Algoriella xinjiangensis
Average proteome isoelectric point is 6.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3034 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1I4WNT3|A0A1I4WNT3_9FLAO Uncharacterized protein OS=Algoriella xinjiangensis OX=684065 GN=SAMN05421738_10784 PE=4 SV=1
MM1 pKa = 8.23 DD2 pKa = 5.37 FLDD5 pKa = 5.24 DD6 pKa = 4.63 LLNSSTLPTEE16 pKa = 5.56 RR17 pKa = 11.84 ISQMLDD23 pKa = 2.77 EE24 pKa = 5.06 DD25 pKa = 4.9 LFWQIIEE32 pKa = 4.43 NSLIDD37 pKa = 4.43 ADD39 pKa = 4.09 NLHH42 pKa = 6.76 DD43 pKa = 3.83 QEE45 pKa = 5.7 KK46 pKa = 9.74 YY47 pKa = 10.4 LSKK50 pKa = 10.56 EE51 pKa = 3.91 LEE53 pKa = 4.04 NLVAEE58 pKa = 5.12 DD59 pKa = 3.85 IIGFQLRR66 pKa = 11.84 LEE68 pKa = 4.25 YY69 pKa = 10.8 NLYY72 pKa = 10.64 LLHH75 pKa = 6.87 TPEE78 pKa = 4.53 AWCAACIMNDD88 pKa = 3.52 DD89 pKa = 3.98 TDD91 pKa = 3.72 VHH93 pKa = 7.11 HH94 pKa = 7.19 FFYY97 pKa = 10.55 FKK99 pKa = 10.62 SWVISQGQEE108 pKa = 3.78 MFEE111 pKa = 4.09 KK112 pKa = 10.36 TILHH116 pKa = 7.04 PDD118 pKa = 3.08 NLAEE122 pKa = 4.22 YY123 pKa = 9.96 FDD125 pKa = 5.0 EE126 pKa = 5.73 GFNDD130 pKa = 3.46 DD131 pKa = 5.3 DD132 pKa = 5.11 LYY134 pKa = 11.49 EE135 pKa = 4.19 FEE137 pKa = 4.82 NFNMIADD144 pKa = 3.58 NVFYY148 pKa = 10.76 EE149 pKa = 4.22 KK150 pKa = 10.58 FEE152 pKa = 4.25 HH153 pKa = 7.1 KK154 pKa = 10.37 IIDD157 pKa = 4.64 FIDD160 pKa = 3.44 KK161 pKa = 10.91 NFFQYY166 pKa = 9.22 LTQNSPKK173 pKa = 10.35 PEE175 pKa = 4.09 FDD177 pKa = 3.29 WDD179 pKa = 4.1 DD180 pKa = 3.67 EE181 pKa = 4.4 NLASLQAICPTLFQIFVEE199 pKa = 5.05 DD200 pKa = 3.96 YY201 pKa = 10.4 SPEE204 pKa = 4.44 DD205 pKa = 3.41 EE206 pKa = 6.19 DD207 pKa = 5.73 YY208 pKa = 11.59 DD209 pKa = 5.01 DD210 pKa = 6.18 EE211 pKa = 4.75 EE212 pKa = 6.77 DD213 pKa = 4.48 EE214 pKa = 6.04 DD215 pKa = 5.69 FDD217 pKa = 6.37 DD218 pKa = 4.61 EE219 pKa = 4.55
Molecular weight: 26.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.718
IPC2_protein 3.643
IPC_protein 3.656
Toseland 3.439
ProMoST 3.808
Dawson 3.643
Bjellqvist 3.821
Wikipedia 3.579
Rodwell 3.49
Grimsley 3.35
Solomon 3.643
Lehninger 3.592
Nozaki 3.757
DTASelect 3.986
Thurlkill 3.49
EMBOSS 3.579
Sillero 3.783
Patrickios 0.922
IPC_peptide 3.643
IPC2_peptide 3.757
IPC2.peptide.svr19 3.723
Protein with the highest isoelectric point:
>tr|A0A1I4T8E4|A0A1I4T8E4_9FLAO Pyrroline-5-carboxylate reductase OS=Algoriella xinjiangensis OX=684065 GN=proC PE=3 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.01 RR4 pKa = 11.84 TFQPSNRR11 pKa = 11.84 KK12 pKa = 8.86 KK13 pKa = 10.03 RR14 pKa = 11.84 NKK16 pKa = 9.59 HH17 pKa = 4.05 GFRR20 pKa = 11.84 EE21 pKa = 4.13 RR22 pKa = 11.84 MASKK26 pKa = 10.54 NGRR29 pKa = 11.84 KK30 pKa = 8.98 ILAARR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.89 KK38 pKa = 10.28 GRR40 pKa = 11.84 AAVSISDD47 pKa = 3.57 VRR49 pKa = 11.84 AKK51 pKa = 10.51 RR52 pKa = 3.37
Molecular weight: 6.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.33
IPC2_protein 10.716
IPC_protein 12.266
Toseland 12.442
ProMoST 12.939
Dawson 12.442
Bjellqvist 12.427
Wikipedia 12.91
Rodwell 12.252
Grimsley 12.486
Solomon 12.939
Lehninger 12.837
Nozaki 12.442
DTASelect 12.427
Thurlkill 12.442
EMBOSS 12.939
Sillero 12.442
Patrickios 11.974
IPC_peptide 12.939
IPC2_peptide 11.915
IPC2.peptide.svr19 9.073
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3034
0
3034
983139
31
2411
324.0
36.77
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.997 ± 0.047
0.676 ± 0.013
5.666 ± 0.032
6.683 ± 0.045
5.428 ± 0.034
5.955 ± 0.04
1.608 ± 0.02
8.484 ± 0.045
8.378 ± 0.043
9.102 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.177 ± 0.021
6.823 ± 0.048
3.144 ± 0.023
3.685 ± 0.028
3.069 ± 0.027
6.179 ± 0.033
5.668 ± 0.034
6.031 ± 0.035
0.956 ± 0.014
4.291 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here