Amino acid dipepetide frequency for Streptomyces sp. DJ

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.83AlaAla: 20.83 ± 0.193	1.102AlaCys: 1.102 ± 0.033	8.329AlaAsp: 8.329 ± 0.088	9.021AlaGlu: 9.021 ± 0.101	3.416AlaPhe: 3.416 ± 0.053	13.668AlaGly: 13.668 ± 0.12	2.667AlaHis: 2.667 ± 0.045	2.737AlaIle: 2.737 ± 0.049	2.69AlaLys: 2.69 ± 0.061	13.457AlaLeu: 13.457 ± 0.136	2.507AlaMet: 2.507 ± 0.041	1.853AlaAsn: 1.853 ± 0.038	6.794AlaPro: 6.794 ± 0.084	3.411AlaGln: 3.411 ± 0.053	10.356AlaArg: 10.356 ± 0.1	5.698AlaSer: 5.698 ± 0.071	6.037AlaThr: 6.037 ± 0.067	12.983AlaVal: 12.983 ± 0.109	1.782AlaTrp: 1.782 ± 0.035	2.436AlaTyr: 2.436 ± 0.042	0.0AlaXaa: 0.0 ± 0.0
Cys	1.052CysAla: 1.052 ± 0.03	0.087CysCys: 0.087 ± 0.008	0.519CysAsp: 0.519 ± 0.021	0.426CysGlu: 0.426 ± 0.018	0.253CysPhe: 0.253 ± 0.013	0.992CysGly: 0.992 ± 0.029	0.191CysHis: 0.191 ± 0.011	0.197CysIle: 0.197 ± 0.012	0.117CysLys: 0.117 ± 0.01	0.744CysLeu: 0.744 ± 0.023	0.144CysMet: 0.144 ± 0.01	0.158CysAsn: 0.158 ± 0.011	0.517CysPro: 0.517 ± 0.02	0.154CysGln: 0.154 ± 0.01	0.705CysArg: 0.705 ± 0.022	0.493CysSer: 0.493 ± 0.018	0.579CysThr: 0.579 ± 0.02	0.647CysVal: 0.647 ± 0.022	0.122CysTrp: 0.122 ± 0.01	0.152CysTyr: 0.152 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	7.417AspAla: 7.417 ± 0.086	0.453AspCys: 0.453 ± 0.017	3.559AspAsp: 3.559 ± 0.055	4.064AspGlu: 4.064 ± 0.057	1.633AspPhe: 1.633 ± 0.035	6.82AspGly: 6.82 ± 0.09	1.362AspHis: 1.362 ± 0.034	1.73AspIle: 1.73 ± 0.036	1.213AspLys: 1.213 ± 0.038	6.391AspLeu: 6.391 ± 0.075	0.875AspMet: 0.875 ± 0.024	0.973AspAsn: 0.973 ± 0.033	4.621AspPro: 4.621 ± 0.069	1.233AspGln: 1.233 ± 0.031	5.537AspArg: 5.537 ± 0.072	2.637AspSer: 2.637 ± 0.049	3.175AspThr: 3.175 ± 0.049	4.747AspVal: 4.747 ± 0.06	0.974AspTrp: 0.974 ± 0.027	1.153AspTyr: 1.153 ± 0.032	0.0AspXaa: 0.0 ± 0.0
Glu	8.031GluAla: 8.031 ± 0.092	0.414GluCys: 0.414 ± 0.017	3.414GluAsp: 3.414 ± 0.047	4.653GluGlu: 4.653 ± 0.074	1.537GluPhe: 1.537 ± 0.035	4.808GluGly: 4.808 ± 0.059	1.734GluHis: 1.734 ± 0.042	2.317GluIle: 2.317 ± 0.041	1.721GluLys: 1.721 ± 0.046	6.848GluLeu: 6.848 ± 0.086	0.972GluMet: 0.972 ± 0.028	1.165GluAsn: 1.165 ± 0.028	3.503GluPro: 3.503 ± 0.048	2.497GluGln: 2.497 ± 0.043	5.804GluArg: 5.804 ± 0.073	2.552GluSer: 2.552 ± 0.041	3.023GluThr: 3.023 ± 0.053	5.039GluVal: 5.039 ± 0.069	0.832GluTrp: 0.832 ± 0.027	1.317GluTyr: 1.317 ± 0.033	0.0GluXaa: 0.0 ± 0.0
Phe	3.659PheAla: 3.659 ± 0.053	0.274PheCys: 0.274 ± 0.012	2.004PheAsp: 2.004 ± 0.034	1.544PheGlu: 1.544 ± 0.035	0.887PhePhe: 0.887 ± 0.028	3.013PheGly: 3.013 ± 0.051	0.641PheHis: 0.641 ± 0.023	0.623PheIle: 0.623 ± 0.025	0.5PheLys: 0.5 ± 0.019	2.633PheLeu: 2.633 ± 0.052	0.391PheMet: 0.391 ± 0.017	0.557PheAsn: 0.557 ± 0.02	1.427PhePro: 1.427 ± 0.035	0.588PheGln: 0.588 ± 0.019	1.965PheArg: 1.965 ± 0.036	1.529PheSer: 1.529 ± 0.03	2.018PheThr: 2.018 ± 0.039	2.343PheVal: 2.343 ± 0.042	0.421PheTrp: 0.421 ± 0.016	0.571PheTyr: 0.571 ± 0.02	0.0PheXaa: 0.0 ± 0.0
Gly	10.958GlyAla: 10.958 ± 0.101	0.868GlyCys: 0.868 ± 0.027	5.318GlyAsp: 5.318 ± 0.068	5.725GlyGlu: 5.725 ± 0.072	2.859GlyPhe: 2.859 ± 0.043	9.378GlyGly: 9.378 ± 0.117	2.336GlyHis: 2.336 ± 0.041	3.413GlyIle: 3.413 ± 0.049	2.476GlyLys: 2.476 ± 0.061	9.402GlyLeu: 9.402 ± 0.096	2.087GlyMet: 2.087 ± 0.041	1.843GlyAsn: 1.843 ± 0.039	5.483GlyPro: 5.483 ± 0.072	2.517GlyGln: 2.517 ± 0.05	8.829GlyArg: 8.829 ± 0.082	5.412GlySer: 5.412 ± 0.081	6.708GlyThr: 6.708 ± 0.085	7.571GlyVal: 7.571 ± 0.072	1.614GlyTrp: 1.614 ± 0.035	2.236GlyTyr: 2.236 ± 0.044	0.0GlyXaa: 0.0 ± 0.0
His	2.552HisAla: 2.552 ± 0.039	0.206HisCys: 0.206 ± 0.013	1.445HisAsp: 1.445 ± 0.031	1.245HisGlu: 1.245 ± 0.032	0.64HisPhe: 0.64 ± 0.022	2.521HisGly: 2.521 ± 0.048	0.702HisHis: 0.702 ± 0.025	0.594HisIle: 0.594 ± 0.021	0.357HisLys: 0.357 ± 0.014	2.474HisLeu: 2.474 ± 0.049	0.363HisMet: 0.363 ± 0.015	0.407HisAsn: 0.407 ± 0.016	1.806HisPro: 1.806 ± 0.033	0.55HisGln: 0.55 ± 0.019	2.293HisArg: 2.293 ± 0.044	0.993HisSer: 0.993 ± 0.027	1.267HisThr: 1.267 ± 0.029	1.773HisVal: 1.773 ± 0.039	0.375HisTrp: 0.375 ± 0.015	0.464HisTyr: 0.464 ± 0.017	0.0HisXaa: 0.0 ± 0.0
Ile	4.153IleAla: 4.153 ± 0.055	0.273IleCys: 0.273 ± 0.013	2.037IleAsp: 2.037 ± 0.042	1.838IleGlu: 1.838 ± 0.039	0.632IlePhe: 0.632 ± 0.022	3.22IleGly: 3.22 ± 0.056	0.567IleHis: 0.567 ± 0.02	0.771IleIle: 0.771 ± 0.029	0.692IleLys: 0.692 ± 0.025	2.211IleLeu: 2.211 ± 0.045	0.45IleMet: 0.45 ± 0.018	0.709IleAsn: 0.709 ± 0.026	1.518IlePro: 1.518 ± 0.031	0.624IleGln: 0.624 ± 0.022	2.022IleArg: 2.022 ± 0.04	1.486IleSer: 1.486 ± 0.034	2.051IleThr: 2.051 ± 0.036	2.647IleVal: 2.647 ± 0.046	0.306IleTrp: 0.306 ± 0.014	0.463IleTyr: 0.463 ± 0.019	0.0IleXaa: 0.0 ± 0.0
Lys	2.893LysAla: 2.893 ± 0.057	0.118LysCys: 0.118 ± 0.009	1.376LysAsp: 1.376 ± 0.045	1.41LysGlu: 1.41 ± 0.034	0.452LysPhe: 0.452 ± 0.022	1.897LysGly: 1.897 ± 0.039	0.468LysHis: 0.468 ± 0.018	0.775LysIle: 0.775 ± 0.027	0.913LysLys: 0.913 ± 0.037	1.94LysLeu: 1.94 ± 0.045	0.352LysMet: 0.352 ± 0.017	0.585LysAsn: 0.585 ± 0.024	1.226LysPro: 1.226 ± 0.032	0.764LysGln: 0.764 ± 0.028	1.505LysArg: 1.505 ± 0.035	1.095LysSer: 1.095 ± 0.031	1.189LysThr: 1.189 ± 0.035	2.003LysVal: 2.003 ± 0.048	0.235LysTrp: 0.235 ± 0.013	0.522LysTyr: 0.522 ± 0.021	0.0LysXaa: 0.0 ± 0.0
Leu	14.235LeuAla: 14.235 ± 0.13	0.844LeuCys: 0.844 ± 0.023	6.455LeuAsp: 6.455 ± 0.072	5.58LeuGlu: 5.58 ± 0.067	2.664LeuPhe: 2.664 ± 0.054	9.021LeuGly: 9.021 ± 0.092	2.322LeuHis: 2.322 ± 0.046	2.599LeuIle: 2.599 ± 0.05	2.03LeuLys: 2.03 ± 0.043	11.186LeuLeu: 11.186 ± 0.113	1.672LeuMet: 1.672 ± 0.039	1.696LeuAsn: 1.696 ± 0.042	6.471LeuPro: 6.471 ± 0.076	2.298LeuGln: 2.298 ± 0.04	8.815LeuArg: 8.815 ± 0.088	5.134LeuSer: 5.134 ± 0.067	6.151LeuThr: 6.151 ± 0.07	9.2LeuVal: 9.2 ± 0.099	1.21LeuTrp: 1.21 ± 0.033	1.92LeuTyr: 1.92 ± 0.039	0.0LeuXaa: 0.0 ± 0.0
Met	2.26MetAla: 2.26 ± 0.038	0.158MetCys: 0.158 ± 0.011	0.955MetAsp: 0.955 ± 0.029	0.902MetGlu: 0.902 ± 0.026	0.493MetPhe: 0.493 ± 0.018	1.366MetGly: 1.366 ± 0.032	0.392MetHis: 0.392 ± 0.017	0.661MetIle: 0.661 ± 0.023	0.441MetLys: 0.441 ± 0.017	1.797MetLeu: 1.797 ± 0.04	0.308MetMet: 0.308 ± 0.015	0.449MetAsn: 0.449 ± 0.018	1.161MetPro: 1.161 ± 0.028	0.488MetGln: 0.488 ± 0.019	1.529MetArg: 1.529 ± 0.033	1.319MetSer: 1.319 ± 0.029	1.482MetThr: 1.482 ± 0.032	1.358MetVal: 1.358 ± 0.031	0.198MetTrp: 0.198 ± 0.011	0.33MetTyr: 0.33 ± 0.016	0.0MetXaa: 0.0 ± 0.0
Asn	2.136AsnAla: 2.136 ± 0.044	0.18AsnCys: 0.18 ± 0.011	0.987AsnAsp: 0.987 ± 0.027	0.859AsnGlu: 0.859 ± 0.021	0.469AsnPhe: 0.469 ± 0.018	1.907AsnGly: 1.907 ± 0.047	0.424AsnHis: 0.424 ± 0.019	0.658AsnIle: 0.658 ± 0.021	0.438AsnLys: 0.438 ± 0.019	1.728AsnLeu: 1.728 ± 0.034	0.326AsnMet: 0.326 ± 0.014	0.487AsnAsn: 0.487 ± 0.024	1.38AsnPro: 1.38 ± 0.033	0.453AsnGln: 0.453 ± 0.019	1.388AsnArg: 1.388 ± 0.035	0.906AsnSer: 0.906 ± 0.027	1.136AsnThr: 1.136 ± 0.032	1.491AsnVal: 1.491 ± 0.038	0.269AsnTrp: 0.269 ± 0.014	0.425AsnTyr: 0.425 ± 0.017	0.0AsnXaa: 0.0 ± 0.0
Pro	8.368ProAla: 8.368 ± 0.092	0.355ProCys: 0.355 ± 0.016	4.6ProAsp: 4.6 ± 0.068	4.647ProGlu: 4.647 ± 0.064	1.598ProPhe: 1.598 ± 0.029	7.179ProGly: 7.179 ± 0.08	1.363ProHis: 1.363 ± 0.032	1.05ProIle: 1.05 ± 0.028	1.115ProLys: 1.115 ± 0.034	5.136ProLeu: 5.136 ± 0.061	1.041ProMet: 1.041 ± 0.031	0.847ProAsn: 0.847 ± 0.023	3.243ProPro: 3.243 ± 0.057	1.768ProGln: 1.768 ± 0.045	4.101ProArg: 4.101 ± 0.054	3.063ProSer: 3.063 ± 0.052	2.636ProThr: 2.636 ± 0.046	5.727ProVal: 5.727 ± 0.067	0.845ProTrp: 0.845 ± 0.024	1.292ProTyr: 1.292 ± 0.026	0.0ProXaa: 0.0 ± 0.0
Gln	3.473GlnAla: 3.473 ± 0.054	0.168GlnCys: 0.168 ± 0.011	1.418GlnAsp: 1.418 ± 0.034	1.695GlnGlu: 1.695 ± 0.037	0.672GlnPhe: 0.672 ± 0.02	2.096GlnGly: 2.096 ± 0.045	0.644GlnHis: 0.644 ± 0.023	0.97GlnIle: 0.97 ± 0.025	0.631GlnLys: 0.631 ± 0.023	2.728GlnLeu: 2.728 ± 0.041	0.527GlnMet: 0.527 ± 0.019	0.472GlnAsn: 0.472 ± 0.019	1.442GlnPro: 1.442 ± 0.041	1.236GlnGln: 1.236 ± 0.041	2.232GlnArg: 2.232 ± 0.041	1.099GlnSer: 1.099 ± 0.03	1.197GlnThr: 1.197 ± 0.034	2.452GlnVal: 2.452 ± 0.047	0.456GlnTrp: 0.456 ± 0.019	0.639GlnTyr: 0.639 ± 0.024	0.0GlnXaa: 0.0 ± 0.0
Arg	10.173ArgAla: 10.173 ± 0.091	0.65ArgCys: 0.65 ± 0.021	4.478ArgAsp: 4.478 ± 0.062	5.266ArgGlu: 5.266 ± 0.07	2.49ArgPhe: 2.49 ± 0.045	6.229ArgGly: 6.229 ± 0.07	2.145ArgHis: 2.145 ± 0.04	3.416ArgIle: 3.416 ± 0.049	1.725ArgLys: 1.725 ± 0.043	9.157ArgLeu: 9.157 ± 0.101	1.9ArgMet: 1.9 ± 0.042	1.502ArgAsn: 1.502 ± 0.034	5.172ArgPro: 5.172 ± 0.064	2.224ArgGln: 2.224 ± 0.036	8.278ArgArg: 8.278 ± 0.112	4.269ArgSer: 4.269 ± 0.056	5.647ArgThr: 5.647 ± 0.056	6.086ArgVal: 6.086 ± 0.063	1.335ArgTrp: 1.335 ± 0.033	1.828ArgTyr: 1.828 ± 0.037	0.0ArgXaa: 0.0 ± 0.0
Ser	6.261SerAla: 6.261 ± 0.077	0.455SerCys: 0.455 ± 0.018	2.614SerAsp: 2.614 ± 0.046	2.478SerGlu: 2.478 ± 0.046	1.609SerPhe: 1.609 ± 0.035	5.999SerGly: 5.999 ± 0.086	0.979SerHis: 0.979 ± 0.027	1.303SerIle: 1.303 ± 0.03	1.003SerLys: 1.003 ± 0.03	4.788SerLeu: 4.788 ± 0.055	1.044SerMet: 1.044 ± 0.026	0.881SerAsn: 0.881 ± 0.025	3.129SerPro: 3.129 ± 0.051	1.122SerGln: 1.122 ± 0.032	3.808SerArg: 3.808 ± 0.059	2.897SerSer: 2.897 ± 0.053	2.916SerThr: 2.916 ± 0.051	4.403SerVal: 4.403 ± 0.057	0.834SerTrp: 0.834 ± 0.026	1.162SerTyr: 1.162 ± 0.031	0.0SerXaa: 0.0 ± 0.0
Thr	8.498ThrAla: 8.498 ± 0.083	0.442ThrCys: 0.442 ± 0.021	3.455ThrAsp: 3.455 ± 0.057	3.332ThrGlu: 3.332 ± 0.046	1.547ThrPhe: 1.547 ± 0.035	6.96ThrGly: 6.96 ± 0.078	1.093ThrHis: 1.093 ± 0.027	1.385ThrIle: 1.385 ± 0.038	1.046ThrLys: 1.046 ± 0.031	5.117ThrLeu: 5.117 ± 0.055	0.984ThrMet: 0.984 ± 0.026	0.944ThrAsn: 0.944 ± 0.028	3.684ThrPro: 3.684 ± 0.065	1.138ThrGln: 1.138 ± 0.034	3.88ThrArg: 3.88 ± 0.056	2.806ThrSer: 2.806 ± 0.047	3.566ThrThr: 3.566 ± 0.059	6.408ThrVal: 6.408 ± 0.067	0.852ThrTrp: 0.852 ± 0.028	1.199ThrTyr: 1.199 ± 0.032	0.0ThrXaa: 0.0 ± 0.0
Val	10.711ValAla: 10.711 ± 0.115	0.851ValCys: 0.851 ± 0.028	5.345ValAsp: 5.345 ± 0.066	5.458ValGlu: 5.458 ± 0.07	2.574ValPhe: 2.574 ± 0.045	6.776ValGly: 6.776 ± 0.083	2.239ValHis: 2.239 ± 0.044	2.493ValIle: 2.493 ± 0.047	1.839ValLys: 1.839 ± 0.048	10.044ValLeu: 10.044 ± 0.089	1.548ValMet: 1.548 ± 0.034	1.728ValAsn: 1.728 ± 0.038	5.595ValPro: 5.595 ± 0.072	2.076ValGln: 2.076 ± 0.045	7.643ValArg: 7.643 ± 0.091	4.426ValSer: 4.426 ± 0.059	5.391ValThr: 5.391 ± 0.07	9.031ValVal: 9.031 ± 0.102	1.141ValTrp: 1.141 ± 0.029	1.566ValTyr: 1.566 ± 0.033	0.0ValXaa: 0.0 ± 0.0
Trp	1.55TrpAla: 1.55 ± 0.033	0.156TrpCys: 0.156 ± 0.011	0.781TrpAsp: 0.781 ± 0.022	0.787TrpGlu: 0.787 ± 0.025	0.512TrpPhe: 0.512 ± 0.02	1.018TrpGly: 1.018 ± 0.026	0.334TrpHis: 0.334 ± 0.018	0.515TrpIle: 0.515 ± 0.017	0.41TrpLys: 0.41 ± 0.018	1.593TrpLeu: 1.593 ± 0.033	0.281TrpMet: 0.281 ± 0.014	0.397TrpAsn: 0.397 ± 0.02	0.735TrpPro: 0.735 ± 0.02	0.551TrpGln: 0.551 ± 0.02	1.291TrpArg: 1.291 ± 0.03	0.872TrpSer: 0.872 ± 0.026	1.032TrpThr: 1.032 ± 0.029	0.961TrpVal: 0.961 ± 0.026	0.298TrpTrp: 0.298 ± 0.014	0.356TrpTyr: 0.356 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.567TyrAla: 2.567 ± 0.047	0.193TyrCys: 0.193 ± 0.01	1.478TyrAsp: 1.478 ± 0.037	1.251TyrGlu: 1.251 ± 0.028	0.649TyrPhe: 0.649 ± 0.023	2.165TyrGly: 2.165 ± 0.042	0.424TyrHis: 0.424 ± 0.017	0.464TyrIle: 0.464 ± 0.019	0.357TyrLys: 0.357 ± 0.016	2.128TyrLeu: 2.128 ± 0.041	0.276TyrMet: 0.276 ± 0.013	0.396TyrAsn: 0.396 ± 0.016	1.066TyrPro: 1.066 ± 0.031	0.554TyrGln: 0.554 ± 0.02	1.991TyrArg: 1.991 ± 0.038	1.0TyrSer: 1.0 ± 0.03	1.134TyrThr: 1.134 ± 0.027	1.608TyrVal: 1.608 ± 0.033	0.331TyrTrp: 0.331 ± 0.016	0.437TyrTyr: 0.437 ± 0.021	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5329 proteins (1421046 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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