Streptomyces acidiscabies

Taxonomy: cellular organisms; Bacteria;

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7941 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0L0KE36|A0A0L0KE36_9ACTN LysR family transcriptional regulator OS=Streptomyces acidiscabies OX=42234 GN=IQ63_13745 PE=3 SV=1
MM1 pKa = 7.59RR2 pKa = 11.84QTLSRR7 pKa = 11.84GMVAAAAATSILSLCGSPAFADD29 pKa = 3.55SHH31 pKa = 6.16TSGTTAGSPGVLSGNNVEE49 pKa = 4.61VPVDD53 pKa = 3.82VPVNACGNTADD64 pKa = 4.41AAAGFNPTFGNSCGSPAEE82 pKa = 4.11VAQKK86 pKa = 9.84YY87 pKa = 9.99AGYY90 pKa = 10.05GADD93 pKa = 5.82DD94 pKa = 4.8DD95 pKa = 4.69DD96 pKa = 4.72EE97 pKa = 6.61SYY99 pKa = 11.41GGYY102 pKa = 10.42GSYY105 pKa = 11.06GDD107 pKa = 3.98EE108 pKa = 4.44HH109 pKa = 5.99EE110 pKa = 4.94HH111 pKa = 7.2GYY113 pKa = 10.56GAGDD117 pKa = 3.22WGDD120 pKa = 3.44DD121 pKa = 3.28SGYY124 pKa = 9.21GTEE127 pKa = 4.21QPPGGGDD134 pKa = 3.48QPPSGHH140 pKa = 6.52DD141 pKa = 3.37QPPGGGDD148 pKa = 3.51HH149 pKa = 7.21PPGGGDD155 pKa = 3.99QPPTGNDD162 pKa = 3.23QPPGGGEE169 pKa = 4.07EE170 pKa = 4.34CDD172 pKa = 3.67SPGDD176 pKa = 3.86VIGGPDD182 pKa = 3.6SNGEE186 pKa = 4.11TCEE189 pKa = 3.9QPPGGGEE196 pKa = 3.91QPPGGNDD203 pKa = 3.32QPPGGGDD210 pKa = 3.41QPPTGNDD217 pKa = 3.3QPPGGGDD224 pKa = 3.41QPPTGNDD231 pKa = 3.29QPPGGNDD238 pKa = 3.35QPPTGNDD245 pKa = 3.29QPPGGNDD252 pKa = 3.35QPPTGNDD259 pKa = 3.29QPPTGNDD266 pKa = 3.29QPPTGNDD273 pKa = 3.3QPPTGTNQPPTGNDD287 pKa = 3.2QPPAKK292 pKa = 9.85PPVLAHH298 pKa = 6.68TGTSDD303 pKa = 3.49LLAAAGFSAAMIAGGAVLYY322 pKa = 10.57RR323 pKa = 11.84RR324 pKa = 11.84GRR326 pKa = 11.84AASRR330 pKa = 11.84RR331 pKa = 3.88

Molecular weight: 32.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0L0K1R0|A0A0L0K1R0_9ACTN Uncharacterized protein OS=Streptomyces acidiscabies OX=42234 GN=IQ63_24830 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILATRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.88GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7941

0

7941

2604894

33

6471

328.0

35.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.152 ± 0.04

0.767 ± 0.007

6.004 ± 0.019

5.686 ± 0.026

2.796 ± 0.016

9.397 ± 0.028

2.297 ± 0.014

3.128 ± 0.016

2.242 ± 0.022

10.452 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.682 ± 0.012

1.852 ± 0.016

5.923 ± 0.021

2.743 ± 0.016

7.84 ± 0.026

5.149 ± 0.02

6.391 ± 0.027

8.723 ± 0.026

1.559 ± 0.012

2.218 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski