Amino acid dipepetide frequency for Nocardioides sp. PD653

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.92AlaAla: 18.92 ± 0.193
0.986AlaCys: 0.986 ± 0.026
8.631AlaAsp: 8.631 ± 0.077
7.695AlaGlu: 7.695 ± 0.079
3.426AlaPhe: 3.426 ± 0.05
12.235AlaGly: 12.235 ± 0.103
2.616AlaHis: 2.616 ± 0.042
4.663AlaIle: 4.663 ± 0.06
2.577AlaLys: 2.577 ± 0.051
12.996AlaLeu: 12.996 ± 0.119
2.8AlaMet: 2.8 ± 0.04
2.069AlaAsn: 2.069 ± 0.037
6.046AlaPro: 6.046 ± 0.08
3.543AlaGln: 3.543 ± 0.052
9.05AlaArg: 9.05 ± 0.09
6.366AlaSer: 6.366 ± 0.064
7.707AlaThr: 7.707 ± 0.09
11.416AlaVal: 11.416 ± 0.103
1.964AlaTrp: 1.964 ± 0.043
2.472AlaTyr: 2.472 ± 0.042
0.0AlaXaa: 0.0 ± 0.0
Cys
0.896CysAla: 0.896 ± 0.026
0.089CysCys: 0.089 ± 0.007
0.526CysAsp: 0.526 ± 0.021
0.37CysGlu: 0.37 ± 0.015
0.224CysPhe: 0.224 ± 0.012
0.859CysGly: 0.859 ± 0.023
0.188CysHis: 0.188 ± 0.011
0.218CysIle: 0.218 ± 0.013
0.109CysLys: 0.109 ± 0.008
0.693CysLeu: 0.693 ± 0.022
0.114CysMet: 0.114 ± 0.008
0.156CysAsn: 0.156 ± 0.01
0.456CysPro: 0.456 ± 0.018
0.189CysGln: 0.189 ± 0.01
0.552CysArg: 0.552 ± 0.018
0.472CysSer: 0.472 ± 0.018
0.505CysThr: 0.505 ± 0.019
0.631CysVal: 0.631 ± 0.022
0.123CysTrp: 0.123 ± 0.008
0.169CysTyr: 0.169 ± 0.01
0.0CysXaa: 0.0 ± 0.0
Asp
8.046AspAla: 8.046 ± 0.076
0.381AspCys: 0.381 ± 0.017
4.88AspAsp: 4.88 ± 0.08
4.523AspGlu: 4.523 ± 0.062
1.774AspPhe: 1.774 ± 0.038
6.478AspGly: 6.478 ± 0.076
1.658AspHis: 1.658 ± 0.038
2.126AspIle: 2.126 ± 0.038
1.167AspLys: 1.167 ± 0.033
7.511AspLeu: 7.511 ± 0.073
0.893AspMet: 0.893 ± 0.025
1.078AspAsn: 1.078 ± 0.026
4.803AspPro: 4.803 ± 0.058
2.022AspGln: 2.022 ± 0.037
5.016AspArg: 5.016 ± 0.063
2.69AspSer: 2.69 ± 0.05
3.142AspThr: 3.142 ± 0.045
6.174AspVal: 6.174 ± 0.063
1.02AspTrp: 1.02 ± 0.025
1.259AspTyr: 1.259 ± 0.024
0.0AspXaa: 0.0 ± 0.0
Glu
6.839GluAla: 6.839 ± 0.082
0.333GluCys: 0.333 ± 0.016
3.046GluAsp: 3.046 ± 0.052
3.145GluGlu: 3.145 ± 0.055
1.507GluPhe: 1.507 ± 0.033
4.07GluGly: 4.07 ± 0.063
1.654GluHis: 1.654 ± 0.033
2.599GluIle: 2.599 ± 0.036
1.387GluLys: 1.387 ± 0.038
6.47GluLeu: 6.47 ± 0.079
1.087GluMet: 1.087 ± 0.028
0.976GluAsn: 0.976 ± 0.027
3.162GluPro: 3.162 ± 0.058
2.281GluGln: 2.281 ± 0.043
4.884GluArg: 4.884 ± 0.065
2.812GluSer: 2.812 ± 0.041
3.111GluThr: 3.111 ± 0.046
5.172GluVal: 5.172 ± 0.069
0.79GluTrp: 0.79 ± 0.024
1.02GluTyr: 1.02 ± 0.029
0.0GluXaa: 0.0 ± 0.0
Phe
3.557PheAla: 3.557 ± 0.051
0.277PheCys: 0.277 ± 0.013
2.128PheAsp: 2.128 ± 0.04
1.635PheGlu: 1.635 ± 0.031
0.908PhePhe: 0.908 ± 0.028
2.994PheGly: 2.994 ± 0.049
0.624PheHis: 0.624 ± 0.021
0.839PheIle: 0.839 ± 0.023
0.505PheLys: 0.505 ± 0.019
2.572PheLeu: 2.572 ± 0.047
0.432PheMet: 0.432 ± 0.016
0.576PheAsn: 0.576 ± 0.023
1.306PhePro: 1.306 ± 0.032
0.664PheGln: 0.664 ± 0.021
1.729PheArg: 1.729 ± 0.035
1.469PheSer: 1.469 ± 0.034
1.935PheThr: 1.935 ± 0.038
2.704PheVal: 2.704 ± 0.048
0.441PheTrp: 0.441 ± 0.016
0.671PheTyr: 0.671 ± 0.021
0.0PheXaa: 0.0 ± 0.0
Gly
10.134GlyAla: 10.134 ± 0.104
0.845GlyCys: 0.845 ± 0.022
5.74GlyAsp: 5.74 ± 0.077
4.862GlyGlu: 4.862 ± 0.063
2.919GlyPhe: 2.919 ± 0.047
8.082GlyGly: 8.082 ± 0.097
2.175GlyHis: 2.175 ± 0.042
3.792GlyIle: 3.792 ± 0.058
2.141GlyLys: 2.141 ± 0.042
9.366GlyLeu: 9.366 ± 0.074
1.957GlyMet: 1.957 ± 0.037
1.75GlyAsn: 1.75 ± 0.039
4.637GlyPro: 4.637 ± 0.052
2.543GlyGln: 2.543 ± 0.042
7.122GlyArg: 7.122 ± 0.076
5.545GlySer: 5.545 ± 0.064
5.779GlyThr: 5.779 ± 0.074
7.92GlyVal: 7.92 ± 0.08
1.659GlyTrp: 1.659 ± 0.034
2.233GlyTyr: 2.233 ± 0.042
0.0GlyXaa: 0.0 ± 0.0
His
2.621HisAla: 2.621 ± 0.043
0.192HisCys: 0.192 ± 0.012
1.649HisAsp: 1.649 ± 0.032
1.334HisGlu: 1.334 ± 0.028
0.581HisPhe: 0.581 ± 0.022
2.308HisGly: 2.308 ± 0.036
0.763HisHis: 0.763 ± 0.026
0.591HisIle: 0.591 ± 0.022
0.332HisLys: 0.332 ± 0.015
2.435HisLeu: 2.435 ± 0.041
0.33HisMet: 0.33 ± 0.015
0.349HisAsn: 0.349 ± 0.014
1.742HisPro: 1.742 ± 0.04
0.797HisGln: 0.797 ± 0.022
1.993HisArg: 1.993 ± 0.037
0.942HisSer: 0.942 ± 0.025
1.168HisThr: 1.168 ± 0.028
1.954HisVal: 1.954 ± 0.036
0.344HisTrp: 0.344 ± 0.014
0.462HisTyr: 0.462 ± 0.018
0.0HisXaa: 0.0 ± 0.0
Ile
5.246IleAla: 5.246 ± 0.072
0.313IleCys: 0.313 ± 0.014
2.797IleAsp: 2.797 ± 0.042
2.412IleGlu: 2.412 ± 0.039
0.901IlePhe: 0.901 ± 0.028
3.944IleGly: 3.944 ± 0.051
0.7IleHis: 0.7 ± 0.022
1.126IleIle: 1.126 ± 0.031
0.797IleLys: 0.797 ± 0.025
2.761IleLeu: 2.761 ± 0.046
0.471IleMet: 0.471 ± 0.018
0.76IleAsn: 0.76 ± 0.022
1.92IlePro: 1.92 ± 0.034
0.871IleGln: 0.871 ± 0.023
2.403IleArg: 2.403 ± 0.047
2.017IleSer: 2.017 ± 0.037
2.354IleThr: 2.354 ± 0.043
3.407IleVal: 3.407 ± 0.055
0.445IleTrp: 0.445 ± 0.018
0.624IleTyr: 0.624 ± 0.022
0.0IleXaa: 0.0 ± 0.0
Lys
2.658LysAla: 2.658 ± 0.051
0.103LysCys: 0.103 ± 0.009
1.179LysAsp: 1.179 ± 0.026
0.936LysGlu: 0.936 ± 0.025
0.496LysPhe: 0.496 ± 0.022
1.58LysGly: 1.58 ± 0.038
0.491LysHis: 0.491 ± 0.018
0.859LysIle: 0.859 ± 0.027
0.823LysLys: 0.823 ± 0.034
1.81LysLeu: 1.81 ± 0.037
0.393LysMet: 0.393 ± 0.016
0.482LysAsn: 0.482 ± 0.019
1.269LysPro: 1.269 ± 0.032
0.703LysGln: 0.703 ± 0.023
1.383LysArg: 1.383 ± 0.035
1.128LysSer: 1.128 ± 0.028
1.288LysThr: 1.288 ± 0.036
1.953LysVal: 1.953 ± 0.04
0.242LysTrp: 0.242 ± 0.011
0.416LysTyr: 0.416 ± 0.016
0.0LysXaa: 0.0 ± 0.0
Leu
14.529LeuAla: 14.529 ± 0.109
0.749LeuCys: 0.749 ± 0.023
7.205LeuAsp: 7.205 ± 0.082
5.473LeuGlu: 5.473 ± 0.062
2.502LeuPhe: 2.502 ± 0.047
9.37LeuGly: 9.37 ± 0.087
2.103LeuHis: 2.103 ± 0.043
3.107LeuIle: 3.107 ± 0.042
1.674LeuLys: 1.674 ± 0.036
10.386LeuLeu: 10.386 ± 0.107
1.712LeuMet: 1.712 ± 0.037
1.633LeuAsn: 1.633 ± 0.042
5.701LeuPro: 5.701 ± 0.067
2.355LeuGln: 2.355 ± 0.044
7.632LeuArg: 7.632 ± 0.076
5.292LeuSer: 5.292 ± 0.065
6.535LeuThr: 6.535 ± 0.073
10.297LeuVal: 10.297 ± 0.09
1.202LeuTrp: 1.202 ± 0.027
1.534LeuTyr: 1.534 ± 0.031
0.0LeuXaa: 0.0 ± 0.0
Met
2.345MetAla: 2.345 ± 0.04
0.13MetCys: 0.13 ± 0.01
0.903MetAsp: 0.903 ± 0.026
0.767MetGlu: 0.767 ± 0.023
0.502MetPhe: 0.502 ± 0.018
1.389MetGly: 1.389 ± 0.03
0.405MetHis: 0.405 ± 0.017
0.725MetIle: 0.725 ± 0.023
0.496MetLys: 0.496 ± 0.019
1.875MetLeu: 1.875 ± 0.035
0.368MetMet: 0.368 ± 0.016
0.427MetAsn: 0.427 ± 0.016
1.146MetPro: 1.146 ± 0.03
0.486MetGln: 0.486 ± 0.018
1.456MetArg: 1.456 ± 0.033
1.608MetSer: 1.608 ± 0.036
1.844MetThr: 1.844 ± 0.036
1.589MetVal: 1.589 ± 0.031
0.224MetTrp: 0.224 ± 0.011
0.287MetTyr: 0.287 ± 0.014
0.0MetXaa: 0.0 ± 0.0
Asn
2.215AsnAla: 2.215 ± 0.038
0.146AsnCys: 0.146 ± 0.008
1.135AsnAsp: 1.135 ± 0.025
0.859AsnGlu: 0.859 ± 0.023
0.533AsnPhe: 0.533 ± 0.022
1.77AsnGly: 1.77 ± 0.033
0.431AsnHis: 0.431 ± 0.018
0.687AsnIle: 0.687 ± 0.022
0.397AsnLys: 0.397 ± 0.02
1.85AsnLeu: 1.85 ± 0.041
0.294AsnMet: 0.294 ± 0.012
0.423AsnAsn: 0.423 ± 0.019
1.459AsnPro: 1.459 ± 0.029
0.607AsnGln: 0.607 ± 0.021
1.268AsnArg: 1.268 ± 0.027
0.821AsnSer: 0.821 ± 0.024
1.043AsnThr: 1.043 ± 0.028
1.524AsnVal: 1.524 ± 0.036
0.273AsnTrp: 0.273 ± 0.013
0.404AsnTyr: 0.404 ± 0.017
0.0AsnXaa: 0.0 ± 0.0
Pro
7.179ProAla: 7.179 ± 0.09
0.326ProCys: 0.326 ± 0.015
4.671ProAsp: 4.671 ± 0.057
3.841ProGlu: 3.841 ± 0.059
1.533ProPhe: 1.533 ± 0.031
5.805ProGly: 5.805 ± 0.077
1.32ProHis: 1.32 ± 0.029
1.768ProIle: 1.768 ± 0.035
1.074ProLys: 1.074 ± 0.032
4.736ProLeu: 4.736 ± 0.058
1.148ProMet: 1.148 ± 0.029
0.902ProAsn: 0.902 ± 0.026
3.116ProPro: 3.116 ± 0.063
1.497ProGln: 1.497 ± 0.03
3.61ProArg: 3.61 ± 0.07
3.171ProSer: 3.171 ± 0.058
3.717ProThr: 3.717 ± 0.045
5.222ProVal: 5.222 ± 0.064
0.932ProTrp: 0.932 ± 0.024
1.183ProTyr: 1.183 ± 0.033
0.0ProXaa: 0.0 ± 0.0
Gln
3.637GlnAla: 3.637 ± 0.057
0.158GlnCys: 0.158 ± 0.009
1.379GlnAsp: 1.379 ± 0.028
1.445GlnGlu: 1.445 ± 0.032
0.74GlnPhe: 0.74 ± 0.023
2.154GlnGly: 2.154 ± 0.042
0.705GlnHis: 0.705 ± 0.02
1.221GlnIle: 1.221 ± 0.029
0.588GlnLys: 0.588 ± 0.02
3.182GlnLeu: 3.182 ± 0.048
0.581GlnMet: 0.581 ± 0.021
0.484GlnAsn: 0.484 ± 0.02
1.629GlnPro: 1.629 ± 0.036
1.196GlnGln: 1.196 ± 0.03
2.469GlnArg: 2.469 ± 0.044
1.312GlnSer: 1.312 ± 0.033
1.499GlnThr: 1.499 ± 0.028
2.899GlnVal: 2.899 ± 0.037
0.424GlnTrp: 0.424 ± 0.017
0.525GlnTyr: 0.525 ± 0.017
0.0GlnXaa: 0.0 ± 0.0
Arg
8.758ArgAla: 8.758 ± 0.096
0.53ArgCys: 0.53 ± 0.021
4.53ArgAsp: 4.53 ± 0.059
4.273ArgGlu: 4.273 ± 0.063
2.226ArgPhe: 2.226 ± 0.038
5.479ArgGly: 5.479 ± 0.07
1.892ArgHis: 1.892 ± 0.039
3.098ArgIle: 3.098 ± 0.048
1.454ArgLys: 1.454 ± 0.031
7.892ArgLeu: 7.892 ± 0.081
1.755ArgMet: 1.755 ± 0.033
1.325ArgAsn: 1.325 ± 0.029
4.379ArgPro: 4.379 ± 0.069
2.226ArgGln: 2.226 ± 0.044
7.144ArgArg: 7.144 ± 0.099
4.202ArgSer: 4.202 ± 0.06
4.638ArgThr: 4.638 ± 0.058
6.184ArgVal: 6.184 ± 0.064
1.319ArgTrp: 1.319 ± 0.031
1.61ArgTyr: 1.61 ± 0.034
0.0ArgXaa: 0.0 ± 0.0
Ser
6.444SerAla: 6.444 ± 0.073
0.421SerCys: 0.421 ± 0.017
3.228SerAsp: 3.228 ± 0.051
2.577SerGlu: 2.577 ± 0.045
1.72SerPhe: 1.72 ± 0.029
5.74SerGly: 5.74 ± 0.068
1.121SerHis: 1.121 ± 0.028
1.985SerIle: 1.985 ± 0.041
1.045SerLys: 1.045 ± 0.026
5.102SerLeu: 5.102 ± 0.057
1.362SerMet: 1.362 ± 0.028
0.999SerAsn: 0.999 ± 0.025
3.166SerPro: 3.166 ± 0.049
1.441SerGln: 1.441 ± 0.032
3.716SerArg: 3.716 ± 0.048
3.389SerSer: 3.389 ± 0.063
3.537SerThr: 3.537 ± 0.058
4.597SerVal: 4.597 ± 0.052
0.956SerTrp: 0.956 ± 0.025
1.381SerTyr: 1.381 ± 0.032
0.0SerXaa: 0.0 ± 0.0
Thr
7.53ThrAla: 7.53 ± 0.093
0.517ThrCys: 0.517 ± 0.022
3.807ThrAsp: 3.807 ± 0.056
3.06ThrGlu: 3.06 ± 0.049
1.86ThrPhe: 1.86 ± 0.038
6.157ThrGly: 6.157 ± 0.068
1.281ThrHis: 1.281 ± 0.03
2.456ThrIle: 2.456 ± 0.039
1.216ThrLys: 1.216 ± 0.033
5.733ThrLeu: 5.733 ± 0.066
1.175ThrMet: 1.175 ± 0.029
1.231ThrAsn: 1.231 ± 0.031
4.022ThrPro: 4.022 ± 0.057
1.529ThrGln: 1.529 ± 0.034
4.004ThrArg: 4.004 ± 0.063
3.768ThrSer: 3.768 ± 0.05
4.34ThrThr: 4.34 ± 0.062
5.914ThrVal: 5.914 ± 0.074
1.092ThrTrp: 1.092 ± 0.025
1.589ThrTyr: 1.589 ± 0.038
0.0ThrXaa: 0.0 ± 0.0
Val
12.406ValAla: 12.406 ± 0.112
0.714ValCys: 0.714 ± 0.022
6.562ValAsp: 6.562 ± 0.07
5.391ValGlu: 5.391 ± 0.077
2.418ValPhe: 2.418 ± 0.041
7.799ValGly: 7.799 ± 0.072
2.0ValHis: 2.0 ± 0.035
3.318ValIle: 3.318 ± 0.046
1.733ValLys: 1.733 ± 0.034
9.68ValLeu: 9.68 ± 0.083
1.574ValMet: 1.574 ± 0.034
1.766ValAsn: 1.766 ± 0.037
4.989ValPro: 4.989 ± 0.056
2.15ValGln: 2.15 ± 0.04
6.723ValArg: 6.723 ± 0.066
4.881ValSer: 4.881 ± 0.057
5.995ValThr: 5.995 ± 0.083
10.3ValVal: 10.3 ± 0.098
1.184ValTrp: 1.184 ± 0.024
1.551ValTyr: 1.551 ± 0.033
0.0ValXaa: 0.0 ± 0.0
Trp
1.604TrpAla: 1.604 ± 0.033
0.162TrpCys: 0.162 ± 0.01
0.892TrpAsp: 0.892 ± 0.023
0.689TrpGlu: 0.689 ± 0.024
0.545TrpPhe: 0.545 ± 0.02
1.034TrpGly: 1.034 ± 0.025
0.388TrpHis: 0.388 ± 0.017
0.601TrpIle: 0.601 ± 0.023
0.342TrpLys: 0.342 ± 0.016
1.798TrpLeu: 1.798 ± 0.031
0.316TrpMet: 0.316 ± 0.015
0.362TrpAsn: 0.362 ± 0.016
0.775TrpPro: 0.775 ± 0.021
0.535TrpGln: 0.535 ± 0.019
1.246TrpArg: 1.246 ± 0.029
1.039TrpSer: 1.039 ± 0.029
1.03TrpThr: 1.03 ± 0.031
1.289TrpVal: 1.289 ± 0.029
0.374TrpTrp: 0.374 ± 0.017
0.289TrpTyr: 0.289 ± 0.013
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.611TyrAla: 2.611 ± 0.04
0.163TyrCys: 0.163 ± 0.011
1.752TyrAsp: 1.752 ± 0.032
1.058TyrGlu: 1.058 ± 0.025
0.651TyrPhe: 0.651 ± 0.021
1.956TyrGly: 1.956 ± 0.036
0.374TyrHis: 0.374 ± 0.016
0.489TyrIle: 0.489 ± 0.019
0.33TyrLys: 0.33 ± 0.014
2.145TyrLeu: 2.145 ± 0.038
0.21TyrMet: 0.21 ± 0.012
0.392TyrAsn: 0.392 ± 0.015
1.03TyrPro: 1.03 ± 0.023
0.594TyrGln: 0.594 ± 0.019
1.516TyrArg: 1.516 ± 0.033
1.001TyrSer: 1.001 ± 0.024
1.115TyrThr: 1.115 ± 0.028
1.989TyrVal: 1.989 ± 0.039
0.302TyrTrp: 0.302 ± 0.016
0.459TyrTyr: 0.459 ± 0.018
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5032 proteins (1565263 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski