Emcibacter nanhaiensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Emcibacterales; Emcibacteraceae; Emcibacter

Average proteome isoelectric point is 5.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3495 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A501PFG6|A0A501PFG6_9PROT Uncharacterized protein OS=Emcibacter nanhaiensis OX=1505037 GN=FIV46_13990 PE=4 SV=1
MM1 pKa = 7.55KK2 pKa = 10.64KK3 pKa = 9.64MISSLILSAGLAFAASSAFAAAIYY27 pKa = 9.55TDD29 pKa = 3.52QASFEE34 pKa = 4.18ATLTDD39 pKa = 3.87IVMDD43 pKa = 5.5DD44 pKa = 4.19FNDD47 pKa = 3.65DD48 pKa = 3.25NAEE51 pKa = 3.88YY52 pKa = 11.18DD53 pKa = 4.14FVNSAANMKK62 pKa = 9.77ALSSGSIGYY71 pKa = 9.78EE72 pKa = 3.8STGWSAPDD80 pKa = 3.34WNIVGSGYY88 pKa = 9.13MCWGCNGSGMILLDD102 pKa = 3.79DD103 pKa = 4.3TNITAANGVFGVGFDD118 pKa = 3.57IRR120 pKa = 11.84WNGGSLPYY128 pKa = 10.37DD129 pKa = 3.38AFVTYY134 pKa = 10.98GDD136 pKa = 4.22GSTEE140 pKa = 4.05TFDD143 pKa = 4.07LGSGARR149 pKa = 11.84FFGLTSLDD157 pKa = 4.53LISSVHH163 pKa = 5.02FTQEE167 pKa = 3.87SGEE170 pKa = 4.04PTIDD174 pKa = 3.36GSFGLYY180 pKa = 10.17SVTIGNAAEE189 pKa = 4.28VPEE192 pKa = 4.37PAPLALLGLGLVGFGLARR210 pKa = 11.84CKK212 pKa = 10.47RR213 pKa = 3.68

Molecular weight: 22.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A501PEP6|A0A501PEP6_9PROT 2-hydroxychromene-2-carboxylate isomerase OS=Emcibacter nanhaiensis OX=1505037 GN=FIV46_14015 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLVRR12 pKa = 11.84KK13 pKa = 9.31RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84SASVGGRR28 pKa = 11.84RR29 pKa = 11.84VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.92GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3495

0

3495

1175881

33

3610

336.4

37.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.471 ± 0.049

0.913 ± 0.014

6.26 ± 0.042

6.919 ± 0.039

3.956 ± 0.029

8.11 ± 0.052

2.059 ± 0.022

5.774 ± 0.032

4.596 ± 0.038

10.013 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.709 ± 0.022

3.453 ± 0.027

4.468 ± 0.027

3.332 ± 0.025

5.723 ± 0.044

5.898 ± 0.037

5.209 ± 0.045

7.001 ± 0.038

1.229 ± 0.017

2.907 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski