Amino acid dipepetide frequency for Chitinophaga sp. MD30

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.982AlaAla: 8.982 ± 0.105	0.887AlaCys: 0.887 ± 0.028	4.649AlaAsp: 4.649 ± 0.06	3.951AlaGlu: 3.951 ± 0.051	3.574AlaPhe: 3.574 ± 0.042	6.924AlaGly: 6.924 ± 0.1	1.58AlaHis: 1.58 ± 0.032	5.634AlaIle: 5.634 ± 0.064	3.622AlaLys: 3.622 ± 0.049	7.898AlaLeu: 7.898 ± 0.084	1.828AlaMet: 1.828 ± 0.034	3.584AlaAsn: 3.584 ± 0.058	3.202AlaPro: 3.202 ± 0.048	3.293AlaGln: 3.293 ± 0.042	3.468AlaArg: 3.468 ± 0.04	4.583AlaSer: 4.583 ± 0.055	5.142AlaThr: 5.142 ± 0.089	5.482AlaVal: 5.482 ± 0.065	0.957AlaTrp: 0.957 ± 0.026	3.381AlaTyr: 3.381 ± 0.041	0.0AlaXaa: 0.0 ± 0.0
Cys	0.587CysAla: 0.587 ± 0.021	0.167CysCys: 0.167 ± 0.009	0.403CysAsp: 0.403 ± 0.033	0.405CysGlu: 0.405 ± 0.017	0.427CysPhe: 0.427 ± 0.013	0.643CysGly: 0.643 ± 0.02	0.2CysHis: 0.2 ± 0.011	0.641CysIle: 0.641 ± 0.019	0.45CysLys: 0.45 ± 0.016	0.879CysLeu: 0.879 ± 0.02	0.217CysMet: 0.217 ± 0.01	0.389CysAsn: 0.389 ± 0.017	0.347CysPro: 0.347 ± 0.013	0.288CysGln: 0.288 ± 0.012	0.459CysArg: 0.459 ± 0.019	0.587CysSer: 0.587 ± 0.017	0.486CysThr: 0.486 ± 0.016	0.495CysVal: 0.495 ± 0.017	0.15CysTrp: 0.15 ± 0.009	0.382CysTyr: 0.382 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	4.241AspAla: 4.241 ± 0.05	0.361AspCys: 0.361 ± 0.014	2.275AspAsp: 2.275 ± 0.039	2.854AspGlu: 2.854 ± 0.039	2.448AspPhe: 2.448 ± 0.042	3.744AspGly: 3.744 ± 0.048	1.137AspHis: 1.137 ± 0.023	4.145AspIle: 4.145 ± 0.045	3.272AspLys: 3.272 ± 0.045	4.645AspLeu: 4.645 ± 0.049	1.221AspMet: 1.221 ± 0.027	2.736AspAsn: 2.736 ± 0.033	2.386AspPro: 2.386 ± 0.044	1.93AspGln: 1.93 ± 0.033	2.52AspArg: 2.52 ± 0.035	2.678AspSer: 2.678 ± 0.039	3.05AspThr: 3.05 ± 0.058	3.261AspVal: 3.261 ± 0.044	0.781AspTrp: 0.781 ± 0.019	2.377AspTyr: 2.377 ± 0.034	0.0AspXaa: 0.0 ± 0.0
Glu	4.248GluAla: 4.248 ± 0.06	0.34GluCys: 0.34 ± 0.013	2.632GluAsp: 2.632 ± 0.043	3.783GluGlu: 3.783 ± 0.077	1.738GluPhe: 1.738 ± 0.033	3.406GluGly: 3.406 ± 0.036	1.312GluHis: 1.312 ± 0.029	3.683GluIle: 3.683 ± 0.046	3.772GluLys: 3.772 ± 0.053	5.46GluLeu: 5.46 ± 0.073	1.372GluMet: 1.372 ± 0.028	2.354GluAsn: 2.354 ± 0.036	1.544GluPro: 1.544 ± 0.024	3.17GluGln: 3.17 ± 0.048	2.969GluArg: 2.969 ± 0.044	2.375GluSer: 2.375 ± 0.038	2.605GluThr: 2.605 ± 0.036	3.649GluVal: 3.649 ± 0.05	0.716GluTrp: 0.716 ± 0.019	1.993GluTyr: 1.993 ± 0.037	0.0GluXaa: 0.0 ± 0.0
Phe	2.982PheAla: 2.982 ± 0.048	0.399PheCys: 0.399 ± 0.014	2.706PheAsp: 2.706 ± 0.037	2.36PheGlu: 2.36 ± 0.034	2.2PhePhe: 2.2 ± 0.038	2.939PheGly: 2.939 ± 0.042	0.893PheHis: 0.893 ± 0.021	2.862PheIle: 2.862 ± 0.04	2.22PheLys: 2.22 ± 0.04	3.991PheLeu: 3.991 ± 0.064	1.066PheMet: 1.066 ± 0.023	2.563PheAsn: 2.563 ± 0.047	1.691PhePro: 1.691 ± 0.03	1.445PheGln: 1.445 ± 0.027	2.318PheArg: 2.318 ± 0.037	3.257PheSer: 3.257 ± 0.045	2.88PheThr: 2.88 ± 0.049	2.565PheVal: 2.565 ± 0.035	0.464PheTrp: 0.464 ± 0.016	1.744PheTyr: 1.744 ± 0.033	0.0PheXaa: 0.0 ± 0.0
Gly	5.279GlyAla: 5.279 ± 0.06	0.652GlyCys: 0.652 ± 0.02	3.444GlyAsp: 3.444 ± 0.042	3.346GlyGlu: 3.346 ± 0.046	3.137GlyPhe: 3.137 ± 0.043	5.277GlyGly: 5.277 ± 0.077	1.471GlyHis: 1.471 ± 0.031	5.344GlyIle: 5.344 ± 0.058	4.699GlyLys: 4.699 ± 0.06	6.057GlyLeu: 6.057 ± 0.062	1.854GlyMet: 1.854 ± 0.034	3.771GlyAsn: 3.771 ± 0.056	1.86GlyPro: 1.86 ± 0.034	2.869GlyGln: 2.869 ± 0.039	3.295GlyArg: 3.295 ± 0.048	4.171GlySer: 4.171 ± 0.057	4.49GlyThr: 4.49 ± 0.072	4.808GlyVal: 4.808 ± 0.063	1.071GlyTrp: 1.071 ± 0.025	3.296GlyTyr: 3.296 ± 0.044	0.0GlyXaa: 0.0 ± 0.0
His	1.542HisAla: 1.542 ± 0.031	0.247HisCys: 0.247 ± 0.01	0.882HisAsp: 0.882 ± 0.022	1.018HisGlu: 1.018 ± 0.023	1.221HisPhe: 1.221 ± 0.028	1.2HisGly: 1.2 ± 0.03	0.726HisHis: 0.726 ± 0.02	1.706HisIle: 1.706 ± 0.033	0.961HisLys: 0.961 ± 0.023	2.283HisLeu: 2.283 ± 0.036	0.498HisMet: 0.498 ± 0.014	0.909HisAsn: 0.909 ± 0.02	1.293HisPro: 1.293 ± 0.029	1.045HisGln: 1.045 ± 0.025	1.04HisArg: 1.04 ± 0.027	1.145HisSer: 1.145 ± 0.024	1.424HisThr: 1.424 ± 0.03	1.139HisVal: 1.139 ± 0.025	0.307HisTrp: 0.307 ± 0.011	1.082HisTyr: 1.082 ± 0.024	0.0HisXaa: 0.0 ± 0.0
Ile	5.943IleAla: 5.943 ± 0.064	0.717IleCys: 0.717 ± 0.02	3.716IleAsp: 3.716 ± 0.038	3.186IleGlu: 3.186 ± 0.046	2.577IlePhe: 2.577 ± 0.05	4.532IleGly: 4.532 ± 0.051	1.437IleHis: 1.437 ± 0.03	4.225IleIle: 4.225 ± 0.057	3.474IleLys: 3.474 ± 0.053	5.861IleLeu: 5.861 ± 0.065	1.258IleMet: 1.258 ± 0.031	3.509IleAsn: 3.509 ± 0.054	3.416IlePro: 3.416 ± 0.041	2.604IleGln: 2.604 ± 0.034	3.831IleArg: 3.831 ± 0.045	4.743IleSer: 4.743 ± 0.048	4.641IleThr: 4.641 ± 0.06	3.999IleVal: 3.999 ± 0.053	0.759IleTrp: 0.759 ± 0.023	2.522IleTyr: 2.522 ± 0.036	0.0IleXaa: 0.0 ± 0.0
Lys	4.535LysAla: 4.535 ± 0.053	0.277LysCys: 0.277 ± 0.012	3.49LysAsp: 3.49 ± 0.049	3.97LysGlu: 3.97 ± 0.049	1.707LysPhe: 1.707 ± 0.029	4.19LysGly: 4.19 ± 0.05	1.121LysHis: 1.121 ± 0.021	3.476LysIle: 3.476 ± 0.046	3.671LysLys: 3.671 ± 0.063	4.653LysLeu: 4.653 ± 0.058	1.457LysMet: 1.457 ± 0.032	2.517LysAsn: 2.517 ± 0.05	2.147LysPro: 2.147 ± 0.035	2.704LysGln: 2.704 ± 0.037	2.534LysArg: 2.534 ± 0.039	2.751LysSer: 2.751 ± 0.036	3.146LysThr: 3.146 ± 0.045	3.845LysVal: 3.845 ± 0.05	0.722LysTrp: 0.722 ± 0.019	2.18LysTyr: 2.18 ± 0.041	0.0LysXaa: 0.0 ± 0.0
Leu	7.441LeuAla: 7.441 ± 0.08	0.978LeuCys: 0.978 ± 0.022	4.849LeuAsp: 4.849 ± 0.058	5.154LeuGlu: 5.154 ± 0.071	4.384LeuPhe: 4.384 ± 0.054	5.794LeuGly: 5.794 ± 0.064	2.287LeuHis: 2.287 ± 0.043	5.538LeuIle: 5.538 ± 0.068	5.568LeuLys: 5.568 ± 0.057	11.565LeuLeu: 11.565 ± 0.128	2.044LeuMet: 2.044 ± 0.038	4.429LeuAsn: 4.429 ± 0.052	5.231LeuPro: 5.231 ± 0.066	5.488LeuGln: 5.488 ± 0.072	4.89LeuArg: 4.89 ± 0.055	7.293LeuSer: 7.293 ± 0.082	5.645LeuThr: 5.645 ± 0.066	5.597LeuVal: 5.597 ± 0.061	0.99LeuTrp: 0.99 ± 0.023	3.582LeuTyr: 3.582 ± 0.037	0.0LeuXaa: 0.0 ± 0.0
Met	1.823MetAla: 1.823 ± 0.031	0.149MetCys: 0.149 ± 0.008	1.254MetAsp: 1.254 ± 0.022	1.434MetGlu: 1.434 ± 0.025	0.741MetPhe: 0.741 ± 0.023	1.477MetGly: 1.477 ± 0.029	0.525MetHis: 0.525 ± 0.016	1.453MetIle: 1.453 ± 0.026	1.663MetLys: 1.663 ± 0.035	2.252MetLeu: 2.252 ± 0.037	0.594MetMet: 0.594 ± 0.018	1.093MetAsn: 1.093 ± 0.029	1.155MetPro: 1.155 ± 0.026	1.229MetGln: 1.229 ± 0.022	1.122MetArg: 1.122 ± 0.026	1.344MetSer: 1.344 ± 0.026	1.18MetThr: 1.18 ± 0.026	1.35MetVal: 1.35 ± 0.028	0.193MetTrp: 0.193 ± 0.008	0.664MetTyr: 0.664 ± 0.019	0.0MetXaa: 0.0 ± 0.0
Asn	3.849AsnAla: 3.849 ± 0.054	0.371AsnCys: 0.371 ± 0.025	2.364AsnAsp: 2.364 ± 0.051	2.39AsnGlu: 2.39 ± 0.043	1.976AsnPhe: 1.976 ± 0.034	3.972AsnGly: 3.972 ± 0.071	0.787AsnHis: 0.787 ± 0.02	3.872AsnIle: 3.872 ± 0.051	2.744AsnLys: 2.744 ± 0.044	3.837AsnLeu: 3.837 ± 0.058	1.059AsnMet: 1.059 ± 0.023	2.978AsnAsn: 2.978 ± 0.055	2.284AsnPro: 2.284 ± 0.038	1.668AsnGln: 1.668 ± 0.03	2.295AsnArg: 2.295 ± 0.036	2.564AsnSer: 2.564 ± 0.041	3.503AsnThr: 3.503 ± 0.055	2.936AsnVal: 2.936 ± 0.053	0.668AsnTrp: 0.668 ± 0.02	2.147AsnTyr: 2.147 ± 0.038	0.0AsnXaa: 0.0 ± 0.0
Pro	5.019ProAla: 5.019 ± 0.098	0.27ProCys: 0.27 ± 0.013	2.837ProAsp: 2.837 ± 0.037	2.713ProGlu: 2.713 ± 0.037	2.051ProPhe: 2.051 ± 0.031	3.502ProGly: 3.502 ± 0.038	0.807ProHis: 0.807 ± 0.022	2.327ProIle: 2.327 ± 0.037	1.75ProLys: 1.75 ± 0.032	4.206ProLeu: 4.206 ± 0.06	0.807ProMet: 0.807 ± 0.019	1.686ProAsn: 1.686 ± 0.033	1.484ProPro: 1.484 ± 0.038	1.62ProGln: 1.62 ± 0.031	1.497ProArg: 1.497 ± 0.027	2.423ProSer: 2.423 ± 0.044	2.178ProThr: 2.178 ± 0.039	4.035ProVal: 4.035 ± 0.064	0.455ProTrp: 0.455 ± 0.016	1.589ProTyr: 1.589 ± 0.031	0.0ProXaa: 0.0 ± 0.0
Gln	3.367GlnAla: 3.367 ± 0.052	0.267GlnCys: 0.267 ± 0.012	1.981GlnAsp: 1.981 ± 0.033	2.737GlnGlu: 2.737 ± 0.043	1.719GlnPhe: 1.719 ± 0.035	2.541GlnGly: 2.541 ± 0.037	1.371GlnHis: 1.371 ± 0.028	2.365GlnIle: 2.365 ± 0.034	1.926GlnLys: 1.926 ± 0.034	5.839GlnLeu: 5.839 ± 0.073	0.923GlnMet: 0.923 ± 0.022	1.428GlnAsn: 1.428 ± 0.029	2.214GlnPro: 2.214 ± 0.034	3.922GlnGln: 3.922 ± 0.064	2.467GlnArg: 2.467 ± 0.034	2.279GlnSer: 2.279 ± 0.034	2.272GlnThr: 2.272 ± 0.035	3.271GlnVal: 3.271 ± 0.047	0.591GlnTrp: 0.591 ± 0.016	2.039GlnTyr: 2.039 ± 0.034	0.0GlnXaa: 0.0 ± 0.0
Arg	2.867ArgAla: 2.867 ± 0.037	0.328ArgCys: 0.328 ± 0.012	2.298ArgAsp: 2.298 ± 0.029	2.844ArgGlu: 2.844 ± 0.045	2.381ArgPhe: 2.381 ± 0.04	2.435ArgGly: 2.435 ± 0.035	1.189ArgHis: 1.189 ± 0.025	3.74ArgIle: 3.74 ± 0.043	2.969ArgLys: 2.969 ± 0.043	5.075ArgLeu: 5.075 ± 0.065	1.332ArgMet: 1.332 ± 0.03	2.499ArgAsn: 2.499 ± 0.039	1.911ArgPro: 1.911 ± 0.035	2.621ArgGln: 2.621 ± 0.036	2.3ArgArg: 2.3 ± 0.038	2.794ArgSer: 2.794 ± 0.038	2.709ArgThr: 2.709 ± 0.032	2.755ArgVal: 2.755 ± 0.042	0.784ArgTrp: 0.784 ± 0.018	2.403ArgTyr: 2.403 ± 0.038	0.0ArgXaa: 0.0 ± 0.0
Ser	4.9SerAla: 4.9 ± 0.057	0.562SerCys: 0.562 ± 0.017	2.792SerAsp: 2.792 ± 0.035	2.495SerGlu: 2.495 ± 0.039	3.208SerPhe: 3.208 ± 0.045	4.977SerGly: 4.977 ± 0.066	1.175SerHis: 1.175 ± 0.026	4.005SerIle: 4.005 ± 0.048	2.915SerLys: 2.915 ± 0.041	6.352SerLeu: 6.352 ± 0.062	1.303SerMet: 1.303 ± 0.027	2.8SerAsn: 2.8 ± 0.05	2.502SerPro: 2.502 ± 0.036	2.147SerGln: 2.147 ± 0.034	2.918SerArg: 2.918 ± 0.042	3.699SerSer: 3.699 ± 0.048	3.475SerThr: 3.475 ± 0.047	3.86SerVal: 3.86 ± 0.045	0.899SerTrp: 0.899 ± 0.02	2.713SerTyr: 2.713 ± 0.041	0.0SerXaa: 0.0 ± 0.0
Thr	5.893ThrAla: 5.893 ± 0.097	0.439ThrCys: 0.439 ± 0.015	3.321ThrAsp: 3.321 ± 0.041	2.587ThrGlu: 2.587 ± 0.04	2.651ThrPhe: 2.651 ± 0.043	5.321ThrGly: 5.321 ± 0.083	1.148ThrHis: 1.148 ± 0.028	4.128ThrIle: 4.128 ± 0.056	2.441ThrLys: 2.441 ± 0.035	6.0ThrLeu: 6.0 ± 0.064	1.068ThrMet: 1.068 ± 0.024	2.601ThrAsn: 2.601 ± 0.044	3.255ThrPro: 3.255 ± 0.059	2.129ThrGln: 2.129 ± 0.03	2.534ThrArg: 2.534 ± 0.035	3.483ThrSer: 3.483 ± 0.045	4.079ThrThr: 4.079 ± 0.068	4.412ThrVal: 4.412 ± 0.084	0.711ThrTrp: 0.711 ± 0.018	2.414ThrTyr: 2.414 ± 0.041	0.0ThrXaa: 0.0 ± 0.0
Val	5.226ValAla: 5.226 ± 0.059	0.633ValCys: 0.633 ± 0.02	3.256ValAsp: 3.256 ± 0.042	3.061ValGlu: 3.061 ± 0.041	2.894ValPhe: 2.894 ± 0.044	3.702ValGly: 3.702 ± 0.057	1.262ValHis: 1.262 ± 0.028	4.563ValIle: 4.563 ± 0.051	4.01ValLys: 4.01 ± 0.04	6.393ValLeu: 6.393 ± 0.069	1.545ValMet: 1.545 ± 0.033	3.442ValAsn: 3.442 ± 0.052	3.02ValPro: 3.02 ± 0.048	2.631ValGln: 2.631 ± 0.039	2.88ValArg: 2.88 ± 0.046	4.407ValSer: 4.407 ± 0.056	4.267ValThr: 4.267 ± 0.076	4.318ValVal: 4.318 ± 0.056	0.729ValTrp: 0.729 ± 0.021	2.56ValTyr: 2.56 ± 0.035	0.0ValXaa: 0.0 ± 0.0
Trp	0.804TrpAla: 0.804 ± 0.024	0.137TrpCys: 0.137 ± 0.008	0.615TrpAsp: 0.615 ± 0.017	0.803TrpGlu: 0.803 ± 0.022	0.529TrpPhe: 0.529 ± 0.016	0.83TrpGly: 0.83 ± 0.022	0.313TrpHis: 0.313 ± 0.013	0.763TrpIle: 0.763 ± 0.019	0.808TrpLys: 0.808 ± 0.02	1.41TrpLeu: 1.41 ± 0.028	0.373TrpMet: 0.373 ± 0.014	0.663TrpAsn: 0.663 ± 0.02	0.399TrpPro: 0.399 ± 0.016	0.873TrpGln: 0.873 ± 0.024	0.624TrpArg: 0.624 ± 0.018	0.764TrpSer: 0.764 ± 0.023	0.675TrpThr: 0.675 ± 0.021	0.672TrpVal: 0.672 ± 0.019	0.249TrpTrp: 0.249 ± 0.012	0.526TrpTyr: 0.526 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.093TyrAla: 3.093 ± 0.041	0.421TyrCys: 0.421 ± 0.014	2.296TyrAsp: 2.296 ± 0.043	2.03TyrGlu: 2.03 ± 0.034	1.983TyrPhe: 1.983 ± 0.038	2.785TyrGly: 2.785 ± 0.045	0.927TyrHis: 0.927 ± 0.021	2.481TyrIle: 2.481 ± 0.036	2.155TyrLys: 2.155 ± 0.036	4.088TyrLeu: 4.088 ± 0.048	0.878TyrMet: 0.878 ± 0.02	2.313TyrAsn: 2.313 ± 0.043	1.915TyrPro: 1.915 ± 0.036	1.844TyrGln: 1.844 ± 0.029	2.272TyrArg: 2.272 ± 0.034	2.359TyrSer: 2.359 ± 0.04	2.767TyrThr: 2.767 ± 0.038	2.35TyrVal: 2.35 ± 0.034	0.637TyrTrp: 0.637 ± 0.018	1.896TyrTyr: 1.896 ± 0.036	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5689 proteins (2227784 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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