Moraxella phage Mcat27
Average proteome isoelectric point is 6.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0R6PFA5|A0A0R6PFA5_9CAUD Uncharacterized protein OS=Moraxella phage Mcat27 OX=1647543 PE=4 SV=1
MM1 pKa = 6.35 TQKK4 pKa = 8.9 ITLEE8 pKa = 4.19 MVKK11 pKa = 9.52 HH12 pKa = 5.15 QCRR15 pKa = 11.84 IDD17 pKa = 4.23 HH18 pKa = 7.34 DD19 pKa = 5.06 DD20 pKa = 3.86 EE21 pKa = 7.61 DD22 pKa = 5.29 DD23 pKa = 5.08 LLMHH27 pKa = 6.72 YY28 pKa = 10.75 LNAALIHH35 pKa = 6.2 ASNYY39 pKa = 9.48 IDD41 pKa = 4.47 GLLDD45 pKa = 3.61 EE46 pKa = 5.83 SNLAVQQAVLLLVGHH61 pKa = 7.35 WYY63 pKa = 10.33 DD64 pKa = 4.08 NRR66 pKa = 11.84 EE67 pKa = 4.03 AVNNDD72 pKa = 2.91 YY73 pKa = 8.08 QTPQSIPFGFEE84 pKa = 3.73 ALLQPYY90 pKa = 10.05 RR91 pKa = 11.84 NLGVV95 pKa = 3.22
Molecular weight: 10.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.249
IPC2_protein 4.558
IPC_protein 4.444
Toseland 4.304
ProMoST 4.609
Dawson 4.444
Bjellqvist 4.584
Wikipedia 4.368
Rodwell 4.304
Grimsley 4.215
Solomon 4.444
Lehninger 4.393
Nozaki 4.571
DTASelect 4.787
Thurlkill 4.329
EMBOSS 4.393
Sillero 4.596
Patrickios 2.028
IPC_peptide 4.444
IPC2_peptide 4.584
IPC2.peptide.svr19 4.483
Protein with the highest isoelectric point:
>tr|A0A0R6PJX0|A0A0R6PJX0_9CAUD Uncharacterized protein OS=Moraxella phage Mcat27 OX=1647543 PE=4 SV=1
MM1 pKa = 7.02 YY2 pKa = 10.4 GGWSHH7 pKa = 6.29 EE8 pKa = 4.38 KK9 pKa = 9.93 IAVRR13 pKa = 11.84 YY14 pKa = 9.1 LSAIGYY20 pKa = 8.4 RR21 pKa = 11.84 GKK23 pKa = 10.63 SRR25 pKa = 11.84 ASRR28 pKa = 11.84 HH29 pKa = 5.37 DD30 pKa = 3.4 VRR32 pKa = 11.84 SALIKK37 pKa = 10.73 AEE39 pKa = 4.06 SFLAGLLHH47 pKa = 6.76 KK48 pKa = 10.43 LYY50 pKa = 11.03 SFTT53 pKa = 4.73
Molecular weight: 6.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.26
IPC2_protein 9.911
IPC_protein 10.394
Toseland 10.292
ProMoST 10.043
Dawson 10.496
Bjellqvist 10.204
Wikipedia 10.701
Rodwell 10.833
Grimsley 10.584
Solomon 10.54
Lehninger 10.511
Nozaki 10.248
DTASelect 10.204
Thurlkill 10.335
EMBOSS 10.687
Sillero 10.409
Patrickios 10.599
IPC_peptide 10.54
IPC2_peptide 8.975
IPC2.peptide.svr19 8.609
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
55
0
55
12196
38
1175
221.7
24.86
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.314 ± 0.587
1.074 ± 0.171
6.379 ± 0.273
5.92 ± 0.241
3.583 ± 0.203
6.437 ± 0.355
2.558 ± 0.252
6.445 ± 0.249
6.797 ± 0.257
8.814 ± 0.301
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.73 ± 0.215
5.002 ± 0.302
3.288 ± 0.237
4.526 ± 0.204
4.78 ± 0.249
6.617 ± 0.531
6.018 ± 0.409
5.904 ± 0.277
1.304 ± 0.12
3.509 ± 0.281
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here