Changjiang sobemo-like virus 1

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1L3KED0|A0A1L3KED0_9VIRU Uncharacterized protein OS=Changjiang sobemo-like virus 1 OX=1922800 PE=4 SV=1
MM1 pKa = 7.51IRR3 pKa = 11.84EE4 pKa = 4.56AFAKK8 pKa = 10.01MNGTAVPGTPYY19 pKa = 11.23AFTYY23 pKa = 9.11PRR25 pKa = 11.84KK26 pKa = 9.94SDD28 pKa = 3.57WIEE31 pKa = 3.8KK32 pKa = 10.38DD33 pKa = 3.7LEE35 pKa = 4.38CLVDD39 pKa = 3.61MVLIRR44 pKa = 11.84LCLLEE49 pKa = 4.35YY50 pKa = 10.52SSSSTVEE57 pKa = 3.48ALYY60 pKa = 8.8NTTLIDD66 pKa = 4.91LGYY69 pKa = 9.51QDD71 pKa = 4.35PVSVFVKK78 pKa = 10.54DD79 pKa = 3.83EE80 pKa = 3.74PHH82 pKa = 6.35TIEE85 pKa = 5.14KK86 pKa = 9.88IKK88 pKa = 10.03QGRR91 pKa = 11.84YY92 pKa = 9.33RR93 pKa = 11.84LIANCTLVEE102 pKa = 4.0EE103 pKa = 4.91AIKK106 pKa = 10.63RR107 pKa = 11.84LLFAEE112 pKa = 4.55QNDD115 pKa = 3.71ADD117 pKa = 5.87KK118 pKa = 11.26NDD120 pKa = 4.38CYY122 pKa = 11.23NEE124 pKa = 4.35CGSAGGWDD132 pKa = 3.37WTTDD136 pKa = 3.08EE137 pKa = 4.05AAARR141 pKa = 11.84CFEE144 pKa = 5.21CIRR147 pKa = 11.84PWLPNAATSDD157 pKa = 3.44VSAWDD162 pKa = 3.48WSVKK166 pKa = 7.62EE167 pKa = 4.05WMFMAEE173 pKa = 3.79LEE175 pKa = 4.01IRR177 pKa = 11.84RR178 pKa = 11.84RR179 pKa = 11.84LNGASTKK186 pKa = 10.51SSWYY190 pKa = 8.9KK191 pKa = 10.08ISYY194 pKa = 9.24NVYY197 pKa = 9.86KK198 pKa = 10.26SFCRR202 pKa = 11.84SVYY205 pKa = 10.81VLTDD209 pKa = 3.52GSAYY213 pKa = 8.95TLEE216 pKa = 4.45HH217 pKa = 6.72PGVQKK222 pKa = 10.99SGDD225 pKa = 3.79YY226 pKa = 9.18NTTTTNGRR234 pKa = 11.84IRR236 pKa = 11.84NLIAVLVGSDD246 pKa = 3.26KK247 pKa = 11.32SKK249 pKa = 11.21SAGDD253 pKa = 4.06DD254 pKa = 4.21NISTWVDD261 pKa = 3.29DD262 pKa = 4.92AVQKK266 pKa = 8.56YY267 pKa = 9.19AKK269 pKa = 10.15YY270 pKa = 9.9GIRR273 pKa = 11.84VTDD276 pKa = 3.45YY277 pKa = 11.17EE278 pKa = 4.14RR279 pKa = 11.84VKK281 pKa = 10.79DD282 pKa = 3.99GKK284 pKa = 11.22VKK286 pKa = 10.41FCSATFTEE294 pKa = 5.26SKK296 pKa = 10.41AIPDD300 pKa = 3.58SAMKK304 pKa = 10.29SLYY307 pKa = 10.06KK308 pKa = 10.57LLWSQPSYY316 pKa = 10.89MLLDD320 pKa = 3.6TFRR323 pKa = 11.84NEE325 pKa = 3.6FRR327 pKa = 11.84HH328 pKa = 6.61APDD331 pKa = 3.85LEE333 pKa = 4.13RR334 pKa = 11.84AEE336 pKa = 4.26AAILRR341 pKa = 11.84SGYY344 pKa = 10.49LADD347 pKa = 4.35LAA349 pKa = 4.89

