Changjiang sobemo-like virus 1
Average proteome isoelectric point is 6.68
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KED0|A0A1L3KED0_9VIRU Uncharacterized protein OS=Changjiang sobemo-like virus 1 OX=1922800 PE=4 SV=1
MM1 pKa = 7.51 IRR3 pKa = 11.84 EE4 pKa = 4.56 AFAKK8 pKa = 10.01 MNGTAVPGTPYY19 pKa = 11.23 AFTYY23 pKa = 9.11 PRR25 pKa = 11.84 KK26 pKa = 9.94 SDD28 pKa = 3.57 WIEE31 pKa = 3.8 KK32 pKa = 10.38 DD33 pKa = 3.7 LEE35 pKa = 4.38 CLVDD39 pKa = 3.61 MVLIRR44 pKa = 11.84 LCLLEE49 pKa = 4.35 YY50 pKa = 10.52 SSSSTVEE57 pKa = 3.48 ALYY60 pKa = 8.8 NTTLIDD66 pKa = 4.91 LGYY69 pKa = 9.51 QDD71 pKa = 4.35 PVSVFVKK78 pKa = 10.54 DD79 pKa = 3.83 EE80 pKa = 3.74 PHH82 pKa = 6.35 TIEE85 pKa = 5.14 KK86 pKa = 9.88 IKK88 pKa = 10.03 QGRR91 pKa = 11.84 YY92 pKa = 9.33 RR93 pKa = 11.84 LIANCTLVEE102 pKa = 4.0 EE103 pKa = 4.91 AIKK106 pKa = 10.63 RR107 pKa = 11.84 LLFAEE112 pKa = 4.55 QNDD115 pKa = 3.71 ADD117 pKa = 5.87 KK118 pKa = 11.26 NDD120 pKa = 4.38 CYY122 pKa = 11.23 NEE124 pKa = 4.35 CGSAGGWDD132 pKa = 3.37 WTTDD136 pKa = 3.08 EE137 pKa = 4.05 AAARR141 pKa = 11.84 CFEE144 pKa = 5.21 CIRR147 pKa = 11.84 PWLPNAATSDD157 pKa = 3.44 VSAWDD162 pKa = 3.48 WSVKK166 pKa = 7.62 EE167 pKa = 4.05 WMFMAEE173 pKa = 3.79 LEE175 pKa = 4.01 IRR177 pKa = 11.84 RR178 pKa = 11.84 RR179 pKa = 11.84 LNGASTKK186 pKa = 10.51 SSWYY190 pKa = 8.9 KK191 pKa = 10.08 ISYY194 pKa = 9.24 NVYY197 pKa = 9.86 KK198 pKa = 10.26 SFCRR202 pKa = 11.84 SVYY205 pKa = 10.81 VLTDD209 pKa = 3.52 GSAYY213 pKa = 8.95 TLEE216 pKa = 4.45 HH217 pKa = 6.72 PGVQKK222 pKa = 10.99 SGDD225 pKa = 3.79 YY226 pKa = 9.18 NTTTTNGRR234 pKa = 11.84 IRR236 pKa = 11.84 NLIAVLVGSDD246 pKa = 3.26 KK247 pKa = 11.32 SKK249 pKa = 11.21 SAGDD253 pKa = 4.06 DD254 pKa = 4.21 NISTWVDD261 pKa = 3.29 DD262 pKa = 4.92 AVQKK266 pKa = 8.56 YY267 pKa = 9.19 AKK269 pKa = 10.15 YY270 pKa = 9.9 GIRR273 pKa = 11.84 VTDD276 pKa = 3.45 YY277 pKa = 11.17 EE278 pKa = 4.14 RR279 pKa = 11.84 VKK281 pKa = 10.79 DD282 pKa = 3.99 GKK284 pKa = 11.22 VKK286 pKa = 10.41 FCSATFTEE294 pKa = 5.26 SKK296 pKa = 10.41 AIPDD300 pKa = 3.58 SAMKK304 pKa = 10.29 SLYY307 pKa = 10.06 KK308 pKa = 10.57 LLWSQPSYY316 pKa = 10.89 MLLDD320 pKa = 3.6 TFRR323 pKa = 11.84 NEE325 pKa = 3.6 FRR327 pKa = 11.84 HH328 pKa = 6.61 APDD331 pKa = 3.85 LEE333 pKa = 4.13 RR334 pKa = 11.84 AEE336 pKa = 4.26 AAILRR341 pKa = 11.84 SGYY344 pKa = 10.49 LADD347 pKa = 4.35 LAA349 pKa = 4.89
Molecular weight: 39.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.222
IPC2_protein 5.423
IPC_protein 5.359
Toseland 5.296
ProMoST 5.512
Dawson 5.372
Bjellqvist 5.486
Wikipedia 5.258
Rodwell 5.283
Grimsley 5.232
Solomon 5.372
Lehninger 5.334
Nozaki 5.499
DTASelect 5.677
Thurlkill 5.347
EMBOSS 5.321
Sillero 5.575
Patrickios 3.884
IPC_peptide 5.372
IPC2_peptide 5.563
IPC2.peptide.svr19 5.611
Protein with the highest isoelectric point:
>tr|A0A1L3KED0|A0A1L3KED0_9VIRU Uncharacterized protein OS=Changjiang sobemo-like virus 1 OX=1922800 PE=4 SV=1
MM1 pKa = 7.39 RR2 pKa = 11.84 QTMVKK7 pKa = 9.89 KK8 pKa = 10.75 VKK10 pKa = 10.32 ANKK13 pKa = 9.65 KK14 pKa = 9.05 KK15 pKa = 10.3 ALAVKK20 pKa = 10.32 SSSSKK25 pKa = 9.64 RR26 pKa = 11.84 KK27 pKa = 7.94 NRR29 pKa = 11.84 RR30 pKa = 11.84 MKK32 pKa = 9.97 TKK34 pKa = 8.94 MGIMRR39 pKa = 11.84 TEE41 pKa = 4.09 GKK43 pKa = 10.23 LMKK46 pKa = 10.04 AAQLIADD53 pKa = 4.68 PCNGPLGYY61 pKa = 10.62 GVGDD65 pKa = 4.71 GYY67 pKa = 11.59 NGAVSEE73 pKa = 4.31 RR74 pKa = 11.84 QRR76 pKa = 11.84 TTIVSPSNNASNVSGYY92 pKa = 9.94 IVWFPSYY99 pKa = 10.49 HH100 pKa = 6.24 GATGVGGNANLYY112 pKa = 9.32 YY113 pKa = 10.66 FEE115 pKa = 4.48 TATPAVAPVNTIANPMGTTVNTSGTFLADD144 pKa = 3.39 PTSASISAGGAFTRR158 pKa = 11.84 AKK160 pKa = 10.12 CLSACVQMAHH170 pKa = 7.03 IGTLQTTAGQICTVRR185 pKa = 11.84 NLSLAAFNTNYY196 pKa = 8.87 GTVSPFQPMSVDD208 pKa = 3.27 QLFGYY213 pKa = 9.97 AHH215 pKa = 6.51 EE216 pKa = 4.17 RR217 pKa = 11.84 QRR219 pKa = 11.84 FNIDD223 pKa = 2.34 GHH225 pKa = 4.81 EE226 pKa = 4.5 VVWRR230 pKa = 11.84 PADD233 pKa = 3.4 NSSVLRR239 pKa = 11.84 SNGADD244 pKa = 3.42 PAIATYY250 pKa = 10.29 EE251 pKa = 4.01 PDD253 pKa = 3.31 VLFIQGNPTVNTTSSAVMDD272 pKa = 4.8 PSSVMGIAIGFRR284 pKa = 11.84 GLSPGLSSTMFNFVKK299 pKa = 10.56 CSEE302 pKa = 4.63 LEE304 pKa = 3.82 LAPRR308 pKa = 11.84 NNLMEE313 pKa = 4.53 EE314 pKa = 4.21 MPRR317 pKa = 11.84 PVPGPRR323 pKa = 11.84 YY324 pKa = 10.08 SISQVTDD331 pKa = 3.22 YY332 pKa = 11.02 LDD334 pKa = 3.86 SVDD337 pKa = 5.16 PSWQSHH343 pKa = 5.47 LMSKK347 pKa = 10.63 LGGAATAAVKK357 pKa = 9.37 TFAPTIVDD365 pKa = 3.41 MFKK368 pKa = 10.06 TKK370 pKa = 10.63 LYY372 pKa = 10.43 IKK374 pKa = 10.47 DD375 pKa = 3.38 GSLL378 pKa = 3.28
Molecular weight: 40.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.877
IPC2_protein 8.931
IPC_protein 8.858
Toseland 9.487
ProMoST 9.297
Dawson 9.78
Bjellqvist 9.531
Wikipedia 9.955
Rodwell 10.043
Grimsley 9.853
Solomon 9.809
Lehninger 9.765
Nozaki 9.633
DTASelect 9.487
Thurlkill 9.619
EMBOSS 9.926
Sillero 9.736
Patrickios 4.914
IPC_peptide 9.809
IPC2_peptide 8.244
IPC2.peptide.svr19 7.914
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
1449
349
722
483.0
53.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.248 ± 0.169
1.38 ± 0.357
5.176 ± 0.649
6.694 ± 1.394
3.244 ± 0.142
6.28 ± 0.659
1.035 ± 0.086
4.831 ± 0.193
7.039 ± 0.649
8.696 ± 0.92
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.692 ± 0.458
4.555 ± 0.458
4.762 ± 0.494
2.899 ± 0.33
5.176 ± 0.214
8.282 ± 0.414
5.728 ± 0.961
6.832 ± 0.28
1.311 ± 0.427
4.141 ± 0.385
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here