299 pKa = 5.87LAKK302 pKa = 10.67NKK304 pKa = 10.08IINQIDD310 pKa = 3.85FNEE313 pKa = 4.36KK314 pKa = 10.12

Molecular weight: 36.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6P6Y7E7|A0A6P6Y7E7_DERPT DNA-directed RNA polymerase subunit OS=Dermatophagoides pteronyssinus OX=6956 GN=LOC113795351 PE=3 SV=1
RR1 pKa = 7.49LSRR4 pKa = 11.84RR5 pKa = 11.84LSRR8 pKa = 11.84RR9 pKa = 11.84GARR12 pKa = 11.84RR13 pKa = 11.84AAASRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84VRR22 pKa = 11.84AAGRR26 pKa = 11.84RR27 pKa = 11.84SRR29 pKa = 11.84TRR31 pKa = 11.84GSRR34 pKa = 11.84APPRR38 pKa = 11.84STRR41 pKa = 11.84RR42 pKa = 11.84AASTATTNRR51 pKa = 11.84ASRR54 pKa = 11.84SRR56 pKa = 11.84ARR58 pKa = 11.84ARR60 pKa = 11.84FCSSSSRR67 pKa = 11.84SRR69 pKa = 11.84FSSSSS74 pKa = 3.05

Molecular weight: 8.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11190

1290

12480

7101821

20

8940

569.1

64.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.491 ± 0.022

1.782 ± 0.017

5.838 ± 0.019

5.419 ± 0.026

4.312 ± 0.019

4.442 ± 0.023

2.875 ± 0.013

7.629 ± 0.023

6.176 ± 0.024

8.717 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.437 ± 0.008

7.584 ± 0.028

4.224 ± 0.022

5.449 ± 0.026

4.675 ± 0.014

9.331 ± 0.035

5.895 ± 0.025

4.402 ± 0.016

0.927 ± 0.007

3.386 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski