Sphingomonas lenta

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3520 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2A2SAY7|A0A2A2SAY7_9SPHN MBL fold metallo-hydrolase OS=Sphingomonas lenta OX=1141887 GN=CKY28_17645 PE=4 SV=1
MM1 pKa = 7.87PLGDD5 pKa = 3.89NSSLYY10 pKa = 7.24TTSEE14 pKa = 3.92RR15 pKa = 11.84VDD17 pKa = 2.98IAFNNSNDD25 pKa = 3.51EE26 pKa = 4.05YY27 pKa = 11.28DD28 pKa = 3.64TFDD31 pKa = 3.53YY32 pKa = 11.1SALAVSFYY40 pKa = 10.7FGNGTTADD48 pKa = 3.77DD49 pKa = 3.82SLVNFGKK56 pKa = 10.34NDD58 pKa = 3.31TVVNFKK64 pKa = 10.89QIFDD68 pKa = 4.01GNGDD72 pKa = 3.83GIVAFGGNGVLDD84 pKa = 3.62IDD86 pKa = 4.06RR87 pKa = 11.84TGSGGNRR94 pKa = 11.84AGEE97 pKa = 3.98AQITVNSGDD106 pKa = 3.76ADD108 pKa = 3.66ILALRR113 pKa = 11.84YY114 pKa = 9.65LGSKK118 pKa = 10.52GGDD121 pKa = 3.24PNGNGGHH128 pKa = 6.16VYY130 pKa = 11.1ADD132 pKa = 3.08ASTRR136 pKa = 11.84LAGFTEE142 pKa = 4.7GTVSNDD148 pKa = 2.94NFNAATGNFTYY159 pKa = 9.73FYY161 pKa = 10.07DD162 pKa = 3.81TALGLNLGGDD172 pKa = 4.44TITGFGAGDD181 pKa = 3.97RR182 pKa = 11.84IVTTTRR188 pKa = 11.84IHH190 pKa = 6.38NGPDD194 pKa = 2.86AGALITFGANGVLDD208 pKa = 4.86LPGEE212 pKa = 4.1MDD214 pKa = 3.98GVKK217 pKa = 10.65GDD219 pKa = 3.68IGPAQGGQIDD229 pKa = 3.93FGGSVSSLVLLNTVAGDD246 pKa = 3.62GVTYY250 pKa = 10.12YY251 pKa = 10.89YY252 pKa = 10.99YY253 pKa = 11.29GVAA256 pKa = 4.29

Molecular weight: 26.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2A2SKF7|A0A2A2SKF7_9SPHN NADH-quinone oxidoreductase subunit A OS=Sphingomonas lenta OX=1141887 GN=nuoA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84AVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3520

0

3520

1111885

37

2581

315.9

34.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.936 ± 0.074

0.684 ± 0.011

6.021 ± 0.037

5.683 ± 0.043

3.423 ± 0.028

9.25 ± 0.052

1.873 ± 0.021

3.986 ± 0.03

2.324 ± 0.03

10.08 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.134 ± 0.021

2.245 ± 0.029

5.649 ± 0.036

2.934 ± 0.029

8.587 ± 0.051

4.639 ± 0.027

5.146 ± 0.034

7.87 ± 0.033

1.421 ± 0.021

2.113 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski