Phomopsis vexans RNA virus
Average proteome isoelectric point is 6.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A7RU00|A0A0A7RU00_9VIRU Coat protein OS=Phomopsis vexans RNA virus OX=1580605 GN=CP PE=4 SV=1
MM1 pKa = 7.73 ASTANFQAAVSSMLSGTLAGVPGGLLQQEE30 pKa = 4.14 DD31 pKa = 4.5 RR32 pKa = 11.84 YY33 pKa = 10.63 RR34 pKa = 11.84 RR35 pKa = 11.84 YY36 pKa = 10.25 RR37 pKa = 11.84 SGLSIGVQEE46 pKa = 4.96 HH47 pKa = 6.55 GSLTYY52 pKa = 10.04 SRR54 pKa = 11.84 RR55 pKa = 11.84 SIFYY59 pKa = 8.6 EE60 pKa = 3.74 VGRR63 pKa = 11.84 RR64 pKa = 11.84 TPRR67 pKa = 11.84 LNDD70 pKa = 3.19 ALAFTKK76 pKa = 10.44 NDD78 pKa = 3.42 EE79 pKa = 4.66 EE80 pKa = 4.27 ITMVDD85 pKa = 2.89 ASVPINPAQAANFEE99 pKa = 4.05 GWARR103 pKa = 11.84 KK104 pKa = 9.44 YY105 pKa = 11.34 SNFSPQWEE113 pKa = 4.33 MMDD116 pKa = 3.25 LAGIVEE122 pKa = 4.63 RR123 pKa = 11.84 LAKK126 pKa = 10.16 AVAAQSVYY134 pKa = 11.26 GGVTCANLRR143 pKa = 11.84 AGQPVRR149 pKa = 11.84 VVALGTLDD157 pKa = 3.77 SPQTASVNSVFIPRR171 pKa = 11.84 TVEE174 pKa = 3.8 TTTSDD179 pKa = 3.11 GVFAVLSAAANGEE192 pKa = 4.36 GASVTTDD199 pKa = 3.22 VLRR202 pKa = 11.84 LDD204 pKa = 4.51 AGTNQPIVPTVDD216 pKa = 2.95 NAVLATSCVEE226 pKa = 3.78 ALRR229 pKa = 11.84 ILGANLEE236 pKa = 4.19 EE237 pKa = 4.99 SGAGDD242 pKa = 3.6 VFALAVTRR250 pKa = 11.84 GVHH253 pKa = 5.6 SVLSVVGHH261 pKa = 5.49 TDD263 pKa = 2.69 EE264 pKa = 5.28 GGWIRR269 pKa = 11.84 GVFRR273 pKa = 11.84 HH274 pKa = 5.41 CTFRR278 pKa = 11.84 VPYY281 pKa = 10.51 GGINPSLRR289 pKa = 11.84 EE290 pKa = 4.08 YY291 pKa = 10.56 PGLPPLASTLTGHH304 pKa = 6.94 ISAWCDD310 pKa = 3.48 AIALKK315 pKa = 10.6 SAAAVAHH322 pKa = 6.82 CDD324 pKa = 3.38 PCVTASGGTYY334 pKa = 7.97 PTVFTSNRR342 pKa = 11.84 GLVSPPGTDD351 pKa = 3.18 EE352 pKa = 5.66 ADD354 pKa = 4.17 LGDD357 pKa = 4.47 ADD359 pKa = 4.1 ADD361 pKa = 3.71 AVGRR365 pKa = 11.84 QVGADD370 pKa = 3.71 LGRR373 pKa = 11.84 FSPVYY378 pKa = 9.63 MDD380 pKa = 4.42 ALTTIFGLRR389 pKa = 11.84 AVSGVADD396 pKa = 3.5 AVFRR400 pKa = 11.84 TVGRR404 pKa = 11.84 DD405 pKa = 3.73 YY406 pKa = 11.7 LNGAHH411 pKa = 7.13 DD412 pKa = 3.22 RR413 pKa = 11.84 HH414 pKa = 6.18 LRR416 pKa = 11.84 HH417 pKa = 5.88 KK418 pKa = 9.26 TVAPYY423 pKa = 9.8 FWIEE427 pKa = 3.9 PTSLIPAGAFGTVAEE442 pKa = 4.28 QAGFGALTTPGVEE455 pKa = 3.99 RR456 pKa = 11.84 EE457 pKa = 3.91 QPAFEE462 pKa = 4.32 KK463 pKa = 10.83 VRR465 pKa = 11.84 EE466 pKa = 4.05 LDD468 pKa = 2.87 HH469 pKa = 6.92 GRR471 pKa = 11.84 NANYY475 pKa = 8.85 ATIAFKK481 pKa = 10.22 MRR483 pKa = 11.84 SARR486 pKa = 11.84 TSGLICAYY494 pKa = 10.1 AGTPASLTGLRR505 pKa = 11.84 LYY507 pKa = 10.74 QFDD510 pKa = 4.06 EE511 pKa = 4.6 EE512 pKa = 5.04 SVVLAGDD519 pKa = 3.35 QGPTGGRR526 pKa = 11.84 VAQKK530 pKa = 10.12 HH531 pKa = 5.12 AAADD535 pKa = 4.59 PISSYY540 pKa = 9.56 LWRR543 pKa = 11.84 RR544 pKa = 11.84 GQSPIPAPAEE554 pKa = 3.84 FVNTQGSYY562 pKa = 8.35 AAKK565 pKa = 9.93 YY566 pKa = 10.21 KK567 pKa = 10.16 IVEE570 pKa = 3.9 WDD572 pKa = 3.31 EE573 pKa = 5.79 DD574 pKa = 3.81 FDD576 pKa = 4.1 ATLGDD581 pKa = 3.66 LPEE584 pKa = 4.77 AFEE587 pKa = 4.52 LEE589 pKa = 4.48 QYY591 pKa = 8.96 SVRR594 pKa = 11.84 WRR596 pKa = 11.84 VTVPTGLPNGPSNAGDD612 pKa = 3.52 SGAKK616 pKa = 9.33 RR617 pKa = 11.84 ARR619 pKa = 11.84 SRR621 pKa = 11.84 AAISLAQATIRR632 pKa = 11.84 NRR634 pKa = 11.84 GLGDD638 pKa = 3.55 ANSPVISVSNVPPSWEE654 pKa = 4.3 DD655 pKa = 3.41 EE656 pKa = 4.35 PGRR659 pKa = 11.84 PMAADD664 pKa = 3.19 IGDD667 pKa = 3.88 HH668 pKa = 5.9 RR669 pKa = 11.84 VGVADD674 pKa = 3.93 GQVYY678 pKa = 10.51 ASGADD683 pKa = 3.13 RR684 pKa = 11.84 HH685 pKa = 5.95 VRR687 pKa = 11.84 GDD689 pKa = 3.6 DD690 pKa = 3.1 RR691 pKa = 11.84 GAALNPVAHH700 pKa = 6.23 HH701 pKa = 5.73 QPLRR705 pKa = 11.84 GAPYY709 pKa = 8.44 PPRR712 pKa = 11.84 GGGVLPGGGGPALPPQPPAGPPPAAPPSGGDD743 pKa = 3.09 NGSDD747 pKa = 3.23 VSGPANEE754 pKa = 4.36 PDD756 pKa = 3.81 AGNAAPAPPLL766 pKa = 3.63
Molecular weight: 80.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.416
IPC2_protein 5.372
IPC_protein 5.385
Toseland 5.626
ProMoST 5.652
Dawson 5.537
Bjellqvist 5.601
Wikipedia 5.486
Rodwell 5.486
Grimsley 5.715
Solomon 5.537
Lehninger 5.512
Nozaki 5.741
DTASelect 5.931
Thurlkill 5.792
EMBOSS 5.753
Sillero 5.817
Patrickios 4.139
IPC_peptide 5.55
IPC2_peptide 5.83
IPC2.peptide.svr19 5.788
Protein with the highest isoelectric point:
>tr|A0A0A7RU00|A0A0A7RU00_9VIRU Coat protein OS=Phomopsis vexans RNA virus OX=1580605 GN=CP PE=4 SV=1
MM1 pKa = 7.9 PFAQQISFVYY11 pKa = 9.89 RR12 pKa = 11.84 PRR14 pKa = 11.84 WNNTVPSRR22 pKa = 11.84 LARR25 pKa = 11.84 SAASFLHH32 pKa = 6.48 CFVPVQVPLEE42 pKa = 4.04 QGDD45 pKa = 4.51 LDD47 pKa = 4.75 LLTGQLCGHH56 pKa = 7.15 LTMPPDD62 pKa = 3.81 AGFSSMVVDD71 pKa = 4.43 RR72 pKa = 11.84 DD73 pKa = 3.05 EE74 pKa = 5.7 AMAAFPPKK82 pKa = 9.65 NYY84 pKa = 10.81 AMAIEE89 pKa = 4.28 KK90 pKa = 10.84 ANIYY94 pKa = 9.83 VDD96 pKa = 4.42 EE97 pKa = 4.88 IARR100 pKa = 11.84 DD101 pKa = 4.03 MYY103 pKa = 11.27 KK104 pKa = 8.68 HH105 pKa = 6.08 TPTEE109 pKa = 3.92 LLRR112 pKa = 11.84 AGPYY116 pKa = 9.96 LRR118 pKa = 11.84 RR119 pKa = 11.84 LRR121 pKa = 11.84 DD122 pKa = 3.09 KK123 pKa = 11.27 GVTHH127 pKa = 6.97 DD128 pKa = 3.58 QAAGFLLYY136 pKa = 10.61 AATLSRR142 pKa = 11.84 WCPHH146 pKa = 5.83 AYY148 pKa = 9.16 EE149 pKa = 3.82 WAYY152 pKa = 10.93 YY153 pKa = 10.64 AITDD157 pKa = 3.83 PAAAKK162 pKa = 9.8 EE163 pKa = 4.07 VSDD166 pKa = 3.73 YY167 pKa = 11.48 LKK169 pKa = 11.0 AVGANSSSYY178 pKa = 10.89 GALLVEE184 pKa = 4.5 TDD186 pKa = 3.46 TLQGRR191 pKa = 11.84 GVPGTDD197 pKa = 4.26 LPSDD201 pKa = 3.53 SAKK204 pKa = 10.36 RR205 pKa = 11.84 CNLAALKK212 pKa = 10.52 EE213 pKa = 4.14 DD214 pKa = 3.96 MLAEE218 pKa = 4.77 LDD220 pKa = 4.01 PVALRR225 pKa = 11.84 SAVRR229 pKa = 11.84 RR230 pKa = 11.84 VLRR233 pKa = 11.84 HH234 pKa = 5.34 EE235 pKa = 3.98 LRR237 pKa = 11.84 HH238 pKa = 6.29 RR239 pKa = 11.84 DD240 pKa = 3.13 GNYY243 pKa = 10.82 ALDD246 pKa = 4.09 FPTLEE251 pKa = 4.42 EE252 pKa = 4.17 HH253 pKa = 6.85 WSSRR257 pKa = 11.84 WSWAVNGSHH266 pKa = 6.71 SSMVSKK272 pKa = 8.94 TVPMVGVRR280 pKa = 11.84 GKK282 pKa = 9.75 HH283 pKa = 3.3 VHH285 pKa = 6.09 KK286 pKa = 9.74 YY287 pKa = 9.53 HH288 pKa = 6.37 RR289 pKa = 11.84 RR290 pKa = 11.84 AWLEE294 pKa = 3.78 SVRR297 pKa = 11.84 DD298 pKa = 3.79 DD299 pKa = 5.13 PRR301 pKa = 11.84 PNWEE305 pKa = 3.5 GKK307 pKa = 8.14 TFVSCNPKK315 pKa = 10.27 LEE317 pKa = 4.43 CGKK320 pKa = 8.26 TRR322 pKa = 11.84 AIYY325 pKa = 10.51 ACDD328 pKa = 3.34 TVSYY332 pKa = 10.56 LAFEE336 pKa = 4.62 HH337 pKa = 6.75 LMAPVEE343 pKa = 4.23 RR344 pKa = 11.84 AWQGRR349 pKa = 11.84 RR350 pKa = 11.84 ILLNPGKK357 pKa = 10.35 GGSIGLAEE365 pKa = 4.0 RR366 pKa = 11.84 VKK368 pKa = 10.44 RR369 pKa = 11.84 CRR371 pKa = 11.84 DD372 pKa = 3.22 RR373 pKa = 11.84 SGVSMMLDD381 pKa = 3.24 YY382 pKa = 11.46 TDD384 pKa = 5.3 FNSHH388 pKa = 5.96 HH389 pKa = 5.94 TTSAQQILIEE399 pKa = 4.33 EE400 pKa = 4.54 TCALTGYY407 pKa = 9.96 PPDD410 pKa = 3.59 LTAKK414 pKa = 10.23 LVRR417 pKa = 11.84 SFEE420 pKa = 4.07 LQDD423 pKa = 3.24 IYY425 pKa = 11.56 LSGKK429 pKa = 9.8 RR430 pKa = 11.84 VGRR433 pKa = 11.84 SAGTLMSGHH442 pKa = 7.01 RR443 pKa = 11.84 CTTYY447 pKa = 10.57 INSVLNMAYY456 pKa = 10.81 LMVVLGEE463 pKa = 4.27 DD464 pKa = 3.87 FVLEE468 pKa = 4.13 RR469 pKa = 11.84 QSLHH473 pKa = 7.28 VGDD476 pKa = 5.16 DD477 pKa = 3.73 VYY479 pKa = 11.44 LGARR483 pKa = 11.84 DD484 pKa = 3.83 YY485 pKa = 11.23 PDD487 pKa = 4.04 AGHH490 pKa = 7.54 IITEE494 pKa = 4.28 VMRR497 pKa = 11.84 SPLRR501 pKa = 11.84 MNKK504 pKa = 9.08 SKK506 pKa = 10.94 QSVGHH511 pKa = 6.22 VGTEE515 pKa = 3.9 LTRR518 pKa = 11.84 VASNKK523 pKa = 9.33 RR524 pKa = 11.84 DD525 pKa = 3.47 SFGYY529 pKa = 9.96 LARR532 pKa = 11.84 ATANLIAGNWYY543 pKa = 9.62 SDD545 pKa = 3.35 SVMDD549 pKa = 4.5 PLEE552 pKa = 4.47 GFTTMLAGCRR562 pKa = 11.84 TLANRR567 pKa = 11.84 GHH569 pKa = 6.15 GMKK572 pKa = 10.49 APLLLEE578 pKa = 4.26 SAVKK582 pKa = 10.47 RR583 pKa = 11.84 ILGDD587 pKa = 3.7 DD588 pKa = 4.22 CPDD591 pKa = 3.7 DD592 pKa = 4.15 ALLRR596 pKa = 11.84 RR597 pKa = 11.84 ILLGDD602 pKa = 3.31 IAVNSGPQYY611 pKa = 9.97 SSSGRR616 pKa = 11.84 HH617 pKa = 5.01 VSIQVKK623 pKa = 10.77 AEE625 pKa = 3.68 FTTADD630 pKa = 3.47 DD631 pKa = 3.98 FGYY634 pKa = 11.06 SLVPKK639 pKa = 10.27 LATTTYY645 pKa = 9.63 LSSHH649 pKa = 6.99 ASPLEE654 pKa = 3.49 IRR656 pKa = 11.84 TLTEE660 pKa = 3.82 CGIDD664 pKa = 3.54 PSPSMVRR671 pKa = 11.84 SSWKK675 pKa = 9.28 KK676 pKa = 8.75 SLRR679 pKa = 11.84 IKK681 pKa = 10.32 DD682 pKa = 3.41 RR683 pKa = 11.84 CLEE686 pKa = 3.98 RR687 pKa = 11.84 LVFGAVTTRR696 pKa = 11.84 PARR699 pKa = 11.84 GSIRR703 pKa = 11.84 AEE705 pKa = 4.2 DD706 pKa = 4.29 LLKK709 pKa = 10.45 QPKK712 pKa = 9.09 PHH714 pKa = 6.55 GCLLQFPLLVLAKK727 pKa = 10.51 DD728 pKa = 4.66 RR729 pKa = 11.84 IPDD732 pKa = 3.58 SEE734 pKa = 4.26 LRR736 pKa = 11.84 RR737 pKa = 11.84 AVSDD741 pKa = 3.13 SGGRR745 pKa = 11.84 GWVEE749 pKa = 4.46 DD750 pKa = 3.86 IKK752 pKa = 11.45 LEE754 pKa = 4.04 AWGEE758 pKa = 3.99 YY759 pKa = 8.43 HH760 pKa = 7.53 PSMIVNTGMSFSDD773 pKa = 3.33 AAALSKK779 pKa = 10.43 RR780 pKa = 11.84 TDD782 pKa = 3.1 ISVMTSTRR790 pKa = 11.84 RR791 pKa = 11.84 CYY793 pKa = 10.31 VV794 pKa = 2.8
Molecular weight: 88.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.635
IPC2_protein 7.922
IPC_protein 7.732
Toseland 7.424
ProMoST 8.317
Dawson 8.463
Bjellqvist 8.814
Wikipedia 8.404
Rodwell 8.492
Grimsley 7.497
Solomon 8.507
Lehninger 8.521
Nozaki 8.96
DTASelect 8.536
Thurlkill 8.595
EMBOSS 8.653
Sillero 8.902
Patrickios 4.406
IPC_peptide 8.507
IPC2_peptide 7.805
IPC2.peptide.svr19 7.918
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1560
766
794
780.0
84.11
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.282 ± 1.53
1.474 ± 0.374
6.026 ± 0.188
4.487 ± 0.119
2.756 ± 0.164
8.974 ± 1.414
2.5 ± 0.361
3.269 ± 0.091
2.821 ± 0.923
8.782 ± 1.155
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.051 ± 0.671
3.077 ± 0.298
6.538 ± 0.95
2.564 ± 0.292
7.5 ± 0.474
7.756 ± 0.471
5.833 ± 0.115
7.692 ± 0.529
1.41 ± 0.157
3.205 ± 0.309
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here