Amino acid dipepetide frequency for Rhodopseudomonas faecalis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	19.673AlaAla: 19.673 ± 0.211	1.073AlaCys: 1.073 ± 0.032	7.002AlaAsp: 7.002 ± 0.081	7.682AlaGlu: 7.682 ± 0.104	4.161AlaPhe: 4.161 ± 0.074	9.831AlaGly: 9.831 ± 0.107	2.195AlaHis: 2.195 ± 0.048	6.751AlaIle: 6.751 ± 0.081	4.903AlaLys: 4.903 ± 0.101	13.928AlaLeu: 13.928 ± 0.142	3.477AlaMet: 3.477 ± 0.056	2.986AlaAsn: 2.986 ± 0.052	6.281AlaPro: 6.281 ± 0.094	4.46AlaGln: 4.46 ± 0.073	8.855AlaArg: 8.855 ± 0.109	6.819AlaSer: 6.819 ± 0.082	6.303AlaThr: 6.303 ± 0.086	9.147AlaVal: 9.147 ± 0.095	1.375AlaTrp: 1.375 ± 0.036	2.41AlaTyr: 2.41 ± 0.043	0.0AlaXaa: 0.0 ± 0.0
Cys	1.026CysAla: 1.026 ± 0.031	0.134CysCys: 0.134 ± 0.013	0.525CysAsp: 0.525 ± 0.021	0.458CysGlu: 0.458 ± 0.019	0.286CysPhe: 0.286 ± 0.016	0.956CysGly: 0.956 ± 0.036	0.212CysHis: 0.212 ± 0.014	0.391CysIle: 0.391 ± 0.018	0.213CysLys: 0.213 ± 0.015	0.731CysLeu: 0.731 ± 0.028	0.17CysMet: 0.17 ± 0.013	0.242CysAsn: 0.242 ± 0.013	0.481CysPro: 0.481 ± 0.024	0.27CysGln: 0.27 ± 0.017	0.67CysArg: 0.67 ± 0.025	0.547CysSer: 0.547 ± 0.022	0.424CysThr: 0.424 ± 0.021	0.628CysVal: 0.628 ± 0.023	0.11CysTrp: 0.11 ± 0.01	0.217CysTyr: 0.217 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	6.739AspAla: 6.739 ± 0.07	0.491AspCys: 0.491 ± 0.024	3.489AspAsp: 3.489 ± 0.067	3.276AspGlu: 3.276 ± 0.057	2.011AspPhe: 2.011 ± 0.04	4.898AspGly: 4.898 ± 0.068	1.368AspHis: 1.368 ± 0.038	2.827AspIle: 2.827 ± 0.05	1.915AspLys: 1.915 ± 0.047	5.664AspLeu: 5.664 ± 0.07	1.158AspMet: 1.158 ± 0.03	1.44AspAsn: 1.44 ± 0.036	3.33AspPro: 3.33 ± 0.056	2.136AspGln: 2.136 ± 0.044	4.263AspArg: 4.263 ± 0.066	2.381AspSer: 2.381 ± 0.047	2.493AspThr: 2.493 ± 0.046	3.877AspVal: 3.877 ± 0.056	0.855AspTrp: 0.855 ± 0.027	1.53AspTyr: 1.53 ± 0.039	0.0AspXaa: 0.0 ± 0.0
Glu	7.245GluAla: 7.245 ± 0.098	0.37GluCys: 0.37 ± 0.018	2.315GluAsp: 2.315 ± 0.05	2.553GluGlu: 2.553 ± 0.059	1.833GluPhe: 1.833 ± 0.044	3.59GluGly: 3.59 ± 0.06	1.157GluHis: 1.157 ± 0.033	3.541GluIle: 3.541 ± 0.07	1.875GluLys: 1.875 ± 0.045	5.913GluLeu: 5.913 ± 0.076	1.345GluMet: 1.345 ± 0.034	1.28GluAsn: 1.28 ± 0.033	2.849GluPro: 2.849 ± 0.066	2.596GluGln: 2.596 ± 0.058	5.105GluArg: 5.105 ± 0.077	2.206GluSer: 2.206 ± 0.05	2.997GluThr: 2.997 ± 0.055	3.906GluVal: 3.906 ± 0.059	0.609GluTrp: 0.609 ± 0.02	0.865GluTyr: 0.865 ± 0.027	0.0GluXaa: 0.0 ± 0.0
Phe	4.789PheAla: 4.789 ± 0.071	0.42PheCys: 0.42 ± 0.018	2.519PheAsp: 2.519 ± 0.043	2.062PheGlu: 2.062 ± 0.04	1.303PhePhe: 1.303 ± 0.034	3.68PheGly: 3.68 ± 0.066	0.673PheHis: 0.673 ± 0.029	1.647PheIle: 1.647 ± 0.04	1.094PheLys: 1.094 ± 0.039	3.063PheLeu: 3.063 ± 0.057	0.741PheMet: 0.741 ± 0.025	1.072PheAsn: 1.072 ± 0.03	1.449PhePro: 1.449 ± 0.035	0.963PheGln: 0.963 ± 0.028	2.239PheArg: 2.239 ± 0.043	2.074PheSer: 2.074 ± 0.043	1.879PheThr: 1.879 ± 0.042	2.759PheVal: 2.759 ± 0.052	0.481PheTrp: 0.481 ± 0.019	0.802PheTyr: 0.802 ± 0.032	0.0PheXaa: 0.0 ± 0.0
Gly	9.208GlyAla: 9.208 ± 0.106	0.895GlyCys: 0.895 ± 0.025	4.29GlyAsp: 4.29 ± 0.067	4.462GlyGlu: 4.462 ± 0.072	3.514GlyPhe: 3.514 ± 0.061	6.91GlyGly: 6.91 ± 0.121	1.775GlyHis: 1.775 ± 0.043	4.205GlyIle: 4.205 ± 0.063	3.104GlyLys: 3.104 ± 0.06	8.415GlyLeu: 8.415 ± 0.095	1.932GlyMet: 1.932 ± 0.045	2.077GlyAsn: 2.077 ± 0.051	3.284GlyPro: 3.284 ± 0.056	2.813GlyGln: 2.813 ± 0.053	6.022GlyArg: 6.022 ± 0.082	4.605GlySer: 4.605 ± 0.075	4.192GlyThr: 4.192 ± 0.072	6.002GlyVal: 6.002 ± 0.075	1.225GlyTrp: 1.225 ± 0.031	2.239GlyTyr: 2.239 ± 0.048	0.0GlyXaa: 0.0 ± 0.0
His	2.259HisAla: 2.259 ± 0.044	0.24HisCys: 0.24 ± 0.015	1.318HisAsp: 1.318 ± 0.04	0.978HisGlu: 0.978 ± 0.033	0.811HisPhe: 0.811 ± 0.026	2.072HisGly: 2.072 ± 0.054	0.645HisHis: 0.645 ± 0.028	0.851HisIle: 0.851 ± 0.029	0.512HisLys: 0.512 ± 0.022	1.986HisLeu: 1.986 ± 0.041	0.409HisMet: 0.409 ± 0.02	0.484HisAsn: 0.484 ± 0.019	1.35HisPro: 1.35 ± 0.035	0.662HisGln: 0.662 ± 0.025	1.577HisArg: 1.577 ± 0.039	1.011HisSer: 1.011 ± 0.029	0.778HisThr: 0.778 ± 0.026	1.352HisVal: 1.352 ± 0.034	0.323HisTrp: 0.323 ± 0.016	0.55HisTyr: 0.55 ± 0.023	0.0HisXaa: 0.0 ± 0.0
Ile	8.104IleAla: 8.104 ± 0.096	0.465IleCys: 0.465 ± 0.02	3.995IleAsp: 3.995 ± 0.067	3.795IleGlu: 3.795 ± 0.057	1.417IlePhe: 1.417 ± 0.045	5.325IleGly: 5.325 ± 0.078	0.858IleHis: 0.858 ± 0.027	2.253IleIle: 2.253 ± 0.043	1.827IleLys: 1.827 ± 0.046	3.746IleLeu: 3.746 ± 0.061	0.899IleMet: 0.899 ± 0.025	1.617IleAsn: 1.617 ± 0.045	2.18IlePro: 2.18 ± 0.046	1.155IleGln: 1.155 ± 0.032	3.066IleArg: 3.066 ± 0.052	2.887IleSer: 2.887 ± 0.054	2.832IleThr: 2.832 ± 0.054	4.243IleVal: 4.243 ± 0.064	0.589IleTrp: 0.589 ± 0.022	1.113IleTyr: 1.113 ± 0.035	0.0IleXaa: 0.0 ± 0.0
Lys	4.354LysAla: 4.354 ± 0.093	0.179LysCys: 0.179 ± 0.012	1.701LysAsp: 1.701 ± 0.044	1.602LysGlu: 1.602 ± 0.044	0.998LysPhe: 0.998 ± 0.033	2.418LysGly: 2.418 ± 0.049	0.685LysHis: 0.685 ± 0.025	1.879LysIle: 1.879 ± 0.043	1.467LysLys: 1.467 ± 0.055	3.872LysLeu: 3.872 ± 0.07	0.86LysMet: 0.86 ± 0.025	0.906LysAsn: 0.906 ± 0.03	2.323LysPro: 2.323 ± 0.061	1.217LysGln: 1.217 ± 0.038	2.498LysArg: 2.498 ± 0.047	1.962LysSer: 1.962 ± 0.05	2.025LysThr: 2.025 ± 0.047	2.531LysVal: 2.531 ± 0.053	0.352LysTrp: 0.352 ± 0.016	0.678LysTyr: 0.678 ± 0.025	0.0LysXaa: 0.0 ± 0.0
Leu	13.844LeuAla: 13.844 ± 0.136	0.904LeuCys: 0.904 ± 0.028	5.784LeuAsp: 5.784 ± 0.06	4.837LeuGlu: 4.837 ± 0.071	3.639LeuPhe: 3.639 ± 0.067	7.987LeuGly: 7.987 ± 0.093	1.923LeuHis: 1.923 ± 0.047	5.416LeuIle: 5.416 ± 0.071	3.673LeuLys: 3.673 ± 0.065	10.386LeuLeu: 10.386 ± 0.133	2.32LeuMet: 2.32 ± 0.049	2.534LeuAsn: 2.534 ± 0.049	5.684LeuPro: 5.684 ± 0.074	3.327LeuGln: 3.327 ± 0.057	7.368LeuArg: 7.368 ± 0.103	6.37LeuSer: 6.37 ± 0.077	5.79LeuThr: 5.79 ± 0.068	7.32LeuVal: 7.32 ± 0.082	1.153LeuTrp: 1.153 ± 0.036	1.992LeuTyr: 1.992 ± 0.04	0.0LeuXaa: 0.0 ± 0.0
Met	2.93MetAla: 2.93 ± 0.052	0.151MetCys: 0.151 ± 0.011	0.891MetAsp: 0.891 ± 0.029	0.92MetGlu: 0.92 ± 0.031	0.694MetPhe: 0.694 ± 0.028	1.386MetGly: 1.386 ± 0.036	0.423MetHis: 0.423 ± 0.021	1.421MetIle: 1.421 ± 0.034	0.9MetLys: 0.9 ± 0.03	2.494MetLeu: 2.494 ± 0.051	0.675MetMet: 0.675 ± 0.024	0.683MetAsn: 0.683 ± 0.028	1.522MetPro: 1.522 ± 0.036	0.869MetGln: 0.869 ± 0.028	1.739MetArg: 1.739 ± 0.038	1.635MetSer: 1.635 ± 0.037	1.848MetThr: 1.848 ± 0.043	1.746MetVal: 1.746 ± 0.038	0.222MetTrp: 0.222 ± 0.013	0.324MetTyr: 0.324 ± 0.018	0.0MetXaa: 0.0 ± 0.0
Asn	3.256AsnAla: 3.256 ± 0.056	0.244AsnCys: 0.244 ± 0.015	1.476AsnAsp: 1.476 ± 0.038	1.299AsnGlu: 1.299 ± 0.036	0.937AsnPhe: 0.937 ± 0.029	2.307AsnGly: 2.307 ± 0.052	0.521AsnHis: 0.521 ± 0.022	1.302AsnIle: 1.302 ± 0.036	0.847AsnLys: 0.847 ± 0.026	2.489AsnLeu: 2.489 ± 0.05	0.584AsnMet: 0.584 ± 0.023	0.764AsnAsn: 0.764 ± 0.031	1.681AsnPro: 1.681 ± 0.035	0.813AsnGln: 0.813 ± 0.027	1.779AsnArg: 1.779 ± 0.037	1.316AsnSer: 1.316 ± 0.038	1.158AsnThr: 1.158 ± 0.031	2.046AsnVal: 2.046 ± 0.045	0.444AsnTrp: 0.444 ± 0.023	0.729AsnTyr: 0.729 ± 0.027	0.0AsnXaa: 0.0 ± 0.0
Pro	6.721ProAla: 6.721 ± 0.098	0.379ProCys: 0.379 ± 0.021	3.454ProAsp: 3.454 ± 0.058	3.448ProGlu: 3.448 ± 0.061	1.915ProPhe: 1.915 ± 0.047	4.302ProGly: 4.302 ± 0.072	1.071ProHis: 1.071 ± 0.029	2.397ProIle: 2.397 ± 0.045	1.832ProLys: 1.832 ± 0.047	4.926ProLeu: 4.926 ± 0.075	1.188ProMet: 1.188 ± 0.038	1.429ProAsn: 1.429 ± 0.04	3.24ProPro: 3.24 ± 0.078	2.059ProGln: 2.059 ± 0.043	3.369ProArg: 3.369 ± 0.069	3.187ProSer: 3.187 ± 0.057	2.616ProThr: 2.616 ± 0.046	4.051ProVal: 4.051 ± 0.068	0.714ProTrp: 0.714 ± 0.027	1.119ProTyr: 1.119 ± 0.029	0.0ProXaa: 0.0 ± 0.0
Gln	4.4GlnAla: 4.4 ± 0.076	0.278GlnCys: 0.278 ± 0.016	1.516GlnAsp: 1.516 ± 0.039	1.389GlnGlu: 1.389 ± 0.036	1.213GlnPhe: 1.213 ± 0.031	2.401GlnGly: 2.401 ± 0.047	0.774GlnHis: 0.774 ± 0.029	2.033GlnIle: 2.033 ± 0.05	1.013GlnLys: 1.013 ± 0.031	3.684GlnLeu: 3.684 ± 0.058	0.866GlnMet: 0.866 ± 0.03	0.922GlnAsn: 0.922 ± 0.032	2.278GlnPro: 2.278 ± 0.054	1.765GlnGln: 1.765 ± 0.053	3.123GlnArg: 3.123 ± 0.052	2.052GlnSer: 2.052 ± 0.042	1.724GlnThr: 1.724 ± 0.042	2.451GlnVal: 2.451 ± 0.045	0.445GlnTrp: 0.445 ± 0.02	0.572GlnTyr: 0.572 ± 0.022	0.0GlnXaa: 0.0 ± 0.0
Arg	8.574ArgAla: 8.574 ± 0.085	0.567ArgCys: 0.567 ± 0.026	4.347ArgAsp: 4.347 ± 0.072	4.127ArgGlu: 4.127 ± 0.071	2.915ArgPhe: 2.915 ± 0.054	5.125ArgGly: 5.125 ± 0.078	1.678ArgHis: 1.678 ± 0.042	3.935ArgIle: 3.935 ± 0.054	2.459ArgLys: 2.459 ± 0.052	8.024ArgLeu: 8.024 ± 0.104	1.785ArgMet: 1.785 ± 0.039	1.922ArgAsn: 1.922 ± 0.041	3.703ArgPro: 3.703 ± 0.074	2.794ArgGln: 2.794 ± 0.051	5.885ArgArg: 5.885 ± 0.085	3.962ArgSer: 3.962 ± 0.058	3.134ArgThr: 3.134 ± 0.047	4.8ArgVal: 4.8 ± 0.073	1.018ArgTrp: 1.018 ± 0.033	1.752ArgTyr: 1.752 ± 0.043	0.0ArgXaa: 0.0 ± 0.0
Ser	6.85SerAla: 6.85 ± 0.09	0.487SerCys: 0.487 ± 0.021	3.092SerAsp: 3.092 ± 0.057	2.878SerGlu: 2.878 ± 0.056	2.251SerPhe: 2.251 ± 0.045	5.276SerGly: 5.276 ± 0.078	1.105SerHis: 1.105 ± 0.031	2.807SerIle: 2.807 ± 0.052	1.836SerLys: 1.836 ± 0.041	5.318SerLeu: 5.318 ± 0.071	1.259SerMet: 1.259 ± 0.032	1.53SerAsn: 1.53 ± 0.034	2.852SerPro: 2.852 ± 0.049	1.732SerGln: 1.732 ± 0.042	3.848SerArg: 3.848 ± 0.059	3.456SerSer: 3.456 ± 0.069	2.791SerThr: 2.791 ± 0.055	3.933SerVal: 3.933 ± 0.066	0.758SerTrp: 0.758 ± 0.024	1.431SerTyr: 1.431 ± 0.035	0.0SerXaa: 0.0 ± 0.0
Thr	6.371ThrAla: 6.371 ± 0.076	0.431ThrCys: 0.431 ± 0.018	2.572ThrAsp: 2.572 ± 0.052	2.727ThrGlu: 2.727 ± 0.046	1.824ThrPhe: 1.824 ± 0.04	4.603ThrGly: 4.603 ± 0.079	0.903ThrHis: 0.903 ± 0.032	2.983ThrIle: 2.983 ± 0.057	1.698ThrLys: 1.698 ± 0.041	5.877ThrLeu: 5.877 ± 0.074	1.207ThrMet: 1.207 ± 0.032	1.231ThrAsn: 1.231 ± 0.034	3.271ThrPro: 3.271 ± 0.059	1.409ThrGln: 1.409 ± 0.038	3.175ThrArg: 3.175 ± 0.059	2.874ThrSer: 2.874 ± 0.054	2.959ThrThr: 2.959 ± 0.059	4.258ThrVal: 4.258 ± 0.065	0.543ThrTrp: 0.543 ± 0.022	0.991ThrTyr: 0.991 ± 0.035	0.0ThrXaa: 0.0 ± 0.0
Val	9.356ValAla: 9.356 ± 0.1	0.609ValCys: 0.609 ± 0.024	3.946ValAsp: 3.946 ± 0.054	4.184ValGlu: 4.184 ± 0.069	2.576ValPhe: 2.576 ± 0.053	5.282ValGly: 5.282 ± 0.068	1.378ValHis: 1.378 ± 0.039	4.241ValIle: 4.241 ± 0.068	2.353ValLys: 2.353 ± 0.05	7.715ValLeu: 7.715 ± 0.088	1.898ValMet: 1.898 ± 0.045	1.915ValAsn: 1.915 ± 0.04	3.954ValPro: 3.954 ± 0.064	2.296ValGln: 2.296 ± 0.048	4.883ValArg: 4.883 ± 0.068	4.262ValSer: 4.262 ± 0.061	4.337ValThr: 4.337 ± 0.069	6.259ValVal: 6.259 ± 0.088	0.8ValTrp: 0.8 ± 0.026	1.432ValTyr: 1.432 ± 0.038	0.0ValXaa: 0.0 ± 0.0
Trp	1.103TrpAla: 1.103 ± 0.029	0.131TrpCys: 0.131 ± 0.011	0.541TrpAsp: 0.541 ± 0.022	0.448TrpGlu: 0.448 ± 0.02	0.487TrpPhe: 0.487 ± 0.018	0.778TrpGly: 0.778 ± 0.026	0.333TrpHis: 0.333 ± 0.018	0.625TrpIle: 0.625 ± 0.022	0.382TrpLys: 0.382 ± 0.019	1.726TrpLeu: 1.726 ± 0.043	0.33TrpMet: 0.33 ± 0.019	0.415TrpAsn: 0.415 ± 0.021	0.699TrpPro: 0.699 ± 0.028	0.636TrpGln: 0.636 ± 0.025	1.213TrpArg: 1.213 ± 0.034	0.797TrpSer: 0.797 ± 0.03	0.697TrpThr: 0.697 ± 0.029	0.755TrpVal: 0.755 ± 0.026	0.214TrpTrp: 0.214 ± 0.014	0.245TrpTyr: 0.245 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.509TyrAla: 2.509 ± 0.047	0.24TyrCys: 0.24 ± 0.015	1.371TyrAsp: 1.371 ± 0.035	1.026TyrGlu: 1.026 ± 0.032	0.924TyrPhe: 0.924 ± 0.031	2.042TyrGly: 2.042 ± 0.04	0.488TyrHis: 0.488 ± 0.022	0.863TyrIle: 0.863 ± 0.024	0.604TyrLys: 0.604 ± 0.022	2.293TyrLeu: 2.293 ± 0.049	0.372TyrMet: 0.372 ± 0.019	0.545TyrAsn: 0.545 ± 0.026	1.005TyrPro: 1.005 ± 0.027	0.931TyrGln: 0.931 ± 0.035	1.827TyrArg: 1.827 ± 0.043	1.087TyrSer: 1.087 ± 0.039	0.931TyrThr: 0.931 ± 0.03	1.61TyrVal: 1.61 ± 0.039	0.324TyrTrp: 0.324 ± 0.018	0.63TyrTyr: 0.63 ± 0.028	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3711 proteins (1149817 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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