Amino acid dipepetide frequency for Gordonia phage Eyre

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.97AlaAla: 16.97 ± 1.21
0.616AlaCys: 0.616 ± 0.229
9.854AlaAsp: 9.854 ± 0.89
8.622AlaGlu: 8.622 ± 0.798
3.285AlaPhe: 3.285 ± 0.52
9.101AlaGly: 9.101 ± 0.816
2.19AlaHis: 2.19 ± 0.495
5.885AlaIle: 5.885 ± 0.691
2.669AlaLys: 2.669 ± 0.499
8.896AlaLeu: 8.896 ± 0.892
3.148AlaMet: 3.148 ± 0.422
4.653AlaAsn: 4.653 ± 0.53
6.637AlaPro: 6.637 ± 0.759
5.611AlaGln: 5.611 ± 0.666
8.006AlaArg: 8.006 ± 0.801
4.927AlaSer: 4.927 ± 0.577
7.39AlaThr: 7.39 ± 0.734
8.006AlaVal: 8.006 ± 0.881
1.711AlaTrp: 1.711 ± 0.3
3.148AlaTyr: 3.148 ± 0.397
0.0AlaXaa: 0.0 ± 0.0
Cys
0.753CysAla: 0.753 ± 0.333
0.068CysCys: 0.068 ± 0.083
0.753CysAsp: 0.753 ± 0.219
0.616CysGlu: 0.616 ± 0.252
0.0CysPhe: 0.0 ± 0.0
1.163CysGly: 1.163 ± 0.355
0.479CysHis: 0.479 ± 0.218
0.205CysIle: 0.205 ± 0.128
0.205CysLys: 0.205 ± 0.152
0.411CysLeu: 0.411 ± 0.174
0.068CysMet: 0.068 ± 0.065
0.205CysAsn: 0.205 ± 0.173
0.684CysPro: 0.684 ± 0.278
0.342CysGln: 0.342 ± 0.197
0.89CysArg: 0.89 ± 0.343
0.411CysSer: 0.411 ± 0.185
0.616CysThr: 0.616 ± 0.237
0.89CysVal: 0.89 ± 0.319
0.274CysTrp: 0.274 ± 0.156
0.137CysTyr: 0.137 ± 0.111
0.0CysXaa: 0.0 ± 0.0
Asp
9.101AspAla: 9.101 ± 0.707
0.342AspCys: 0.342 ± 0.192
6.022AspAsp: 6.022 ± 0.844
4.79AspGlu: 4.79 ± 0.415
1.642AspPhe: 1.642 ± 0.331
6.774AspGly: 6.774 ± 0.638
1.848AspHis: 1.848 ± 0.374
2.942AspIle: 2.942 ± 0.499
1.574AspLys: 1.574 ± 0.282
6.98AspLeu: 6.98 ± 0.7
0.89AspMet: 0.89 ± 0.209
1.916AspAsn: 1.916 ± 0.367
5.543AspPro: 5.543 ± 0.72
2.19AspGln: 2.19 ± 0.332
5.269AspArg: 5.269 ± 0.654
2.532AspSer: 2.532 ± 0.419
3.764AspThr: 3.764 ± 0.542
4.927AspVal: 4.927 ± 0.6
1.026AspTrp: 1.026 ± 0.31
1.505AspTyr: 1.505 ± 0.277
0.0AspXaa: 0.0 ± 0.0
Glu
6.295GluAla: 6.295 ± 0.769
0.684GluCys: 0.684 ± 0.266
2.669GluAsp: 2.669 ± 0.43
3.353GluGlu: 3.353 ± 0.608
1.984GluPhe: 1.984 ± 0.393
3.49GluGly: 3.49 ± 0.435
1.369GluHis: 1.369 ± 0.29
3.353GluIle: 3.353 ± 0.432
1.711GluLys: 1.711 ± 0.617
6.09GluLeu: 6.09 ± 0.78
1.779GluMet: 1.779 ± 0.345
1.505GluAsn: 1.505 ± 0.352
3.49GluPro: 3.49 ± 0.754
2.669GluGln: 2.669 ± 0.465
5.679GluArg: 5.679 ± 0.505
3.627GluSer: 3.627 ± 0.524
3.558GluThr: 3.558 ± 0.427
5.406GluVal: 5.406 ± 0.638
1.369GluTrp: 1.369 ± 0.376
1.711GluTyr: 1.711 ± 0.348
0.0GluXaa: 0.0 ± 0.0
Phe
3.49PheAla: 3.49 ± 0.496
0.205PheCys: 0.205 ± 0.103
3.011PheAsp: 3.011 ± 0.527
1.779PheGlu: 1.779 ± 0.431
0.547PhePhe: 0.547 ± 0.134
3.285PheGly: 3.285 ± 0.5
0.411PheHis: 0.411 ± 0.168
0.753PheIle: 0.753 ± 0.211
0.684PheLys: 0.684 ± 0.18
1.369PheLeu: 1.369 ± 0.291
0.411PheMet: 0.411 ± 0.137
0.616PheAsn: 0.616 ± 0.24
1.026PhePro: 1.026 ± 0.257
0.616PheGln: 0.616 ± 0.177
2.053PheArg: 2.053 ± 0.37
1.916PheSer: 1.916 ± 0.481
1.437PheThr: 1.437 ± 0.319
1.711PheVal: 1.711 ± 0.278
0.958PheTrp: 0.958 ± 0.278
0.479PheTyr: 0.479 ± 0.184
0.0PheXaa: 0.0 ± 0.0
Gly
9.854GlyAla: 9.854 ± 0.834
0.616GlyCys: 0.616 ± 0.252
5.679GlyAsp: 5.679 ± 0.66
5.337GlyGlu: 5.337 ± 0.499
2.532GlyPhe: 2.532 ± 0.428
9.032GlyGly: 9.032 ± 1.249
1.505GlyHis: 1.505 ± 0.37
4.448GlyIle: 4.448 ± 0.561
3.148GlyLys: 3.148 ± 0.385
7.39GlyLeu: 7.39 ± 0.78
2.121GlyMet: 2.121 ± 0.387
2.053GlyAsn: 2.053 ± 0.405
4.379GlyPro: 4.379 ± 0.636
3.011GlyGln: 3.011 ± 0.406
5.406GlyArg: 5.406 ± 0.511
4.516GlySer: 4.516 ± 0.575
4.516GlyThr: 4.516 ± 0.538
6.637GlyVal: 6.637 ± 0.74
1.779GlyTrp: 1.779 ± 0.373
2.806GlyTyr: 2.806 ± 0.425
0.0GlyXaa: 0.0 ± 0.0
His
2.19HisAla: 2.19 ± 0.366
0.274HisCys: 0.274 ± 0.123
1.3HisAsp: 1.3 ± 0.359
0.958HisGlu: 0.958 ± 0.298
0.547HisPhe: 0.547 ± 0.171
2.19HisGly: 2.19 ± 0.39
0.547HisHis: 0.547 ± 0.235
0.479HisIle: 0.479 ± 0.231
0.479HisLys: 0.479 ± 0.166
1.848HisLeu: 1.848 ± 0.346
0.547HisMet: 0.547 ± 0.232
0.479HisAsn: 0.479 ± 0.19
1.505HisPro: 1.505 ± 0.338
0.958HisGln: 0.958 ± 0.283
1.505HisArg: 1.505 ± 0.347
0.958HisSer: 0.958 ± 0.337
1.026HisThr: 1.026 ± 0.247
1.711HisVal: 1.711 ± 0.377
0.547HisTrp: 0.547 ± 0.204
0.684HisTyr: 0.684 ± 0.233
0.0HisXaa: 0.0 ± 0.0
Ile
6.98IleAla: 6.98 ± 0.775
0.342IleCys: 0.342 ± 0.182
4.79IleAsp: 4.79 ± 0.584
4.174IleGlu: 4.174 ± 0.418
0.821IlePhe: 0.821 ± 0.247
4.174IleGly: 4.174 ± 0.441
1.026IleHis: 1.026 ± 0.308
1.642IleIle: 1.642 ± 0.291
0.753IleLys: 0.753 ± 0.208
2.121IleLeu: 2.121 ± 0.385
0.274IleMet: 0.274 ± 0.172
1.026IleAsn: 1.026 ± 0.264
3.353IlePro: 3.353 ± 0.541
1.711IleGln: 1.711 ± 0.367
3.627IleArg: 3.627 ± 0.537
3.079IleSer: 3.079 ± 0.493
2.532IleThr: 2.532 ± 0.404
3.627IleVal: 3.627 ± 0.538
0.89IleTrp: 0.89 ± 0.253
1.095IleTyr: 1.095 ± 0.337
0.0IleXaa: 0.0 ± 0.0
Lys
3.764LysAla: 3.764 ± 0.552
0.137LysCys: 0.137 ± 0.095
1.984LysAsp: 1.984 ± 0.368
1.505LysGlu: 1.505 ± 0.474
0.958LysPhe: 0.958 ± 0.34
1.574LysGly: 1.574 ± 0.299
0.547LysHis: 0.547 ± 0.18
1.3LysIle: 1.3 ± 0.301
0.411LysLys: 0.411 ± 0.176
1.779LysLeu: 1.779 ± 0.452
0.411LysMet: 0.411 ± 0.183
0.547LysAsn: 0.547 ± 0.234
2.121LysPro: 2.121 ± 0.475
0.821LysGln: 0.821 ± 0.255
2.806LysArg: 2.806 ± 0.42
1.505LysSer: 1.505 ± 0.274
1.984LysThr: 1.984 ± 0.428
2.121LysVal: 2.121 ± 0.389
0.616LysTrp: 0.616 ± 0.218
0.821LysTyr: 0.821 ± 0.245
0.0LysXaa: 0.0 ± 0.0
Leu
10.88LeuAla: 10.88 ± 0.904
0.479LeuCys: 0.479 ± 0.194
5.132LeuAsp: 5.132 ± 0.579
3.216LeuGlu: 3.216 ± 0.546
2.258LeuPhe: 2.258 ± 0.576
7.595LeuGly: 7.595 ± 0.665
1.369LeuHis: 1.369 ± 0.321
4.106LeuIle: 4.106 ± 0.395
1.642LeuLys: 1.642 ± 0.388
5.406LeuLeu: 5.406 ± 0.824
1.369LeuMet: 1.369 ± 0.272
1.642LeuAsn: 1.642 ± 0.263
4.379LeuPro: 4.379 ± 0.472
1.916LeuGln: 1.916 ± 0.34
6.295LeuArg: 6.295 ± 0.813
3.558LeuSer: 3.558 ± 0.412
6.022LeuThr: 6.022 ± 0.691
4.995LeuVal: 4.995 ± 0.682
1.437LeuTrp: 1.437 ± 0.282
1.505LeuTyr: 1.505 ± 0.267
0.0LeuXaa: 0.0 ± 0.0
Met
2.806MetAla: 2.806 ± 0.414
0.205MetCys: 0.205 ± 0.129
0.821MetAsp: 0.821 ± 0.271
0.89MetGlu: 0.89 ± 0.208
0.547MetPhe: 0.547 ± 0.167
1.095MetGly: 1.095 ± 0.303
0.547MetHis: 0.547 ± 0.216
0.89MetIle: 0.89 ± 0.224
1.095MetLys: 1.095 ± 0.256
1.505MetLeu: 1.505 ± 0.322
0.411MetMet: 0.411 ± 0.133
0.411MetAsn: 0.411 ± 0.146
1.232MetPro: 1.232 ± 0.326
0.547MetGln: 0.547 ± 0.247
1.232MetArg: 1.232 ± 0.318
1.779MetSer: 1.779 ± 0.245
2.6MetThr: 2.6 ± 0.434
0.684MetVal: 0.684 ± 0.2
0.205MetTrp: 0.205 ± 0.109
0.137MetTyr: 0.137 ± 0.102
0.0MetXaa: 0.0 ± 0.0
Asn
2.806AsnAla: 2.806 ± 0.389
0.137AsnCys: 0.137 ± 0.102
1.848AsnAsp: 1.848 ± 0.353
1.163AsnGlu: 1.163 ± 0.252
0.547AsnPhe: 0.547 ± 0.181
3.353AsnGly: 3.353 ± 0.482
0.684AsnHis: 0.684 ± 0.196
0.753AsnIle: 0.753 ± 0.24
0.821AsnLys: 0.821 ± 0.277
2.463AsnLeu: 2.463 ± 0.337
0.616AsnMet: 0.616 ± 0.201
0.547AsnAsn: 0.547 ± 0.173
2.258AsnPro: 2.258 ± 0.378
0.89AsnGln: 0.89 ± 0.291
2.053AsnArg: 2.053 ± 0.45
0.753AsnSer: 0.753 ± 0.203
2.395AsnThr: 2.395 ± 0.542
1.163AsnVal: 1.163 ± 0.338
0.753AsnTrp: 0.753 ± 0.265
0.89AsnTyr: 0.89 ± 0.282
0.0AsnXaa: 0.0 ± 0.0
Pro
6.911ProAla: 6.911 ± 0.763
0.958ProCys: 0.958 ± 0.354
5.132ProAsp: 5.132 ± 0.564
4.995ProGlu: 4.995 ± 0.604
1.3ProPhe: 1.3 ± 0.271
5.543ProGly: 5.543 ± 0.583
0.89ProHis: 0.89 ± 0.28
2.874ProIle: 2.874 ± 0.391
1.916ProLys: 1.916 ± 0.373
3.285ProLeu: 3.285 ± 0.481
0.684ProMet: 0.684 ± 0.202
2.121ProAsn: 2.121 ± 0.387
2.942ProPro: 2.942 ± 0.48
1.505ProGln: 1.505 ± 0.341
3.421ProArg: 3.421 ± 0.546
4.243ProSer: 4.243 ± 0.74
3.832ProThr: 3.832 ± 0.612
4.585ProVal: 4.585 ± 0.528
1.369ProTrp: 1.369 ± 0.307
0.821ProTyr: 0.821 ± 0.185
0.0ProXaa: 0.0 ± 0.0
Gln
4.858GlnAla: 4.858 ± 0.714
0.274GlnCys: 0.274 ± 0.133
0.89GlnAsp: 0.89 ± 0.277
1.505GlnGlu: 1.505 ± 0.308
1.026GlnPhe: 1.026 ± 0.249
2.327GlnGly: 2.327 ± 0.407
1.026GlnHis: 1.026 ± 0.284
2.327GlnIle: 2.327 ± 0.394
0.753GlnLys: 0.753 ± 0.348
2.6GlnLeu: 2.6 ± 0.389
1.369GlnMet: 1.369 ± 0.324
0.821GlnAsn: 0.821 ± 0.255
2.19GlnPro: 2.19 ± 0.342
2.669GlnGln: 2.669 ± 0.635
3.832GlnArg: 3.832 ± 0.677
2.121GlnSer: 2.121 ± 0.428
1.848GlnThr: 1.848 ± 0.318
1.916GlnVal: 1.916 ± 0.38
0.753GlnTrp: 0.753 ± 0.225
0.684GlnTyr: 0.684 ± 0.216
0.0GlnXaa: 0.0 ± 0.0
Arg
8.896ArgAla: 8.896 ± 0.795
0.753ArgCys: 0.753 ± 0.288
5.679ArgAsp: 5.679 ± 0.693
5.337ArgGlu: 5.337 ± 0.688
2.737ArgPhe: 2.737 ± 0.448
4.995ArgGly: 4.995 ± 0.551
1.574ArgHis: 1.574 ± 0.419
3.832ArgIle: 3.832 ± 0.544
3.353ArgLys: 3.353 ± 0.396
6.227ArgLeu: 6.227 ± 0.716
2.053ArgMet: 2.053 ± 0.325
1.916ArgAsn: 1.916 ± 0.388
4.311ArgPro: 4.311 ± 0.622
2.258ArgGln: 2.258 ± 0.367
6.774ArgArg: 6.774 ± 0.816
3.764ArgSer: 3.764 ± 0.587
3.969ArgThr: 3.969 ± 0.516
5.132ArgVal: 5.132 ± 0.639
1.505ArgTrp: 1.505 ± 0.317
1.369ArgTyr: 1.369 ± 0.263
0.0ArgXaa: 0.0 ± 0.0
Ser
6.227SerAla: 6.227 ± 0.684
0.547SerCys: 0.547 ± 0.211
3.969SerAsp: 3.969 ± 0.496
3.421SerGlu: 3.421 ± 0.459
0.958SerPhe: 0.958 ± 0.268
4.721SerGly: 4.721 ± 0.747
0.616SerHis: 0.616 ± 0.232
3.353SerIle: 3.353 ± 0.428
1.984SerLys: 1.984 ± 0.372
3.695SerLeu: 3.695 ± 0.493
1.095SerMet: 1.095 ± 0.266
1.3SerAsn: 1.3 ± 0.263
3.148SerPro: 3.148 ± 0.541
2.19SerGln: 2.19 ± 0.382
3.216SerArg: 3.216 ± 0.644
3.079SerSer: 3.079 ± 0.456
3.695SerThr: 3.695 ± 0.557
3.695SerVal: 3.695 ± 0.455
1.163SerTrp: 1.163 ± 0.3
1.163SerTyr: 1.163 ± 0.231
0.0SerXaa: 0.0 ± 0.0
Thr
6.706ThrAla: 6.706 ± 0.705
0.753ThrCys: 0.753 ± 0.249
4.448ThrAsp: 4.448 ± 0.544
2.6ThrGlu: 2.6 ± 0.412
2.19ThrPhe: 2.19 ± 0.425
6.706ThrGly: 6.706 ± 0.855
1.505ThrHis: 1.505 ± 0.363
4.516ThrIle: 4.516 ± 0.506
1.642ThrLys: 1.642 ± 0.413
5.337ThrLeu: 5.337 ± 0.63
0.89ThrMet: 0.89 ± 0.192
1.848ThrAsn: 1.848 ± 0.415
3.558ThrPro: 3.558 ± 0.406
2.258ThrGln: 2.258 ± 0.359
4.106ThrArg: 4.106 ± 0.641
4.379ThrSer: 4.379 ± 0.477
5.611ThrThr: 5.611 ± 0.809
4.243ThrVal: 4.243 ± 0.671
1.369ThrTrp: 1.369 ± 0.29
1.505ThrTyr: 1.505 ± 0.331
0.0ThrXaa: 0.0 ± 0.0
Val
6.774ValAla: 6.774 ± 0.769
1.232ValCys: 1.232 ± 0.392
4.995ValAsp: 4.995 ± 0.759
5.337ValGlu: 5.337 ± 0.654
1.848ValPhe: 1.848 ± 0.382
6.295ValGly: 6.295 ± 0.592
1.163ValHis: 1.163 ± 0.273
3.285ValIle: 3.285 ± 0.416
1.984ValLys: 1.984 ± 0.268
4.516ValLeu: 4.516 ± 0.73
0.753ValMet: 0.753 ± 0.32
2.19ValAsn: 2.19 ± 0.362
3.969ValPro: 3.969 ± 0.576
1.916ValGln: 1.916 ± 0.348
6.364ValArg: 6.364 ± 0.673
3.285ValSer: 3.285 ± 0.568
6.432ValThr: 6.432 ± 0.84
5.406ValVal: 5.406 ± 0.819
1.163ValTrp: 1.163 ± 0.247
1.505ValTyr: 1.505 ± 0.269
0.0ValXaa: 0.0 ± 0.0
Trp
2.395TrpAla: 2.395 ± 0.378
0.137TrpCys: 0.137 ± 0.102
1.369TrpAsp: 1.369 ± 0.352
1.026TrpGlu: 1.026 ± 0.236
0.547TrpPhe: 0.547 ± 0.19
0.958TrpGly: 0.958 ± 0.26
0.958TrpHis: 0.958 ± 0.262
0.479TrpIle: 0.479 ± 0.226
0.479TrpLys: 0.479 ± 0.182
1.574TrpLeu: 1.574 ± 0.271
0.205TrpMet: 0.205 ± 0.123
0.342TrpAsn: 0.342 ± 0.174
1.3TrpPro: 1.3 ± 0.395
0.616TrpGln: 0.616 ± 0.208
1.848TrpArg: 1.848 ± 0.367
1.574TrpSer: 1.574 ± 0.335
1.574TrpThr: 1.574 ± 0.362
1.369TrpVal: 1.369 ± 0.26
0.479TrpTrp: 0.479 ± 0.161
0.547TrpTyr: 0.547 ± 0.215
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.737TyrAla: 2.737 ± 0.484
0.411TyrCys: 0.411 ± 0.171
1.232TyrAsp: 1.232 ± 0.29
1.163TyrGlu: 1.163 ± 0.262
0.479TyrPhe: 0.479 ± 0.151
2.395TyrGly: 2.395 ± 0.334
0.479TyrHis: 0.479 ± 0.202
0.616TyrIle: 0.616 ± 0.182
0.411TyrLys: 0.411 ± 0.159
1.711TyrLeu: 1.711 ± 0.299
0.479TyrMet: 0.479 ± 0.175
0.753TyrAsn: 0.753 ± 0.258
1.3TyrPro: 1.3 ± 0.342
1.095TyrGln: 1.095 ± 0.259
2.053TyrArg: 2.053 ± 0.361
1.232TyrSer: 1.232 ± 0.296
1.369TyrThr: 1.369 ± 0.287
2.19TyrVal: 2.19 ± 0.341
0.411TyrTrp: 0.411 ± 0.144
0.753TyrTyr: 0.753 ± 0.203
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 74 proteins (14615 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski