Streptomyces sp. DJ

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5329 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2N5XBT5|A0A2N5XBT5_9ACTN EamA family transporter OS=Streptomyces sp. DJ OX=2067551 GN=C0036_15185 PE=4 SV=1
MM1 pKa = 7.28NKK3 pKa = 9.33HH4 pKa = 5.21RR5 pKa = 11.84VKK7 pKa = 10.77VLAVTAVVMLALTGGRR23 pKa = 11.84GGGGGSSSGGGSGVSKK39 pKa = 10.94SDD41 pKa = 3.83DD42 pKa = 3.4SDD44 pKa = 4.25GGGGCSRR51 pKa = 11.84QSGSSGSHH59 pKa = 6.51HH60 pKa = 7.59DD61 pKa = 4.05SDD63 pKa = 5.89DD64 pKa = 5.25DD65 pKa = 4.88YY66 pKa = 12.13DD67 pKa = 5.27SDD69 pKa = 5.04DD70 pKa = 3.78YY71 pKa = 12.03NYY73 pKa = 10.86DD74 pKa = 3.66DD75 pKa = 5.37SSSEE79 pKa = 3.96QYY81 pKa = 11.62ADD83 pKa = 3.8AEE85 pKa = 4.45AEE87 pKa = 4.18LVSCVTSDD95 pKa = 3.18QPAAGRR101 pKa = 11.84SATVDD106 pKa = 2.91VHH108 pKa = 6.89NPGSGTQTYY117 pKa = 7.64TVEE120 pKa = 4.2VDD122 pKa = 4.64FLAPSDD128 pKa = 3.99GRR130 pKa = 11.84EE131 pKa = 3.75LDD133 pKa = 3.8TNEE136 pKa = 3.78VTVVVAGGGTEE147 pKa = 4.27TVRR150 pKa = 11.84VPMDD154 pKa = 4.33DD155 pKa = 3.24PDD157 pKa = 5.42AYY159 pKa = 11.46VPGTTCEE166 pKa = 4.07LDD168 pKa = 3.27SVYY171 pKa = 11.37

Molecular weight: 17.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2N5XAF0|A0A2N5XAF0_9ACTN Uncharacterized protein OS=Streptomyces sp. DJ OX=2067551 GN=C0036_17780 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84TKK38 pKa = 10.46GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5329

0

5329

1421046

22

1811

266.7

28.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.177 ± 0.045

0.825 ± 0.012

6.057 ± 0.025

6.218 ± 0.032

2.759 ± 0.022

9.703 ± 0.036

2.303 ± 0.019

2.867 ± 0.023

2.172 ± 0.031

10.15 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.805 ± 0.014

1.805 ± 0.017

5.997 ± 0.03

2.625 ± 0.017

8.397 ± 0.043

4.969 ± 0.023

5.834 ± 0.031

8.848 ± 0.033

1.443 ± 0.015

2.047 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski