Chitinophagaceae bacterium
Average proteome isoelectric point is 6.95
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3060 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4V2A0I6|A0A4V2A0I6_9BACT PKD domain-containing protein OS=Chitinophagaceae bacterium OX=1869212 GN=EOO08_15175 PE=4 SV=1
MM1 pKa = 7.85 TIRR4 pKa = 11.84 CAFWLARR11 pKa = 11.84 FEE13 pKa = 4.39 TEE15 pKa = 4.2 EE16 pKa = 3.76 EE17 pKa = 4.31 LINYY21 pKa = 9.03 IEE23 pKa = 4.14 YY24 pKa = 10.31 HH25 pKa = 6.48 HH26 pKa = 7.34 DD27 pKa = 3.31 AAGAASSPLDD37 pKa = 3.91 DD38 pKa = 4.36 VLGKK42 pKa = 9.87 PGFDD46 pKa = 3.46 EE47 pKa = 5.0 EE48 pKa = 4.61 FLEE51 pKa = 5.38 CVYY54 pKa = 11.08 TDD56 pKa = 4.3 ADD58 pKa = 3.9 DD59 pKa = 4.94 LLEE62 pKa = 4.17 EE63 pKa = 4.9 LEE65 pKa = 4.13 GFTYY69 pKa = 10.42 SEE71 pKa = 4.65 HH72 pKa = 8.09 YY73 pKa = 10.18 EE74 pKa = 4.03 DD75 pKa = 3.89 QLRR78 pKa = 11.84 EE79 pKa = 3.95 RR80 pKa = 11.84 LVSIGGNWNTLLMISGVEE98 pKa = 3.79 GDD100 pKa = 3.56 TNEE103 pKa = 4.12 WLFEE107 pKa = 4.19 NPFTGRR113 pKa = 11.84 PAEE116 pKa = 3.82 HH117 pKa = 6.63 LMFVGSFDD125 pKa = 4.7 LEE127 pKa = 4.23 EE128 pKa = 4.08 EE129 pKa = 4.28 AA130 pKa = 5.75
Molecular weight: 14.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.792
IPC2_protein 3.961
IPC_protein 3.897
Toseland 3.719
ProMoST 4.012
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.719
Rodwell 3.732
Grimsley 3.643
Solomon 3.834
Lehninger 3.795
Nozaki 3.961
DTASelect 4.088
Thurlkill 3.745
EMBOSS 3.732
Sillero 3.999
Patrickios 1.863
IPC_peptide 3.846
IPC2_peptide 3.986
IPC2.peptide.svr19 3.907
Protein with the highest isoelectric point:
>tr|A0A4Q5SZN9|A0A4Q5SZN9_9BACT Phosphoribosylglycinamide formyltransferase OS=Chitinophagaceae bacterium OX=1869212 GN=purN PE=3 SV=1
MM1 pKa = 7.01 FRR3 pKa = 11.84 CAARR7 pKa = 11.84 CRR9 pKa = 11.84 CTCAILRR16 pKa = 11.84 WRR18 pKa = 11.84 AARR21 pKa = 11.84 ANWYY25 pKa = 10.0 FMRR28 pKa = 11.84 IFMGKK33 pKa = 7.13 TAVCGRR39 pKa = 11.84 LTWYY43 pKa = 10.57 VNRR46 pKa = 11.84 LLMRR50 pKa = 11.84 HH51 pKa = 6.38 FILYY55 pKa = 10.51 LLLLGTALPAAAQEE69 pKa = 4.44 TPKK72 pKa = 10.39 PATQSEE78 pKa = 5.12 TPQSADD84 pKa = 2.9 EE85 pKa = 4.47 DD86 pKa = 3.99 AAPGLKK92 pKa = 9.76 ISDD95 pKa = 4.05 RR96 pKa = 11.84 KK97 pKa = 10.33 PMFGIASYY105 pKa = 10.66 YY106 pKa = 10.14 AEE108 pKa = 4.58 KK109 pKa = 10.81 FNGRR113 pKa = 11.84 LTANGATYY121 pKa = 9.86 WSHH124 pKa = 6.72 KK125 pKa = 9.76 MSAACNRR132 pKa = 11.84 VPLGTWIRR140 pKa = 11.84 VTNLRR145 pKa = 11.84 NKK147 pKa = 10.2 RR148 pKa = 11.84 SIVLQVNDD156 pKa = 4.25 RR157 pKa = 11.84 LHH159 pKa = 6.66 PKK161 pKa = 9.7 NKK163 pKa = 9.66 RR164 pKa = 11.84 VVDD167 pKa = 4.58 LSRR170 pKa = 11.84 VAAQKK175 pKa = 10.5 LGYY178 pKa = 9.16 IGYY181 pKa = 10.03 GLTQVKK187 pKa = 9.78 VEE189 pKa = 3.88 ILVRR193 pKa = 11.84 KK194 pKa = 10.03 AGG196 pKa = 3.42
Molecular weight: 22.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.342
IPC2_protein 9.809
IPC_protein 10.511
Toseland 10.599
ProMoST 10.292
Dawson 10.73
Bjellqvist 10.438
Wikipedia 10.921
Rodwell 11.008
Grimsley 10.789
Solomon 10.804
Lehninger 10.774
Nozaki 10.599
DTASelect 10.423
Thurlkill 10.613
EMBOSS 10.994
Sillero 10.657
Patrickios 10.701
IPC_peptide 10.804
IPC2_peptide 9.575
IPC2.peptide.svr19 8.404
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3060
0
3060
1114154
23
5954
364.1
40.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.456 ± 0.059
0.913 ± 0.02
5.022 ± 0.035
5.338 ± 0.069
4.621 ± 0.032
7.78 ± 0.057
1.97 ± 0.031
5.444 ± 0.033
4.628 ± 0.056
9.779 ± 0.077
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.091 ± 0.024
4.549 ± 0.053
4.436 ± 0.03
3.905 ± 0.027
5.712 ± 0.06
6.341 ± 0.068
6.178 ± 0.113
6.923 ± 0.039
1.324 ± 0.021
3.592 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here