Amino acid dipepetide frequency for Mycobacterium sp. E740

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	19.765AlaAla: 19.765 ± 0.19	0.993AlaCys: 0.993 ± 0.029	8.622AlaAsp: 8.622 ± 0.097	8.08AlaGlu: 8.08 ± 0.09	3.471AlaPhe: 3.471 ± 0.051	11.476AlaGly: 11.476 ± 0.11	2.522AlaHis: 2.522 ± 0.044	5.103AlaIle: 5.103 ± 0.07	3.233AlaLys: 3.233 ± 0.058	12.811AlaLeu: 12.811 ± 0.127	2.925AlaMet: 2.925 ± 0.048	2.613AlaAsn: 2.613 ± 0.05	6.216AlaPro: 6.216 ± 0.094	4.026AlaGln: 4.026 ± 0.057	8.536AlaArg: 8.536 ± 0.087	5.887AlaSer: 5.887 ± 0.064	6.993AlaThr: 6.993 ± 0.079	12.125AlaVal: 12.125 ± 0.122	1.585AlaTrp: 1.585 ± 0.033	2.415AlaTyr: 2.415 ± 0.047	0.0AlaXaa: 0.0 ± 0.0
Cys	1.113CysAla: 1.113 ± 0.029	0.087CysCys: 0.087 ± 0.009	0.547CysAsp: 0.547 ± 0.019	0.437CysGlu: 0.437 ± 0.018	0.217CysPhe: 0.217 ± 0.013	0.911CysGly: 0.911 ± 0.025	0.184CysHis: 0.184 ± 0.012	0.261CysIle: 0.261 ± 0.012	0.139CysLys: 0.139 ± 0.011	0.646CysLeu: 0.646 ± 0.023	0.14CysMet: 0.14 ± 0.01	0.171CysAsn: 0.171 ± 0.012	0.49CysPro: 0.49 ± 0.021	0.222CysGln: 0.222 ± 0.015	0.606CysArg: 0.606 ± 0.021	0.49CysSer: 0.49 ± 0.016	0.459CysThr: 0.459 ± 0.021	0.685CysVal: 0.685 ± 0.023	0.139CysTrp: 0.139 ± 0.012	0.184CysTyr: 0.184 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	7.996AspAla: 7.996 ± 0.097	0.469AspCys: 0.469 ± 0.019	4.678AspAsp: 4.678 ± 0.073	4.447AspGlu: 4.447 ± 0.066	1.838AspPhe: 1.838 ± 0.035	6.178AspGly: 6.178 ± 0.073	1.349AspHis: 1.349 ± 0.032	2.641AspIle: 2.641 ± 0.053	1.456AspLys: 1.456 ± 0.031	5.953AspLeu: 5.953 ± 0.065	1.043AspMet: 1.043 ± 0.028	1.289AspAsn: 1.289 ± 0.03	4.328AspPro: 4.328 ± 0.063	1.639AspGln: 1.639 ± 0.037	4.758AspArg: 4.758 ± 0.057	2.886AspSer: 2.886 ± 0.054	3.103AspThr: 3.103 ± 0.056	5.688AspVal: 5.688 ± 0.068	1.043AspTrp: 1.043 ± 0.028	1.434AspTyr: 1.434 ± 0.03	0.0AspXaa: 0.0 ± 0.0
Glu	6.45GluAla: 6.45 ± 0.075	0.378GluCys: 0.378 ± 0.019	2.607GluAsp: 2.607 ± 0.046	2.733GluGlu: 2.733 ± 0.053	1.961GluPhe: 1.961 ± 0.04	3.424GluGly: 3.424 ± 0.049	1.618GluHis: 1.618 ± 0.035	2.752GluIle: 2.752 ± 0.05	1.536GluLys: 1.536 ± 0.038	6.917GluLeu: 6.917 ± 0.088	1.173GluMet: 1.173 ± 0.031	1.165GluAsn: 1.165 ± 0.031	3.204GluPro: 3.204 ± 0.06	2.341GluGln: 2.341 ± 0.046	4.689GluArg: 4.689 ± 0.068	2.788GluSer: 2.788 ± 0.043	2.815GluThr: 2.815 ± 0.04	4.622GluVal: 4.622 ± 0.059	0.79GluTrp: 0.79 ± 0.025	1.174GluTyr: 1.174 ± 0.029	0.0GluXaa: 0.0 ± 0.0
Phe	4.015PheAla: 4.015 ± 0.059	0.309PheCys: 0.309 ± 0.015	2.633PheAsp: 2.633 ± 0.051	1.747PheGlu: 1.747 ± 0.037	1.024PhePhe: 1.024 ± 0.031	3.446PheGly: 3.446 ± 0.06	0.619PheHis: 0.619 ± 0.021	1.019PheIle: 1.019 ± 0.028	0.568PheLys: 0.568 ± 0.024	2.481PheLeu: 2.481 ± 0.055	0.514PheMet: 0.514 ± 0.021	0.785PheAsn: 0.785 ± 0.023	1.403PhePro: 1.403 ± 0.035	0.679PheGln: 0.679 ± 0.019	1.771PheArg: 1.771 ± 0.034	1.71PheSer: 1.71 ± 0.035	2.079PheThr: 2.079 ± 0.042	2.706PheVal: 2.706 ± 0.042	0.468PheTrp: 0.468 ± 0.02	0.698PheTyr: 0.698 ± 0.024	0.0PheXaa: 0.0 ± 0.0
Gly	10.032GlyAla: 10.032 ± 0.093	0.812GlyCys: 0.812 ± 0.027	5.136GlyAsp: 5.136 ± 0.069	4.635GlyGlu: 4.635 ± 0.069	3.049GlyPhe: 3.049 ± 0.047	7.604GlyGly: 7.604 ± 0.101	1.98GlyHis: 1.98 ± 0.039	3.939GlyIle: 3.939 ± 0.061	2.286GlyLys: 2.286 ± 0.039	8.382GlyLeu: 8.382 ± 0.082	2.113GlyMet: 2.113 ± 0.043	1.887GlyAsn: 1.887 ± 0.043	4.497GlyPro: 4.497 ± 0.067	2.791GlyGln: 2.791 ± 0.052	6.425GlyArg: 6.425 ± 0.075	5.041GlySer: 5.041 ± 0.068	4.984GlyThr: 4.984 ± 0.068	7.608GlyVal: 7.608 ± 0.085	1.622GlyTrp: 1.622 ± 0.039	2.341GlyTyr: 2.341 ± 0.048	0.0GlyXaa: 0.0 ± 0.0
His	2.357HisAla: 2.357 ± 0.045	0.211HisCys: 0.211 ± 0.013	1.379HisAsp: 1.379 ± 0.029	1.111HisGlu: 1.111 ± 0.033	0.673HisPhe: 0.673 ± 0.025	2.075HisGly: 2.075 ± 0.037	0.67HisHis: 0.67 ± 0.028	0.854HisIle: 0.854 ± 0.025	0.384HisLys: 0.384 ± 0.016	2.081HisLeu: 2.081 ± 0.046	0.356HisMet: 0.356 ± 0.016	0.467HisAsn: 0.467 ± 0.019	1.637HisPro: 1.637 ± 0.035	0.619HisGln: 0.619 ± 0.023	1.871HisArg: 1.871 ± 0.039	1.116HisSer: 1.116 ± 0.027	1.161HisThr: 1.161 ± 0.028	1.746HisVal: 1.746 ± 0.036	0.366HisTrp: 0.366 ± 0.015	0.546HisTyr: 0.546 ± 0.024	0.0HisXaa: 0.0 ± 0.0
Ile	6.353IleAla: 6.353 ± 0.07	0.416IleCys: 0.416 ± 0.021	3.393IleAsp: 3.393 ± 0.05	2.682IleGlu: 2.682 ± 0.05	1.038IlePhe: 1.038 ± 0.031	4.429IleGly: 4.429 ± 0.067	0.767IleHis: 0.767 ± 0.023	1.405IleIle: 1.405 ± 0.038	0.933IleLys: 0.933 ± 0.025	2.847IleLeu: 2.847 ± 0.051	0.572IleMet: 0.572 ± 0.021	1.106IleAsn: 1.106 ± 0.028	2.301IlePro: 2.301 ± 0.046	0.899IleGln: 0.899 ± 0.025	2.614IleArg: 2.614 ± 0.048	2.241IleSer: 2.241 ± 0.044	2.627IleThr: 2.627 ± 0.041	3.722IleVal: 3.722 ± 0.056	0.489IleTrp: 0.489 ± 0.017	0.793IleTyr: 0.793 ± 0.026	0.0IleXaa: 0.0 ± 0.0
Lys	2.856LysAla: 2.856 ± 0.053	0.125LysCys: 0.125 ± 0.009	1.183LysAsp: 1.183 ± 0.037	1.103LysGlu: 1.103 ± 0.036	0.665LysPhe: 0.665 ± 0.024	1.631LysGly: 1.631 ± 0.039	0.509LysHis: 0.509 ± 0.021	1.054LysIle: 1.054 ± 0.029	0.768LysLys: 0.768 ± 0.031	2.391LysLeu: 2.391 ± 0.049	0.509LysMet: 0.509 ± 0.021	0.57LysAsn: 0.57 ± 0.022	1.55LysPro: 1.55 ± 0.035	0.78LysGln: 0.78 ± 0.027	1.783LysArg: 1.783 ± 0.042	1.286LysSer: 1.286 ± 0.035	1.435LysThr: 1.435 ± 0.034	2.094LysVal: 2.094 ± 0.044	0.349LysTrp: 0.349 ± 0.017	0.497LysTyr: 0.497 ± 0.019	0.0LysXaa: 0.0 ± 0.0
Leu	13.755LeuAla: 13.755 ± 0.108	0.802LeuCys: 0.802 ± 0.023	6.644LeuAsp: 6.644 ± 0.093	4.633LeuGlu: 4.633 ± 0.069	2.735LeuPhe: 2.735 ± 0.05	8.513LeuGly: 8.513 ± 0.077	2.018LeuHis: 2.018 ± 0.042	4.072LeuIle: 4.072 ± 0.061	2.005LeuLys: 2.005 ± 0.046	9.626LeuLeu: 9.626 ± 0.103	1.781LeuMet: 1.781 ± 0.044	2.141LeuAsn: 2.141 ± 0.046	5.684LeuPro: 5.684 ± 0.07	2.63LeuGln: 2.63 ± 0.05	7.602LeuArg: 7.602 ± 0.08	5.631LeuSer: 5.631 ± 0.066	6.222LeuThr: 6.222 ± 0.071	8.296LeuVal: 8.296 ± 0.087	1.227LeuTrp: 1.227 ± 0.034	1.633LeuTyr: 1.633 ± 0.036	0.0LeuXaa: 0.0 ± 0.0
Met	2.657MetAla: 2.657 ± 0.047	0.203MetCys: 0.203 ± 0.013	0.883MetAsp: 0.883 ± 0.026	0.729MetGlu: 0.729 ± 0.023	0.65MetPhe: 0.65 ± 0.027	1.491MetGly: 1.491 ± 0.037	0.459MetHis: 0.459 ± 0.016	0.878MetIle: 0.878 ± 0.026	0.471MetLys: 0.471 ± 0.019	2.078MetLeu: 2.078 ± 0.046	0.42MetMet: 0.42 ± 0.018	0.522MetAsn: 0.522 ± 0.019	1.246MetPro: 1.246 ± 0.032	0.578MetGln: 0.578 ± 0.019	1.639MetArg: 1.639 ± 0.033	1.55MetSer: 1.55 ± 0.031	1.873MetThr: 1.873 ± 0.039	1.655MetVal: 1.655 ± 0.036	0.287MetTrp: 0.287 ± 0.013	0.346MetTyr: 0.346 ± 0.017	0.0MetXaa: 0.0 ± 0.0
Asn	2.664AsnAla: 2.664 ± 0.057	0.182AsnCys: 0.182 ± 0.012	1.291AsnAsp: 1.291 ± 0.031	1.07AsnGlu: 1.07 ± 0.031	0.682AsnPhe: 0.682 ± 0.026	2.118AsnGly: 2.118 ± 0.041	0.448AsnHis: 0.448 ± 0.016	0.927AsnIle: 0.927 ± 0.028	0.507AsnLys: 0.507 ± 0.019	2.08AsnLeu: 2.08 ± 0.037	0.427AsnMet: 0.427 ± 0.019	0.63AsnAsn: 0.63 ± 0.036	1.716AsnPro: 1.716 ± 0.038	0.639AsnGln: 0.639 ± 0.025	1.654AsnArg: 1.654 ± 0.033	1.139AsnSer: 1.139 ± 0.036	1.332AsnThr: 1.332 ± 0.038	1.809AsnVal: 1.809 ± 0.039	0.401AsnTrp: 0.401 ± 0.017	0.589AsnTyr: 0.589 ± 0.018	0.0AsnXaa: 0.0 ± 0.0
Pro	7.133ProAla: 7.133 ± 0.106	0.309ProCys: 0.309 ± 0.016	4.38ProAsp: 4.38 ± 0.059	3.784ProGlu: 3.784 ± 0.061	1.686ProPhe: 1.686 ± 0.037	5.487ProGly: 5.487 ± 0.07	1.204ProHis: 1.204 ± 0.033	2.157ProIle: 2.157 ± 0.04	1.411ProLys: 1.411 ± 0.033	4.871ProLeu: 4.871 ± 0.056	1.21ProMet: 1.21 ± 0.032	1.295ProAsn: 1.295 ± 0.028	3.721ProPro: 3.721 ± 0.098	1.866ProGln: 1.866 ± 0.038	3.425ProArg: 3.425 ± 0.054	3.069ProSer: 3.069 ± 0.053	3.395ProThr: 3.395 ± 0.054	5.137ProVal: 5.137 ± 0.077	0.831ProTrp: 0.831 ± 0.028	1.189ProTyr: 1.189 ± 0.033	0.0ProXaa: 0.0 ± 0.0
Gln	3.53GlnAla: 3.53 ± 0.059	0.238GlnCys: 0.238 ± 0.014	1.227GlnAsp: 1.227 ± 0.032	1.211GlnGlu: 1.211 ± 0.034	0.999GlnPhe: 0.999 ± 0.028	1.971GlnGly: 1.971 ± 0.043	0.763GlnHis: 0.763 ± 0.023	1.436GlnIle: 1.436 ± 0.031	0.679GlnLys: 0.679 ± 0.024	3.495GlnLeu: 3.495 ± 0.058	0.698GlnMet: 0.698 ± 0.021	0.645GlnAsn: 0.645 ± 0.02	1.862GlnPro: 1.862 ± 0.04	1.315GlnGln: 1.315 ± 0.037	2.88GlnArg: 2.88 ± 0.052	1.529GlnSer: 1.529 ± 0.029	1.647GlnThr: 1.647 ± 0.035	2.445GlnVal: 2.445 ± 0.052	0.584GlnTrp: 0.584 ± 0.02	0.694GlnTyr: 0.694 ± 0.024	0.0GlnXaa: 0.0 ± 0.0
Arg	8.161ArgAla: 8.161 ± 0.094	0.627ArgCys: 0.627 ± 0.024	4.422ArgAsp: 4.422 ± 0.067	4.097ArgGlu: 4.097 ± 0.062	2.507ArgPhe: 2.507 ± 0.043	5.239ArgGly: 5.239 ± 0.062	1.792ArgHis: 1.792 ± 0.037	3.51ArgIle: 3.51 ± 0.045	1.667ArgLys: 1.667 ± 0.042	7.699ArgLeu: 7.699 ± 0.092	1.874ArgMet: 1.874 ± 0.035	1.672ArgAsn: 1.672 ± 0.035	4.081ArgPro: 4.081 ± 0.059	2.302ArgGln: 2.302 ± 0.042	6.736ArgArg: 6.736 ± 0.102	3.949ArgSer: 3.949 ± 0.055	4.222ArgThr: 4.222 ± 0.054	5.653ArgVal: 5.653 ± 0.07	1.432ArgTrp: 1.432 ± 0.033	1.992ArgTyr: 1.992 ± 0.039	0.0ArgXaa: 0.0 ± 0.0
Ser	7.014SerAla: 7.014 ± 0.083	0.404SerCys: 0.404 ± 0.018	3.315SerAsp: 3.315 ± 0.05	2.689SerGlu: 2.689 ± 0.045	1.604SerPhe: 1.604 ± 0.039	5.462SerGly: 5.462 ± 0.065	1.03SerHis: 1.03 ± 0.029	2.064SerIle: 2.064 ± 0.044	1.197SerLys: 1.197 ± 0.032	4.862SerLeu: 4.862 ± 0.053	1.345SerMet: 1.345 ± 0.031	1.114SerAsn: 1.114 ± 0.028	3.034SerPro: 3.034 ± 0.052	1.46SerGln: 1.46 ± 0.032	3.788SerArg: 3.788 ± 0.051	2.996SerSer: 2.996 ± 0.051	3.221SerThr: 3.221 ± 0.05	4.614SerVal: 4.614 ± 0.055	0.849SerTrp: 0.849 ± 0.026	1.235SerTyr: 1.235 ± 0.026	0.0SerXaa: 0.0 ± 0.0
Thr	7.836ThrAla: 7.836 ± 0.088	0.397ThrCys: 0.397 ± 0.018	3.68ThrAsp: 3.68 ± 0.054	3.199ThrGlu: 3.199 ± 0.045	1.913ThrPhe: 1.913 ± 0.039	5.716ThrGly: 5.716 ± 0.072	1.084ThrHis: 1.084 ± 0.027	2.283ThrIle: 2.283 ± 0.041	1.311ThrLys: 1.311 ± 0.036	5.43ThrLeu: 5.43 ± 0.067	1.217ThrMet: 1.217 ± 0.03	1.233ThrAsn: 1.233 ± 0.034	3.716ThrPro: 3.716 ± 0.052	1.498ThrGln: 1.498 ± 0.032	3.544ThrArg: 3.544 ± 0.054	3.131ThrSer: 3.131 ± 0.05	3.831ThrThr: 3.831 ± 0.084	5.855ThrVal: 5.855 ± 0.069	0.839ThrTrp: 0.839 ± 0.027	1.313ThrTyr: 1.313 ± 0.029	0.0ThrXaa: 0.0 ± 0.0
Val	11.692ValAla: 11.692 ± 0.134	0.766ValCys: 0.766 ± 0.025	5.984ValAsp: 5.984 ± 0.066	4.926ValGlu: 4.926 ± 0.056	2.64ValPhe: 2.64 ± 0.048	6.973ValGly: 6.973 ± 0.072	1.823ValHis: 1.823 ± 0.033	3.923ValIle: 3.923 ± 0.052	1.854ValLys: 1.854 ± 0.042	8.971ValLeu: 8.971 ± 0.098	1.634ValMet: 1.634 ± 0.041	2.126ValAsn: 2.126 ± 0.046	4.769ValPro: 4.769 ± 0.07	2.208ValGln: 2.208 ± 0.042	6.093ValArg: 6.093 ± 0.08	4.743ValSer: 4.743 ± 0.054	5.517ValThr: 5.517 ± 0.062	8.928ValVal: 8.928 ± 0.099	1.09ValTrp: 1.09 ± 0.027	1.628ValTyr: 1.628 ± 0.038	0.0ValXaa: 0.0 ± 0.0
Trp	1.563TrpAla: 1.563 ± 0.037	0.155TrpCys: 0.155 ± 0.012	0.801TrpAsp: 0.801 ± 0.025	0.679TrpGlu: 0.679 ± 0.024	0.524TrpPhe: 0.524 ± 0.021	0.96TrpGly: 0.96 ± 0.028	0.369TrpHis: 0.369 ± 0.016	0.659TrpIle: 0.659 ± 0.024	0.322TrpLys: 0.322 ± 0.014	1.741TrpLeu: 1.741 ± 0.04	0.357TrpMet: 0.357 ± 0.016	0.381TrpAsn: 0.381 ± 0.018	0.861TrpPro: 0.861 ± 0.028	0.68TrpGln: 0.68 ± 0.026	1.297TrpArg: 1.297 ± 0.033	0.965TrpSer: 0.965 ± 0.026	0.93TrpThr: 0.93 ± 0.025	1.178TrpVal: 1.178 ± 0.032	0.349TrpTrp: 0.349 ± 0.019	0.334TrpTyr: 0.334 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.456TyrAla: 2.456 ± 0.034	0.245TyrCys: 0.245 ± 0.012	1.411TyrAsp: 1.411 ± 0.035	1.147TyrGlu: 1.147 ± 0.03	0.799TyrPhe: 0.799 ± 0.024	2.057TyrGly: 2.057 ± 0.043	0.472TyrHis: 0.472 ± 0.019	0.69TyrIle: 0.69 ± 0.027	0.408TyrLys: 0.408 ± 0.017	2.289TyrLeu: 2.289 ± 0.044	0.305TyrMet: 0.305 ± 0.013	0.493TyrAsn: 0.493 ± 0.019	1.242TyrPro: 1.242 ± 0.035	0.678TyrGln: 0.678 ± 0.02	1.921TyrArg: 1.921 ± 0.037	1.152TyrSer: 1.152 ± 0.029	1.182TyrThr: 1.182 ± 0.034	1.721TyrVal: 1.721 ± 0.037	0.367TyrTrp: 0.367 ± 0.017	0.482TyrTyr: 0.482 ± 0.023	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4184 proteins (1367501 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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