Falconid herpesvirus 1
Average proteome isoelectric point is 7.15
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 108 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A068EVV2|A0A068EVV2_9ALPH OriBP OS=Falconid herpesvirus 1 OX=1510155 GN=FaHV1S18_019 PE=3 SV=1
MM1 pKa = 7.49 SSAIATFITYY11 pKa = 8.14 TFVGAKK17 pKa = 8.9 TSPTWTVPNYY27 pKa = 9.34 EE28 pKa = 4.67 QIICSCDD35 pKa = 2.68 GGTRR39 pKa = 11.84 SVAVGGMSRR48 pKa = 11.84 CDD50 pKa = 3.54 YY51 pKa = 11.18 LPATNVILQRR61 pKa = 11.84 GPAGTLLILDD71 pKa = 4.67 GGVEE75 pKa = 4.34 FCSYY79 pKa = 11.2 LLRR82 pKa = 11.84 SEE84 pKa = 4.9 DD85 pKa = 3.43 ATSARR90 pKa = 11.84 SSGYY94 pKa = 10.26 LSSAPGSICVVPFSSCTVIGLDD116 pKa = 3.77 TYY118 pKa = 11.38 VCNNSSGVLTVTWLRR133 pKa = 11.84 QAAYY137 pKa = 8.21 ITLTVYY143 pKa = 10.89 GSGHH147 pKa = 6.49 EE148 pKa = 4.13 YY149 pKa = 10.42 RR150 pKa = 11.84 ISEE153 pKa = 4.56 PGSVSQSSCSSVASQKK169 pKa = 11.14 DD170 pKa = 3.49 SAAEE174 pKa = 4.04 SCEE177 pKa = 3.62 GRR179 pKa = 11.84 AVPTPEE185 pKa = 4.33 NRR187 pKa = 11.84 VPDD190 pKa = 4.24 DD191 pKa = 3.59 AVAEE195 pKa = 4.23 ARR197 pKa = 11.84 DD198 pKa = 3.84 GASAPRR204 pKa = 11.84 EE205 pKa = 3.97 PDD207 pKa = 3.31 DD208 pKa = 4.03 VLDD211 pKa = 6.77 EE212 pKa = 4.67 IIQWNVHH219 pKa = 5.15 GPGAGLAGALSPGSTPTQTKK239 pKa = 9.81 NGEE242 pKa = 4.15 GGGDD246 pKa = 3.56 LLAAAMRR253 pKa = 11.84 EE254 pKa = 4.03 SHH256 pKa = 6.9 LGVEE260 pKa = 4.63 PLSFSDD266 pKa = 4.89 IDD268 pKa = 3.52 VDD270 pKa = 5.09 LPNIPIPLPAQSPPAIAPVEE290 pKa = 4.3 YY291 pKa = 9.98 IFQDD295 pKa = 3.07 NDD297 pKa = 2.87 QPADD301 pKa = 3.86 FGFLSSGPP309 pKa = 3.69
Molecular weight: 32.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.97
IPC2_protein 4.19
IPC_protein 4.164
Toseland 3.961
ProMoST 4.304
Dawson 4.139
Bjellqvist 4.279
Wikipedia 4.037
Rodwell 3.986
Grimsley 3.872
Solomon 4.126
Lehninger 4.088
Nozaki 4.24
DTASelect 4.457
Thurlkill 3.999
EMBOSS 4.05
Sillero 4.279
Patrickios 0.795
IPC_peptide 4.126
IPC2_peptide 4.266
IPC2.peptide.svr19 4.151
Protein with the highest isoelectric point:
>tr|A0A068ER91|A0A068ER91_9ALPH Serine/threonine protein kinase US3 OS=Falconid herpesvirus 1 OX=1510155 GN=FaHV1S18_107 PE=4 SV=1
MM1 pKa = 7.64 GLPLRR6 pKa = 11.84 RR7 pKa = 11.84 SSRR10 pKa = 11.84 IRR12 pKa = 11.84 GAAGHH17 pKa = 6.12 VATALVSRR25 pKa = 11.84 LEE27 pKa = 3.92 TAARR31 pKa = 11.84 YY32 pKa = 9.48 ASGGRR37 pKa = 11.84 RR38 pKa = 11.84 PVTRR42 pKa = 11.84 SCAVGRR48 pKa = 11.84 LHH50 pKa = 7.31 LGVGINNRR58 pKa = 11.84 RR59 pKa = 11.84 VRR61 pKa = 11.84 GGPQTSPSAASQPASAIPASRR82 pKa = 11.84 RR83 pKa = 11.84 TALGMRR89 pKa = 11.84 GVGGVIGEE97 pKa = 4.19 VAKK100 pKa = 10.54 RR101 pKa = 11.84 VAGGEE106 pKa = 4.12 RR107 pKa = 11.84 AASAPRR113 pKa = 11.84 ARR115 pKa = 11.84 RR116 pKa = 11.84 RR117 pKa = 11.84 PRR119 pKa = 11.84 SSLNSNAAAAAAAAAATGSDD139 pKa = 4.05 APQMVPGQTKK149 pKa = 9.94 KK150 pKa = 10.73 CGAPKK155 pKa = 10.35 RR156 pKa = 11.84 STAATTTTTATTADD170 pKa = 4.38 GGCSCAGGGAACGAHH185 pKa = 5.78 HH186 pKa = 7.35 RR187 pKa = 11.84 EE188 pKa = 3.98 RR189 pKa = 11.84 DD190 pKa = 3.12 GHH192 pKa = 5.11 RR193 pKa = 11.84 RR194 pKa = 11.84 RR195 pKa = 11.84 TTVCMGTCAIGRR207 pKa = 11.84 RR208 pKa = 11.84 RR209 pKa = 11.84 SDD211 pKa = 2.8 ASRR214 pKa = 11.84 EE215 pKa = 4.31 CACMPPPASTTPAGAGAVAAAAVAPCVDD243 pKa = 3.46 SRR245 pKa = 11.84 TLRR248 pKa = 11.84 PQQRR252 pKa = 11.84 PHH254 pKa = 5.73 PRR256 pKa = 11.84 SRR258 pKa = 11.84 RR259 pKa = 11.84 GTVSPIEE266 pKa = 4.16 ASVASPAGPSDD277 pKa = 3.29 ADD279 pKa = 3.17 YY280 pKa = 11.32 RR281 pKa = 11.84 RR282 pKa = 11.84 LYY284 pKa = 10.29 KK285 pKa = 10.52 CRR287 pKa = 11.84 LDD289 pKa = 4.07 TEE291 pKa = 4.18 EE292 pKa = 4.87 HH293 pKa = 6.18 AASLPRR299 pKa = 11.84 SVKK302 pKa = 10.16 DD303 pKa = 3.34 LAHH306 pKa = 6.26 ITHH309 pKa = 6.81 TAARR313 pKa = 11.84 DD314 pKa = 3.33 AYY316 pKa = 9.04 SAMRR320 pKa = 11.84 AGMPPPARR328 pKa = 11.84 LWKK331 pKa = 10.32 IVGCKK336 pKa = 9.98 FKK338 pKa = 11.12 ALLQSYY344 pKa = 8.19 EE345 pKa = 4.0 RR346 pKa = 11.84 SAPPFNPSEE355 pKa = 4.63 PIRR358 pKa = 11.84 TVAAKK363 pKa = 10.35 AFRR366 pKa = 11.84 AIAAVPPASHH376 pKa = 6.5 QEE378 pKa = 3.86 LSDD381 pKa = 3.52 ALTLVMYY388 pKa = 7.4 WCCLGHH394 pKa = 6.77 VSRR397 pKa = 11.84 CKK399 pKa = 9.78 MAGLYY404 pKa = 9.38 EE405 pKa = 4.32 RR406 pKa = 11.84 EE407 pKa = 4.09 PAVPALFDD415 pKa = 3.73 ARR417 pKa = 11.84 PGCGEE422 pKa = 4.26 IPPSDD427 pKa = 3.51 VNYY430 pKa = 9.64 WKK432 pKa = 10.26 QLYY435 pKa = 9.96 RR436 pKa = 11.84 LADD439 pKa = 3.43 KK440 pKa = 10.65 GGSSIVPDD448 pKa = 4.06 ASLAAYY454 pKa = 8.75 VALVRR459 pKa = 11.84 RR460 pKa = 11.84 DD461 pKa = 3.49 PDD463 pKa = 3.47 EE464 pKa = 4.26 EE465 pKa = 4.28 LVGG468 pKa = 3.88
Molecular weight: 48.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.352
IPC2_protein 9.545
IPC_protein 10.496
Toseland 10.54
ProMoST 10.35
Dawson 10.672
Bjellqvist 10.452
Wikipedia 10.921
Rodwell 10.73
Grimsley 10.73
Solomon 10.804
Lehninger 10.76
Nozaki 10.599
DTASelect 10.423
Thurlkill 10.57
EMBOSS 10.965
Sillero 10.613
Patrickios 10.379
IPC_peptide 10.804
IPC2_peptide 9.867
IPC2.peptide.svr19 8.527
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
108
0
108
47844
77
3279
443.0
48.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.064 ± 0.33
2.061 ± 0.132
6.235 ± 0.202
5.445 ± 0.151
3.415 ± 0.205
7.725 ± 0.271
1.906 ± 0.076
3.936 ± 0.143
3.334 ± 0.185
8.517 ± 0.201
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.285 ± 0.098
2.803 ± 0.141
6.203 ± 0.28
2.573 ± 0.104
8.668 ± 0.28
8.214 ± 0.266
5.788 ± 0.141
6.732 ± 0.169
1.162 ± 0.069
2.932 ± 0.128
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here