Molecular weight:
39.58 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1L3KED0|A0A1L3KED0_9VIRU Uncharacterized protein OS=Changjiang sobemo-like virus 1 OX=1922800 PE=4 SV=1
MM1 pKa = 7.39RR2 pKa = 11.84QTMVKK7 pKa = 9.89KK8 pKa = 10.75VKK10 pKa = 10.32ANKK13 pKa = 9.65KK14 pKa = 9.05KK15 pKa = 10.3ALAVKK20 pKa = 10.32SSSSKK25 pKa = 9.64RR26 pKa = 11.84KK27 pKa = 7.94NRR29 pKa = 11.84RR30 pKa = 11.84MKK32 pKa = 9.97TKK34 pKa = 8.94MGIMRR39 pKa = 11.84TEE41 pKa = 4.09GKK43 pKa = 10.23LMKK46 pKa = 10.04AAQLIADD53 pKa = 4.68PCNGPLGYY61 pKa = 10.62GVGDD65 pKa = 4.71GYY67 pKa = 11.59NGAVSEE73 pKa = 4.31RR74 pKa = 11.84QRR76 pKa = 11.84TTIVSPSNNASNVSGYY92 pKa = 9.94IVWFPSYY99 pKa = 10.49HH100 pKa = 6.24GATGVGGNANLYY112 pKa = 9.32YY113 pKa = 10.66FEE115 pKa = 4.48TATPAVAPVNTIANPMGTTVNTSGTFLADD144 pKa = 3.39PTSASISAGGAFTRR158 pKa = 11.84AKK160 pKa = 10.12CLSACVQMAHH170 pKa = 7.03IGTLQTTAGQICTVRR185 pKa = 11.84NLSLAAFNTNYY196 pKa = 8.87GTVSPFQPMSVDD208 pKa = 3.27QLFGYY213 pKa = 9.97AHH215 pKa = 6.51EE216 pKa = 4.17RR217 pKa = 11.84QRR219 pKa = 11.84FNIDD223 pKa = 2.34GHH225 pKa = 4.81EE226 pKa = 4.5VVWRR230 pKa = 11.84PADD233 pKa = 3.4NSSVLRR239 pKa = 11.84SNGADD244 pKa = 3.42PAIATYY250 pKa = 10.29EE251 pKa = 4.01PDD253 pKa = 3.31VLFIQGNPTVNTTSSAVMDD272 pKa = 4.8PSSVMGIAIGFRR284 pKa = 11.84GLSPGLSSTMFNFVKK299 pKa = 10.56CSEE302 pKa = 4.63LEE304 pKa = 3.82LAPRR308 pKa = 11.84NNLMEE313 pKa = 4.53EE314 pKa = 4.21MPRR317 pKa = 11.84PVPGPRR323 pKa = 11.84YY324 pKa = 10.08SISQVTDD331 pKa = 3.22YY332 pKa = 11.02LDD334 pKa = 3.86SVDD337 pKa = 5.16PSWQSHH343 pKa = 5.47LMSKK347 pKa = 10.63LGGAATAAVKK357 pKa = 9.37TFAPTIVDD365 pKa = 3.41MFKK368 pKa = 10.06TKK370 pKa = 10.63LYY372 pKa = 10.43IKK374 pKa = 10.47DD375 pKa = 3.38GSLL378 pKa = 3.28

Molecular weight:
40.43 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1449

349

722

483.0

53.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.248 ± 0.169

1.38 ± 0.357

5.176 ± 0.649

6.694 ± 1.394

3.244 ± 0.142

6.28 ± 0.659

1.035 ± 0.086

4.831 ± 0.193

7.039 ± 0.649

8.696 ± 0.92

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.692 ± 0.458

4.555 ± 0.458

4.762 ± 0.494

2.899 ± 0.33

5.176 ± 0.214

8.282 ± 0.414

5.728 ± 0.961

6.832 ± 0.28

1.311 ± 0.427

4.141 ± 0.385

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski