Amino acid dipepetide frequency for Emcibacter nanhaiensis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	10.532AlaAla: 10.532 ± 0.142	0.96AlaCys: 0.96 ± 0.031	5.823AlaAsp: 5.823 ± 0.085	7.089AlaGlu: 7.089 ± 0.103	3.56AlaPhe: 3.56 ± 0.055	8.605AlaGly: 8.605 ± 0.115	1.788AlaHis: 1.788 ± 0.05	5.119AlaIle: 5.119 ± 0.056	3.771AlaLys: 3.771 ± 0.06	9.816AlaLeu: 9.816 ± 0.113	2.637AlaMet: 2.637 ± 0.049	2.736AlaAsn: 2.736 ± 0.051	3.71AlaPro: 3.71 ± 0.065	2.877AlaGln: 2.877 ± 0.05	5.827AlaArg: 5.827 ± 0.083	5.156AlaSer: 5.156 ± 0.076	4.532AlaThr: 4.532 ± 0.076	6.653AlaVal: 6.653 ± 0.089	1.04AlaTrp: 1.04 ± 0.031	2.482AlaTyr: 2.482 ± 0.045	0.0AlaXaa: 0.0 ± 0.0
Cys	0.827CysAla: 0.827 ± 0.027	0.144CysCys: 0.144 ± 0.011	0.597CysAsp: 0.597 ± 0.024	0.475CysGlu: 0.475 ± 0.024	0.382CysPhe: 0.382 ± 0.017	0.902CysGly: 0.902 ± 0.027	0.277CysHis: 0.277 ± 0.017	0.445CysIle: 0.445 ± 0.021	0.289CysLys: 0.289 ± 0.015	0.956CysLeu: 0.956 ± 0.034	0.199CysMet: 0.199 ± 0.013	0.281CysAsn: 0.281 ± 0.015	0.525CysPro: 0.525 ± 0.023	0.275CysGln: 0.275 ± 0.014	0.618CysArg: 0.618 ± 0.024	0.534CysSer: 0.534 ± 0.021	0.453CysThr: 0.453 ± 0.022	0.575CysVal: 0.575 ± 0.026	0.112CysTrp: 0.112 ± 0.011	0.267CysTyr: 0.267 ± 0.017	0.0CysXaa: 0.0 ± 0.0
Asp	4.928AspAla: 4.928 ± 0.074	0.534AspCys: 0.534 ± 0.021	3.573AspAsp: 3.573 ± 0.074	4.225AspGlu: 4.225 ± 0.063	2.691AspPhe: 2.691 ± 0.052	5.368AspGly: 5.368 ± 0.104	1.431AspHis: 1.431 ± 0.035	4.092AspIle: 4.092 ± 0.066	3.016AspLys: 3.016 ± 0.057	6.373AspLeu: 6.373 ± 0.078	1.867AspMet: 1.867 ± 0.044	2.217AspAsn: 2.217 ± 0.056	2.992AspPro: 2.992 ± 0.049	2.136AspGln: 2.136 ± 0.047	3.861AspArg: 3.861 ± 0.059	2.983AspSer: 2.983 ± 0.05	2.583AspThr: 2.583 ± 0.06	4.368AspVal: 4.368 ± 0.071	1.066AspTrp: 1.066 ± 0.029	2.3AspTyr: 2.3 ± 0.043	0.0AspXaa: 0.0 ± 0.0
Glu	6.843GluAla: 6.843 ± 0.09	0.42GluCys: 0.42 ± 0.017	4.27GluAsp: 4.27 ± 0.064	5.792GluGlu: 5.792 ± 0.103	2.426GluPhe: 2.426 ± 0.049	4.878GluGly: 4.878 ± 0.077	1.385GluHis: 1.385 ± 0.039	4.381GluIle: 4.381 ± 0.066	4.427GluLys: 4.427 ± 0.066	6.507GluLeu: 6.507 ± 0.081	2.013GluMet: 2.013 ± 0.04	2.919GluAsn: 2.919 ± 0.051	2.244GluPro: 2.244 ± 0.049	3.083GluGln: 3.083 ± 0.055	3.86GluArg: 3.86 ± 0.068	2.936GluSer: 2.936 ± 0.052	3.68GluThr: 3.68 ± 0.06	4.776GluVal: 4.776 ± 0.071	0.724GluTrp: 0.724 ± 0.025	1.62GluTyr: 1.62 ± 0.038	0.0GluXaa: 0.0 ± 0.0
Phe	3.509PheAla: 3.509 ± 0.059	0.466PheCys: 0.466 ± 0.022	2.826PheAsp: 2.826 ± 0.058	2.476PheGlu: 2.476 ± 0.042	1.629PhePhe: 1.629 ± 0.043	3.428PheGly: 3.428 ± 0.058	0.845PheHis: 0.845 ± 0.024	2.127PheIle: 2.127 ± 0.046	1.437PheLys: 1.437 ± 0.041	3.949PheLeu: 3.949 ± 0.074	1.089PheMet: 1.089 ± 0.036	1.435PheAsn: 1.435 ± 0.033	1.608PhePro: 1.608 ± 0.039	1.138PheGln: 1.138 ± 0.036	2.231PheArg: 2.231 ± 0.052	2.81PheSer: 2.81 ± 0.055	2.23PheThr: 2.23 ± 0.046	2.521PheVal: 2.521 ± 0.049	0.524PheTrp: 0.524 ± 0.022	1.283PheTyr: 1.283 ± 0.032	0.0PheXaa: 0.0 ± 0.0
Gly	6.973GlyAla: 6.973 ± 0.103	0.811GlyCys: 0.811 ± 0.025	4.758GlyAsp: 4.758 ± 0.091	5.343GlyGlu: 5.343 ± 0.07	3.567GlyPhe: 3.567 ± 0.064	6.759GlyGly: 6.759 ± 0.122	1.817GlyHis: 1.817 ± 0.038	4.74GlyIle: 4.74 ± 0.064	4.021GlyLys: 4.021 ± 0.059	8.188GlyLeu: 8.188 ± 0.091	2.311GlyMet: 2.311 ± 0.041	2.766GlyAsn: 2.766 ± 0.058	2.924GlyPro: 2.924 ± 0.057	2.853GlyGln: 2.853 ± 0.052	4.793GlyArg: 4.793 ± 0.068	4.522GlySer: 4.522 ± 0.08	4.495GlyThr: 4.495 ± 0.093	5.511GlyVal: 5.511 ± 0.077	1.139GlyTrp: 1.139 ± 0.032	2.806GlyTyr: 2.806 ± 0.056	0.0GlyXaa: 0.0 ± 0.0
His	1.663HisAla: 1.663 ± 0.037	0.247HisCys: 0.247 ± 0.015	1.151HisAsp: 1.151 ± 0.035	1.219HisGlu: 1.219 ± 0.034	0.894HisPhe: 0.894 ± 0.029	1.67HisGly: 1.67 ± 0.038	0.59HisHis: 0.59 ± 0.024	1.277HisIle: 1.277 ± 0.036	0.907HisLys: 0.907 ± 0.028	2.117HisLeu: 2.117 ± 0.047	0.591HisMet: 0.591 ± 0.022	0.718HisAsn: 0.718 ± 0.027	1.233HisPro: 1.233 ± 0.038	0.651HisGln: 0.651 ± 0.021	1.169HisArg: 1.169 ± 0.036	1.124HisSer: 1.124 ± 0.031	0.895HisThr: 0.895 ± 0.029	1.426HisVal: 1.426 ± 0.039	0.314HisTrp: 0.314 ± 0.015	0.734HisTyr: 0.734 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	5.556IleAla: 5.556 ± 0.075	0.627IleCys: 0.627 ± 0.023	3.83IleAsp: 3.83 ± 0.063	3.897IleGlu: 3.897 ± 0.054	2.293IlePhe: 2.293 ± 0.054	4.697IleGly: 4.697 ± 0.078	1.135IleHis: 1.135 ± 0.03	3.162IleIle: 3.162 ± 0.068	2.29IleLys: 2.29 ± 0.049	5.675IleLeu: 5.675 ± 0.088	1.309IleMet: 1.309 ± 0.039	2.141IleAsn: 2.141 ± 0.045	2.664IlePro: 2.664 ± 0.047	1.527IleGln: 1.527 ± 0.034	3.41IleArg: 3.41 ± 0.058	4.081IleSer: 4.081 ± 0.057	3.289IleThr: 3.289 ± 0.063	3.918IleVal: 3.918 ± 0.062	0.616IleTrp: 0.616 ± 0.023	1.63IleTyr: 1.63 ± 0.04	0.0IleXaa: 0.0 ± 0.0
Lys	4.506LysAla: 4.506 ± 0.076	0.277LysCys: 0.277 ± 0.017	2.619LysAsp: 2.619 ± 0.056	3.028LysGlu: 3.028 ± 0.061	1.61LysPhe: 1.61 ± 0.043	3.369LysGly: 3.369 ± 0.058	0.855LysHis: 0.855 ± 0.03	2.869LysIle: 2.869 ± 0.053	2.817LysLys: 2.817 ± 0.07	4.512LysLeu: 4.512 ± 0.073	1.401LysMet: 1.401 ± 0.036	1.77LysAsn: 1.77 ± 0.04	2.021LysPro: 2.021 ± 0.042	1.612LysGln: 1.612 ± 0.041	2.404LysArg: 2.404 ± 0.048	2.753LysSer: 2.753 ± 0.048	2.448LysThr: 2.448 ± 0.053	3.219LysVal: 3.219 ± 0.055	0.52LysTrp: 0.52 ± 0.024	1.352LysTyr: 1.352 ± 0.039	0.0LysXaa: 0.0 ± 0.0
Leu	10.237LeuAla: 10.237 ± 0.116	0.964LeuCys: 0.964 ± 0.032	6.467LeuAsp: 6.467 ± 0.075	6.971LeuGlu: 6.971 ± 0.084	3.812LeuPhe: 3.812 ± 0.068	7.507LeuGly: 7.507 ± 0.087	1.869LeuHis: 1.869 ± 0.045	5.171LeuIle: 5.171 ± 0.084	4.904LeuLys: 4.904 ± 0.068	9.43LeuLeu: 9.43 ± 0.136	2.648LeuMet: 2.648 ± 0.048	3.41LeuAsn: 3.41 ± 0.051	4.981LeuPro: 4.981 ± 0.074	3.164LeuGln: 3.164 ± 0.052	5.288LeuArg: 5.288 ± 0.069	7.112LeuSer: 7.112 ± 0.085	5.56LeuThr: 5.56 ± 0.082	6.974LeuVal: 6.974 ± 0.092	1.039LeuTrp: 1.039 ± 0.031	2.617LeuTyr: 2.617 ± 0.047	0.0LeuXaa: 0.0 ± 0.0
Met	2.817MetAla: 2.817 ± 0.045	0.183MetCys: 0.183 ± 0.013	1.653MetAsp: 1.653 ± 0.041	1.771MetGlu: 1.771 ± 0.044	0.859MetPhe: 0.859 ± 0.031	2.024MetGly: 2.024 ± 0.045	0.471MetHis: 0.471 ± 0.021	1.636MetIle: 1.636 ± 0.039	1.494MetLys: 1.494 ± 0.034	2.391MetLeu: 2.391 ± 0.049	0.765MetMet: 0.765 ± 0.032	0.956MetAsn: 0.956 ± 0.026	1.371MetPro: 1.371 ± 0.037	0.862MetGln: 0.862 ± 0.027	1.337MetArg: 1.337 ± 0.036	1.925MetSer: 1.925 ± 0.038	2.021MetThr: 2.021 ± 0.036	1.828MetVal: 1.828 ± 0.043	0.245MetTrp: 0.245 ± 0.016	0.487MetTyr: 0.487 ± 0.024	0.0MetXaa: 0.0 ± 0.0
Asn	3.106AsnAla: 3.106 ± 0.063	0.333AsnCys: 0.333 ± 0.018	1.957AsnAsp: 1.957 ± 0.046	1.8AsnGlu: 1.8 ± 0.043	1.419AsnPhe: 1.419 ± 0.038	2.732AsnGly: 2.732 ± 0.051	0.646AsnHis: 0.646 ± 0.023	2.529AsnIle: 2.529 ± 0.055	1.439AsnLys: 1.439 ± 0.039	3.48AsnLeu: 3.48 ± 0.06	1.057AsnMet: 1.057 ± 0.031	1.293AsnAsn: 1.293 ± 0.035	1.97AsnPro: 1.97 ± 0.036	1.011AsnGln: 1.011 ± 0.032	2.058AsnArg: 2.058 ± 0.037	2.017AsnSer: 2.017 ± 0.053	1.601AsnThr: 1.601 ± 0.044	2.391AsnVal: 2.391 ± 0.053	0.568AsnTrp: 0.568 ± 0.023	1.126AsnTyr: 1.126 ± 0.03	0.0AsnXaa: 0.0 ± 0.0
Pro	4.406ProAla: 4.406 ± 0.066	0.371ProCys: 0.371 ± 0.019	3.483ProAsp: 3.483 ± 0.054	4.269ProGlu: 4.269 ± 0.067	1.787ProPhe: 1.787 ± 0.044	3.881ProGly: 3.881 ± 0.057	0.908ProHis: 0.908 ± 0.03	1.999ProIle: 1.999 ± 0.044	1.713ProLys: 1.713 ± 0.045	4.45ProLeu: 4.45 ± 0.066	1.017ProMet: 1.017 ± 0.027	1.229ProAsn: 1.229 ± 0.036	1.71ProPro: 1.71 ± 0.049	1.411ProGln: 1.411 ± 0.034	2.03ProArg: 2.03 ± 0.043	2.301ProSer: 2.301 ± 0.044	2.058ProThr: 2.058 ± 0.048	3.82ProVal: 3.82 ± 0.063	0.549ProTrp: 0.549 ± 0.025	1.283ProTyr: 1.283 ± 0.035	0.0ProXaa: 0.0 ± 0.0
Gln	3.552GlnAla: 3.552 ± 0.057	0.225GlnCys: 0.225 ± 0.015	2.016GlnAsp: 2.016 ± 0.033	2.361GlnGlu: 2.361 ± 0.044	1.212GlnPhe: 1.212 ± 0.041	2.431GlnGly: 2.431 ± 0.045	0.634GlnHis: 0.634 ± 0.022	2.026GlnIle: 2.026 ± 0.044	1.874GlnLys: 1.874 ± 0.046	3.09GlnLeu: 3.09 ± 0.054	1.02GlnMet: 1.02 ± 0.031	1.221GlnAsn: 1.221 ± 0.036	1.361GlnPro: 1.361 ± 0.033	1.389GlnGln: 1.389 ± 0.039	1.723GlnArg: 1.723 ± 0.04	1.828GlnSer: 1.828 ± 0.042	1.704GlnThr: 1.704 ± 0.039	2.465GlnVal: 2.465 ± 0.045	0.365GlnTrp: 0.365 ± 0.019	0.823GlnTyr: 0.823 ± 0.026	0.0GlnXaa: 0.0 ± 0.0
Arg	4.893ArgAla: 4.893 ± 0.073	0.423ArgCys: 0.423 ± 0.018	3.699ArgAsp: 3.699 ± 0.064	4.461ArgGlu: 4.461 ± 0.067	2.323ArgPhe: 2.323 ± 0.047	3.676ArgGly: 3.676 ± 0.064	1.441ArgHis: 1.441 ± 0.035	3.45ArgIle: 3.45 ± 0.061	3.011ArgLys: 3.011 ± 0.058	6.488ArgLeu: 6.488 ± 0.086	1.438ArgMet: 1.438 ± 0.033	2.022ArgAsn: 2.022 ± 0.044	2.405ArgPro: 2.405 ± 0.052	2.391ArgGln: 2.391 ± 0.054	3.643ArgArg: 3.643 ± 0.067	2.8ArgSer: 2.8 ± 0.046	2.715ArgThr: 2.715 ± 0.053	3.603ArgVal: 3.603 ± 0.059	0.645ArgTrp: 0.645 ± 0.027	1.703ArgTyr: 1.703 ± 0.043	0.0ArgXaa: 0.0 ± 0.0
Ser	5.243SerAla: 5.243 ± 0.075	0.569SerCys: 0.569 ± 0.022	3.592SerAsp: 3.592 ± 0.067	3.512SerGlu: 3.512 ± 0.052	2.665SerPhe: 2.665 ± 0.051	5.682SerGly: 5.682 ± 0.103	1.242SerHis: 1.242 ± 0.033	3.147SerIle: 3.147 ± 0.053	2.115SerLys: 2.115 ± 0.043	6.416SerLeu: 6.416 ± 0.081	1.498SerMet: 1.498 ± 0.038	1.834SerAsn: 1.834 ± 0.044	2.806SerPro: 2.806 ± 0.047	1.895SerGln: 1.895 ± 0.043	3.573SerArg: 3.573 ± 0.062	3.685SerSer: 3.685 ± 0.074	2.664SerThr: 2.664 ± 0.053	4.099SerVal: 4.099 ± 0.067	0.81SerTrp: 0.81 ± 0.027	1.934SerTyr: 1.934 ± 0.054	0.0SerXaa: 0.0 ± 0.0
Thr	5.074ThrAla: 5.074 ± 0.081	0.456ThrCys: 0.456 ± 0.021	3.241ThrAsp: 3.241 ± 0.073	3.244ThrGlu: 3.244 ± 0.045	2.033ThrPhe: 2.033 ± 0.04	5.03ThrGly: 5.03 ± 0.082	0.958ThrHis: 0.958 ± 0.029	3.055ThrIle: 3.055 ± 0.057	1.745ThrLys: 1.745 ± 0.043	5.415ThrLeu: 5.415 ± 0.073	1.166ThrMet: 1.166 ± 0.028	1.504ThrAsn: 1.504 ± 0.039	2.98ThrPro: 2.98 ± 0.053	1.356ThrGln: 1.356 ± 0.036	2.692ThrArg: 2.692 ± 0.054	3.213ThrSer: 3.213 ± 0.057	2.672ThrThr: 2.672 ± 0.067	4.147ThrVal: 4.147 ± 0.076	0.629ThrTrp: 0.629 ± 0.036	1.475ThrTyr: 1.475 ± 0.039	0.0ThrXaa: 0.0 ± 0.0
Val	6.704ValAla: 6.704 ± 0.082	0.691ValCys: 0.691 ± 0.025	4.309ValAsp: 4.309 ± 0.065	4.836ValGlu: 4.836 ± 0.076	2.561ValPhe: 2.561 ± 0.046	5.026ValGly: 5.026 ± 0.067	1.312ValHis: 1.312 ± 0.032	4.315ValIle: 4.315 ± 0.052	3.031ValLys: 3.031 ± 0.057	6.519ValLeu: 6.519 ± 0.09	1.989ValMet: 1.989 ± 0.044	2.601ValAsn: 2.601 ± 0.049	3.372ValPro: 3.372 ± 0.054	1.99ValGln: 1.99 ± 0.039	4.011ValArg: 4.011 ± 0.058	4.666ValSer: 4.666 ± 0.079	4.376ValThr: 4.376 ± 0.082	5.094ValVal: 5.094 ± 0.079	0.81ValTrp: 0.81 ± 0.028	1.792ValTyr: 1.792 ± 0.044	0.0ValXaa: 0.0 ± 0.0
Trp	0.929TrpAla: 0.929 ± 0.029	0.128TrpCys: 0.128 ± 0.011	0.724TrpAsp: 0.724 ± 0.026	0.654TrpGlu: 0.654 ± 0.029	0.506TrpPhe: 0.506 ± 0.02	0.884TrpGly: 0.884 ± 0.028	0.295TrpHis: 0.295 ± 0.017	0.662TrpIle: 0.662 ± 0.022	0.555TrpLys: 0.555 ± 0.021	1.367TrpLeu: 1.367 ± 0.042	0.368TrpMet: 0.368 ± 0.019	0.504TrpAsn: 0.504 ± 0.02	0.572TrpPro: 0.572 ± 0.021	0.658TrpGln: 0.658 ± 0.025	0.795TrpArg: 0.795 ± 0.026	0.779TrpSer: 0.779 ± 0.022	0.668TrpThr: 0.668 ± 0.028	0.721TrpVal: 0.721 ± 0.025	0.183TrpTrp: 0.183 ± 0.012	0.334TrpTyr: 0.334 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.415TyrAla: 2.415 ± 0.043	0.306TyrCys: 0.306 ± 0.015	2.013TyrAsp: 2.013 ± 0.048	1.762TyrGlu: 1.762 ± 0.041	1.33TyrPhe: 1.33 ± 0.038	2.547TyrGly: 2.547 ± 0.056	0.69TyrHis: 0.69 ± 0.022	1.542TyrIle: 1.542 ± 0.032	1.104TyrLys: 1.104 ± 0.028	2.991TyrLeu: 2.991 ± 0.049	0.711TyrMet: 0.711 ± 0.024	0.975TyrAsn: 0.975 ± 0.029	1.228TyrPro: 1.228 ± 0.03	1.041TyrGln: 1.041 ± 0.028	1.906TyrArg: 1.906 ± 0.04	1.754TyrSer: 1.754 ± 0.041	1.443TyrThr: 1.443 ± 0.042	1.899TyrVal: 1.899 ± 0.043	0.39TyrTrp: 0.39 ± 0.017	1.021TyrTyr: 1.021 ± 0.032	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3495 proteins (1175881 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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