Pontibacillus yanchengensis Y32
Average proteome isoelectric point is 5.96
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3964 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A2TAL2|A0A0A2TAL2_9BACI Uncharacterized protein OS=Pontibacillus yanchengensis Y32 OX=1385514 GN=N782_11545 PE=4 SV=1
MM1 pKa = 7.59 ANILITFASMSGNTEE16 pKa = 3.58 EE17 pKa = 4.95 MADD20 pKa = 3.94 LLKK23 pKa = 10.95 DD24 pKa = 4.41 DD25 pKa = 4.46 IEE27 pKa = 4.56 KK28 pKa = 10.55 HH29 pKa = 4.2 GHH31 pKa = 5.04 EE32 pKa = 4.37 VTLEE36 pKa = 3.91 EE37 pKa = 4.56 LDD39 pKa = 3.82 EE40 pKa = 4.91 LSAHH44 pKa = 6.76 DD45 pKa = 3.95 LVHH48 pKa = 7.0 YY49 pKa = 10.12 EE50 pKa = 4.38 GILLGSYY57 pKa = 7.6 TWNDD61 pKa = 2.38 GDD63 pKa = 4.68 LPYY66 pKa = 10.47 EE67 pKa = 4.0 VEE69 pKa = 5.18 DD70 pKa = 3.55 IYY72 pKa = 11.59 EE73 pKa = 4.39 EE74 pKa = 4.67 IEE76 pKa = 4.49 DD77 pKa = 4.19 VDD79 pKa = 3.87 LTGIKK84 pKa = 10.12 VAVFGSGDD92 pKa = 3.43 TNYY95 pKa = 10.33 PLYY98 pKa = 10.77 CEE100 pKa = 4.47 AVHH103 pKa = 6.13 TFEE106 pKa = 5.28 KK107 pKa = 10.81 QLTSSGATLVQDD119 pKa = 3.58 GLKK122 pKa = 9.96 IEE124 pKa = 4.37 MAPDD128 pKa = 3.33 SDD130 pKa = 4.2 EE131 pKa = 5.55 DD132 pKa = 3.99 FDD134 pKa = 5.18 NISNFGDD141 pKa = 3.38 AFATFFNKK149 pKa = 9.84 TPVV152 pKa = 2.95
Molecular weight: 16.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.743
IPC2_protein 3.808
IPC_protein 3.795
Toseland 3.592
ProMoST 3.948
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.694
Rodwell 3.63
Grimsley 3.503
Solomon 3.77
Lehninger 3.719
Nozaki 3.884
DTASelect 4.088
Thurlkill 3.643
EMBOSS 3.706
Sillero 3.91
Patrickios 1.863
IPC_peptide 3.757
IPC2_peptide 3.897
IPC2.peptide.svr19 3.812
Protein with the highest isoelectric point:
>tr|A0A0A2TCG5|A0A0A2TCG5_9BACI Protein RecA OS=Pontibacillus yanchengensis Y32 OX=1385514 GN=recA PE=3 SV=1
MM1 pKa = 7.44 KK2 pKa = 9.6 RR3 pKa = 11.84 TFQPNNRR10 pKa = 11.84 KK11 pKa = 9.23 RR12 pKa = 11.84 KK13 pKa = 8.02 KK14 pKa = 8.47 VHH16 pKa = 5.57 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MSDD24 pKa = 2.85 ANGRR28 pKa = 11.84 QVLKK32 pKa = 10.29 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.96 GRR39 pKa = 11.84 KK40 pKa = 8.7 VLSAA44 pKa = 4.05
Molecular weight: 5.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.426
IPC2_protein 10.921
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.398
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.135
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.077
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3964
0
3964
1154180
37
1570
291.2
32.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.504 ± 0.038
0.633 ± 0.012
5.356 ± 0.035
7.903 ± 0.044
4.489 ± 0.031
6.722 ± 0.038
2.34 ± 0.021
7.389 ± 0.039
6.347 ± 0.032
9.435 ± 0.037
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.932 ± 0.017
4.456 ± 0.029
3.655 ± 0.026
4.356 ± 0.035
3.873 ± 0.024
6.231 ± 0.029
5.575 ± 0.022
7.011 ± 0.032
1.084 ± 0.015
3.707 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here