Chitinivibrio alkaliphilus ACht1
Average proteome isoelectric point is 6.19
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2304 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|U7DDA3|U7DDA3_9BACT Pyruvate:feredoxin oxidoreductase OS=Chitinivibrio alkaliphilus ACht1 OX=1313304 GN=CALK_0639 PE=3 SV=1
MM1 pKa = 7.48 KK2 pKa = 10.41 FLVFCITFFSFVLLAGCARR21 pKa = 11.84 EE22 pKa = 4.02 HH23 pKa = 7.13 DD24 pKa = 4.81 VIGDD28 pKa = 3.44 PHH30 pKa = 7.54 MDD32 pKa = 3.25 EE33 pKa = 4.55 PAATEE38 pKa = 4.87 PYY40 pKa = 10.0 TGDD43 pKa = 3.32 TGEE46 pKa = 5.08 EE47 pKa = 4.35 EE48 pKa = 4.52 PPMDD52 pKa = 5.22 DD53 pKa = 3.29 PHH55 pKa = 7.71 GDD57 pKa = 3.18 PHH59 pKa = 7.4 MDD61 pKa = 4.12 DD62 pKa = 3.69 PHH64 pKa = 7.73 GDD66 pKa = 3.22 PHH68 pKa = 7.4 MDD70 pKa = 4.12 DD71 pKa = 3.69 PHH73 pKa = 7.73 GDD75 pKa = 3.22 PHH77 pKa = 7.4 MDD79 pKa = 4.12 DD80 pKa = 3.69 PHH82 pKa = 7.73 GDD84 pKa = 3.22 PHH86 pKa = 7.4 MDD88 pKa = 4.12 DD89 pKa = 3.69 PHH91 pKa = 7.73 GDD93 pKa = 3.22 PHH95 pKa = 7.4 MDD97 pKa = 4.12 DD98 pKa = 3.69 PHH100 pKa = 7.73 GDD102 pKa = 3.22 PHH104 pKa = 7.4 MDD106 pKa = 4.12 DD107 pKa = 3.69 PHH109 pKa = 7.71 GDD111 pKa = 3.22 PHH113 pKa = 7.43 MDD115 pKa = 3.55 EE116 pKa = 4.46 PHH118 pKa = 7.21 GDD120 pKa = 3.16 PHH122 pKa = 7.75 MDD124 pKa = 4.02 DD125 pKa = 3.39 QHH127 pKa = 6.71 MDD129 pKa = 3.22 APQLDD134 pKa = 4.54 DD135 pKa = 3.38 QHH137 pKa = 7.31 MDD139 pKa = 3.66 DD140 pKa = 4.36 PHH142 pKa = 8.48 DD143 pKa = 4.8 GGDD146 pKa = 3.48 EE147 pKa = 4.05 LQIQQ151 pKa = 3.78
Molecular weight: 16.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.767
IPC2_protein 3.948
IPC_protein 3.986
Toseland 3.757
ProMoST 4.177
Dawson 4.012
Bjellqvist 4.151
Wikipedia 3.973
Rodwell 3.821
Grimsley 3.668
Solomon 3.999
Lehninger 3.961
Nozaki 4.113
DTASelect 4.431
Thurlkill 3.821
EMBOSS 3.986
Sillero 4.126
Patrickios 1.964
IPC_peptide 3.999
IPC2_peptide 4.101
IPC2.peptide.svr19 3.986
Protein with the highest isoelectric point:
>tr|U7D9U2|U7D9U2_9BACT Cell division protein FtsQ OS=Chitinivibrio alkaliphilus ACht1 OX=1313304 GN=CALK_1908 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNRR10 pKa = 11.84 KK11 pKa = 9.29 KK12 pKa = 9.89 INKK15 pKa = 9.12 HH16 pKa = 4.13 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MATKK25 pKa = 10.49 NGRR28 pKa = 11.84 KK29 pKa = 7.92 VLKK32 pKa = 10.05 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.61 GRR39 pKa = 11.84 KK40 pKa = 8.54 RR41 pKa = 11.84 LSVSS45 pKa = 2.92
Molecular weight: 5.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.515
IPC2_protein 11.228
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.749
Grimsley 13.013
Solomon 13.466
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.486
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.134
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2304
0
2304
774486
20
2622
336.1
37.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.479 ± 0.051
1.113 ± 0.021
5.647 ± 0.051
7.259 ± 0.057
4.49 ± 0.037
6.96 ± 0.051
2.616 ± 0.026
6.677 ± 0.047
4.514 ± 0.057
9.614 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.355 ± 0.019
3.424 ± 0.03
4.157 ± 0.028
3.516 ± 0.025
5.978 ± 0.046
6.98 ± 0.043
6.052 ± 0.039
6.543 ± 0.046
1.002 ± 0.02
3.626 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here