Gordonia phage Eyre

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Eyrevirus; Gordonia virus Eyre

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 74 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B3AZX4|A0A1B3AZX4_9CAUD Tail assembly chaperone OS=Gordonia phage Eyre OX=1887646 GN=14 PE=4 SV=1
MM1 pKa = 7.46ANDD4 pKa = 4.61TIFEE8 pKa = 4.28LPEE11 pKa = 4.65IPGVTFTASYY21 pKa = 10.84GSGGEE26 pKa = 4.12TGLPSNWIRR35 pKa = 11.84IVGTVEE41 pKa = 3.9NPWYY45 pKa = 9.99EE46 pKa = 3.6PAYY49 pKa = 10.96NYY51 pKa = 11.19GLDD54 pKa = 3.61PNGYY58 pKa = 7.8TEE60 pKa = 5.28VTDD63 pKa = 3.16PWKK66 pKa = 10.68RR67 pKa = 11.84HH68 pKa = 3.81TQFPEE73 pKa = 3.86VCAMGFGGPTNTNLPTDD90 pKa = 4.49PPPAAEE96 pKa = 4.22STPEE100 pKa = 3.83EE101 pKa = 4.29LL102 pKa = 5.14

Molecular weight: 11.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B3AZY5|A0A1B3AZY5_9CAUD Holin OS=Gordonia phage Eyre OX=1887646 GN=19 PE=4 SV=1
MM1 pKa = 7.4HH2 pKa = 7.54NDD4 pKa = 2.83IEE6 pKa = 4.44MEE8 pKa = 4.32SKK10 pKa = 10.87VIEE13 pKa = 4.04NWVTHH18 pKa = 6.57LYY20 pKa = 11.14AEE22 pKa = 4.9GKK24 pKa = 9.64SNRR27 pKa = 11.84TVKK30 pKa = 10.42DD31 pKa = 3.07RR32 pKa = 11.84RR33 pKa = 11.84IVLRR37 pKa = 11.84RR38 pKa = 11.84FEE40 pKa = 4.58RR41 pKa = 11.84DD42 pKa = 2.31IGTPVLAANTGQIAAWLGRR61 pKa = 11.84DD62 pKa = 3.35DD63 pKa = 4.62LAPVTRR69 pKa = 11.84SVYY72 pKa = 10.56HH73 pKa = 6.6SILTAFFRR81 pKa = 11.84WAIAVGLRR89 pKa = 11.84GDD91 pKa = 4.17NPVSPIKK98 pKa = 10.11AAKK101 pKa = 9.59RR102 pKa = 11.84PRR104 pKa = 11.84RR105 pKa = 11.84QPRR108 pKa = 11.84PVTPDD113 pKa = 2.83QYY115 pKa = 11.54RR116 pKa = 11.84RR117 pKa = 11.84LLDD120 pKa = 3.88GAAGDD125 pKa = 4.09HH126 pKa = 6.77AMTAMLLLAGLQGLRR141 pKa = 11.84VHH143 pKa = 7.11EE144 pKa = 4.1IARR147 pKa = 11.84FHH149 pKa = 6.7SRR151 pKa = 11.84HH152 pKa = 6.21LDD154 pKa = 3.31VEE156 pKa = 4.18ARR158 pKa = 11.84TLEE161 pKa = 4.09VTGKK165 pKa = 10.6GGASYY170 pKa = 10.55VLPASQPILDD180 pKa = 4.7HH181 pKa = 6.37ARR183 pKa = 11.84HH184 pKa = 5.57MPLTGFWFPSQRR196 pKa = 11.84AKK198 pKa = 10.88HH199 pKa = 5.63LGGRR203 pKa = 11.84TISEE207 pKa = 4.49RR208 pKa = 11.84IHH210 pKa = 7.6LHH212 pKa = 5.48MIRR215 pKa = 11.84HH216 pKa = 5.78RR217 pKa = 11.84VPGTPHH223 pKa = 7.29CLRR226 pKa = 11.84HH227 pKa = 5.95HH228 pKa = 7.24FGTQLVSNGADD239 pKa = 2.95LRR241 pKa = 11.84VAQEE245 pKa = 4.07LLRR248 pKa = 11.84HH249 pKa = 5.97SSLQTTAIYY258 pKa = 10.64VATSDD263 pKa = 3.5DD264 pKa = 3.86RR265 pKa = 11.84KK266 pKa = 10.58RR267 pKa = 11.84EE268 pKa = 4.36AIDD271 pKa = 3.28RR272 pKa = 11.84LAAA275 pKa = 4.24

Molecular weight: 30.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

74

0

74

14615

26

1449

197.5

21.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.138 ± 0.377

0.917 ± 0.159

7.157 ± 0.276

6.179 ± 0.271

2.675 ± 0.121

8.676 ± 0.38

2.073 ± 0.253

4.803 ± 0.183

2.771 ± 0.203

7.52 ± 0.269

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.964 ± 0.097

2.689 ± 0.149

5.932 ± 0.251

3.579 ± 0.288

7.643 ± 0.317

5.323 ± 0.231

6.616 ± 0.277

7.041 ± 0.313

1.977 ± 0.113

2.326 ± 0.131
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski