Streptococcus iniae (Streptococcus shiloi)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae; Streptococcus

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2124 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1J0MZT5|A0A1J0MZT5_STRIN PTS sugar transporter subunit IIB OS=Streptococcus iniae OX=1346 GN=DIY07_06760 PE=4 SV=1
MM1 pKa = 7.62ALAKK5 pKa = 9.78IVYY8 pKa = 10.47ASMTGNTEE16 pKa = 3.89EE17 pKa = 4.77IADD20 pKa = 3.55IVANKK25 pKa = 9.75LQEE28 pKa = 4.46LGHH31 pKa = 6.43DD32 pKa = 3.95VEE34 pKa = 5.37IDD36 pKa = 3.09EE37 pKa = 4.88CTSVDD42 pKa = 3.54ASDD45 pKa = 5.99FEE47 pKa = 4.61DD48 pKa = 4.92ADD50 pKa = 3.77MAIVATYY57 pKa = 9.3TYY59 pKa = 11.24GDD61 pKa = 3.61GDD63 pKa = 4.31LPDD66 pKa = 5.1EE67 pKa = 4.45IVDD70 pKa = 4.62FYY72 pKa = 11.83EE73 pKa = 5.1DD74 pKa = 4.37LQDD77 pKa = 4.53LDD79 pKa = 4.63LSGKK83 pKa = 9.3VFGVVGSGDD92 pKa = 3.08TFYY95 pKa = 11.14DD96 pKa = 3.99YY97 pKa = 11.04FCKK100 pKa = 10.6SVDD103 pKa = 3.33EE104 pKa = 4.75FEE106 pKa = 4.42EE107 pKa = 4.54QFVLTGASKK116 pKa = 10.53GAASVKK122 pKa = 10.1VDD124 pKa = 4.46LAAEE128 pKa = 4.46DD129 pKa = 3.99EE130 pKa = 4.97DD131 pKa = 4.5IEE133 pKa = 4.32HH134 pKa = 7.24LEE136 pKa = 4.17TFATEE141 pKa = 3.72MSDD144 pKa = 4.09AINRR148 pKa = 11.84LSS150 pKa = 3.12

Molecular weight: 16.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1J0N197|A0A1J0N197_STRIN L-ribulose-5-phosphate 3-epimerase OS=Streptococcus iniae OX=1346 GN=DIY07_09815 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.07QPSKK9 pKa = 9.67IRR11 pKa = 11.84RR12 pKa = 11.84QRR14 pKa = 11.84KK15 pKa = 7.52HH16 pKa = 5.18GFRR19 pKa = 11.84HH20 pKa = 6.46RR21 pKa = 11.84MATKK25 pKa = 9.46NGRR28 pKa = 11.84RR29 pKa = 11.84VLASRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.73GRR39 pKa = 11.84KK40 pKa = 8.76VLSAA44 pKa = 4.05

Molecular weight: 5.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2124

0

2124

665596

21

2879

313.4

35.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.428 ± 0.068

0.601 ± 0.016

5.691 ± 0.043

6.576 ± 0.06

4.615 ± 0.045

6.231 ± 0.051

2.002 ± 0.024

7.502 ± 0.052

7.279 ± 0.053

10.227 ± 0.072

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.593 ± 0.03

4.647 ± 0.038

3.244 ± 0.031

4.195 ± 0.037

3.669 ± 0.036

6.638 ± 0.078

5.694 ± 0.053

6.55 ± 0.048

0.844 ± 0.016

3.774 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski