Enterococcus aquimarinus
Average proteome isoelectric point is 6.01
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2342 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L8QPS9|A0A1L8QPS9_9ENTE DNA mismatch repair protein MutS OS=Enterococcus aquimarinus OX=328396 GN=mutS PE=3 SV=1
MM1 pKa = 7.73 YY2 pKa = 10.71 YY3 pKa = 9.86 PINYY7 pKa = 9.48 GYY9 pKa = 9.94 IPGIIAPDD17 pKa = 3.88 GDD19 pKa = 3.84 EE20 pKa = 3.68 QDD22 pKa = 3.65 AYY24 pKa = 10.12 IVGVDD29 pKa = 3.65 KK30 pKa = 10.96 PLTTFTGIVIAIITRR45 pKa = 11.84 FDD47 pKa = 3.59 DD48 pKa = 4.85 VEE50 pKa = 4.47 EE51 pKa = 4.24 KK52 pKa = 10.38 WVVAPEE58 pKa = 4.04 NCSFTRR64 pKa = 11.84 EE65 pKa = 4.1 MIRR68 pKa = 11.84 EE69 pKa = 3.67 QVYY72 pKa = 8.94 FQEE75 pKa = 4.97 QYY77 pKa = 11.38 FDD79 pKa = 3.69 TQIQMANDD87 pKa = 4.42 DD88 pKa = 3.7 IKK90 pKa = 11.53
Molecular weight: 10.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.763
IPC2_protein 3.948
IPC_protein 3.872
Toseland 3.668
ProMoST 4.037
Dawson 3.859
Bjellqvist 4.024
Wikipedia 3.795
Rodwell 3.706
Grimsley 3.592
Solomon 3.846
Lehninger 3.795
Nozaki 3.986
DTASelect 4.19
Thurlkill 3.732
EMBOSS 3.808
Sillero 3.999
Patrickios 0.846
IPC_peptide 3.846
IPC2_peptide 3.973
IPC2.peptide.svr19 3.884
Protein with the highest isoelectric point:
>tr|A0A1L8QTW1|A0A1L8QTW1_9ENTE Sporulation initiation inhibitor Soj OS=Enterococcus aquimarinus OX=328396 GN=RU93_GL001796 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.14 QPSKK9 pKa = 9.1 RR10 pKa = 11.84 KK11 pKa = 9.07 HH12 pKa = 5.9 SKK14 pKa = 8.56 VHH16 pKa = 5.77 GFRR19 pKa = 11.84 KK20 pKa = 10.02 RR21 pKa = 11.84 MSTKK25 pKa = 9.54 NGRR28 pKa = 11.84 RR29 pKa = 11.84 VIASRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.71 GRR39 pKa = 11.84 KK40 pKa = 8.76 VLSAA44 pKa = 4.05
Molecular weight: 5.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.415
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.398
Grimsley 12.661
Solomon 13.115
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.135
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.013
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2342
0
2342
724622
37
1458
309.4
34.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.306 ± 0.06
0.622 ± 0.013
5.06 ± 0.041
7.578 ± 0.055
4.695 ± 0.042
6.579 ± 0.042
1.969 ± 0.024
7.68 ± 0.052
6.182 ± 0.04
10.007 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.719 ± 0.023
4.366 ± 0.033
3.573 ± 0.025
4.569 ± 0.039
3.927 ± 0.031
5.749 ± 0.036
5.93 ± 0.036
6.821 ± 0.044
0.957 ± 0.02
3.712 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here