Amino acid dipepetide frequency for Bradyrhizobiaceae bacterium SG-6C

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	16.906AlaAla: 16.906 ± 0.174	1.028AlaCys: 1.028 ± 0.032	6.649AlaAsp: 6.649 ± 0.08	6.458AlaGlu: 6.458 ± 0.09	4.512AlaPhe: 4.512 ± 0.065	9.807AlaGly: 9.807 ± 0.104	2.156AlaHis: 2.156 ± 0.04	6.813AlaIle: 6.813 ± 0.083	5.352AlaLys: 5.352 ± 0.081	12.45AlaLeu: 12.45 ± 0.129	3.635AlaMet: 3.635 ± 0.07	3.263AlaAsn: 3.263 ± 0.059	5.642AlaPro: 5.642 ± 0.079	4.183AlaGln: 4.183 ± 0.068	8.149AlaArg: 8.149 ± 0.099	6.954AlaSer: 6.954 ± 0.077	6.161AlaThr: 6.161 ± 0.08	8.549AlaVal: 8.549 ± 0.094	1.328AlaTrp: 1.328 ± 0.034	2.545AlaTyr: 2.545 ± 0.054	0.0AlaXaa: 0.0 ± 0.0
Cys	0.989CysAla: 0.989 ± 0.027	0.101CysCys: 0.101 ± 0.011	0.486CysAsp: 0.486 ± 0.021	0.419CysGlu: 0.419 ± 0.017	0.335CysPhe: 0.335 ± 0.015	0.876CysGly: 0.876 ± 0.03	0.196CysHis: 0.196 ± 0.012	0.425CysIle: 0.425 ± 0.019	0.256CysLys: 0.256 ± 0.012	0.68CysLeu: 0.68 ± 0.025	0.187CysMet: 0.187 ± 0.015	0.241CysAsn: 0.241 ± 0.014	0.4CysPro: 0.4 ± 0.018	0.23CysGln: 0.23 ± 0.014	0.548CysArg: 0.548 ± 0.025	0.475CysSer: 0.475 ± 0.023	0.397CysThr: 0.397 ± 0.019	0.64CysVal: 0.64 ± 0.023	0.087CysTrp: 0.087 ± 0.009	0.18CysTyr: 0.18 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	6.593AspAla: 6.593 ± 0.09	0.439AspCys: 0.439 ± 0.021	3.121AspAsp: 3.121 ± 0.057	3.276AspGlu: 3.276 ± 0.062	2.117AspPhe: 2.117 ± 0.042	4.776AspGly: 4.776 ± 0.072	1.242AspHis: 1.242 ± 0.036	3.296AspIle: 3.296 ± 0.06	2.233AspLys: 2.233 ± 0.048	5.479AspLeu: 5.479 ± 0.07	1.358AspMet: 1.358 ± 0.033	1.487AspAsn: 1.487 ± 0.04	3.22AspPro: 3.22 ± 0.051	1.739AspGln: 1.739 ± 0.038	3.942AspArg: 3.942 ± 0.061	2.363AspSer: 2.363 ± 0.04	2.661AspThr: 2.661 ± 0.056	4.327AspVal: 4.327 ± 0.066	0.792AspTrp: 0.792 ± 0.026	1.523AspTyr: 1.523 ± 0.036	0.0AspXaa: 0.0 ± 0.0
Glu	6.498GluAla: 6.498 ± 0.093	0.307GluCys: 0.307 ± 0.016	2.43GluAsp: 2.43 ± 0.048	2.519GluGlu: 2.519 ± 0.049	1.846GluPhe: 1.846 ± 0.042	3.774GluGly: 3.774 ± 0.063	1.065GluHis: 1.065 ± 0.028	3.335GluIle: 3.335 ± 0.052	2.626GluLys: 2.626 ± 0.055	4.717GluLeu: 4.717 ± 0.071	1.499GluMet: 1.499 ± 0.031	1.579GluAsn: 1.579 ± 0.038	2.45GluPro: 2.45 ± 0.048	2.027GluGln: 2.027 ± 0.045	4.44GluArg: 4.44 ± 0.07	2.383GluSer: 2.383 ± 0.049	3.252GluThr: 3.252 ± 0.052	3.529GluVal: 3.529 ± 0.053	0.665GluTrp: 0.665 ± 0.023	0.977GluTyr: 0.977 ± 0.028	0.0GluXaa: 0.0 ± 0.0
Phe	4.854PheAla: 4.854 ± 0.074	0.391PheCys: 0.391 ± 0.02	2.563PheAsp: 2.563 ± 0.038	1.992PheGlu: 1.992 ± 0.037	1.527PhePhe: 1.527 ± 0.049	3.89PheGly: 3.89 ± 0.063	0.716PheHis: 0.716 ± 0.023	1.912PheIle: 1.912 ± 0.05	1.311PheLys: 1.311 ± 0.032	3.337PheLeu: 3.337 ± 0.061	0.835PheMet: 0.835 ± 0.027	1.165PheAsn: 1.165 ± 0.032	1.671PhePro: 1.671 ± 0.04	0.989PheGln: 0.989 ± 0.029	2.274PheArg: 2.274 ± 0.045	2.316PheSer: 2.316 ± 0.043	2.169PheThr: 2.169 ± 0.044	3.017PheVal: 3.017 ± 0.06	0.508PheTrp: 0.508 ± 0.021	0.925PheTyr: 0.925 ± 0.027	0.0PheXaa: 0.0 ± 0.0
Gly	9.022GlyAla: 9.022 ± 0.11	0.798GlyCys: 0.798 ± 0.028	4.292GlyAsp: 4.292 ± 0.07	4.331GlyGlu: 4.331 ± 0.061	3.717GlyPhe: 3.717 ± 0.058	7.461GlyGly: 7.461 ± 0.274	1.832GlyHis: 1.832 ± 0.042	4.906GlyIle: 4.906 ± 0.056	3.867GlyLys: 3.867 ± 0.07	8.378GlyLeu: 8.378 ± 0.089	2.231GlyMet: 2.231 ± 0.043	2.399GlyAsn: 2.399 ± 0.061	3.457GlyPro: 3.457 ± 0.066	2.689GlyGln: 2.689 ± 0.051	5.346GlyArg: 5.346 ± 0.076	4.745GlySer: 4.745 ± 0.09	4.641GlyThr: 4.641 ± 0.087	6.224GlyVal: 6.224 ± 0.08	1.285GlyTrp: 1.285 ± 0.034	2.352GlyTyr: 2.352 ± 0.045	0.0GlyXaa: 0.0 ± 0.0
His	2.285HisAla: 2.285 ± 0.049	0.231HisCys: 0.231 ± 0.013	1.215HisAsp: 1.215 ± 0.03	0.919HisGlu: 0.919 ± 0.029	0.801HisPhe: 0.801 ± 0.024	1.884HisGly: 1.884 ± 0.04	0.628HisHis: 0.628 ± 0.031	1.005HisIle: 1.005 ± 0.029	0.538HisLys: 0.538 ± 0.019	1.877HisLeu: 1.877 ± 0.04	0.479HisMet: 0.479 ± 0.021	0.508HisAsn: 0.508 ± 0.019	1.242HisPro: 1.242 ± 0.033	0.576HisGln: 0.576 ± 0.022	1.398HisArg: 1.398 ± 0.036	0.895HisSer: 0.895 ± 0.025	0.84HisThr: 0.84 ± 0.027	1.467HisVal: 1.467 ± 0.033	0.297HisTrp: 0.297 ± 0.017	0.522HisTyr: 0.522 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	7.983IleAla: 7.983 ± 0.104	0.55IleCys: 0.55 ± 0.021	3.574IleAsp: 3.574 ± 0.057	3.582IleGlu: 3.582 ± 0.06	1.803IlePhe: 1.803 ± 0.041	5.415IleGly: 5.415 ± 0.081	0.963IleHis: 0.963 ± 0.028	2.517IleIle: 2.517 ± 0.053	2.004IleLys: 2.004 ± 0.046	4.568IleLeu: 4.568 ± 0.065	1.133IleMet: 1.133 ± 0.031	1.702IleAsn: 1.702 ± 0.041	2.62IlePro: 2.62 ± 0.047	1.366IleGln: 1.366 ± 0.035	3.191IleArg: 3.191 ± 0.052	3.228IleSer: 3.228 ± 0.056	2.889IleThr: 2.889 ± 0.05	4.906IleVal: 4.906 ± 0.064	0.604IleTrp: 0.604 ± 0.022	1.294IleTyr: 1.294 ± 0.032	0.0IleXaa: 0.0 ± 0.0
Lys	5.025LysAla: 5.025 ± 0.073	0.195LysCys: 0.195 ± 0.014	2.275LysAsp: 2.275 ± 0.047	1.892LysGlu: 1.892 ± 0.043	1.306LysPhe: 1.306 ± 0.039	3.014LysGly: 3.014 ± 0.055	0.758LysHis: 0.758 ± 0.025	2.361LysIle: 2.361 ± 0.049	2.019LysLys: 2.019 ± 0.059	4.076LysLeu: 4.076 ± 0.054	1.078LysMet: 1.078 ± 0.028	1.206LysAsn: 1.206 ± 0.03	2.782LysPro: 2.782 ± 0.049	1.337LysGln: 1.337 ± 0.031	2.787LysArg: 2.787 ± 0.054	2.456LysSer: 2.456 ± 0.043	2.523LysThr: 2.523 ± 0.052	2.851LysVal: 2.851 ± 0.055	0.461LysTrp: 0.461 ± 0.018	0.846LysTyr: 0.846 ± 0.03	0.0LysXaa: 0.0 ± 0.0
Leu	12.421LeuAla: 12.421 ± 0.12	0.831LeuCys: 0.831 ± 0.026	5.535LeuAsp: 5.535 ± 0.074	4.568LeuGlu: 4.568 ± 0.058	3.553LeuPhe: 3.553 ± 0.071	7.826LeuGly: 7.826 ± 0.072	1.728LeuHis: 1.728 ± 0.039	5.332LeuIle: 5.332 ± 0.072	4.219LeuLys: 4.219 ± 0.063	8.733LeuLeu: 8.733 ± 0.1	2.433LeuMet: 2.433 ± 0.047	2.851LeuAsn: 2.851 ± 0.051	5.221LeuPro: 5.221 ± 0.075	2.599LeuGln: 2.599 ± 0.047	6.238LeuArg: 6.238 ± 0.078	6.071LeuSer: 6.071 ± 0.076	5.704LeuThr: 5.704 ± 0.074	7.1LeuVal: 7.1 ± 0.075	1.038LeuTrp: 1.038 ± 0.031	1.923LeuTyr: 1.923 ± 0.047	0.0LeuXaa: 0.0 ± 0.0
Met	3.018MetAla: 3.018 ± 0.057	0.171MetCys: 0.171 ± 0.012	1.222MetAsp: 1.222 ± 0.029	1.038MetGlu: 1.038 ± 0.028	0.844MetPhe: 0.844 ± 0.029	1.762MetGly: 1.762 ± 0.039	0.441MetHis: 0.441 ± 0.02	1.585MetIle: 1.585 ± 0.039	1.23MetLys: 1.23 ± 0.032	2.512MetLeu: 2.512 ± 0.05	0.758MetMet: 0.758 ± 0.028	0.833MetAsn: 0.833 ± 0.025	1.591MetPro: 1.591 ± 0.036	0.885MetGln: 0.885 ± 0.027	1.764MetArg: 1.764 ± 0.036	1.823MetSer: 1.823 ± 0.043	2.068MetThr: 2.068 ± 0.038	1.726MetVal: 1.726 ± 0.037	0.251MetTrp: 0.251 ± 0.014	0.386MetTyr: 0.386 ± 0.019	0.0MetXaa: 0.0 ± 0.0
Asn	3.535AsnAla: 3.535 ± 0.049	0.25AsnCys: 0.25 ± 0.012	1.626AsnAsp: 1.626 ± 0.04	1.394AsnGlu: 1.394 ± 0.033	1.121AsnPhe: 1.121 ± 0.03	2.64AsnGly: 2.64 ± 0.064	0.508AsnHis: 0.508 ± 0.021	1.663AsnIle: 1.663 ± 0.04	0.986AsnLys: 0.986 ± 0.028	2.746AsnLeu: 2.746 ± 0.054	0.678AsnMet: 0.678 ± 0.021	0.907AsnAsn: 0.907 ± 0.033	1.947AsnPro: 1.947 ± 0.042	0.743AsnGln: 0.743 ± 0.024	1.815AsnArg: 1.815 ± 0.038	1.481AsnSer: 1.481 ± 0.038	1.459AsnThr: 1.459 ± 0.04	2.338AsnVal: 2.338 ± 0.043	0.448AsnTrp: 0.448 ± 0.022	0.774AsnTyr: 0.774 ± 0.03	0.0AsnXaa: 0.0 ± 0.0
Pro	6.338ProAla: 6.338 ± 0.092	0.311ProCys: 0.311 ± 0.013	3.518ProAsp: 3.518 ± 0.064	3.256ProGlu: 3.256 ± 0.057	2.017ProPhe: 2.017 ± 0.042	4.404ProGly: 4.404 ± 0.066	1.039ProHis: 1.039 ± 0.03	2.317ProIle: 2.317 ± 0.043	2.281ProLys: 2.281 ± 0.047	4.571ProLeu: 4.571 ± 0.063	1.233ProMet: 1.233 ± 0.032	1.575ProAsn: 1.575 ± 0.037	2.765ProPro: 2.765 ± 0.055	1.755ProGln: 1.755 ± 0.044	3.036ProArg: 3.036 ± 0.058	2.974ProSer: 2.974 ± 0.049	2.42ProThr: 2.42 ± 0.045	4.269ProVal: 4.269 ± 0.061	0.68ProTrp: 0.68 ± 0.027	1.175ProTyr: 1.175 ± 0.034	0.0ProXaa: 0.0 ± 0.0
Gln	3.83GlnAla: 3.83 ± 0.062	0.212GlnCys: 0.212 ± 0.013	1.491GlnAsp: 1.491 ± 0.041	1.285GlnGlu: 1.285 ± 0.034	1.122GlnPhe: 1.122 ± 0.027	2.305GlnGly: 2.305 ± 0.048	0.615GlnHis: 0.615 ± 0.021	1.968GlnIle: 1.968 ± 0.043	1.33GlnLys: 1.33 ± 0.032	2.855GlnLeu: 2.855 ± 0.054	0.913GlnMet: 0.913 ± 0.024	0.936GlnAsn: 0.936 ± 0.027	1.797GlnPro: 1.797 ± 0.053	1.298GlnGln: 1.298 ± 0.039	2.401GlnArg: 2.401 ± 0.05	1.872GlnSer: 1.872 ± 0.047	1.871GlnThr: 1.871 ± 0.042	2.13GlnVal: 2.13 ± 0.038	0.411GlnTrp: 0.411 ± 0.018	0.666GlnTyr: 0.666 ± 0.023	0.0GlnXaa: 0.0 ± 0.0
Arg	7.543ArgAla: 7.543 ± 0.091	0.479ArgCys: 0.479 ± 0.02	4.025ArgAsp: 4.025 ± 0.062	3.94ArgGlu: 3.94 ± 0.069	2.688ArgPhe: 2.688 ± 0.052	4.722ArgGly: 4.722 ± 0.067	1.511ArgHis: 1.511 ± 0.037	3.965ArgIle: 3.965 ± 0.061	2.855ArgLys: 2.855 ± 0.048	6.767ArgLeu: 6.767 ± 0.087	1.856ArgMet: 1.856 ± 0.043	2.04ArgAsn: 2.04 ± 0.037	3.25ArgPro: 3.25 ± 0.065	2.233ArgGln: 2.233 ± 0.043	4.824ArgArg: 4.824 ± 0.09	3.538ArgSer: 3.538 ± 0.057	3.291ArgThr: 3.291 ± 0.053	4.584ArgVal: 4.584 ± 0.064	0.889ArgTrp: 0.889 ± 0.029	1.615ArgTyr: 1.615 ± 0.043	0.0ArgXaa: 0.0 ± 0.0
Ser	6.51SerAla: 6.51 ± 0.087	0.415SerCys: 0.415 ± 0.019	3.184SerAsp: 3.184 ± 0.054	2.78SerGlu: 2.78 ± 0.053	2.414SerPhe: 2.414 ± 0.045	5.713SerGly: 5.713 ± 0.082	1.109SerHis: 1.109 ± 0.032	3.188SerIle: 3.188 ± 0.065	2.029SerLys: 2.029 ± 0.043	5.43SerLeu: 5.43 ± 0.073	1.402SerMet: 1.402 ± 0.038	1.613SerAsn: 1.613 ± 0.037	3.048SerPro: 3.048 ± 0.05	1.747SerGln: 1.747 ± 0.039	3.668SerArg: 3.668 ± 0.059	3.417SerSer: 3.417 ± 0.059	2.747SerThr: 2.747 ± 0.05	4.25SerVal: 4.25 ± 0.065	0.704SerTrp: 0.704 ± 0.021	1.389SerTyr: 1.389 ± 0.032	0.0SerXaa: 0.0 ± 0.0
Thr	6.322ThrAla: 6.322 ± 0.076	0.444ThrCys: 0.444 ± 0.019	2.785ThrAsp: 2.785 ± 0.045	2.598ThrGlu: 2.598 ± 0.05	2.291ThrPhe: 2.291 ± 0.044	5.246ThrGly: 5.246 ± 0.075	0.991ThrHis: 0.991 ± 0.025	3.156ThrIle: 3.156 ± 0.056	1.901ThrLys: 1.901 ± 0.042	5.667ThrLeu: 5.667 ± 0.067	1.35ThrMet: 1.35 ± 0.034	1.463ThrAsn: 1.463 ± 0.037	3.316ThrPro: 3.316 ± 0.052	1.553ThrGln: 1.553 ± 0.034	3.298ThrArg: 3.298 ± 0.058	3.194ThrSer: 3.194 ± 0.052	3.076ThrThr: 3.076 ± 0.063	4.283ThrVal: 4.283 ± 0.071	0.644ThrTrp: 0.644 ± 0.024	1.27ThrTyr: 1.27 ± 0.034	0.0ThrXaa: 0.0 ± 0.0
Val	9.265ValAla: 9.265 ± 0.084	0.627ValCys: 0.627 ± 0.021	3.963ValAsp: 3.963 ± 0.065	4.035ValGlu: 4.035 ± 0.069	2.831ValPhe: 2.831 ± 0.056	5.409ValGly: 5.409 ± 0.082	1.368ValHis: 1.368 ± 0.03	4.436ValIle: 4.436 ± 0.071	2.883ValLys: 2.883 ± 0.054	7.296ValLeu: 7.296 ± 0.08	2.033ValMet: 2.033 ± 0.04	2.213ValAsn: 2.213 ± 0.045	3.726ValPro: 3.726 ± 0.054	2.097ValGln: 2.097 ± 0.042	4.743ValArg: 4.743 ± 0.073	4.54ValSer: 4.54 ± 0.068	4.719ValThr: 4.719 ± 0.074	6.056ValVal: 6.056 ± 0.091	0.891ValTrp: 0.891 ± 0.03	1.523ValTyr: 1.523 ± 0.033	0.0ValXaa: 0.0 ± 0.0
Trp	1.078TrpAla: 1.078 ± 0.024	0.14TrpCys: 0.14 ± 0.012	0.574TrpAsp: 0.574 ± 0.024	0.481TrpGlu: 0.481 ± 0.019	0.521TrpPhe: 0.521 ± 0.019	0.845TrpGly: 0.845 ± 0.029	0.303TrpHis: 0.303 ± 0.015	0.744TrpIle: 0.744 ± 0.026	0.512TrpLys: 0.512 ± 0.019	1.518TrpLeu: 1.518 ± 0.04	0.375TrpMet: 0.375 ± 0.019	0.452TrpAsn: 0.452 ± 0.02	0.665TrpPro: 0.665 ± 0.027	0.502TrpGln: 0.502 ± 0.02	1.075TrpArg: 1.075 ± 0.033	0.805TrpSer: 0.805 ± 0.025	0.784TrpThr: 0.784 ± 0.027	0.725TrpVal: 0.725 ± 0.02	0.207TrpTrp: 0.207 ± 0.013	0.247TrpTyr: 0.247 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.525TyrAla: 2.525 ± 0.052	0.23TyrCys: 0.23 ± 0.013	1.452TyrAsp: 1.452 ± 0.036	1.158TyrGlu: 1.158 ± 0.036	0.995TyrPhe: 0.995 ± 0.035	2.205TyrGly: 2.205 ± 0.049	0.44TyrHis: 0.44 ± 0.019	0.968TyrIle: 0.968 ± 0.028	0.815TyrLys: 0.815 ± 0.026	2.267TyrLeu: 2.267 ± 0.044	0.439TyrMet: 0.439 ± 0.019	0.625TyrAsn: 0.625 ± 0.023	1.122TyrPro: 1.122 ± 0.033	0.76TyrGln: 0.76 ± 0.025	1.681TyrArg: 1.681 ± 0.039	1.229TyrSer: 1.229 ± 0.032	1.174TyrThr: 1.174 ± 0.031	1.681TyrVal: 1.681 ± 0.041	0.363TyrTrp: 0.363 ± 0.019	0.649TyrTyr: 0.649 ± 0.029	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4236 proteins (1249219 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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