Amino acid dipepetide frequency for Nocardia nova SH22a

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
21.534AlaAla: 21.534 ± 0.145
0.949AlaCys: 0.949 ± 0.021
9.173AlaAsp: 9.173 ± 0.08
8.636AlaGlu: 8.636 ± 0.067
3.298AlaPhe: 3.298 ± 0.036
12.421AlaGly: 12.421 ± 0.092
2.852AlaHis: 2.852 ± 0.038
5.025AlaIle: 5.025 ± 0.052
2.457AlaLys: 2.457 ± 0.046
13.018AlaLeu: 13.018 ± 0.097
2.679AlaMet: 2.679 ± 0.034
2.251AlaAsn: 2.251 ± 0.035
6.728AlaPro: 6.728 ± 0.075
3.906AlaGln: 3.906 ± 0.041
9.67AlaArg: 9.67 ± 0.073
5.346AlaSer: 5.346 ± 0.061
7.593AlaThr: 7.593 ± 0.056
11.912AlaVal: 11.912 ± 0.089
1.562AlaTrp: 1.562 ± 0.027
2.299AlaTyr: 2.299 ± 0.037
0.0AlaXaa: 0.0 ± 0.0
Cys
1.126CysAla: 1.126 ± 0.022
0.083CysCys: 0.083 ± 0.007
0.508CysAsp: 0.508 ± 0.018
0.396CysGlu: 0.396 ± 0.013
0.211CysPhe: 0.211 ± 0.009
0.895CysGly: 0.895 ± 0.021
0.173CysHis: 0.173 ± 0.009
0.21CysIle: 0.21 ± 0.01
0.113CysLys: 0.113 ± 0.008
0.675CysLeu: 0.675 ± 0.018
0.1CysMet: 0.1 ± 0.006
0.14CysAsn: 0.14 ± 0.008
0.495CysPro: 0.495 ± 0.018
0.154CysGln: 0.154 ± 0.009
0.59CysArg: 0.59 ± 0.02
0.458CysSer: 0.458 ± 0.015
0.44CysThr: 0.44 ± 0.013
0.643CysVal: 0.643 ± 0.014
0.115CysTrp: 0.115 ± 0.007
0.196CysTyr: 0.196 ± 0.009
0.0CysXaa: 0.0 ± 0.0
Asp
7.657AspAla: 7.657 ± 0.071
0.462AspCys: 0.462 ± 0.014
3.992AspAsp: 3.992 ± 0.044
3.847AspGlu: 3.847 ± 0.039
1.785AspPhe: 1.785 ± 0.032
5.762AspGly: 5.762 ± 0.057
1.605AspHis: 1.605 ± 0.03
2.84AspIle: 2.84 ± 0.036
1.137AspLys: 1.137 ± 0.028
6.486AspLeu: 6.486 ± 0.068
0.986AspMet: 0.986 ± 0.021
1.212AspAsn: 1.212 ± 0.023
4.943AspPro: 4.943 ± 0.045
1.73AspGln: 1.73 ± 0.031
5.639AspArg: 5.639 ± 0.057
3.191AspSer: 3.191 ± 0.038
3.577AspThr: 3.577 ± 0.045
4.542AspVal: 4.542 ± 0.048
0.986AspTrp: 0.986 ± 0.021
1.371AspTyr: 1.371 ± 0.021
0.0AspXaa: 0.0 ± 0.0
Glu
5.831GluAla: 5.831 ± 0.058
0.374GluCys: 0.374 ± 0.013
2.281GluAsp: 2.281 ± 0.036
2.338GluGlu: 2.338 ± 0.038
2.097GluPhe: 2.097 ± 0.028
3.164GluGly: 3.164 ± 0.039
1.613GluHis: 1.613 ± 0.029
2.842GluIle: 2.842 ± 0.036
1.138GluLys: 1.138 ± 0.027
7.04GluLeu: 7.04 ± 0.06
1.024GluMet: 1.024 ± 0.02
1.095GluAsn: 1.095 ± 0.024
3.303GluPro: 3.303 ± 0.048
2.411GluGln: 2.411 ± 0.038
4.91GluArg: 4.91 ± 0.047
3.096GluSer: 3.096 ± 0.035
2.864GluThr: 2.864 ± 0.033
4.322GluVal: 4.322 ± 0.046
0.843GluTrp: 0.843 ± 0.02
1.246GluTyr: 1.246 ± 0.024
0.0GluXaa: 0.0 ± 0.0
Phe
4.118PheAla: 4.118 ± 0.046
0.275PheCys: 0.275 ± 0.011
2.609PheAsp: 2.609 ± 0.043
1.616PheGlu: 1.616 ± 0.032
0.835PhePhe: 0.835 ± 0.021
3.562PheGly: 3.562 ± 0.042
0.65PheHis: 0.65 ± 0.016
0.881PheIle: 0.881 ± 0.02
0.375PheLys: 0.375 ± 0.014
2.47PheLeu: 2.47 ± 0.037
0.41PheMet: 0.41 ± 0.014
0.527PheAsn: 0.527 ± 0.015
1.413PhePro: 1.413 ± 0.027
0.686PheGln: 0.686 ± 0.02
1.911PheArg: 1.911 ± 0.031
1.447PheSer: 1.447 ± 0.026
2.055PheThr: 2.055 ± 0.03
2.473PheVal: 2.473 ± 0.038
0.416PheTrp: 0.416 ± 0.014
0.606PheTyr: 0.606 ± 0.017
0.0PheXaa: 0.0 ± 0.0
Gly
10.795GlyAla: 10.795 ± 0.084
0.803GlyCys: 0.803 ± 0.019
5.142GlyAsp: 5.142 ± 0.055
4.61GlyGlu: 4.61 ± 0.047
3.053GlyPhe: 3.053 ± 0.04
7.962GlyGly: 7.962 ± 0.078
2.155GlyHis: 2.155 ± 0.036
4.254GlyIle: 4.254 ± 0.047
2.121GlyLys: 2.121 ± 0.031
8.38GlyLeu: 8.38 ± 0.072
2.111GlyMet: 2.111 ± 0.03
1.919GlyAsn: 1.919 ± 0.038
4.954GlyPro: 4.954 ± 0.05
2.484GlyGln: 2.484 ± 0.042
7.028GlyArg: 7.028 ± 0.061
5.432GlySer: 5.432 ± 0.058
5.793GlyThr: 5.793 ± 0.059
7.334GlyVal: 7.334 ± 0.061
1.604GlyTrp: 1.604 ± 0.024
2.403GlyTyr: 2.403 ± 0.034
0.0GlyXaa: 0.0 ± 0.0
His
2.589HisAla: 2.589 ± 0.033
0.212HisCys: 0.212 ± 0.01
1.461HisAsp: 1.461 ± 0.031
1.102HisGlu: 1.102 ± 0.024
0.677HisPhe: 0.677 ± 0.018
2.238HisGly: 2.238 ± 0.035
0.695HisHis: 0.695 ± 0.021
0.92HisIle: 0.92 ± 0.02
0.323HisLys: 0.323 ± 0.013
2.179HisLeu: 2.179 ± 0.034
0.39HisMet: 0.39 ± 0.012
0.511HisAsn: 0.511 ± 0.015
1.845HisPro: 1.845 ± 0.029
0.553HisGln: 0.553 ± 0.016
2.163HisArg: 2.163 ± 0.034
1.186HisSer: 1.186 ± 0.023
1.415HisThr: 1.415 ± 0.026
1.457HisVal: 1.457 ± 0.024
0.357HisTrp: 0.357 ± 0.012
0.536HisTyr: 0.536 ± 0.015
0.0HisXaa: 0.0 ± 0.0
Ile
6.53IleAla: 6.53 ± 0.056
0.364IleCys: 0.364 ± 0.013
3.443IleAsp: 3.443 ± 0.042
2.64IleGlu: 2.64 ± 0.035
1.036IlePhe: 1.036 ± 0.022
4.682IleGly: 4.682 ± 0.045
0.817IleHis: 0.817 ± 0.018
1.276IleIle: 1.276 ± 0.027
0.62IleLys: 0.62 ± 0.017
3.189IleLeu: 3.189 ± 0.037
0.598IleMet: 0.598 ± 0.016
0.852IleAsn: 0.852 ± 0.021
2.426IlePro: 2.426 ± 0.034
0.848IleGln: 0.848 ± 0.019
2.925IleArg: 2.925 ± 0.033
2.192IleSer: 2.192 ± 0.026
2.749IleThr: 2.749 ± 0.036
3.773IleVal: 3.773 ± 0.044
0.469IleTrp: 0.469 ± 0.013
0.716IleTyr: 0.716 ± 0.018
0.0IleXaa: 0.0 ± 0.0
Lys
2.172LysAla: 2.172 ± 0.038
0.096LysCys: 0.096 ± 0.006
0.891LysAsp: 0.891 ± 0.023
0.752LysGlu: 0.752 ± 0.022
0.562LysPhe: 0.562 ± 0.018
1.274LysGly: 1.274 ± 0.026
0.387LysHis: 0.387 ± 0.015
0.886LysIle: 0.886 ± 0.02
0.529LysLys: 0.529 ± 0.02
1.897LysLeu: 1.897 ± 0.033
0.413LysMet: 0.413 ± 0.015
0.435LysAsn: 0.435 ± 0.017
1.223LysPro: 1.223 ± 0.028
0.698LysGln: 0.698 ± 0.019
1.332LysArg: 1.332 ± 0.027
1.142LysSer: 1.142 ± 0.024
1.119LysThr: 1.119 ± 0.025
1.628LysVal: 1.628 ± 0.032
0.279LysTrp: 0.279 ± 0.012
0.474LysTyr: 0.474 ± 0.015
0.0LysXaa: 0.0 ± 0.0
Leu
13.918LeuAla: 13.918 ± 0.092
0.818LeuCys: 0.818 ± 0.019
6.928LeuAsp: 6.928 ± 0.073
4.575LeuGlu: 4.575 ± 0.041
2.717LeuPhe: 2.717 ± 0.038
8.821LeuGly: 8.821 ± 0.068
2.135LeuHis: 2.135 ± 0.03
4.136LeuIle: 4.136 ± 0.047
1.331LeuLys: 1.331 ± 0.031
9.693LeuLeu: 9.693 ± 0.08
1.53LeuMet: 1.53 ± 0.029
1.799LeuAsn: 1.799 ± 0.03
5.99LeuPro: 5.99 ± 0.063
2.199LeuGln: 2.199 ± 0.03
8.374LeuArg: 8.374 ± 0.068
5.596LeuSer: 5.596 ± 0.051
6.507LeuThr: 6.507 ± 0.055
7.98LeuVal: 7.98 ± 0.077
1.186LeuTrp: 1.186 ± 0.023
1.674LeuTyr: 1.674 ± 0.024
0.0LeuXaa: 0.0 ± 0.0
Met
2.247MetAla: 2.247 ± 0.031
0.162MetCys: 0.162 ± 0.009
0.887MetAsp: 0.887 ± 0.019
0.631MetGlu: 0.631 ± 0.018
0.589MetPhe: 0.589 ± 0.015
1.362MetGly: 1.362 ± 0.024
0.409MetHis: 0.409 ± 0.013
0.917MetIle: 0.917 ± 0.017
0.409MetLys: 0.409 ± 0.014
1.931MetLeu: 1.931 ± 0.033
0.397MetMet: 0.397 ± 0.012
0.525MetAsn: 0.525 ± 0.016
1.221MetPro: 1.221 ± 0.024
0.5MetGln: 0.5 ± 0.013
1.59MetArg: 1.59 ± 0.026
1.584MetSer: 1.584 ± 0.03
1.69MetThr: 1.69 ± 0.024
1.553MetVal: 1.553 ± 0.027
0.237MetTrp: 0.237 ± 0.01
0.353MetTyr: 0.353 ± 0.015
0.0MetXaa: 0.0 ± 0.0
Asn
2.332AsnAla: 2.332 ± 0.034
0.17AsnCys: 0.17 ± 0.009
1.033AsnAsp: 1.033 ± 0.022
0.86AsnGlu: 0.86 ± 0.022
0.606AsnPhe: 0.606 ± 0.014
1.958AsnGly: 1.958 ± 0.035
0.429AsnHis: 0.429 ± 0.013
0.895AsnIle: 0.895 ± 0.02
0.401AsnLys: 0.401 ± 0.015
1.928AsnLeu: 1.928 ± 0.031
0.382AsnMet: 0.382 ± 0.012
0.483AsnAsn: 0.483 ± 0.015
1.639AsnPro: 1.639 ± 0.029
0.571AsnGln: 0.571 ± 0.019
1.517AsnArg: 1.517 ± 0.025
1.205AsnSer: 1.205 ± 0.025
1.263AsnThr: 1.263 ± 0.027
1.401AsnVal: 1.401 ± 0.025
0.323AsnTrp: 0.323 ± 0.013
0.507AsnTyr: 0.507 ± 0.015
0.0AsnXaa: 0.0 ± 0.0
Pro
8.256ProAla: 8.256 ± 0.078
0.282ProCys: 0.282 ± 0.012
4.837ProAsp: 4.837 ± 0.049
4.19ProGlu: 4.19 ± 0.047
1.543ProPhe: 1.543 ± 0.03
6.432ProGly: 6.432 ± 0.07
1.288ProHis: 1.288 ± 0.026
2.23ProIle: 2.23 ± 0.031
1.176ProLys: 1.176 ± 0.023
4.876ProLeu: 4.876 ± 0.056
1.147ProMet: 1.147 ± 0.022
1.186ProAsn: 1.186 ± 0.023
3.388ProPro: 3.388 ± 0.058
1.857ProGln: 1.857 ± 0.033
3.806ProArg: 3.806 ± 0.047
2.947ProSer: 2.947 ± 0.037
3.209ProThr: 3.209 ± 0.041
5.349ProVal: 5.349 ± 0.06
0.829ProTrp: 0.829 ± 0.02
1.125ProTyr: 1.125 ± 0.024
0.0ProXaa: 0.0 ± 0.0
Gln
3.374GlnAla: 3.374 ± 0.041
0.198GlnCys: 0.198 ± 0.008
1.166GlnAsp: 1.166 ± 0.022
1.089GlnGlu: 1.089 ± 0.021
0.858GlnPhe: 0.858 ± 0.022
1.903GlnGly: 1.903 ± 0.036
0.679GlnHis: 0.679 ± 0.017
1.388GlnIle: 1.388 ± 0.027
0.528GlnLys: 0.528 ± 0.016
3.321GlnLeu: 3.321 ± 0.043
0.586GlnMet: 0.586 ± 0.017
0.575GlnAsn: 0.575 ± 0.019
1.816GlnPro: 1.816 ± 0.039
1.349GlnGln: 1.349 ± 0.032
2.771GlnArg: 2.771 ± 0.035
1.349GlnSer: 1.349 ± 0.022
1.361GlnThr: 1.361 ± 0.026
2.53GlnVal: 2.53 ± 0.038
0.553GlnTrp: 0.553 ± 0.015
0.597GlnTyr: 0.597 ± 0.017
0.0GlnXaa: 0.0 ± 0.0
Arg
9.456ArgAla: 9.456 ± 0.073
0.587ArgCys: 0.587 ± 0.016
4.652ArgAsp: 4.652 ± 0.049
4.41ArgGlu: 4.41 ± 0.043
2.505ArgPhe: 2.505 ± 0.039
5.523ArgGly: 5.523 ± 0.051
1.949ArgHis: 1.949 ± 0.031
4.129ArgIle: 4.129 ± 0.043
1.553ArgLys: 1.553 ± 0.033
7.928ArgLeu: 7.928 ± 0.069
2.084ArgMet: 2.084 ± 0.03
1.67ArgAsn: 1.67 ± 0.028
4.568ArgPro: 4.568 ± 0.051
2.245ArgGln: 2.245 ± 0.036
7.274ArgArg: 7.274 ± 0.077
4.298ArgSer: 4.298 ± 0.044
5.175ArgThr: 5.175 ± 0.042
5.755ArgVal: 5.755 ± 0.044
1.443ArgTrp: 1.443 ± 0.028
1.973ArgTyr: 1.973 ± 0.029
0.0ArgXaa: 0.0 ± 0.0
Ser
7.381SerAla: 7.381 ± 0.06
0.385SerCys: 0.385 ± 0.012
3.321SerAsp: 3.321 ± 0.041
2.693SerGlu: 2.693 ± 0.036
1.554SerPhe: 1.554 ± 0.023
6.304SerGly: 6.304 ± 0.062
1.001SerHis: 1.001 ± 0.021
2.107SerIle: 2.107 ± 0.029
1.012SerLys: 1.012 ± 0.023
4.468SerLeu: 4.468 ± 0.042
1.276SerMet: 1.276 ± 0.023
1.005SerAsn: 1.005 ± 0.024
2.982SerPro: 2.982 ± 0.04
1.298SerGln: 1.298 ± 0.024
3.693SerArg: 3.693 ± 0.047
2.887SerSer: 2.887 ± 0.042
3.32SerThr: 3.32 ± 0.04
4.361SerVal: 4.361 ± 0.044
0.878SerTrp: 0.878 ± 0.02
1.15SerTyr: 1.15 ± 0.021
0.0SerXaa: 0.0 ± 0.0
Thr
8.786ThrAla: 8.786 ± 0.072
0.412ThrCys: 0.412 ± 0.014
3.95ThrAsp: 3.95 ± 0.045
3.37ThrGlu: 3.37 ± 0.042
1.726ThrPhe: 1.726 ± 0.025
6.522ThrGly: 6.522 ± 0.052
1.222ThrHis: 1.222 ± 0.026
2.349ThrIle: 2.349 ± 0.031
1.011ThrLys: 1.011 ± 0.023
5.526ThrLeu: 5.526 ± 0.051
1.102ThrMet: 1.102 ± 0.022
1.072ThrAsn: 1.072 ± 0.024
3.98ThrPro: 3.98 ± 0.048
1.314ThrGln: 1.314 ± 0.025
3.979ThrArg: 3.979 ± 0.039
3.181ThrSer: 3.181 ± 0.038
4.198ThrThr: 4.198 ± 0.052
6.185ThrVal: 6.185 ± 0.055
0.8ThrTrp: 0.8 ± 0.021
1.232ThrTyr: 1.232 ± 0.023
0.0ThrXaa: 0.0 ± 0.0
Val
11.339ValAla: 11.339 ± 0.085
0.708ValCys: 0.708 ± 0.018
5.365ValAsp: 5.365 ± 0.049
4.343ValGlu: 4.343 ± 0.044
2.459ValPhe: 2.459 ± 0.033
6.557ValGly: 6.557 ± 0.059
1.902ValHis: 1.902 ± 0.028
3.518ValIle: 3.518 ± 0.043
1.301ValLys: 1.301 ± 0.025
8.898ValLeu: 8.898 ± 0.077
1.362ValMet: 1.362 ± 0.028
1.799ValAsn: 1.799 ± 0.03
5.061ValPro: 5.061 ± 0.062
1.963ValGln: 1.963 ± 0.026
6.698ValArg: 6.698 ± 0.054
4.427ValSer: 4.427 ± 0.045
5.505ValThr: 5.505 ± 0.052
8.318ValVal: 8.318 ± 0.077
1.038ValTrp: 1.038 ± 0.02
1.494ValTyr: 1.494 ± 0.029
0.0ValXaa: 0.0 ± 0.0
Trp
1.503TrpAla: 1.503 ± 0.026
0.169TrpCys: 0.169 ± 0.007
0.802TrpAsp: 0.802 ± 0.02
0.684TrpGlu: 0.684 ± 0.018
0.508TrpPhe: 0.508 ± 0.016
0.969TrpGly: 0.969 ± 0.019
0.369TrpHis: 0.369 ± 0.013
0.667TrpIle: 0.667 ± 0.017
0.338TrpLys: 0.338 ± 0.012
1.639TrpLeu: 1.639 ± 0.028
0.327TrpMet: 0.327 ± 0.01
0.422TrpAsn: 0.422 ± 0.012
0.752TrpPro: 0.752 ± 0.018
0.608TrpGln: 0.608 ± 0.016
1.262TrpArg: 1.262 ± 0.024
0.984TrpSer: 0.984 ± 0.022
0.936TrpThr: 0.936 ± 0.019
1.029TrpVal: 1.029 ± 0.022
0.337TrpTrp: 0.337 ± 0.012
0.323TrpTyr: 0.323 ± 0.012
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.365TyrAla: 2.365 ± 0.03
0.21TyrCys: 0.21 ± 0.009
1.31TyrAsp: 1.31 ± 0.027
1.051TyrGlu: 1.051 ± 0.024
0.714TyrPhe: 0.714 ± 0.019
2.025TyrGly: 2.025 ± 0.03
0.479TyrHis: 0.479 ± 0.014
0.671TyrIle: 0.671 ± 0.017
0.316TyrLys: 0.316 ± 0.013
2.366TyrLeu: 2.366 ± 0.03
0.294TyrMet: 0.294 ± 0.013
0.426TyrAsn: 0.426 ± 0.013
1.229TyrPro: 1.229 ± 0.025
0.616TyrGln: 0.616 ± 0.017
2.046TyrArg: 2.046 ± 0.033
1.128TyrSer: 1.128 ± 0.023
1.145TyrThr: 1.145 ± 0.022
1.508TyrVal: 1.508 ± 0.043
0.374TyrTrp: 0.374 ± 0.013
0.486TyrTyr: 0.486 ± 0.016
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 7577 proteins (2480242 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski