Asticcacaulis taihuensis
Average proteome isoelectric point is 6.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3569 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1G4PFH0|A0A1G4PFH0_9CAUL Probable cytosol aminopeptidase OS=Asticcacaulis taihuensis OX=260084 GN=pepA PE=3 SV=1
MM1 pKa = 7.94 RR2 pKa = 11.84 KK3 pKa = 9.49 HH4 pKa = 6.39 SLLTATALGLVLGSGGLGTQALAEE28 pKa = 4.3 TSISTATTTPLVTSSAGDD46 pKa = 3.47 LTVTSDD52 pKa = 3.65 GSITLTSGTAITVDD66 pKa = 3.76 SDD68 pKa = 3.32 NSLDD72 pKa = 3.92 FEE74 pKa = 4.8 GSIAMSGSEE83 pKa = 4.08 SSSTGILITDD93 pKa = 4.52 FPDD96 pKa = 3.3 RR97 pKa = 11.84 TKK99 pKa = 11.42 GLVLTGDD106 pKa = 3.21 ITVTDD111 pKa = 5.36 DD112 pKa = 3.31 YY113 pKa = 10.57 TASDD117 pKa = 3.8 TTSLDD122 pKa = 3.38 GTEE125 pKa = 5.26 DD126 pKa = 4.43 GYY128 pKa = 9.82 TDD130 pKa = 4.11 APWAEE135 pKa = 3.93 GTGRR139 pKa = 11.84 YY140 pKa = 9.12 GIHH143 pKa = 5.83 STGASPFVGDD153 pKa = 4.08 VYY155 pKa = 10.28 ITSDD159 pKa = 3.38 SVIDD163 pKa = 3.71 VEE165 pKa = 5.47 GNQSYY170 pKa = 10.57 GIRR173 pKa = 11.84 FEE175 pKa = 4.15 NQIKK179 pKa = 10.32 GAFTYY184 pKa = 10.5 DD185 pKa = 2.98 GAMTLIGDD193 pKa = 3.81 NSTGISLEE201 pKa = 3.9 KK202 pKa = 10.59 GVTGNVYY209 pKa = 10.49 LSGSVGVLGANASAITLAGDD229 pKa = 3.93 LGGNLIIDD237 pKa = 4.26 GSYY240 pKa = 10.77 SGTAYY245 pKa = 10.8 SSTSALTQAVYY256 pKa = 11.06 EE257 pKa = 4.1 NLIPANNLLQVGPLVSIAGNVANGVLFGASVTSTDD292 pKa = 5.16 DD293 pKa = 3.6 SNTDD297 pKa = 3.12 EE298 pKa = 5.92 DD299 pKa = 5.48 GDD301 pKa = 4.21 GLTDD305 pKa = 4.04 TVQSTASLTQYY316 pKa = 10.97 GSAPALAIGSASSDD330 pKa = 2.86 ITLGGLTYY338 pKa = 10.92 ASTAIDD344 pKa = 4.22 PPSVNYY350 pKa = 10.56 GLLNRR355 pKa = 11.84 GSIGAYY361 pKa = 9.15 GVHH364 pKa = 6.82 PGVNATALQIGGTGYY379 pKa = 7.61 PTTIEE384 pKa = 4.03 NGIGNAGSITASSYY398 pKa = 11.06 GGNATAISLLSGATTPRR415 pKa = 11.84 LDD417 pKa = 3.3 IDD419 pKa = 3.89 GTVTATTTRR428 pKa = 11.84 YY429 pKa = 7.8 LTSAEE434 pKa = 4.15 DD435 pKa = 3.74 ADD437 pKa = 4.28 GNTVYY442 pKa = 10.14 TVSNVGEE449 pKa = 4.05 ATAVSIASGATLPAINVAASSGIYY473 pKa = 10.01 ASSSGSTGSATAISDD488 pKa = 3.51 TSGTLTSISNSGTISATITASDD510 pKa = 4.3 DD511 pKa = 4.42 DD512 pKa = 5.21 GDD514 pKa = 4.18 GTTDD518 pKa = 4.07 TITGKK523 pKa = 8.83 ATAIDD528 pKa = 3.84 VSTNTTGVTITQTDD542 pKa = 3.51 NNATDD547 pKa = 3.62 SDD549 pKa = 4.22 DD550 pKa = 5.0 DD551 pKa = 3.91 EE552 pKa = 7.45 AIAAPYY558 pKa = 9.94 IYY560 pKa = 10.88 GNILFGSGNDD570 pKa = 3.73 TLSSSGGYY578 pKa = 9.63 IYY580 pKa = 11.2 GNVDD584 pKa = 3.06 YY585 pKa = 10.87 GAGTGSFSLTNDD597 pKa = 2.76 AVYY600 pKa = 10.42 LGKK603 pKa = 8.94 LTSSGDD609 pKa = 3.0 IAMDD613 pKa = 3.45 IDD615 pKa = 4.05 SGASAGLLAGSSVRR629 pKa = 11.84 MSTLHH634 pKa = 6.02 VGSDD638 pKa = 3.07 SSMALTLAVDD648 pKa = 4.27 TPTVPILSGSGAVAFDD664 pKa = 4.83 DD665 pKa = 4.72 GAKK668 pKa = 10.42 LYY670 pKa = 8.65 LTLDD674 pKa = 4.56 KK675 pKa = 10.78 ILSTPTSFTVLTGSSISLGDD695 pKa = 3.6 MTTSTLDD702 pKa = 3.22 GYY704 pKa = 10.55 IPYY707 pKa = 9.88 LYY709 pKa = 10.69 HH710 pKa = 7.96 SDD712 pKa = 3.35 LTLNDD717 pKa = 3.51 TDD719 pKa = 4.16 TVLTANFRR727 pKa = 11.84 LKK729 pKa = 9.33 TQGEE733 pKa = 4.2 AGYY736 pKa = 10.88 SSNQYY741 pKa = 9.52 AALMPVLVVVAEE753 pKa = 4.27 DD754 pKa = 3.13 SGARR758 pKa = 11.84 SSLLAATDD766 pKa = 3.52 KK767 pKa = 11.41 DD768 pKa = 4.61 AFDD771 pKa = 3.55 QVYY774 pKa = 9.77 NQYY777 pKa = 11.0 LPDD780 pKa = 3.63 YY781 pKa = 9.84 SGEE784 pKa = 4.05 NLISLSVGSASLNRR798 pKa = 11.84 SLSNLTLIPDD808 pKa = 4.06 NNGGQYY814 pKa = 9.34 WLQEE818 pKa = 3.52 YY819 pKa = 10.18 GYY821 pKa = 9.9 AINRR825 pKa = 11.84 AYY827 pKa = 11.08 SDD829 pKa = 3.34 TAGFKK834 pKa = 9.49 STGFSFAGGRR844 pKa = 11.84 EE845 pKa = 3.84 KK846 pKa = 10.4 QVYY849 pKa = 7.91 GNQMVGTYY857 pKa = 10.56 LSITSASPRR866 pKa = 11.84 DD867 pKa = 4.0 TFALAAEE874 pKa = 4.72 GMSNSDD880 pKa = 3.69 LTIGAYY886 pKa = 8.5 WRR888 pKa = 11.84 LNTSGLKK895 pKa = 9.94 AWLHH899 pKa = 6.25 AGAGYY904 pKa = 8.18 DD905 pKa = 3.55 TFKK908 pKa = 10.05 STRR911 pKa = 11.84 NILTTTVDD919 pKa = 3.52 HH920 pKa = 6.32 VATAKK925 pKa = 9.47 WDD927 pKa = 4.13 GYY929 pKa = 9.98 SVSAGTGASYY939 pKa = 11.52 DD940 pKa = 3.65 FLLGKK945 pKa = 9.13 WAVTPEE951 pKa = 3.95 VLADD955 pKa = 3.39 YY956 pKa = 10.28 YY957 pKa = 10.9 QLHH960 pKa = 6.67 EE961 pKa = 4.62 NDD963 pKa = 3.6 HH964 pKa = 6.07 TEE966 pKa = 4.13 SGGGDD971 pKa = 3.66 YY972 pKa = 11.07 FDD974 pKa = 4.31 LTVGARR980 pKa = 11.84 DD981 pKa = 3.52 GHH983 pKa = 6.41 LLSSTALLNLSYY995 pKa = 9.62 RR996 pKa = 11.84 TSFLKK1001 pKa = 10.51 PEE1003 pKa = 3.68 MWVGYY1008 pKa = 8.66 KK1009 pKa = 10.19 QNISATLPDD1018 pKa = 3.66 TVANFTGGDD1027 pKa = 3.46 SFTLVGGNIEE1037 pKa = 4.2 GGGPVAGFRR1046 pKa = 11.84 LSADD1050 pKa = 3.05 NPYY1053 pKa = 10.9 SYY1055 pKa = 11.11 FSIEE1059 pKa = 3.92 AEE1061 pKa = 4.07 YY1062 pKa = 11.03 QEE1064 pKa = 4.58 LPEE1067 pKa = 4.07 YY1068 pKa = 10.46 TNTSVSLRR1076 pKa = 11.84 TRR1078 pKa = 11.84 FQFF1081 pKa = 4.0
Molecular weight: 110.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.774
IPC2_protein 3.846
IPC_protein 3.897
Toseland 3.656
ProMoST 4.062
Dawson 3.897
Bjellqvist 4.05
Wikipedia 3.846
Rodwell 3.719
Grimsley 3.567
Solomon 3.897
Lehninger 3.859
Nozaki 3.999
DTASelect 4.304
Thurlkill 3.706
EMBOSS 3.859
Sillero 4.024
Patrickios 0.579
IPC_peptide 3.884
IPC2_peptide 3.999
IPC2.peptide.svr19 3.893
Protein with the highest isoelectric point:
>tr|A0A1G4PWI4|A0A1G4PWI4_9CAUL EF hand OS=Asticcacaulis taihuensis OX=260084 GN=SAMN02927928_0703 PE=4 SV=1
MM1 pKa = 7.29 KK2 pKa = 10.41 RR3 pKa = 11.84 SLTNRR8 pKa = 11.84 ATQVLDD14 pKa = 4.01 LLPFAQLLTRR24 pKa = 11.84 RR25 pKa = 11.84 PPSGKK30 pKa = 10.17 LLTAAALVNSPMANRR45 pKa = 11.84 LPPRR49 pKa = 11.84 LVGFAKK55 pKa = 10.26 IAVAALAVGSVIAGARR71 pKa = 11.84 QRR73 pKa = 11.84 PGFKK77 pKa = 8.66 WHH79 pKa = 5.5 QRR81 pKa = 3.32
Molecular weight: 8.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.375
IPC2_protein 10.804
IPC_protein 12.384
Toseland 12.559
ProMoST 13.056
Dawson 12.559
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.237
Grimsley 12.588
Solomon 13.042
Lehninger 12.954
Nozaki 12.559
DTASelect 12.544
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 11.974
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 9.1
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3569
0
3569
1177575
39
2501
329.9
35.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.717 ± 0.05
0.812 ± 0.012
6.022 ± 0.032
5.244 ± 0.039
3.791 ± 0.026
8.23 ± 0.034
2.119 ± 0.019
5.319 ± 0.028
4.144 ± 0.028
9.934 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.516 ± 0.021
3.094 ± 0.029
5.062 ± 0.036
3.419 ± 0.025
5.971 ± 0.039
5.709 ± 0.035
5.772 ± 0.049
6.972 ± 0.032
1.435 ± 0.018
2.718 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here