Rhodopseudomonas faecalis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Bradyrhizobiaceae; Rhodopseudomonas

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3711 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A318TH66|A0A318TH66_9BRAD Protoporphyrinogen IX oxidase OS=Rhodopseudomonas faecalis OX=99655 GN=BJ122_112100 PE=3 SV=1
MM1 pKa = 7.64SYY3 pKa = 11.71ADD5 pKa = 3.5ATAFAASLAATLMVEE20 pKa = 3.94IVVFQAGDD28 pKa = 3.69GTHH31 pKa = 6.95AACPAAEE38 pKa = 4.28FDD40 pKa = 4.86GDD42 pKa = 3.91DD43 pKa = 4.91DD44 pKa = 4.21MVKK47 pKa = 10.82LEE49 pKa = 4.52LDD51 pKa = 3.1PWGG54 pKa = 3.85

Molecular weight: 5.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A318T9C7|A0A318T9C7_9BRAD Tight adherence protein B OS=Rhodopseudomonas faecalis OX=99655 GN=BJ122_11852 PE=4 SV=1
MM1 pKa = 6.42TTALSSVLLPIALAAVAVVLLLGLINMMRR30 pKa = 11.84GGSPNRR36 pKa = 11.84SQRR39 pKa = 11.84LMRR42 pKa = 11.84LRR44 pKa = 11.84VLLQLVAIAVTMAAVWAIGRR64 pKa = 4.0

Molecular weight: 6.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3711

0

3711

1149817

26

2111

309.8

33.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.931 ± 0.054

0.869 ± 0.013

5.614 ± 0.031

5.415 ± 0.037

3.571 ± 0.028

8.117 ± 0.039

2.016 ± 0.02

5.237 ± 0.03

3.451 ± 0.034

10.225 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.348 ± 0.018

2.599 ± 0.022

5.342 ± 0.036

3.49 ± 0.025

7.251 ± 0.036

5.549 ± 0.034

5.191 ± 0.029

7.367 ± 0.038

1.255 ± 0.016

2.162 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski