Chitinophaga sp. MD30

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Chitinophagia; Chitinophagales; Chitinophagaceae; Chitinophaga; unclassified Chitinophaga

Average proteome isoelectric point is 6.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5689 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A249T0A3|A0A249T0A3_9BACT NigD_N domain-containing protein OS=Chitinophaga sp. MD30 OX=2033437 GN=CK934_21350 PE=4 SV=1
MM1 pKa = 6.89VASLVTAPVNGTVVLNANGSFTYY24 pKa = 10.1TPNEE28 pKa = 4.05NFNGTDD34 pKa = 2.94SLTYY38 pKa = 8.76QVCDD42 pKa = 2.98NGTPSLCDD50 pKa = 2.92TATVVFNVAAINDD63 pKa = 3.78APVAVRR69 pKa = 11.84DD70 pKa = 4.21TVAVTEE76 pKa = 4.69DD77 pKa = 3.71LPATGNVLTNDD88 pKa = 3.74SDD90 pKa = 4.48PEE92 pKa = 5.26GDD94 pKa = 3.86ALVASLVTAPVNGTVVLNANGSFTYY119 pKa = 10.26IPNANFNGTDD129 pKa = 3.07SLTYY133 pKa = 8.76QVCDD137 pKa = 2.98NGTPSLCDD145 pKa = 2.92TATVVFNVAAINDD158 pKa = 3.78APVAVRR164 pKa = 11.84DD165 pKa = 4.21TVAVTEE171 pKa = 4.61DD172 pKa = 3.44VPATGNVLTNDD183 pKa = 3.74SDD185 pKa = 4.48PEE187 pKa = 5.26GDD189 pKa = 3.86ALVASLVTAPVNGTVVLNANGSFTYY214 pKa = 10.26IPNANFNGTDD224 pKa = 3.07SLTYY228 pKa = 8.76QVCDD232 pKa = 2.98NGTPSLCDD240 pKa = 2.92TATVVFNVAAINDD253 pKa = 3.78APVAVRR259 pKa = 11.84DD260 pKa = 4.21TVAVTEE266 pKa = 4.61DD267 pKa = 3.44VPATGNVLTNDD278 pKa = 3.74SDD280 pKa = 4.48PEE282 pKa = 5.26GDD284 pKa = 3.86ALVASLVTAPVNGTVVLNANGSFTYY309 pKa = 10.1TPNEE313 pKa = 4.05NFNGTDD319 pKa = 2.94SLTYY323 pKa = 8.76QVCDD327 pKa = 2.98NGTPSLCDD335 pKa = 2.92TATVVFNIAAVNDD348 pKa = 3.7APVAVRR354 pKa = 11.84DD355 pKa = 3.97TFQTRR360 pKa = 11.84QTIPVSGNVLTNDD373 pKa = 3.52TDD375 pKa = 4.03PEE377 pKa = 4.36GNALSTNVITGPLHH391 pKa = 5.07GTLVLNADD399 pKa = 3.59GSFIYY404 pKa = 9.97TPNAAFSGIDD414 pKa = 3.13QALYY418 pKa = 9.57RR419 pKa = 11.84VCDD422 pKa = 3.47NGVPSLCDD430 pKa = 2.68TATIVFVVTAVNTAPVAVNDD450 pKa = 3.67SFRR453 pKa = 11.84TVMQVPVSGNVLSNDD468 pKa = 3.58IDD470 pKa = 3.8PQGNGLQVALVDD482 pKa = 3.84NVKK485 pKa = 10.5QGSLQLNADD494 pKa = 3.21GTFRR498 pKa = 11.84YY499 pKa = 9.71EE500 pKa = 3.74PTGTFTGIDD509 pKa = 3.33SFVYY513 pKa = 9.89RR514 pKa = 11.84ICTPGASGLCDD525 pKa = 3.1SATVHH530 pKa = 5.8IQVTGVSEE538 pKa = 4.03QPMIGAALSVSTPVLQPDD556 pKa = 3.78GSYY559 pKa = 10.36RR560 pKa = 11.84VTYY563 pKa = 10.26LLRR566 pKa = 11.84IRR568 pKa = 11.84NYY570 pKa = 10.3GNVALRR576 pKa = 11.84QIEE579 pKa = 4.22ATDD582 pKa = 3.56DD583 pKa = 3.65LSRR586 pKa = 11.84VFPAPAGFRR595 pKa = 11.84VVSITADD602 pKa = 3.15GGLVANGTFDD612 pKa = 3.65GRR614 pKa = 11.84NDD616 pKa = 3.19INLLLSSSEE625 pKa = 3.82LALNVTSAISLVVDD639 pKa = 4.8IIPNGSFGPFNNNVTVQGSSLSGTIVTDD667 pKa = 3.49VSTNGSNPDD676 pKa = 3.34PSGNNDD682 pKa = 3.35PKK684 pKa = 11.32DD685 pKa = 3.72PGEE688 pKa = 4.27NLPTPLVLQPKK699 pKa = 9.76AIAGLAKK706 pKa = 10.44AATAPTAEE714 pKa = 3.69ISGNYY719 pKa = 8.0VVTYY723 pKa = 8.34TFVVKK728 pKa = 10.88NFGNVALNNVQVTDD742 pKa = 4.14DD743 pKa = 3.71LSKK746 pKa = 11.06VFSSPASFQIVGDD759 pKa = 3.4IRR761 pKa = 11.84TTGGLVGNNQYY772 pKa = 11.43NGINNTNLLSAGSGVNAGAADD793 pKa = 4.72TIQVTVRR800 pKa = 11.84IIPNKK805 pKa = 10.11QFGTFNNSAVLTGAAAGMGTTLTDD829 pKa = 4.29LSTNGLNADD838 pKa = 3.72PDD840 pKa = 4.48GNGVPDD846 pKa = 3.95EE847 pKa = 4.18QVVTPLLLNPTRR859 pKa = 11.84LRR861 pKa = 11.84IPEE864 pKa = 4.33GFSPNGDD871 pKa = 3.71GINDD875 pKa = 3.57RR876 pKa = 11.84FIIANAGNDD885 pKa = 3.78KK886 pKa = 10.67IQLEE890 pKa = 4.87VYY892 pKa = 9.8NRR894 pKa = 11.84WGNLVYY900 pKa = 11.02KK901 pKa = 10.21NVNYY905 pKa = 10.32RR906 pKa = 11.84NEE908 pKa = 4.0WDD910 pKa = 3.97GKK912 pKa = 9.69CNTGIHH918 pKa = 6.03IKK920 pKa = 10.58EE921 pKa = 5.09DD922 pKa = 3.7IPDD925 pKa = 3.4GTYY928 pKa = 10.82YY929 pKa = 11.01YY930 pKa = 10.07IVTATGSTGTEE941 pKa = 3.62RR942 pKa = 11.84YY943 pKa = 9.64VNYY946 pKa = 9.04ITIIRR951 pKa = 4.06

Molecular weight: 98.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A249T3H9|A0A249T3H9_9BACT Penicillin-insensitive transglycosylase OS=Chitinophaga sp. MD30 OX=2033437 GN=CK934_27570 PE=4 SV=1
MM1 pKa = 7.56NIITSLTYY9 pKa = 10.76LLPGLLLGFLGSFHH23 pKa = 6.9CVGMCGPIALTLPVIHH39 pKa = 6.91LSSTRR44 pKa = 11.84KK45 pKa = 9.34LAGILLYY52 pKa = 10.72NAGRR56 pKa = 11.84TTTYY60 pKa = 11.13LLLGSLSGWLGQRR73 pKa = 11.84FFIGGLQQWLSILLGILLLLLLCWQYY99 pKa = 11.62ILQKK103 pKa = 9.68PLPQIPFFQQHH114 pKa = 5.07VKK116 pKa = 10.4SALGRR121 pKa = 11.84LLTRR125 pKa = 11.84RR126 pKa = 11.84RR127 pKa = 11.84FSTLFTIGFLNGLLPCGLVYY147 pKa = 10.62IGITGAIATGSAWKK161 pKa = 10.01GALFMTAFGIGTIPAMAAVSWFSQLLRR188 pKa = 11.84VHH190 pKa = 6.66IRR192 pKa = 11.84RR193 pKa = 11.84FIPVAIGMVALLLIVRR209 pKa = 11.84GMNLGIPYY217 pKa = 10.04ISPALKK223 pKa = 9.74EE224 pKa = 4.32KK225 pKa = 10.61KK226 pKa = 10.2VSCCHH231 pKa = 6.42KK232 pKa = 10.7

Molecular weight: 25.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5689

0

5689

2227784

25

13566

391.6

43.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.262 ± 0.043

0.86 ± 0.01

5.206 ± 0.023

5.312 ± 0.037

4.355 ± 0.027

7.019 ± 0.034

2.167 ± 0.02

6.695 ± 0.024

5.509 ± 0.041

9.875 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.27 ± 0.019

4.826 ± 0.041

4.326 ± 0.027

4.552 ± 0.024

4.823 ± 0.023

5.97 ± 0.025

6.061 ± 0.043

6.48 ± 0.03

1.283 ± 0.012

4.149 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski