Carboxylicivirga sp. M1479
Average proteome isoelectric point is 6.29
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4095 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A553FRB2|A0A553FRB2_9BACT Uncharacterized protein OS=Carboxylicivirga sp. M1479 OX=2594476 GN=FNN09_20845 PE=4 SV=1
MM1 pKa = 7.46 LLVLVVAFSACKK13 pKa = 10.47 DD14 pKa = 4.06 DD15 pKa = 6.39 DD16 pKa = 5.94 DD17 pKa = 7.39 DD18 pKa = 5.17 DD19 pKa = 6.06 APTTGIIKK27 pKa = 8.81 GTVTDD32 pKa = 3.8 AANNEE37 pKa = 4.24 TLSDD41 pKa = 3.64 VSIMVFDD48 pKa = 3.68 ATTNEE53 pKa = 4.62 TIGEE57 pKa = 4.44 TIMSGADD64 pKa = 3.05 GMYY67 pKa = 10.43 SVTLDD72 pKa = 3.24 PGTYY76 pKa = 9.17 YY77 pKa = 11.18 LKK79 pKa = 10.73 LFKK82 pKa = 10.18 QGYY85 pKa = 8.35 NNNPPAGVPPLNISVSEE102 pKa = 4.2 GSEE105 pKa = 4.07 SVNDD109 pKa = 4.19 FKK111 pKa = 11.16 MQASEE116 pKa = 4.12 VLDD119 pKa = 4.18 GGSLTGKK126 pKa = 10.42 VMLGSNGVQGAYY138 pKa = 10.67 VLVTSGTMGYY148 pKa = 9.85 SAVSDD153 pKa = 3.52 KK154 pKa = 11.19 DD155 pKa = 3.32 GHH157 pKa = 5.5 FFIYY161 pKa = 10.07 NVPAATYY168 pKa = 8.95 TLTAYY173 pKa = 9.78 KK174 pKa = 9.76 INHH177 pKa = 6.53 NATPLSVTVTADD189 pKa = 2.98 ANTEE193 pKa = 4.23 GNVNLTEE200 pKa = 4.4 GASTSLSGTLTFLATEE216 pKa = 4.43 GKK218 pKa = 9.86 DD219 pKa = 3.38 VNVNLLHH226 pKa = 6.5 PTTNEE231 pKa = 4.46 PIPGLVDD238 pKa = 3.16 MSEE241 pKa = 3.94 NMSYY245 pKa = 10.71 SLSGIPDD252 pKa = 3.2 GDD254 pKa = 4.05 YY255 pKa = 10.8 LVKK258 pKa = 10.82 ASFEE262 pKa = 4.0 NDD264 pKa = 3.31 TYY266 pKa = 11.73 VVDD269 pKa = 4.53 PDD271 pKa = 4.17 WIIKK275 pKa = 10.25 NGEE278 pKa = 3.96 PMLTANGSAVVIDD291 pKa = 4.78 FSLTNAVTLNSPTNPMSTTVPIEE314 pKa = 4.02 IASTTPTFEE323 pKa = 3.77 WTSYY327 pKa = 10.8 SSSSDD332 pKa = 3.46 YY333 pKa = 11.17 VIEE336 pKa = 4.38 VSDD339 pKa = 4.22 ANGNVIWGGINDD351 pKa = 4.43 GVDD354 pKa = 3.42 PPLKK358 pKa = 10.55 NITIPSSTTSIAYY371 pKa = 10.23 NSDD374 pKa = 2.73 GMATIPNLEE383 pKa = 3.98 YY384 pKa = 10.94 DD385 pKa = 3.62 VVYY388 pKa = 10.11 RR389 pKa = 11.84 WKK391 pKa = 10.4 IYY393 pKa = 10.05 ASKK396 pKa = 11.26 NNVQEE401 pKa = 3.85 PSGWNLISVSEE412 pKa = 4.09 DD413 pKa = 3.12 QMGLFIVKK421 pKa = 8.96 EE422 pKa = 4.08 QQQ424 pKa = 2.75
Molecular weight: 45.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.709
IPC2_protein 3.821
IPC_protein 3.834
Toseland 3.617
ProMoST 3.999
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.757
Rodwell 3.656
Grimsley 3.528
Solomon 3.821
Lehninger 3.77
Nozaki 3.935
DTASelect 4.177
Thurlkill 3.668
EMBOSS 3.77
Sillero 3.961
Patrickios 0.998
IPC_peptide 3.821
IPC2_peptide 3.935
IPC2.peptide.svr19 3.833
Protein with the highest isoelectric point:
>tr|A0A553GPZ8|A0A553GPZ8_9BACT Uncharacterized protein OS=Carboxylicivirga sp. M1479 OX=2594476 GN=FNN09_06090 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNRR10 pKa = 11.84 KK11 pKa = 8.89 RR12 pKa = 11.84 RR13 pKa = 11.84 NKK15 pKa = 9.89 HH16 pKa = 3.93 GFRR19 pKa = 11.84 EE20 pKa = 4.27 RR21 pKa = 11.84 MASANGRR28 pKa = 11.84 KK29 pKa = 9.12 VLASRR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.05 GRR39 pKa = 11.84 KK40 pKa = 8.34 KK41 pKa = 10.71 LSVSSEE47 pKa = 3.93 KK48 pKa = 10.19 RR49 pKa = 11.84 HH50 pKa = 5.73 KK51 pKa = 10.69 AA52 pKa = 3.17
Molecular weight: 6.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.329
IPC2_protein 10.716
IPC_protein 12.266
Toseland 12.442
ProMoST 12.925
Dawson 12.442
Bjellqvist 12.427
Wikipedia 12.91
Rodwell 12.252
Grimsley 12.486
Solomon 12.939
Lehninger 12.837
Nozaki 12.442
DTASelect 12.427
Thurlkill 12.442
EMBOSS 12.939
Sillero 12.442
Patrickios 11.974
IPC_peptide 12.939
IPC2_peptide 11.915
IPC2.peptide.svr19 9.063
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4095
0
4095
1561341
34
6495
381.3
42.97
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.626 ± 0.034
0.875 ± 0.013
5.818 ± 0.032
6.588 ± 0.031
4.731 ± 0.026
6.632 ± 0.046
1.988 ± 0.018
7.39 ± 0.035
7.009 ± 0.058
9.221 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.509 ± 0.019
5.837 ± 0.036
3.396 ± 0.021
3.736 ± 0.023
3.697 ± 0.026
6.697 ± 0.035
5.305 ± 0.045
6.522 ± 0.035
1.182 ± 0.014
4.242 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here