Mycobacterium sp. E740

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Mycobacteriaceae; Mycobacterium; unclassified Mycobacterium

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4184 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1A3BDU2|A0A1A3BDU2_9MYCO Uncharacterized protein OS=Mycobacterium sp. E740 OX=1834149 GN=A5663_07125 PE=4 SV=1
MM1 pKa = 7.78SDD3 pKa = 3.08YY4 pKa = 11.42KK5 pKa = 11.25LFVCVQCGFEE15 pKa = 3.94YY16 pKa = 10.91DD17 pKa = 3.75EE18 pKa = 4.64AKK20 pKa = 10.38GWPEE24 pKa = 4.27DD25 pKa = 4.17GIAPGTRR32 pKa = 11.84WDD34 pKa = 5.68DD35 pKa = 4.13IPDD38 pKa = 3.72DD39 pKa = 4.45WSCPDD44 pKa = 3.66CGAAKK49 pKa = 10.31SDD51 pKa = 3.66FEE53 pKa = 4.37MVEE56 pKa = 4.03VARR59 pKa = 11.84PP60 pKa = 3.22

Molecular weight: 6.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1A3C7Y4|A0A1A3C7Y4_9MYCO Cytochrome OS=Mycobacterium sp. E740 OX=1834149 GN=A5663_13480 PE=3 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVANRR37 pKa = 11.84RR38 pKa = 11.84GKK40 pKa = 10.41GRR42 pKa = 11.84RR43 pKa = 11.84KK44 pKa = 8.73LTAA47 pKa = 3.95

Molecular weight: 5.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4184

0

4184

1367501

29

9988

326.8

35.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.94 ± 0.054

0.813 ± 0.011

6.421 ± 0.029

5.514 ± 0.04

3.067 ± 0.024

8.716 ± 0.031

2.168 ± 0.017

4.163 ± 0.024

2.313 ± 0.028

9.765 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.061 ± 0.019

2.23 ± 0.023

5.756 ± 0.03

2.985 ± 0.02

7.363 ± 0.04

5.33 ± 0.022

5.903 ± 0.029

8.829 ± 0.037

1.51 ± 0.018

2.152 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski