Nocardia nova SH22a

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Nocardiaceae; Nocardia; Nocardia nova

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7577 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W5TSL1|W5TSL1_9NOCA Uncharacterized protein OS=Nocardia nova SH22a OX=1415166 GN=NONO_c75470 PE=4 SV=1
MM1 pKa = 7.2TPNAILEE8 pKa = 4.76FILDD12 pKa = 4.13LLRR15 pKa = 11.84DD16 pKa = 3.52HH17 pKa = 6.87EE18 pKa = 4.38AAVGYY23 pKa = 8.81CANPAEE29 pKa = 4.5SLCAAGLTAVTPEE42 pKa = 5.11DD43 pKa = 3.51IAAVAPMVAEE53 pKa = 4.18SALVSGGSQLAAIVAAGGGASGTAGLAGGAATTTEE88 pKa = 4.27ANLGTGAALGAATGIAAGTGLDD110 pKa = 3.64LGAGLGLGANAGIGGDD126 pKa = 3.74LGAGLGAAAAVGAGLEE142 pKa = 4.21AGLTAGLGALTGVAVGLGGALSGSGQTGADD172 pKa = 3.6LSTGFQAATEE182 pKa = 4.24TFADD186 pKa = 3.57AGANLSTGIEE196 pKa = 3.97AGINAATTGLADD208 pKa = 3.97ASTGLTAGVSGLNAEE223 pKa = 4.6LNGALGGAAEE233 pKa = 4.54AGAGLGAEE241 pKa = 4.6LGGALGGAAAAGAGLGAGLGGALGSVAQAGAGVGAGLSGALNGAAQAGAGLGGALGAVAGDD302 pKa = 3.89GAGLGAEE309 pKa = 4.56LGGALGGAAAAGAGLGAGLGGAAQAGAGLGAQLGGALGGAAEE351 pKa = 4.54AGAGLGAEE359 pKa = 4.53LGGASSGAAQAGAGLGAHH377 pKa = 7.18DD378 pKa = 5.53AGDD381 pKa = 4.23LSASLSGGATAGVQSAGDD399 pKa = 3.49VSGTLSGVAGSGLRR413 pKa = 11.84SVGDD417 pKa = 3.54LSGDD421 pKa = 3.32AAAGVHH427 pKa = 5.66SAGDD431 pKa = 3.5VSSALSGTGASGLEE445 pKa = 4.16SGLGSAGSLSSSLSGSADD463 pKa = 3.48SALHH467 pKa = 6.06TGTEE471 pKa = 4.3VSAGLSGLGTNLSGSADD488 pKa = 3.7SGVHH492 pKa = 5.85GGTDD496 pKa = 3.31VSSALSDD503 pKa = 3.36TASAGLHH510 pKa = 5.58GAGEE514 pKa = 4.37VSSAVSGTVGGDD526 pKa = 3.16LGSVDD531 pKa = 3.6SAATHH536 pKa = 5.9GLGVDD541 pKa = 3.8SSVGTPVSGTWSGVDD556 pKa = 3.34VSHH559 pKa = 7.35AFGSGVGADD568 pKa = 3.4LSGNFGGTGSGIDD581 pKa = 3.44SALSAGGHH589 pKa = 5.92GALDD593 pKa = 3.62SGIDD597 pKa = 3.72TSGHH601 pKa = 5.14TDD603 pKa = 2.83AGLFGDD609 pKa = 4.14THH611 pKa = 5.71TTTVTDD617 pKa = 3.1THH619 pKa = 6.36VAGDD623 pKa = 3.63VTGGLLHH630 pKa = 7.18

Molecular weight: 54.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W5T6I0|W5T6I0_9NOCA Superoxide dismutase OS=Nocardia nova SH22a OX=1415166 GN=sodA PE=3 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVSARR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 9.72GRR42 pKa = 11.84ASLTAA47 pKa = 4.1

Molecular weight: 5.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7577

0

7577

2480242

12

16761

327.3

35.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.331 ± 0.04

0.772 ± 0.01

6.375 ± 0.025

5.383 ± 0.029

2.933 ± 0.016

9.034 ± 0.028

2.281 ± 0.013

4.214 ± 0.016

1.819 ± 0.021

9.791 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.92 ± 0.012

1.99 ± 0.016

5.995 ± 0.027

2.799 ± 0.017

7.918 ± 0.029

5.308 ± 0.02

6.192 ± 0.016

8.406 ± 0.03

1.463 ± 0.012

2.076 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski