Flavobacterium fontis

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Flavobacterium

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2756 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M5C9R6|A0A1M5C9R6_9FLAO Uncharacterized protein OS=Flavobacterium fontis OX=1124188 GN=SAMN05444377_11113 PE=4 SV=1
MM1 pKa = 7.32FKK3 pKa = 10.74KK4 pKa = 10.7LLFFGILLTMMSCEE18 pKa = 4.16KK19 pKa = 10.63EE20 pKa = 4.31SLTEE24 pKa = 3.91TTSNSNLTQSSLLTGLLKK42 pKa = 10.62RR43 pKa = 11.84IAQNPTAFDD52 pKa = 3.69NVLDD56 pKa = 4.35GCSGFSVVLPVTVTVNGSTITVASEE81 pKa = 4.22SDD83 pKa = 3.5FQTVQNIKK91 pKa = 10.19NAYY94 pKa = 10.14SNDD97 pKa = 3.24TDD99 pKa = 3.77EE100 pKa = 5.72VFFTYY105 pKa = 9.9PIQLRR110 pKa = 11.84LKK112 pKa = 8.22NHH114 pKa = 5.58QLVTVTSYY122 pKa = 11.49NQFEE126 pKa = 4.79DD127 pKa = 4.35LYY129 pKa = 10.44EE130 pKa = 5.16DD131 pKa = 4.2YY132 pKa = 11.38SSDD135 pKa = 3.46DD136 pKa = 3.51LTEE139 pKa = 3.95IRR141 pKa = 11.84CVNIQFPITINTYY154 pKa = 8.11NTSSQQTQAYY164 pKa = 6.81TLTNDD169 pKa = 2.76VSLYY173 pKa = 10.62QFLASLTPNLQYY185 pKa = 11.18SLSYY189 pKa = 10.23PLTVTDD195 pKa = 5.36ANNNPQTITSNVGFEE210 pKa = 4.3SVIDD214 pKa = 4.27ANLSLCDD221 pKa = 3.67VPVGNTPLSFTTLLTDD237 pKa = 4.22NIWYY241 pKa = 9.14VSSYY245 pKa = 10.86IDD247 pKa = 5.47DD248 pKa = 5.24DD249 pKa = 5.43GSDD252 pKa = 3.59DD253 pKa = 3.6TDD255 pKa = 4.17DD256 pKa = 4.6YY257 pKa = 12.03EE258 pKa = 7.01DD259 pKa = 3.56YY260 pKa = 11.19TFDD263 pKa = 3.62FLSNFDD269 pKa = 3.33IQVFEE274 pKa = 4.59GSLFANGSWNYY285 pKa = 7.95YY286 pKa = 5.43TTGNTQKK293 pKa = 11.24LNLNFSNADD302 pKa = 4.16LLDD305 pKa = 4.8LDD307 pKa = 4.07QDD309 pKa = 3.78WTIVEE314 pKa = 4.14YY315 pKa = 9.87TIGVIRR321 pKa = 11.84LRR323 pKa = 11.84YY324 pKa = 8.89IDD326 pKa = 3.9SDD328 pKa = 3.8NDD330 pKa = 3.22VEE332 pKa = 4.43YY333 pKa = 11.03LVLRR337 pKa = 11.84KK338 pKa = 9.71LL339 pKa = 3.63

Molecular weight: 38.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M4VQD7|A0A1M4VQD7_9FLAO Peptidase family M1 OS=Flavobacterium fontis OX=1124188 GN=SAMN05444377_10117 PE=4 SV=1
MM1 pKa = 7.7EE2 pKa = 5.73NGTEE6 pKa = 3.82IKK8 pKa = 10.52QPLTATSRR16 pKa = 11.84YY17 pKa = 8.73GSNFFASTLFSRR29 pKa = 11.84PEE31 pKa = 3.52RR32 pKa = 11.84SEE34 pKa = 4.03RR35 pKa = 11.84CVAKK39 pKa = 10.56NFYY42 pKa = 10.84LSGRR46 pKa = 11.84KK47 pKa = 8.35NFRR50 pKa = 11.84LDD52 pKa = 2.96RR53 pKa = 11.84HH54 pKa = 6.0SRR56 pKa = 11.84LAAEE60 pKa = 4.43RR61 pKa = 3.88

Molecular weight: 7.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2756

0

2756

933078

30

2771

338.6

38.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.328 ± 0.048

0.891 ± 0.019

4.948 ± 0.032

6.02 ± 0.062

5.257 ± 0.039

6.493 ± 0.047

1.936 ± 0.029

6.98 ± 0.04

6.572 ± 0.075

9.464 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.24 ± 0.028

5.581 ± 0.055

3.926 ± 0.041

4.167 ± 0.033

3.83 ± 0.036

5.92 ± 0.038

6.595 ± 0.101

6.67 ± 0.04

1.156 ± 0.019

4.024 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski